Comment: 0-22 hr AEL
CCGTTTTAAACACAAACAATTTCGCAACGCAGTGAATCTCTTGTTTTTCCGAGTTTTTCCGCGATCGCGAAGTGCGCGTT AAAGTTAATCGCTGTCTGAAATCCCGTTAAGCTGGAGAAGTGGAAGAGAACGCGCAATTCCACCAAGAAGATGGACGGCC GAGGGGTTGGCGGCGGTGGTAGTGGTGCTGCCACAGGGGGAGGATCCGCCAACAACAAATCAGCTGCTGGAAATAGCGCC GCCACTGGAAATGCCGATGCATCTACCGCCACAGCGGCGCCCAATGATAAGCCAACGATTCTGGAAGGCGTTCTCCACAA CATCTTCGATGGGCTGATGGACAAGGAGGAGCAGGTGCGGATTGCCATGCAGCAGGCGATCGTGAAGATCTTGGAGACCC ATCCGGAACGGGCTGCGGAGATCCTCAGCGAACATCGATCGCAGCAGCCGAAGATGACCGATCAAACAGTGGCCATGTTG TTGGACTGCATTCGTCGCGTGGTGGGAGCGGAAACTCCTCTGCCGCCGGCTGCAAATCAGAAACTTATTGATCTTGCGCT GCAGGAGCTCACCAAGAATCCCGAGCATTTGCCACTCATACAGAATCCCGCCCAGCGCATCCTGGTAGCCATCGGACGGA GCAGCCCAGAGAGTTGTGAGCAGGTAATGGAGGCGCTCCAGGAGAAGGGAACAAGTACAGAAGGTGTGGCTCACTTTATG CTGATGCAGTGCCTCGGTCTGCTGGCCACTGAGAATCCGGCAGGGGTTGTGCCCCACATAAAGGCCATATTGAGCCGTAG TCAGCCGCATCTAGTCGGGATACGCCAGGATCACATCAAGCAGGCACATGCCTATGCCATCGGTAGATTCAGTGAGGCTC TACTGGAGGAGAGCGGCAAAAAGGAGGAGGAGAACTGCTCCACGGAGATCAGTGTGGCCTACGATGTGCTCTTCAATCAA TGGCTGCACTCCCGGGAGCCAAAGGTTTGCGTGGAGATACTGCAGGCCCTGTCCAGCATGTATCCACTGCTGCCCAAAGA CCGGATTCAGGATCAGGCCGCCCGATTGGTTCCCCAGATCCTGGCGCTCTATCGACGCAGCGTGGACAGGAATGCCGTGA CGCAGTTCCTCTGCTCCGTGTTGAAAACGAATCTGGCACTAAATGCCACAGTCTTGGATGGCATAATTGACGTGTTGGTC ACCCATTTGTTCGACCTGGTCTGCGTTTATCCGGACTATGAGAAACCGCAGACGGTGAAGGGTCACTACGAGGTGCTGCG TTGCTTCCACCTTCTGGCCGGTCATCAACCCTACTCCACGCGCATTATGGACACCCTGCTGATCCACCTGCGGAACAATA GCGAACGCGAGAGGATGAAGTCCCTGCTGATACTCACGCACCTACTCAACTCCTGTGCAGGGAATATAGAAAACCGGATT CCAGCCACCATTGAGTGCCTAAAGCAACTGATCCTCAGCGAAAGAGGGATCAAAATGAAGCTAACGCTGCTAAAAACCAT TGTGGCTCTCGCCCAAAAGTCCCACATCCGGGACAAGGAGTTTGTGTGGTTCGTGGTGCGGCATAGTTGCCGATTCTCAA AGCCAAGTCAGGAGCACGGCAGCCAGGAGGAGCACGCCAATTTGGTGCTGAGCTGTGAGAACACCCTGTACATGCTGGCC TCTACGGTGGGCACCTTGGATGAGCTGCTCAAGAGGGAGCTGCTGAACTACTTGATCCTACTGGACTACACGGATATTTG CGGGAATCTCGCCAAGTGCCTGGCTAGTTTGTTTGCCAAATCTCCGCACATAGAGTACGATATTGCGGGAGACGATGCCG CCACGGAGCAAGCCACACCGGAGAGTATGGGCGCTGCTGGCGAGGATCGCAGTGTGATCAAGCGCGGAAAGGTTATGGTC CCTGGAGCCGAGACGATTTACGCAAGATGTCTGGCACTCCTGGGCAATCAGCAGTGCATTAAACGATGCTCGAATATTCT CAGTTTTCTGCGCTACTACCATCCTCAGGTGAATCCCGCCCTGGAGGAGCTGTGGGAGCGAAAAATTCCCGATCTTCTGC TGCAAATCAACAGGGAGAGTGCCTATCGCCAGCTACTCCACGATTTTGTGCTTGAAACGAATGAATTTCTGGGCGGATTG GATGAGAACTTTGCCCAGCGGCTGGCCAGTAAGCTGGCGGACCAGATGTACTTGTATCCCATGCAACTGCCCCACTCCGA GTGGCATTTGCCTGACCTTAGCGCCGAGCGAGGCATGCTCCTGCAGGCGGTGGCCCTCACCCTGCTTCAGGTGACCGATG TGGCCTGCATCCACACCAAGATCGATCTCATTGTGACGACTGCCAGGCAGGAAAGATTGGACAAGCACGTTAAGCATGCG GACTACGAAAAGCGCATTGAACCCTGTGCCAGAGCCTTGGGCTACATTTCCCGCCAACACTTGGGGCATCTGATCAAAAA GCTAACCGAACTGGCCCAAGTCGGTGGGCGAAAGCACTCCACCGGTTTCTTTAGCAATCTGCACTTTATCAAGGATACGC ACAAGGAGCTAGAGAACTACAAGAGCAACCTGCTCGTGGTCAAGGCTTTTGGTCGCATCATGGACGAAGCCGATCCGTTG CAATCCATTCAACACCTGGACGAAGATGACACATTGCTGGGCTTCCTCATCCAACAGCTGGCCGTGCACAAGGACCAAAC AATTATGTCGGCGATCCTGCAGACGCTGCTCAGCATCTGCAACCAGTTGATAGCCACCAAGGAGCAGCTGCCGGCTCCTC TGCGTCATCGCAAGCAGATCATGGAGACGGTCTTCAACATCCCCATCGAGGCGCCGTTCCACGACCTTCCCCTTCTGCCC ACCATTCTCAAACTGGGCACGGACTTCATACGCATAGGTGGACCCGATACCGAGGAGTGTGTGGACGGCGGGGTGATCTT CGAGATCGCGTGCCGCAACTTCTTCGGCTGTGCCCAGCAGCTCAAGATGAAGTTCGACTCTCAGGAGGAGGATGAGCGCA ACAGCTTCCTGGCCAAGCACCTCAACGAATCGCTGCCGCAGTTGAATGCGCTTGTGAGGGCCATCGTTGAACTGGACGCC TCGCCGGCCACACTGGATCTGATTATTGGCATTCTAGAGGGCTGGACAAGGGATCGCAACTCTGAGGTGCGAATCTGTGC CAGCCATGTGTTTAACAACACCCTCGATGTCTACATCAAGTCCATGAAAATTGGCTGCGAGGCCCCGTCGAAGTTCAATC AGACTGGTCAAATGCTGGGCAAGATTGTGCCCAGGTGCATCGATTCGAATGGCACGGTGCGGCAGGTGTCCGTGGAGATC CTGCAGAAGACGCTGGAGATCGCGTGTATCTACGAAACCCTGACGATAGCCAGCATCGACAGCACTGCCGACTGGCTGAA GGAGATCGAGACCATCAAGGAGCACATAATCACCGACGAGCCCAAGCAAATCTACAATCTGGCCGGCGACATTGCCAAGA TTATTGCACTGCGTATCTCCAGCTTTCAGTACCTGCAGTTCTGCAAAACTCTACTTCATTCGCTGCGTGATCCTGAGCAG AGTTCCACCATTGGCGCCAGTGTTGTGCTCAAGTTCTTTATCCAGCAGAAAGGCTCTGAGCTGTTCCACGCCATTCCCGA CCTGGTTCGCGACAGTTTGCTGGCCTTGCGCGTGTGCGAGGTGCCCAGGGCGAAGTCCGGAGTGCTAAAAGCTTTGGTGG CCCTCACCAAGCACCATCCCAAGTTGGTGTGTGCCGAAATGCTGTCGCAGCCGCTGCCGTACGACCCCAATCTGGTGGAG TACTGGCACCTGGTGTGCAACGACCCGGAGTTGACGGGCCTCACGCTGGACAACTTTCTGCAGCTGCTAAGTGGTGCCTC CCTCCAGGAAGGCGGTCAGGATGCATCTCTTTCCGAACGTCAGAAATTAGCCAGCGGCCAGCCGTTTGCCATTTTCTGCG CTCTGCATGAGATGCTGCCGTGCAAGGATATCAAGGGGCAACTGGAAACAAGGTTTGCGGACATGTTCTGCATGCTGCTC ACTTCGCTAAGCAGCTACACCAACTTAGCTGCACCGAATCCTATAAACAATGCCTCTCTAAGTCCCAACCAGACGCAATC GGGCAAAACCAAATTCGGTTTCGTGCCCAACAAGGAGCTGATCAAGCTCAATCCCTGCCAAATAGCACTGGAAACTTTCC AGGCCTTCCTGACCAATCTAGAAATGGAGCAGATTGCCAGCGTGCTGACTGTGAATACGCAACTGGCGAGCAGCGCGGAC TGGCACAACTACATCGAATTGCTGACACCGATGGCCATCGGTCTAGGTCAACAGCTGCAGCTGGGAAGTCCGCAGATGAG GCAACTGGTCAACTCGCTGAGCAAATATGTGGCTTCACCATACGATGGTCAAAGAGTGGCCGCCGTGGGTATTTTCTCAC GATTGGTGCCACTGAAACCCACCGGTGAGCTAGCTGCGTCCATACTGCTCCACCTCGGAGCCGCACTATCCGATCCCAAT GCGGTAGTGCGAGGTCTCAGCATCCAGGGCATGGGTTACGTGGGCCAGTTGGGAGAAAAGGAGGCGAAGCGCTACTCGGA AACTGCCATCGGTGCTCTGTTGAAGGGAGTCGACGATCCGGTGGGCGATTGCCTAATCAACATTCCGCTGGAGAGCATGC GCGGCCTGTCTGGAATACTGCGAGCATTGCCCAGCGAGCGGGTAGAGCCCTTCCACGTCTCCCTCGCCATCCGGATTCGA CCGTTTCTCGGCAACTATGCACTAGAGATGCGAGAGGCGGCTATCCAACTGTTTGGCGATATATGCGAGGGCAAGCACGA TGACGGCAGCAGTTCACCCACATCCTCGATGGAGGCACTGAGAGAGCAGCTCATCGCCAATCTCTTCCCGCTGCTACTGC ATCTCAGCGAGAGCGAAGTGGCTATTGCCTCGGCCTGTCGTGGAACACTTCAGCGGGTTTGTCGTCTCCTGACAGCTCCA AGGGTCGTGGAGATGGCGGAGCAGCAGTTGGGCGAGGAACGAGGTCACCAGCTCAACTACAGCAGCTTTGTGCTGGAATT TGTCAAGACAATTGCACTGGAGCTGACGGATCACATCCAGGACTTCATCGATTCGTGCCTGCCGCAGTTGCGTAGTCAGT GGCCCGAGGTGCGAGGTAGTGCAGCCATTGTAATTGGCATTCTGCACAACTTCCTCAGCGAGAGAAACGTCCAAACGGAG ACTGTGGCCAGCAAGATTGCCGTGCTGCTCAAGGACGAACAGGCGCTGGTGCGGATGAGGGCAGCCACTGCTCTGGGTTA CTTCTTTGGGGACATCTAGAGCATCCAAGGGACCAACTCGATTAATTAGATGTGCAGTTATGTTGTGTGTGTTTGTAAAT TATGACTTAGTATTGCTCCAAGTGTGATCCCTTTGCCTCAGCAATCGTTCCCTTATTATGTTTGATATTTAACTCTTTGT TTTGTTATGTAATCGAAAGTCACGTTATATACTATCTGTATACACATAGCATTATTTATATATTTATATTTCCATGTTAA TGCGGAGCGTTTTGCAGCTTTAAGACAGTTGGAAGTTATAGGAACTCGTATTCGACCGAAGACTTTGTTACGGACAATAA TTACTTCATATTATACTGAGTCTATTCAAAATTACCTTGGCTTTTTGAGGTTCATAAAAATTAAAGAAAAAAATAACTAC TATGATTCCACAGCAAAAATACGTTGTCAAAAATTGATTGTATTCCATTAAGGAAGTGGTATTTTATTAAAAATATTAAT AAATCCTTATAAGCGTAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
