Comment: 0-22 hr AEL
TTTCTTGCAAATCGGTGGTGCGTGTTCTCGTGCAACAATGGAAAACCTGAATGCGACCAGCGGCAAATGTTAAATTGCAA TTAAAAGTGTGGATTTCGCGGCGGTAAAACGCCAAAATTCGTACCGAATCGCTGCGCGCGTGTGTGTGTGAGCAGAGCGT GAAGAAGAGGAACGAAGAATTGCGAAAGCAGTGTGTGCGGAAGAAGTGGAAGAAGCACAAAGACAAAAGGAAGGTGAAAA TATATCAAATATATATCAAATCTATCCGGCAAAAGTAAAACAAATATTGAAGAAAACGGGCGGCATGCTGGAAAGCGGTA AGGGCAAAGCCAAAAGCAAGAACCTCGAGCGCTTCGCCCGAATTCGGTATTCAATCCTTTTTCCCGCCTCTAATTTTGCA GTGCTTGTGCCCAGAATTTGCTCTTCATTGGTGGCCAAGAAGTCAAATCCTGATCAAACGCGACCAGTTTGTGGCGCCAA ACAGAATATTATTTAAAAATATCGCGGCGTGTGTAAAACCTCGGCGAAACGCGAACAAAAAAAACCACGACGGCGTGCGT AAGCGCACTCACACCTTTGCACACGCACAGACAGAGAGAGAAGGCAGGATAGAGAGTGAGAACGAAGCGAGTGAGCGAAC ATTATCCGCACCTACACATACAAACTAGCTCGCAGGAATTGCAACAGTGTGTGCTTTTTTTTTGGTTTTTTTTTTTACTT ACGTTTTTTGTTGTGTGTGCGGCAACGCTTCTTTTTCGCGTTTTTTTTGCATCTCTCTTTTGTGCCGTTGCTTCGCCGGC TAAAAAGGTGAGTCTGAATCTAAAGCGGCATAATGGAGGATGCACAGGAGCAAAGGGAATCCTCGCAAGCCGAATTGGCC AAAAAGCATCCGCTGACGCCGCTCAAGGAGCTGCCTGAAGAAAGCGAGGAGGAGGAGGAGGAAGAGGATGAATCCTCGGC GGGAGCCCTACGGGAGTCTGACAGCAACAACTCCATCATGGGGAAGATGAAGATGCGCGTGTCCAGCATTCTGCCCGCCT CCTTGAGCGGTTGGTTTTCGCCGTCGTCCAAAGATGGGAACGATGCCTTATCCTCCCCAGCTAATCTCCGCCAGTCACAG CCACGCCAGTCCAATGGACGCCTCACTACCAAGCGGAAACGAGGACGCCGTCGGATAATGCTAGCCGAAGTGGATGCCGA TGCAGCCGATGATCTTGATGACGGCTCCGATGCCAAAGGCCTCAACTATGAGGAGGTGGCCCTGGCCGACAACATAGCCG AACACGACCTGGCCGCCGAGGATGAACAGACGCGGCGGAGCGAGTACAATGTGTTCCTGCTGCGCAAGCGAGCCGGGGCT GTTGCAGCTGCCGGCGGTGACGAGGATGAGGCCGAGGAGGATGAACTGGAAGAGGACGACGAGGATGGCGATGAGGAGGA TGACGATGAAGAGCAGGAGAATCTCCAACAGTCCGCCGCCGTGCAGACCAAGCGTCGACGCCTGGAGCTGGAGACCCCCG TCAATTTGCCGAACATGCGTCGTCTGCCCCTTTTGTCATCCACGCCTGCTGCTCCGCTCGCGGCTGCCACCAGCTCCTCC AGCTCCCAGATGTACAAGGGCGTCAGCCACATTGCGCCACATCGGCGCAATCACCTCAATTTGTATGGTAGTCAACGGCA GCGCGAACCCGCGTACAACTTTTTCACGGGCAACGAAGCTGCCGAGGGAAGCACCGGGGATCTGCCACACAGCATCCGCC GTTCACTGAACATACCTTTCGGAGGCAGCAGCACCGCTACCAGCTACAACAACAGCCTATCCAGCCTTCCTAACCACAAG CGACCATCGCTCATAGGCAAGCAAACCCATCGACGCGATCTTACCATGGATGAGACGGGGACTGGCCCGGCCATGAGTTC GGAAGAACATCTCAATCATTTGCTGAGAATAAGCAGGACCAACAACAATACTAGCAACAATAATAATAATAATAATAACA ACAACAATGTCATTGAGACAAAAACAAGACGAAGTGAACTGTCTGCGGCCGCAGGAGGTTGCGGTGATTCGCAGTCCGAG AGCGATATGAACGAATACCACGACAATGGTGAAGGGCACGACGGACTTCGTCCGTCGCATTACAACAGCAACAGCAATTT AGAATTTTACGGAAATCTGCAGAGCTCCAAGTCAATATTTAACAGAAGCAACACCGCCGCACAGCAATCGCATCGGAATT CTACGTGGTCGCTGAATTCCCTTACCCAACGACGTCGCTTCAATGCGTCCATATACGGCAGTACGTCGGCACTGAGCGAC AGTCGTCTGCTTAGTGGCAGTGCCTCTAACTCAGGCTCTGCATCTGCCTCTAGCTCGCCCTTTTACCAGGGACGCACCAC CTTCGGGGGCAATTCCGGCAACAATCGCCTCTTCAGTCGCAGCAATCTTAGCTCATCCGCCGCATCCTCAATGTTGGGAC TCAATTCTGCCGGCAGCTCACCGGCGCATCAGCTGCATGCTTCCATGACTGGTGGTATTGGATACGGTATGAAAGCGGTA GATATGCGACCTTCAGACTCCGGCAGTCTTGCGGAGACATCTGTGGGCCAAGGTGGCAGTAAGAAACCCGGTACCGGGCT GTCGAACACCACGATGCGCATTCTCAACCTACTTGAAAGCTACTCCACGCCATTGATCGATGCCAAGCGAATGGGGAGCA GCATCAAGGAGCATCAAAGCAGTCGCCAACAGCGCCAGGGTACGCCAGCCACCCCGTATCTACGTTCCACGAGTGCCAGC CGCAATGTTTCGGTGCCCAATCACATCAACGAGTTGGCGGAACTGCGCTCTAACAAACTGCTGGTGCCGACGATGCAGCA GCTTCTGGAACGTCGTCGTCTACACCGCGTTACACAGAACTCTCGAGATGTGGTTCACAGCCAGAACGTTCGTGCTGGTG GGGAAAACAACCAGGAGAAGCCCAAGCCAACTGCACCATATGTTGCTCCGATAGACCAGTCGGCTAACCATACTCAGCAC ACAAACAAGATGCGTTCGCGTCTTTCGCACCAGACGCGCAACAAGGAGACACGCACGGCGGAAGAGGAAGCACCCCCGCC GCTGGATTTGCCACAGATCAGCTTTCCGGACATGGCAAGCGCCCCAAAGTTTGATTTGATAATCAAACCCACGGTGCCAG TGGTATCAAAACCATCTACCACCGACCCAATCCAAAGTAGCAAAAGTAGCAACACCAACCTCTCTACTACAAACTCCAAG CAGATGCCAAACTTCCTGGCTAATCCCCAGCCTGCGGCACCAATTGTTAACTTCGCTGCCAATGGAAACGTTTCTGCAAT CAGTAAACCAAGTAAACGCACATTCACCTTCTCAGAACCCACACCTTTGTCGAACTTTCAGGAGAACTGCATCCCCAAGC CGAAGATCAATCGCAAATACACCTTCTCGGCACCGGCACCACTGGATGATTTGCGTATAACGAACAAACAGTCGCAGCCA ACCATAAACGGAACGCCGTCATCCAAGGAGTGGGAGTGTGATACGTGTATGGTGCGAAACAAGCCCGAGATCAACAAGTG TGTGGCCTGTGAGACGGCCAAGCCGGTAGCATCTGCGGCTCCTGTCCAAGCACCATTACCACCATCTACAGCAGCTATTG ACACACAAAGTTTCGTTGGTTTTGGGGATCGATTCAAGAAATCGACGACTGCCTGGGAGTGCGATGCCTGCATGCTGTCC AATAAGGCTGAAGCCAGCAAATGCATTGCCTGCGAGACTCCAAGGAAAACTGTTGCACCAAAGGTCAACAACTTCTCACC GTTGATTACAAATGCGAAAAGCAACGAGTGGGAGTGCTCTGTGTGTCTGGTGCGCAACAAAGTCGAGGTCAGCAAGTGTG TGGCCTGTGAGTCGGCCAAACCAGGCGCAACTATGGCTCTCCCTGCAACATCTAATATAGCTGTTGCGACTCCCTCTATA ATTACGGATGGCTTTGGGGATCGATTCAAGAAATCAGCGACTGCCTGGGAGTGCGATGCCTGCATGCTGTCCAATAAGGC TGAAGCCAGCAAATGCATTGCCTGCGAAACTCCGCGAAAATCTTCGACTCCAATAGCGAACAGTAGTTACCCGTCGATCA ACAATAATTTGCCAGCCGGATCCGGTTTTGATATTTCCTTCACGCGAAAGGCAAACATGTGGGAGTGCCAAACATGCCTG GTGATGAACAAGTCGAGTGATGAGGAATGCATTGCCTGTCAAACGCCAAACTCACAGGCGAGAAACAGCAACAGCGAGTC CGCACTGATTTCATCCATAAGCTCTTCCTCGGCATCGTTCTCCGGATCTTTATCCAGACCCTCATCTAGATCATCCTCTG GGTCAACCTCAACCTGTGGGTCAGTTTGTTCGGGATCTATCGTCAGCATTAGCTCCACCACCGAAAGCGCCAAAGCATTA TCAGCAAAAAAGGTGCCCCCCAAGCCGGATGCCGGATTTCAGCAGCTAGTGGCAGCCCAAAAAACATCAACTTGGGAATG CGAAGCATGCCTGGCAAAGAATGATATGTCGCGGAAGACGTGTATCTGCTGTGAACAGATGATGCCGGAAGCATTTAACC CTGCCGCAACAACGGCCAACTCCGCAGCCAGTTCGGTGCCCAAATTCCGTTTTGGATTTTCTCATGTAAAGGAGGTTGTC AAGCCATCTGTGGAAACGACGACCACACCAGCACCAACTTCCGCTCAATTTAGCTTCGGTTTCGGCCAAAGCAATCAGGG TAAGGATGTGGCGGACAGCAAGAAGACGGAGGCCCCGAAGACCTTTATGTTTGGCGTGAGCAAAGTGGAAGAGCCCAAGA CGGTGAGTTTTGGGACAGGCATCAAGGAAACAACGGCCACATCGAGCACAGAAGCTACAGCTCCAACTCCAGCTGCAGCA GCACCAGCACCAGTCCAGTTTGTGTTCAAGGCACCGACTACCGCAACCACGGCCAGTTCACTCACCACCACAATCAGCAC CACCAGCAACGCTCCTGCTTTGGGTGGCTTCAGCTTTGGCGCTCCATCATCGTCCTCTACAGTTTCCAGTTCGACGACTA GTACCAGTGCGAATCCCGCGGCTGTCAAACCGATGTTCAGCTGGTCCGGAGCTGGCAGTGCCGTGAGCAGCACCAGTAGC AGCCAGCAGCCAGTGGCCAAGGCGCCAACCCTAGGTTTTGGTGTTTCCTCCAGCACCGTAACAACGACAACAACCTCTAC GAAAGTTTTTGCTTTCACACCGGCATCTGGTCTAGATCCGGCAGCAGCCACCTCGGCACCAGCTGCAGGTGCCGGCTTCA GTTTTGGAAGTCAGTCAAAGCCAGCAACCACACAGAACACTGGAACCTTCTTCTTTGGCCAGCCAACAGCGGTTGCGCCA GCAACGCCAACCAACCCTAGCGTGAGCTCCATCTTCGGAGCACCGGCAACAAGTACCACTGCAAGCACCAGCGTGAGTGC CACCACCAGCACCAGCACCGCCAACGCCATTGCTTCCAGCTTTGCGCCCACCTCGACCCCTCAGCTCTTTGGCAATTGGG GTGAAAAGAAAACGGACTTGACCACCTTTGGTGCTTCTTCCGGTTCGGGAACGACTACAACGCCTAGCTTCGGCTGGTCG AGCAATGGAGATGCGGCAAAGAGCAACAGCGCTGCGGTCGGCAGTGCGGCGGTGCCTTCGTCTTCGGCCTCCACGATGGC GACTCCGATATTCGGCAGCAGTTCCATGTTTGGACCATCCAGTAGCAGTAACAACACCACCAGCACCAGCACCACTAGTC TACCATTCGGATCAGCGGCGACCACTGCCGCAACTACGCCGGCGGGCGGCAATGCAGCCCTTACTGGGTTATTCGGCAAT GTGGGCAACTCTCTAGCGGGAGTAGGAGCTCCTGTAGCCACAACACCAGCGGCAACAGCTGCTGCTCCATTGACCAACAT CTTTGGCAATCCCACGCCAGTTGCTGCTGCCGCGCCTGTCTTCGGCAGCGGAAGCACCATCCCAAGCGCAGGATTTGGAG CGCCTGCTGCAGCTGCTCCGTTGGCGGCCCCAGCGTTACCAGGAGCATTTAACTTTGGCGGAGCAACTGCAGCCACGCCA GCTGCCTCAAGTGCTCCATTTGTATTCGGAAGCAGCACCAATGAGCCGCTGGCCAAACCGTCGTTCAACTTCACCGGCTC GGCGGCCAGCTCGACTGCTCCTGCGCCAGCCTTCAATTTCACGGCCAATACGGCGGCCACTAATAATCCCTCCGGTGGCG ATTCCACACCGAATGCCAATGCACTGTTCCAATTTAGCGCGACCTCCACAGCGCCAGCCAATATATTTGCCTTCAATCCG CCAGCTGCGGGCAACTCGGCGCAGAGTTCCCAGACGGCTCGCCGCAAAATCCGCGCCCCCGTACGCCGTTTGCCGCCCCG GTAGAAATGGCATCGCAGTCGGCCATCACGATAGCAAATGAGGCCGCCGTCGGATTAGGATTAAGATATTGGCAGCCATT GCCGAGAGTAACAGAATCAGCAGTAGCAGCAAAAGCATCAAGGGCCGAAAAGGAAAAGCAAGACACAGAAGACGCCGGCA ACAATAGTGGGCTCAAAGGTTCGGTCCAGAGCCAATATCACCAGGAGCCAGATATTCAAGGATCATCGAGGATAAACATG CAGTCACCAAAGTCGCCATGTGTGAACAAATACCGCTTCGTACGCCGCCACAGCCTGAACTCTGAAACCAGGAAACCCTT TAGCAGGAATATTTGGCAGAATACAACAGGAGAAGGCAATGAAACCAATGGCGACGATGTCCCTAAAACACCAACAACTG GCGACCAGGAGGACAACCAACAGGAGGAACGAGATTCAGCGGTATATACATCAACAGAACGAACTGTACTAGTAAGGACC CAATTCAAGATAGTGCGGCAAAGGAGCGTCATCTCGAAACCGAGACAGAAGCCTCCGACTTACTCTTAAAAAGCTAAGGA ATAGGTTCGTTCCACATCCTTCTCTGCTCTCCGATGATTGGGAGATGACCACGCGAATGTCGCTCATACTACGCATATTG ATGATTACAAATTATAAAAACACTTCGTTGGATTTACGCGAGAATTCATCGCACCAAAAAAAAAACGAAAATGAATATAT TTTAACGTTATTTTTGTTGAATATATAATATACCTTAACGATAATTATGTAATAATTATTTTGCGAAATAGTAAAGAAAC GTGTAATGCTGCAATCAAACAGAGATGCGAGGCCATGTAATAGCGCAAAAAATCAACTACTGTATAATGTAGTAATACGT ATATTCGAGTTATTGGCCGATACGTCGAGCATATGATGATTGCAGTCAGATAAGAGCGATCGGCAAACGATTGCATTAAG TCTTTAGTTTTTGTTATTAATTTTAGCGTTTAGATTCACACACACACAATTCACACGAGGAAAATACCTGCAACGACGTT CTGTGCAGCAGCCAAGGTGGAAAGCCAACAAATTAAAGAAAAACAACTATCTAGCAACGTAAAGAAACCAACCAACCCTA TAGCTAACAAGATAACAGATTAGATGAAAAGATTAGAGAGAATGGTGGCAAACTGGAAAACCGAAAGAGTTATAATTGTA TGCATGTTTTTTATATACATAAATTCATAAAGAACATTTAATATTTTATTTTAAGTTTATATTGATCATTTTTAAGTCGA GAACAGAAATGGAAATCAGAAGTCTATGTTGAGAGAGAAACGGTATCATAATGTAAATAAGTAAATATATGCGGTATGAT AATTTAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.