Comment: 0-22 hr AEL
CAGAATTTGTGGCATTTTGTGTGGCGTCTTATTATCGGATATTTAGATTTTAACTGTAATAAGAACAGGGATAACACGAT GCTGAGCCGCAAGCGCCGGGCGAGCAGCATATCCAGCCGGCAGGACGAGGATCCGCTGCAGCTGGACGACTCGACGCCGG AGCAGTCACCGGTGCAGCAGACGACGACACAATCGGCGCGAAAAAAGCGCCGTCTCGATCCCACAGAACTGTGCCAGCAA TTGTACGATTCCATAAGGAACATAAAGAAGGAGGACGGTTCAATGCTGTGCGACACCTTCATCCGCGTGCCGAAGCGCCG GCAAGAGCCCTCGTACTATGACGTGGTGGTCAATCCCATTGATCTGCTCAAGGTTCAGCAGAAGCTGAAAACCGATTCCT ACGACGATTTGGATGATCTGATGGCCGACCTGGAGCTGCTTATTGGCAATGCCAAGGCCTTCTACATACCGGGCAGTAGC GAGCACCAGGACGCAGTCTCTCTGTGGCAGCACATTCATTCGCAGAGACAGCGCATTATGGAGGCAAATGGATTGGCGGA GGAGGAGCCGCGTGCCAGACGCATGTCGCGCCAAGTGCGCCGAATGACCAGCAGCACTGAACCTGGTGGCGATGGAGCAA CTGATGACGAGTACAACCAGTACGAGGAGCTCTTCGCCTCCGTTATGACCGCTACCGATCCCGTGGGCGATCGGTTAATG CACCGCATGTTCCAACTGCTGCCCTCCAAGAAAATCTATCCAGACTACTACGATGTCATTGAGCATCCCATTGATCTGCG CCTGATTGCGACCAAAATACAAATGAACGCTTACTCCTCGCTGGCGGAGATGGAACGGGATCTGCTACAAATGACCAAAA ACGCATGTCTGTTCAACGAGCCTGGTTCTCAGATCTATAAGGACGCCAAATCCCTGAAACGGATCTTTACGCAGCGGCGT ATTGAGCTGGAGATGGGCAAAGGCAAGCTGGCTAAGCGCGTCAAGAGTCTCTCCAGTGCAGCAATTGCTGCACTAAAAGA GGAGGTTGACAGCTCGGACGACGAGGAGACCAGCAAAAAGGGCGAGGGGCCCATGTGGGCATTGTTCGATCACTTGTACA ACGCACCAGGAACTTCGGAACATCCGGGAGTTACTGGTCCTCCATTGGGAAACTCTCTATGGAAACTGCCAGTTCGTCGT TTCCATCCCGAATATTTTGAGCTGATCAAACGACCCATATCTATGAGCCAAATCCACACAAAGCTCAAAAAGGGCGATTA TGCGAACATTAGTGATCTCACCGCCGATCTATATCTCATGCTGGACAACGCCAAAAAGGCGTTTCCGACGTCCCATCGCA CTCACAAGGATGCGCTGAAAATGTTAAAGCTAATGAATGCCAAGCTGGTGGAGGAGTCTCTCGAGGAGGGCAGTGATCTG GATGACGAGGATGCAGAGGAAATGGACACAGAGGTATTTACGGTTAGCACGCAGCCGGAAAAGCGCAAGCCGGGCAGACC CCGCATCAACTCCAATTCGAACTCTAACGCCTCCCATACACCAAATAACTCGAATTCACCCAAATCAAATCGCATTGCCA TCAACGCGGCTATTAAAAAGAAGATTCTGAGCATACAGAAATATTTGGTGGACTACTCCTTGGGCAATCGTCGTCCCATC GAAATGTTTATGGAAAAGCCGCCTCGCAAGATTTATCCGGACTACTACGATATCATTCAAAACCCCATTGACATGAACAC TATCGAGCATAACATTCGCACAGATCGCTATGCCGCCGTGGAGGACGTGGTATCCGACTATCGACTTATGTTTTCCAACT GCCGACAGTATAATGAGGAGGGCTCTAACATATACGAGGATGCCAATATATTGGAACGAGCTCTAAATGAAAAGCTCAAG GAGTTCCCTGGTCTTACAGAGGGCAAAAAAAGCCAGCAGAAGTACAGCAAGGTGGGAAGAAAGTTAAAGACCGCTGTAAT TACAGAGCGGCTGTGGCAATTCTATGAAACAGTAAAGGAGTACCAGGAGCCCAAGGGTAAGAGGCAGCTGTCGCTTATAT TTACGAAGTTGCCATCCAAGAGCGAGTATCCGGACTACTACGACATTATCAGGGAACCTATTGACATGGACCGAATCGCT CAGAAACTTAAACAGGGCGCTTATGATACCTTAGATGACTTGGCAGCGGACTTTCTGCTGATGCTGGAGAACGCCTGCAA GTATAACGAGCCAGACTCGCAGATCTACAAGGATGCGCTCGTGCTACAACAATTGACACTGCAGCTGAAGCAACAACTGC GCACCGAGCGCGATTCTCTTCCGGATGTTCCTTTGGCTGTTCAGGAGCTGTTCCTCACACTGTTCACGACTATTTACAAT CACCAGGACGAGGAGGGACGTTGCTACTCGGATTCCCTGGCCGAACTGCCGGAGTACGATGAGATTGGCGAAGGACCCAA GGTTCGCGGCATTTCGCTGGACCTTGTAAAACGAAGGCTTGACAAAGGAGCTTACAAACGGCTTGATGTTTACCAGGAAG ATATTTTTGCCTGTTTGGAACGTGCAAGAAAGTTGTCCCGCACAGATTCGGATATTTTTCAGGACTCCATCGAGTTGCAA ACATACTTCATTCGCAAGAGAGACGAGTTATGCAAGGACACACTTAGCTCATCCGCTTTAAATTTTAGTCTAGAACGCCT TATGGCTGATGTGGAGCTGTGTCGCCAGCAAAAAATGCAACAAGAGGAACAGGACCAAGAGCAGGATAAGGAGAAGGATG AATTCAATGCAGCCAAGGGTGAAAGCATGACAATAAACCAGCAGGTGTTTTCTCCCGGCGATTACGTATACGTGCAAATG CCGGAAAACAAGATTCCCTCGATATGCTGCATAGAGCGCCTCTGGACATCACCCACCAATGAGAAGCTGATGCAGGCTTC GATCTTTGTACGGCCCCACGAAACGTACCATGTAACCACTCGCAAGTTCCTCGAAAAGGAAGTGTTCAAGAGCAGCCTTT CGCAGACCATCTCGATGGATAAAGTGCAGGGCATGTGCTACGTAATGAACATAAAGGATTACATCAAGATGCGTCCGGAA AACTTGCCTGAAAAGGATGTTTACGTTTGCGAGTCTCGTTACAATATCCAAGGTCGCTGGTTCAAGAAGCTGAAGAGCTG GCCGCCAGTCCGTGAAGGCAGCTCGGTGAAGTTCGTTCCACGAGAGCAGCCATTGGAGTTAAAACGTGTGATGTCTGTGT TTAAGGAGCGTTTGGAAAAGCACAAAGGAGAACTGGAGGAGCTGAAGCTCCAAGAGACGCTGGTCGAAAAGGAGAAACCA AATGTGTCTTGCGATCCGCCGCCAAATGCTGAGGTTGGCAGCACGTACTACCAGCAGTACAACACCATTTGCAGTGGGGC CATAAAAACGGGAGACTTTGTTTACGTGGCCACCCAAACAGGAAAGCAGTCGGTGGCACAGGTCCAGCAGATTTGGGAGC AGAATGGAAAATCATACTTTAAAGGTTCATGGCTGTTACCACCCAGCGAGACGACCCCCGGCTTGGGCAAGCAATTTTAT CGCCAGGAACTGCTGCTGAGCACAGTCGAAGAGGTCAGTCCGGTGGTGGGCATCGTGGGTCGTTGCGCCGTCCTGGAGTA CTCCGAATTTATCAGCTCCCGACCCACGGAGATATCCGAAAGCGATGTGTACATTTGTGAGTCCGTCTATGATGAGCTAA AGAAAGCTCTTCGCAAGTTGGTTACGGGCAACATGCGCAAGTTCCAACACAGTCCTTCTGTCACCGAGGATGAGATCTTT TACTTTAAGACTCCCATTAAGCCCTCCAAGGACTTTAAGAACGAATTGAATGAACTGGGCATGCTAGAGGATTCAATGGA TGGGGATACACCCTCGCTGAGCTCAGACATTGCGGCCATGTCCTCGCCGGCTCCTTCAGTAAACTCCACGCCACTGACGT CCAAAGTTAAGGCCGCCAAATCGGCCAAAAAATGTCTTACCGGTTACATTTTGTACTCTAGTGAAGTTCGAAAAAGCATT TGCCAGAGCAACCCGGACGCAACCTTTGGAGACATATCCCGCATGGTGGGCACCGAATGGAAGAATCTGCCCTCCAGCGT TAAGCAGAGCTGGGAGGATAGGGCTAGCCGACAGAATGAGGAGACGGCGGCCCTACGGCGCGAGTTGGACGACGTCCAGA ACAGTGCGAGTCCCTCATCCCAAGTTTCGCAGGATGTCTTTGGACAGGTGCTCACCTTTGAGTGTCAGTGGGATAAGTGC GACTTTCAGTTCGAGGAGCTTAGCGACTGCACGGAACATTGCATGTCCGATAGCACCGGACACATCCAGCGCCATCCACA AGCGGGCGTGGAGACGGAGTATGTCTGCCTGTGGCGCAACTGCCCGCGGGTCAAGAAGTCTGTTCAGGCCTTTCCTAACG TTCTGCGCCTCATCAAGCATGTGCGCGAGGTCCATCTGAGCAAGTGTGGCAAGACGATTTCGGCGGCAGACCGCAGCAAG AATTTCGTGCCGCGTCGGCAGAAGCATGCGCAACTGGCCACTACTGTGCCCATCCCGCCCAGCCTTGCGCAATCGCCGCG CGCTCCAAGCAATCTCGAGGCAGTGCAGCTCCAGCAGCAGCAGCATCCGCAGCAGAACATGCACCAACTGCAGCAACAGC AAACGATCGTCTTGGGTCCGCCACCAGAGCCGCTCTTCGTCACAGTGCCGCCGCGCACCACTCGCGTCATCCACTCGGAG GCCTACATTAAGTACATCGAGAGCCTGCAGACGGGCAGCCATCTGAACGTGGTCACCTGCAACAACAATTGGCGGCGTGC ACTGACCCACATCACACCCGCACAGGTGGTGGCCAAGACGGCGCTACCCGAGAAGTGGATCGGACCGAATCTGCGCGACC AGGGCAACGTGGTGCAGGCACTGTGCCACCTGCGCAACTTCATGGTCGACGATATACTTCAAATACGGCGCAGTTGCAAC TAGCCCATCGTTCACCGCTAAGCCCAAAGCTTTTTGAGTTCCATGTTAATAAACAACACAAACAAATTATAAAAAAAAAA AAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
