Comment: 0-22 hr AEL
ATTGTTTTTAGGACCTAGGCTAGGAATTACTGGTTGCACACACTCAGCGCCACAGCTCCACCGACCACCGCCCTTGCAAG GACCCACCATGAACTCCAAGGACAAGTCCAAGTTCAAGTTGTTCCTCAAATCGCTGCCGGCAGGTTACGTGGGCGAGCGG ACCCTGCGGCCGGAGTTTGAGAGGGAACTGCGTCCGGAGCAGCCGGTGGCCCAGCGCTGCCGGATGCTGAAAGAGCTGGG CGACACGCAGCTGCACAACTTCAATCTGGACGAGTCCGCCATCACCATTCTGTTCAATCTCACCAACGATCTCATCGTGC CCAACAAGCCGGCAGAAACGCGGCAGATTGCCTTGAGCTTCTATAAACGGCTCATTCACACACAGTACAAGAACCTGACC ATCATGCGGGAGAAGTTCTTCCTGGTCATCCAGAACCACGAAGCCCGCGAAGATCTGCGTCACCTTCTTGAGCTGCTCGA CACCCTCACCGACAATGGGAAGGATATCACAAACTTCGAGGAGAAGATTGGCAAGTTTATGCTGCTCTGGATCCCAGCCA TTACGGAGGCCAATCTGCTTACCCCCTACCTTGACATTCTGGTCAACCTGATCAAGTTCAATGCGGCGCATCTGGACAAA GACATCCTTGTGGGCATCGTGCAGAACGCTTGCGATCTGAGCTGTAGCGTTACCGTAAACGAAATCGGTCTGCAGTGCCT CACAATTCTGGAGATGGTCATCGGGTACACCATCTTCCCAAGCGAACCGCTGCCGCAGTGCATCACCACCTTGTGCCGGA CTGTGAATCATGCTCCTTACTGCCCGTCTTCTTTCAAGATCATGAAGAACCTCTTGGGCACCCAGTTGGGATACCATTCC ATGAAGATGATGTGCAGTATCCTCAACGATCGAGCTCTTTACGATGATGCCCACCTGTTGCGTGGGGCCGTGTTCCATCT AAACATGAACATCTTTGGCTCGAACATTATTTTCCAGGTTTCGCCTATGACCTATGCAACGAATGTGCTGACAGCCTTCC TGCGGGCACTAGACAGTCGTCAGGTGATCGTGACCTTCGAAGTCATCCTCAGCGTTCGCATGGTGATTACCAAGCGGCAG CTTTCAGAGATCATTTGGGATCTGATCTGCGATATAATGTCCTCGATCGTTAGCAATATCGAGTATTACGAAGAAGTCAA TATAAACAAGGATCGTCTGCATCACCTGCAGATCAATTTCCACGAGAATATCGACTGCATCGAGAAGCTGTTGCAGCAGG ATAGATCCCAGATTCTGGGCAATGTGGAGCGCATCTACGATCTCATCGAGCGAGTTGCCGACCGACGCCCAGAGGCTTCC GTTCTAGCCCTAATCGAGTACCGATCCCGACGGGTTACAGCAACGCGGCCAGATTGGCTTCAGGTGCTGGCCCAGTTCGT CCGGCGCTACTACCGCATGTCCAACGTTAACGTGCGCATTAAGACGATCGAGGCTCTAGTTCAGATTATGGATCAAAACC GTGCCTGCTATGAAGAAGAAATCCTTAGTCGCGTGGTGCTGGTGCACCTTTCCCATATTCATCTGGAGTCGAGTGTGCAG GTGCGAGTGGCCGTGGCCCGGGCTCTTTCCAATTTTGCCACCCATTGCGACACGAAACGGTGCATGGATCTGCTAGATAT CCTGGAGGCGCTCATCAATCGACCCTTTGAGCACACGCGGCATGGATCCTCATCTGGAGAGGGTACACTTGCAGAAGTGA CCTTTGGATTGGTTAACAACGAATCCGAGATCTCGGATATTATCGCCGCAGTTGATGGTTTGGTCAAAGTGTTTGCCATC AAGCTGCATCGTCTGCCAGGGATCCATGCTCTTAAAATATTTAACATTCTAATGGACCACCTGGAACTGCACTACGAACG ACCAAAGATTTTCGAGCACATAAGTGTTGTGCGCTATAAAATATTTGCTTGGTTGCTGAAGGCCCGGGCCAACGGCTCCT ACCATATTGGTTATCCGGAGGGCAGCACCGAGGTGGTGAAATTTAGTCCATACCTGGGAATTGATTCACCACTGTTACCA CAAGCCCACCCGACAGCGATATCCATACGACGAGTCTGCAAGCTGATAGTGAGGTGCCTGGAACACGACACCGATTATCA GGTGTTCCAGCTAGTCATCAGGGAACTGCCGAAGGTGCTGCAGAACAAGGCGCTGGTGCAGGGCAATGACATCGAGGAGC TGGCCAACACGCTCCTCAAGATCAATTTGGTTAGCAACAACAAGTTCAAGCGACCCACCGATGAGTTCCACGCCCTCGTT TTACCCGCCATAGCTTCATTGGTTATCTATCACGAAAGCCTGCAGCCGCAGCAGCACTACGGAATTATAACAGCTCTCAA TTCGAGGGTACTTACTGGCATTGCCAGCGTCTGCATCAACACCATGACCATCCTTATACTAGAGATGCCGGAGGCGTTGA TGCGAAAGCTGCCGGACGTACTGCTCCAAATGAGTAAGATGTCGGATACGAATGCGTTGGCCACTCCGGTTCTGGAATTT CTGTCGTTGCTCATACATCTGTCCAAGCATCTCTTCGCAAACTTCACTTCCATGCACAACATGTACGTGTTTGCCATCAC GCTGCCGTACACCAAACCGCATCGCTACGATCACTATACAGTTTCCTTGGCCCACCATGTCATCGCTGGATGGTTCCTTA AGTGTAAGCTGGAGCTGCGCAGGAACTGCGTTAACTACATCAAAAGCTCGATTCAGTCGAATGCCAAGATGTTGTCCAGC GACATTGTGAACCTAAACTCCTTAAACGAGGATTCATCCAATCGCAAGCGGAGCACCAGTCTAACTGAGCGCGGTAGTCG CAACAATGCCAATGCCTGGAACGATCTGGAGATGCGACCGCAAATGAACAACGGTCTACGAAACTTCCACGCCGAACTGG CAGAGACGTGCTTTGATTTCTTGGCGCGACACACTTATTCGCCTTGTCCGTCGATGCCCAAGCGTCTTCCAGCGGCGGAA TTCCTGCTGAAGGACGGCGTCTCGCAGACGTGGCTAGTGGGCAACAACCTGGTGACCATAACCACCTCCGGCTGTCCGTC GGCGCCCGCGCGCAGTGGGCTTTGCGAACGATGTGCCCAGCTAGGAAAGGCTCCCAGCATTAGTCTGAACTCGAAGAGCC TCAGTGATGCAGCTCCTCCACTTTCGCCAGAGCGGGAGAGACGATACACCAAGGTGAGCCTGCAGCACAGCAGCGGCAAC GAGTCGGCTGGCAGCACGGAGCTCACCTCCTCCTCGTCGTCGAACTCAGCAGCCGCAGGTGGTCATCCACATCGACAGAT TTCTAACAGTTCCACGGCATCGCTAGACGCGCTTTCCCGTCGGGGGTCTAATCCCGAAGCCTTGGGCAGTGCCTTGGGCG AGGGGGCACATACGGGGAGCAACACCTCGCTCCTGGGAAACTCTTTGTCCCAATCCTCCGTTAGCATGAGCCCTTCGTCG GGATCCGTGGTGCAGCAGCCGGTTTGCGTGCGCGCCTGCACTGGCTGGGCGGAGGTTTTGGTTCGTCGGCCCACGGGCAA CGTATCGTGGATAACCCGCATTCAGAACCCCATCACCAACGATTGCTTTGGCCAGGAGTTGCCGTTCAATAATGTGGTGT CGCTGTTTCTTCCCACTGCCCATGGCGGAGTTTTTGGACCTGACAATACCATTCAGGCCCCACCACATACGTTGGCTGAT CCCGAACCAGAACCAGAAGCAGAGGTTAATGCAGCAACAGCTCCTCAGGCAAGTGCATTGCGCAGCCGAATGGTTGCCAA GGCTCGTGCCCTTCAGCGACAGGAAGAAATTCACTCGGTCGGAGGAAATGGAAATGGCAACGGCAACGGTAATGGTAGTG GAAATGCTCCCAGCACTGGAGGTGCTGCTGCCATACCCATTCCGCGGGTCCCAGCATCTGGAAAACGCGGCAAGGAGGCA GCTCTTTGTGGAAGTGTGAGTGATGGTGAAGCCGACGATGACTCCCTGGCCTTTGAGGATGCTCAGAGTCGGGCACGAAA TCCCGTCCGACGGGTAAATTCCAGCCCAGAGATGAGTTCCAGTTGGCGGCAGTCGTTCCTAACCAACAAACCCACGCCAC TGTCCCAGGAGCCCGTGAAAACCACCGCCGATGAACCGCAATCGCTGTTGACTTTGAAGAAGAAGACTGTTCAGTACAGC ACCAAGGATATGCGTGTGAGCTGTGAGGCCATTCCGGAGGAGATCGCCGGCTCCACACCACCGTCGCAGGCCGCAGCCTT GGCCCTGCAGCCGGAAAGCGGCACCCTACCTCCCAAGCAGCATTCGGCGGACGATGTCAGCAGTCATGTGGCTGGAGGAA GTAACTTGCAAGCAGGAGGGTCCACGCTAAAGCTGGGTAAACCACCGCTGTCGCCGGGGCAACCGCCTCTGCTGGGCACT GGTCGAGTGACCAGCTTTGGTGGCACCTCTGTTCCGCAGCCCGGTGCACTGGCAAAATCGAGCAGCAGTGGCAGCAATGG CACCAATGTTGGAGTGATAACCTCCGATTACGACAACGGCAACAATGGAAACGGAGACATGATGCGAGGTCGTTCGAAAA CCATTTCGGTGGTGCGTGAAGTGAATAACGGAAATACGCGTCCGCCGCCGGCCAGCAGTTTCCGGAATTTCGGAGCCGCA AAGCCGCCCATCAACACCAAGCTGTGCATGAACCCGAGCTTTATTTTCATGCAACTGTACACCACTGGCCAGCTGGGAGT TACGGATGAGCCTCTAAAGGTGGGTCCGGAAAACAGCAGCGCTGTCTCCCTTATCGATTTAGTCCCACCGTTCGAGACGC ACAAGATCGGCGTGCTGTACGTGGGCCAAGGGCAGTGCAACAACGAGGTGGAGATCTTGCGCAATTCGCACGGAAGTGCC AGGTATGTGGAGTTCCTGCGTAACATCGGCACCCTGGTCAGTCTGAAGGAAGCTGAGCAGAACAATCTATTTATAATGCT AGATAGAAACGGAGCAGATGGCAAGTTTGCCTATATCTGGAAGGATGACATACTGCAGGTTACGTTCCATGTCGCTACGC TGATGCCCACCAATCTGCAGGATGATCCCAACTGCAACGAGAAGAAGAGCCACATTGGCAATGACTTTGTGAAGATCATC TACAACGAAAGCGGCGAGGAGTACAATCTAAACACAATATCCGGCCAATTCAACTACGCCTGCGTGATCGTTGAGCCACT TGACCTGAACTCGAACAGGGTGTATGTGAAGGCGCGATCGGAGATTTCGAAATTCGTGTGCCACGCAGAATACCGGATAG TGTCCGATCGCAGTGCTCCTCTTTTGGCCCGACAGATGGCGCTGCACGCCAATCTCGCGTCGCTAGTCTACCAAAGTGTG CAGAAAAAGCATCCGTACGCATCGAATTGGCTAGAACGATTGCGCAAACTAAAGCGCCTGCGATCGAAGCTAATTGAGGG CCTAAACAGCCAGCAAAAATCCGGAAGCGCCTCGAGCGGCATCGGAATCAACGCCTCAGCCATTGGTTCGGATATGGACG ACCAGCGAGGTGACTTCATTAAATACACATAGACAACAACGAGAGGCTCCCAACTGGACAACTATTAAAAAAGAGCAGAG GACCTATAGACGCATCCTGCCATGATATCTCTTGATTTACGATTTCAATATAAAGTCTCTCCAGACCAACAAAAGAAAAA AAAATTAAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
