FB2026_02 , released June 18, 2026
Clone: Dmel\LD25561
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General Information
Symbol
Dmel\LD25561
Species
D. melanogaster
Name
LD25561
FlyBase ID
FBcl0165673
Feature type
cDNA_clone
Computed gene(s)
Dmel\ND-30
 
Collection Status
DGC gold BDGP
Known Problems
aberrant intron
FlyBase assessment
  • caution: significant strain polymorphism(s)
Library
LD_pOT2_cDNA
  • LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pOT2 vector.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage
    organism

    Comment: 0-22 hr AEL

    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    950
    NCBI
    AY118532
    Full Length sequence dataSequence Downloader
    ACATTTTAGCATTACACATTGTCAAAAACGGAGCAAATTGTTGAATTTATATTGAACAGTTTGTAGAATGGCGGCCTTAA
TCAGGAATCTGGGTGCCCGTGCCGCAGTCGCCGCTCTGTCGGCCAAACATGTGGTGCCCGCTGCTGGATCCACTGCTCTT
CGGATGGCCAGTACAACGCCAGTGGAGCCTAAGAAGGCGGATAAGCCCACTGTCCGCCAGCCGGACGCAGTCGCTCGCTC
GCATCTCTCCGATTTCGGGCGCTATGTGGCCGAGTGCCTGCCCAAGTACGTGCAGAAGGTGCAGCTGACCGCCGGCGATG
AGCTGGAGGTGCTTATTGCGCCAGAGGGCGTGGTGCCCGTGCTGCAGTTCCTTAAGGATCATCATCAGGCGCAGTTTACC
AACCTGGTTGACATTGCCGGCGTGGATGTGCCCTGTCGCAAGAACCGATTCGAGGTGGTCTACAATTTGCTCTCGCTGCG
CTACAATTCGCGCATCCGCGTTAAGACCTACACCGATGAGCTGACTCCTCTGGACTCCGCCTGCGAGGTGCATAAGGCGG
CCAACTGGTACGAACGTGAGATCTGGGACATGTACGGCGTGTTCTTTGCCAACCATCCCGACTTGCGCCGCATCCTGACC
GATTACGGTTTTGAGGGACATCCCCAGCGCCGCGACTTCCCGCTGTCCGGCTACGTAGAGCTGCGCTACGATGATGAGAA
GAAGCGAGTCGTCTGCGAGCCCTTGGAGTTGGCCCAGGAGTTCAGGAAGTTTGACTTGTCGGCGCCTTGGGAACAGTTCC
CCAACTTCCGCAACGCTAATCCCCCCGCCGAGGTAGTTCCGCCACAGGCTCCAGCCAAGAAGTAATCCGAAGTCGGTTTG
TTAAATGGTAATTAGATCAATAAAACGTGATCACTATGAGATGTTTAAACATAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: LD_pOT2_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.

    (Rubin et al., 2000)
    Sample preparation

    Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

    (Rubin et al., 2000)
    Comment
    Reported As
    Symbol Synonyms
    LD
    (Rubin et al., 2000)
    LD_pOT2
    (Rubin et al., 2000)
    LD_pOT2_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)