Comment: 0-22 hr AEL
GAAGTTTTCTCGGGTTTAACGCTTTTAATCCGTCGTGGGTGTCAATATCCTGTGTATAAAAATAATTCCTAAGGCAACTG TGCACTTGGCACACATCTTCATATCGAGTTAGCCCAATTTAAAGCTCCAGATCGGCCGCTAATATGTCGGAAAGATCGAA AGGTGACGTGTCCGCTCGCGTAAACAACAACGCAAACCAGACGCGTGTCGTTAAAAATCCGCTTCTGAGCGCCTCCGTGA CCGGCCTGAGTTTCGACGACTTCCCCAACAAGCCGCAGCTCCTGCCAGCCGCCCCGCCCGTGGTCCATGCCACGCCTCCA GCGGCGCCCGTCCTCATCGCTGGCATCTTGAATCGCGAACCGAAATCATTGGTCACCGTTGGTCAGCCATCGAGCATTGA CGATAGCGAGGCGCTCGACGAGATTAACTTGAATACCAGCAGCGAGGATTCCGTTGGCGCAGGTGGATCGAGTCCATATC TAGGAGCAGGCGATCAAAACGCCAATCCTCTGTTGGAACCACCAACGGAAGCTGGAAACGATTCGTTGCTGAGCCAGGCG GCCTCCTCCTTTACTGCCCTGCCTGCGGTGGCATCCAATGTGTTTTCCACCTTCTCGAAGCGGATCTACGCCGGCAGCAG GGAGACCTCGGAGGAGCCCAAACTAGACATACAGCCCTCTTACATTCAGCAGGCCAGCTACGTTCAGCCAGTAGCTCCAG TGCCAGCTCCCTTTTATGCAGCACCGCCAGCGGCCGCAAACGAAGTGGTCGCCCCCGAACCACCCAAGTTCTACGCACCT ACTGAGGTTCCGCCACCGAATGTAGGAGTCCCTGCACCACCGCCTACATCGGGAAATCCAAACACATACAGATTCACCGC CAGGAAGAAGCTATACGCTCCCATTCCTGGATTATCCGAGCAGTCCGGACAACCTGCACAACAGCCACAGGTTCCTCAGG CTCCCCAAGCGGCTCCATCATTCCAGACGCCACCATATCCACCACAGCTAACGTACGCAGCAAACCCTGCTCCCTTTGAC ATATCAGCGCAGCCAGCTGCAATTCCCGCAGAGGAACGCAAATCTGGTGGAGGACTCTTCTCTTTGACCAGTCTCGTGCC CACAGGAGTGCTTCAAAATATCACAGGAATTGTTCAATCAGCCACTGGGCGAAGCAGTGAACCAGAACACACTAACGTCC AGCAAGCAGGAGGCTACCTAGACATTACAACAGCATCAGCACCAGCTCCATCGACCAACTTATTTGGTGGACCGAATTCA ACCTTTCCGCCGGGAGGAAATTATTTCGTACCGGGAGCACCTCCATCATTACCCTCAACAGGGGTAGTAGGCCCCCCTTC GGTCGGAAACTTCTTCGCGCCGGCTGCTCCATTGCCAGTAGCACAAACAGCTGCTCCACCAGCTGTCGGAGCCTTCTTCA CATCTGCTGCCCCAAGTGTTACGCCTTCAGTTTTTACTCCAGGAACAGTTCCACCTCTTGCACCGCAGCCTTTTCAGTCA GCAACTGTAAACCAATCTGCATTGCCACCAAACAATCCGCCGACCGCATCTGCAGTAGGAGAACTCTTTGTTCCAGCACC AACGACAATCGCCCAAGGAACTTCACCTGCAGTTCCATTTGTTGACACACAAGCTGTTCCAACAATACCTTTAACAGTTC CACCAGGCAATCCTCCTCCAACATCGACACTTGGAGGATTCTTTACCACAGGTGAAGTTGCACCTGTTGTTGCGCCATTT GGAACACAAGCGTCACCAGCAACACAATCTGCAGGATTCTTTAATCCAGGAGAAGTTCGTCCTAATGTCCCGTTAGCCGA ACCTGTTCCAACTATACCGATTACATCATCAGGTGTTCCACCTATAGGATTATTTCCTCCAGCACCGGCACAGCCTGGTG TCCAAACTGCTTCAAATCCGAATCCCTTGCCAGGAGCAGCTGCACCGCCACCAGCCGCTGGGCAAGCTTCATATCGTCTC CAGAAGGGAACTCGTCTTTACAAGAGTCCATTGACGCCCCAGGAAACCGCAACATCTTCGGGATTCGCCGCTCCTCTGCC CCCCACATCTGTAACTCCAGCAATTTTTAATCCATTCGGAGCACCGGCACCAGGATCTTTCAATTCAGTAGGCCATCTAG AGGGTCAATCCAGCCAAGGAGTTCCTTTCTTTACGCCGCCAGTGCAGAATCCACCAGGACAAGTAGCGGCTCCAACTGTT GCTGTTCCGCAATCAGCACCTATTGCCCCTCCACCAGGGACATCAAGCACAAGTGTTGCTCCTCCGCTAACAGCATCTAT AGCCAATCCTCCTCCAACATCTGGCTTTGCAAGTGTACCCCTCTTCGCTGCACCAACAATATCCTCAACGCCTATTCCTT TCTTCAATCCTCAACCAACAGGAGCAGAAGTAACAACTGGTTTGAGCCAACAGGAAAACGAAGCTACAGCATCGACAACT TTGGAAGGTCTAGTGCAAGGACCTGCTCCAACACTCTCTGCAAGTCAATTCTTTGATACGCCCTTGCCAACAGGAACCCA ACCGGAGAATCAAGTAGCAAGTGTTTCCTCGGAAAGAGAAAATCTTGTACAGGAGCCAATATCGAACCAAGGAATTCCAT TCTTTGCGCCGCCGCAAACGAATATTTTTACACCACAGCTTCAGCAAGAGTTCATAGCACCAGCAGAGGATTCCAAGCGA TCCGAGATTCAAGAAGAATTCTTATCAGCACCACCACAAGAATCTAATCTATCAACCGAACTTAGAGAAGATACACACAC TGGTCCACCCGTAATCGAACCCGTTTTGGAAGAAACCCCAGCAAGTAGCTCGGAATCGAGTGTTCCGCCCGCAAGTAAGG TAGATCAAGAAGATATAGTCTACCCAGAACCATCTGCGCCGGTGGAACAAGAGATTCCATTATTCGTTCCAGTGCCAGGT TTGCCGGGACTAAGTCAGGACATACCACTATATCCACCACCACCAACTGGTTCCACCACTGCCAGCTCGATTTTTGCTGC GCCACCAGTGGCTTCAAGTGCTCCACTTTTTGTTCCTGTTCCTGGTCCTCCGTCGGTTTCATCGTTATTTTCCCCCTCTC AAGTGGATTCTACTTCTTCGTTATTTGCTCCGCCACCAACATCTGCAAATACAACAATACTAACACCGGCTCCGGATACG ACTTCTACGTTGTTTGCCAACAACATACCAACAACAACACCATCCATACCAGATATAGTTCCAACTACATCGGCTGAGCT GGTTGACGCACCTCCTGGTTTCCAGGGATCATCAGACGACACCGCAACCAATCTATTTCAGGGAAACACCGAAACCCAAA CAGTTTTTAGTCCATTTGCACAAACTAAGGCTACACCTTTGGTTCAGGAAACAAAACCCGAATTGGTTCCACCTCCAATT GGATTCTTTGCGCCGAAAAGCGAGTCGGAAGTTCTTAGCCAGCCACCACTTGCTCCCTCGCCACTGCAAAGCTTCTTCAG CACAGCACCAGAAACAGCAACAACCACCGACTTCAATTTCTTCGGAAGCCCAGCACCCGGCAGCGTGTTTCCTGATTTGC CAGTGCAGCAGGGAATCGCACCGCCACCATTGGCACTTGAGTCACCGCCACAGAATAGTGTGTCCAATATAGCCACATCT GCTATAGGATTCGATTTGTTAAACCAGACTCAGCAAGCTTCAAGTTTAGTCGAAAACTCAAATCAAAATTCAAGTGTCAA CTCTTTCGACGGCTATTTTGGTGGTCCTAGTGCATCTGCGGCACAAACATTCGCAAACGAGACAACTAATGCACTTGTTG CTCCAGCTATAAACTGCGATCCCGTCAATTTCTTCGATCAGGTTCCTCAAACCCAGTCGCAAATCCAGCAAGCAAACGAG GATCAGCGCATTCAGAACTTCTTCAACAATCCACCGTTGCAGGATCAACCCGCTGTACCAGGTGAACTCAAATACGACAT TGTCCACAGCGGAGTGGCTGTTAAGCAGCTGCAAGAGCGCTCACAGACGCCCGTTTCAAATCTTGTCGAGCCACCTTCGT CTGCCTGTTCGGAATTCTCGACCCTTGCTCCAGCACCAACACAAACCGATCGCCAATCGGGCGACGATCTACTCCAACAG CATCTGGGCGAATTGCCCGAGGATATCCTCAGAGAGCTTAGAATGGCAAGTGCCAGCAGCGAAAAGGGCCAGGTGGCGAC GCCTCCCGTGGCTATACCTTACTCACCAGTGGTGGCCCATTGGTTCTACAAGCGCAGCGTGGACACAAAGTTCATCTGGA CACCATTCAGCCACTATGATTCGGCCTTGTTAGAAACCAGCCTAAATCTTGACGATTCCACATTGATTATTCCCGTTGAG GGTGGCCGCTACGATGTAAACATCAAGGAACGTACCAAGACTCCCGTTTACTGGGAGGGAAAGGCCATCGAAGTGCGACG CTGCTCCTGGTTCTACAAGGGAGTTGACTCCAAGTATGTTCCATACACCGAGGATACGGCTGCACTACTTGAAGCGGAAT ACAAGCGATCTGCGGAGACTGGTGAATGGCATCAGAAGATTATGCTGGCCAATGGCGAACAGGTGGTCTTCCACGGACCT ACAGTCATCGTTCACTTCCTGCCGCAACAGAATGCAGACACTTGGGGAGCCAGCACACAGGGTTCCATGCGTCCGAGAGT TGTCAAGAGGGATCTGGATGATTTCACCATCGAGCAAGGCGAATCGCAGCGAGTCGATCACCTGCTTTTCATGGTCCATG GCATTGGCTCAGCCTGCGATTTGAAAATGCGTTCCGTGGAGGAAGTGGTGGATGACTTCCGGGTTATTGCCCAGCAACTA GTTCAGTCGCACTACAAAAACTCAACAGATATGGGCCTGGTGGGTCGCGTTGAAGTTTTGCCCATCTCGTGGCACGGACA CCTGCACTCCGAGGAGTTAGGCATCGACGAAAAGCTGAAATCCATCACTTTGGAGTCTATTCCTCGCTTGCGAAACTTCA CCAATGACACGCTGCTCGACGTGCTGTTCTACACCAGTCCGAAATACTGTCAAAAGATCATGAACACGGTGGCAGATGCG CTTAACGATGTCTACCTGAAGTACCGAATGCGGCACCCTGAATTCAATGGGGGAGTTTCGCTGGCAGGTCACAGTCTCGG CTCCCTAATCCTATTCGATTTATTGTGCCATCAGGAACCGCTCAAGGAAAGCGAGGAAGAGAATAAAGAGAATCCCGACC AACTTCCCCAAAAGCAATCGAGTCAAAAGGTACAGCTTCCAACCAGCGATCCTTTGCCGAAACAAGTCAGCTATGCGATG GGTCCGGAGGGCACGGGACAGCCCGTGATTACATATACACAGCTGATATTCCACCCCAAAAAATTCTTTGCTCTGGGCTC ACCCATCGGAATGTTTGTAACCATCAGGGGGATTGATAAGCTTGGTCTCGACTTTCACCTGCCCACGTGTCCGGGTTTCT ACAACATATTCCATCCCTTCGATCCGGTAGCCTATCGGATCGAGGCGCTTGTCAATCCGGACATGAACGGCATTCGACCG GTGTTAATTCCCCATCACAAGGGACGAAAACGAATGCACCTGGAGCTGAAGGAGACGATGACACGTGTGGGTGCGGACAT CAAGCAGCGATTTATGGACACATTCAAGACCACGCTGGACAGCGTAAACTTTTTGACCACGGTAACGAAGGTTAAGAAGG AAGCGGAGGAGTCGCTGGAGAAGGAGACAAGCCAGACTTCATCGCAAGCTTTTCAAAAGCAAACCGAGGATCAGGACGAG AGTTCAGTTGCCAGTTCATCTTGCAAACTACGAAATCGCACCGATTCCAATTCGACGACGGCTTCTGACCCTGAGTTTAT AGAACTTGATTTCCCACTGGGTAAGCTGAACGACTCAAAACGTGTGGACTACGTGCTTCAGGAAGCTCCACTAGAATTCA TCAACGAATATATTTTCGCCCTGAGCAGCCATGTGTGCTACTGGGGCTCCGAAGATACGATCCTTTTCGTGATGAAGGAG ATTTATGCCAGCCTGGGAATCAGCACAGACAGCCAGGTGCCGCAGTCCTCAATGACCATCGAGCGACCCGTTTCGCATGA GAGCAGTGTTAGCCACTCGCTGTTGCCGATGTGAGTCCACAAAACCACACGAGAGCTCTGTTGGATCCAACGATCCCACG TACCGTATACACCATAACTATATATAAATTTAGTACCAATTTATTTAGATATGTTATTTGTTAGCCGAATCATGCAAAGA ATTTTCCATATGTGCAACTCCAACATGGTAAAAGGTATTATCAAAATAAGAAGTCTTTATCGTTTGAGTGACAAAACCGT AGCAAGTTCTTGAGCTTTACAAAATTACCTATGTATTGATCGGATTGGCACCCATGAGACCTATTTGTTATTTTTGTAAT ATCGCCTGTGTAACAACTAACTTTGTAACATTTATGTTAAACGAAGTGTATGTCTAACAAATTTTAAAATAAATAACTAC CATTGTTTTCAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
