Comment: 0-22 hr AEL
AGATAATCTGCGAGCATGTCAAGATCACAAGCTTGGTGGCCGGTGCCGTGCAGAAGCTGCCGCTGGATATGCGTATGCAG CTGCAGCAGATGAAGCTGTCCGAACGGGTCAGCGAACTGATAGCCGCCGGCGAGAGGAGTAGCTGTACCAATGAAAGCAG CACCCCAGGCGAGCCCACCATATCACAGTCGCATCGCAAGAGAAGGCGCTTGCGCACCCATTCTCCCGTGAACAGCATTT CTTCGCCTTCGTTTAGCAAGCGACCGCGTCTGCTGCCGCCACACTTCTACTGCAGCGTTAACCGCGACAAGGTCAGGCAG AGTTTCCTAGGCAGCCAGGATGACTGCGAGGCGGAACTGGACGAAGAGGATGGCGAGAGCACAACGGCCACCGAGGACTT GGAGGAGGATGACTTACTTCCGGCGAATGGTCCTGGTCCGCGCAACAATTCCACGCCGCGCCAAGGTCATACCGAGTCCA ATCCACTTCTGCTCACGCCGCAAAGCATGCCTGAACTGGCCTGCGCGATAATTAAGAATCAGGACTTCCAGGAGACGCTG GTAAAGAATATTAACGTAGCGCTGCAGACGGTGACTGTTAACAATCCGTCGGTAAGCTGTGACGCCATGTTGGATGGTCT AGTGAAGAACATACTGGAGGCCACCGAGAAGGATCCCTCGTTCGATCGCATCATACAGGAGGTGGTGATCGGGGATGAAC CATTGGCAAATGAGGAGGACCCTGGTGTGTCGCCTGCCTCTGATGCTGCTGGGGCTGCTATTGTTCCCGCTGCTGATGGA GATGTGCTGCCGACAGAACAGGCCGTGGATCCCGTGCCGCCGCAAACACCGCTTATTATTCGCACCGCCGTTGCAGCCAC CGCAGGAACCAATGCAGATCCCAATTTCTCCATCTCCAAGCTTATCGTACTGAACTCCAACGAGAGTGTCCAAAAAATGG TTGCTGGTTTGGATACGTCCAATATCAGCTTTACAAGCGAAGGCCTCAATATAAATTTTGCCGATCCAGCGTCAATGCTC AGCGAGGTGGAGGCGACAGCTGCTGGTCAGGTTTGTGTGGACGCCACCACCGGGCAGCTGACGTTTCCCATGTACCTCTC GAACGGTGGACTGCTGTCGCATCTTCCATTCCTGGTGAACAACGAGTGGGTAGCCCAGCAACTTGGGCCGGATGCCTTTG CCAACATTGATGACTCGCACATTGAGATATCGCTGCCGGAGCCGATTACTCTGTCTGCCAATCAGCTGCCCCCCAATTCC ATTATCATTAGCAGCGCCCAGAAACAACCACCTCCCCCAAGCGTGGAGCCTCCAGTCCAGTTGATAAAAGATGCAGCCAA AGTGGACAACAAGGCGGGCCAGGGAACGGCGGCAAAAGAGCAACCGATAGATCAACTAGAGGAGGAGCAGCGAAAGTTTC CACCCGCATCCCTGGATTCATCTAGACAAGTCATGGAGAGAGTGACACCCTCGACGTCGGGAATAATTAACGTAAAGGCC TTTCGTAGCTTATCCACTCCCCGAAAGAGAACTTCGCATGTACGAACATTAACTTTTTCCCCTAAGGTTCCTCCTGGTGG CGTCCTGCATTCCAATACGCCGCTGTCCACTAGGCGACCTGGTTTGCTGGCCAAAGCCCAAGAGAAGCCAATTATCAACC ATGTGGAGATCATCCAGGCCGCCTGCGACCTGAGCTCAAACGAGGGCCTTGGAGGAGTGCCACCACTCTTCGCCATGGAG GAGTGCAGCAATCAGACGGTGATCAGGGCCCATCCTCCAGCTGCCGCTCCGTCAGCAGCTCCGGTTCCTCCGTTGGTGGC CACGCCCAAAAGAAAACAGAAGCGACAAGCAGCGGTCAAGGCCTGCAAGCGAATCATCTCCCAGGCGGAATCGGAAACCA AGACGGATAAGGATCAAAAGAAAATTATGCCCAAAAATAACTCTGCCTGCAGCGCATCGTCGACCGCTCACGATGACAGC GCCGAGGCCAGCAAAGAGAATCTCCAGGAGCAGCAGAAGATTGAAGGAAAGCCAGATGTAGGCTCCGCCGCCAGAGGAGA CGCAGTTGTAGATAACGATATGGCCGCCTGGCAGCGCCAGTTGCATGGCTCCAATTCTGACCTTGAAAATCGGCTGCGTG AGATTAACTCCAAGCGCGAGGAGGTAAAGGCCTCCGGCCGCTCGAGGCGTCCAAAGAAGAAAGATACACCCACTTCAGCT TCGTCGAGAGCACGAAAGGCTCCAATGGTCAAAGCGAAGGCGGAGCCCATGAATCTGGCAAAGAAACCGCAGCGCAGTGT TGGGGATTCCACTGAGAAGATCAACATCAAGATTATCACTCCTCAGAAACCAAAGCTCCTGAAGAAAAAGAAGGTGTTTG AGGCAAAAGAAGAAATCATAGACGAAACCATAGAAGAAACCATAGACGAAACCCAAGCGGAGATCGAGGAAAAGGATCAG AAGAAGAAAAGTGAACAAGGATGCATATATGCAAAGGATCAGGAGCAAGCGAAGGCCTTAGCGGCAGAACTGTCGCGACC CAACATGGCCATGCTTCTGGACACTCCGTTTAAAGCATCTCCCATTGGAGCAGGAGCAAGCGGAGCCGTAGAAGATGACA CCACATCAAAGGAAGCTGGTGTTGGTGTAGCCGCTATCATCCCACCCACGCCAGGAATGGCCATTCCACCTCTGGACACG CCATATGGCAAGTTGCCCAGTAGCAGCTTTCTCTTTGGTTCGGATACCAAAAGTATTATGGATACGCCCCAGTTGAGTGC CATCACACCTGGATTTCGCTTGACACCTTTTGGACAGATGCCCGGTACTCCGATAGGCAGTGCTGCCAAGACTGAGTACT CTTCAGGCAGTTCCTATTATCGTCCGGATGAAGCGGAACACACCGATGCGAATGCGCAGTGCGCAATACAACAGTGGGGT GATCCGCAGGATAAGAAACAACCCAAGGTTCGGTCAACGCCCAAGAAGAAAAAGGTTGAGGCAGAGGATGAGCTTTCGCA ACAGAAGAATCAAGAGCTAATAGAAATGGAGTGTTCAAAGAAAAGCCTTCATCCGGAGGACAAACATGCTGAGGACAATA AGAAAACACCGAAAAGACCAGAGAAGCCAATGGATGAACATCTGAAGGAAACTGAAAACGATGGTGAAAAGAATGAAACG GTTTTAGAAAAGACACCAGATGCTGTGAAGCAACCCGTTGTCCTGTCCTTGGAGCCCACCGTCCTTCGACGCGTTCGATC CTTTGGCAGTGAGGCGGTGGACAGTGCGGAGTCCGCTACAGCTGGCTCATTTCCCTCCGATCAGCAGCAGCTTCCGCACT ATAAACTTTTGCCTGGCCTGCCCGAGGCAATTATTGAAGGATCTAGTAATTCTAGCTCCTCGGCAACCAGTTCATCGTCC TCATCCAGTTCGAGCTCAAGCTCCTCCAGTAGTTCATCATCGAGTTCCTCATCCTCGTCAAGTTCTCACAGCGGAAAGGG GGAAAGCAGAGGGGAAGAGGCAAATGGCGAGAATGCCGACGAAAAGTGCAGCAAGAAAAAGGAGATGCTACTAAACATAG ATAACTTATCGAACATTAGCAGCACGGAAGACGAAGAGTGGCTGAAGGCAGCCGGTTCTGAGGATGCAGTTCTGCCCATG CTTGCTATCGACAGTCAGCCAGGTCAGTTAGTTAGCCAGGACGGGGAGGTCCGCTATCCTATCCGGAATTGGCTGACACC AAGCAAAGAGCAGGCCCAGGAGACGGATACCAGTGGAGAACAGCAAACAAAACCTACACCTCCTAAACCGGTGGCTGCTC CTGTTCCGCCTCCGCCGCTTCCTCTAACACCGCCAGCTGCTCCTCCGCCGCCTCCACCGCCTCCGCCAGAGGCGGACGAT CCTTCATCCGTCGCTCTTCCAACGGCCTCTACAAGCCGTCAGGAACACCAACGCCAGCAGCTGAATGAAAAACGCCAACG TGTAGTGGCCAAGTTTAAGGAGCAACCCCAACCGATCAAAGGTCAAGGGAGAAAGACTGCCAACAAGAAGCTCACTGCAA TGCGTGAAAATGCCAAACTGGCCAATCCAAGCAGTCAAAAATCGCCAAAAAATCGAAAGCACATCGAACCACTGAAAACA AAAGCAGCTAAAAAGCAAATAGAACCAAATCCGAATGTTAAGTCCACAGAGGCTACTGGTCCGCCAGTCGGTGCCGCCAC AGATGCGCCAGCAGATCCGCCAGCCGGCAAATTGGATCCCGCTCTGCTCATGGCCCTCAATCTATCTGCGAAAAAACAGG AATCATTACCGATTGTAATCAAACCAAAGGTAAATCGAGCAGCGGTTGAAATTGCGGCACAGCCTGCCCCTGGAAAACGT GGACGCCCGAAAAAGGTACAGAACGGGGAGCCAGCCAATCTCTGTATGCGACGATCCTCGCGCCTAGTCGAAAATAAAAC TCCTGCTCCGGAAGATCTCTCCAAATCCACTGAAGGAGCAAAGACGAAGACCCAAATCAAGGCAAATACCAAGAAACCGG CCAAGAAACGTGTCGAGGCAAGGGTACCCTCCTCCACAACCGATGCTCCGGCACTGCCCATTGTAGCGGAGGCGGAATCC ACCCAAAAATCTCCAAAGCAAAAGCCGCAAATTCAGCAAACAATACAAGAACCTAAACAGGATCCACCGCCATCGAAATG CGATTCGGAGCCCGATGACAGCGACTTCGAGCAGGACATGTGCCTGAGCAGCAGCCAGGAGCGTTTTACCTATAATTTCA CATATGCGGACAACGGACCAAAGTCCAGCAATCCGATGATACGTCAGCCATCGAGCTATTTCAAAAGCTATCAGATGCGC TTGATGACCGATGACGAACAGCTGCATACGCTGCGCATAGCCAGTGCCCAGGTGCTCTGTCTGGGCCAACCGGTGGCCAG CGCCATTCGCAATATACCCAAGAAGCGGATACGCCGCCTGACCGGCAATAGTGGCGGGACAGCGGCAACAGGGGGAAGTG CAAGTGTAAGTGGAGGAAGTGGAACGGGAGCGGAAGTTGCTGCTGCTGGCGAACAGCCTTCGGCCACGTCTACGCCGCTG GTGGAGAAGCCCCCATCCAGGAGTGGGCCGCAAGATGAGGAAGATCTGGAGGTGGCCACCATCAATTCAGCTCCGATAGC AGCGAACAGCAGCAGCGGAATTGGCGGCGAGGAGACACCGGAGAACTCTGCGCAGCAGAAGGAGCTGCAGAGCGAGAACC ACATCGTGGAGTTCGAGGACATCGAGTCGATACTCTCACATCTGCACGGCACCTGATTCCAGCTCCATTGAAGTTGTTAT CGTTTACATACTACATCTAGAACCATTTGTTGGCATCTCTAAGTTTTTTTTTAAGGAGACAGTTTTGAAGTGAATTTAAT TACTATGTGCACCAAGGATCGTTATTATTAAAGTATTTATTTTTTTTCGCTGTAAGCTAAGACGGAGCAGCACCACCTGC TCACACAACATTCACATGAGAATCCCAGAAGAATCACTAATTTATGTTTCATTATGCACATTCGTAAATATAAAGAAATA TATATATAAATATATATATATATATATATATATATATATATAAATACACACATATAACGCTGTATATTAATAGTTGATCT AAGTGTTAATATCCGTTTTTTTTTTTTCGTATTACTAAGGCCCTGCCCGAACAAAAGATTATGATTTGATACTTTGTACT TTGTCGCATAAAGAGAATATAAGGAATTAACCAAGCCAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
