Comment: 0-22 hr AEL
TGTTGCGCCACATTAACAATAACAATTGATGCGCCCCGCCACCGCCCACTAGTCGCGAAAAAAGTGAATTATCGGTACCG CGGATTAATGTGCCACATGCGAGAAGCGTTGCAACGTTGCGCCGTCGTCGGGCGTCGAACAAAGCAGTTTTTGGGAAAGT GAAAACGGCGCCATTTTAGAAGCAGGCAAGCCACGGCCTAAGGCTCAAGAAAAGTGCAAATGCAATGTGTGCAGCAATAG ATTGTCATTTCGGCACAAACGAAACCGACTACTGAGGTCTACTACTAGCCCAGAAAACAATCAAGCCTCCCCAGCGACTC CAAAATATGAGCTGTAGCCAAAATGAGACGGCAGCAGCAAAGGTTCTGGAAACGCAACGCGCACAAGAATCCGGCAGTGA AAATGAGGAAACCGATTCCATTACGGATCAATCGAGCCAGTCGAAGTCGATCAAGTCAGCCACCCAGTTTAGCGTGCAGC GTTCGGACACCGATGGACTGCGAATGAGAATCTCGGCCATCCGCCCCACGTTAGGAGTCGTAGCAACCAAGAAACCCCCA AAGTCCAGAAAAATGTCTACCCAGGACACCGAGTCCGGCTGCTCGGAGGCCAAAAATAGAGCGGTCAGCAAGAAAGTGAA GGTCAAGCGCAAGAAGCTGGCAAGTTCCAGTGGGATTAGTAAATCGGACAAAGTGTCTAAGTCTAAGAAGTCACAGATCT CGGCATTTTCCTCGGACTCAGAGGACGATCTGCCCTTGAAGGTGCATCAGCAGAGAGCTCCGCGAGTGCTGCTAAGTGCT ATCATCCAGGCGGCACAGTCGGCCAGCAAACCCACCCTCGATATCGGAATCTCGTCCAGCGACAACGAGTTACCTAACCT GGTGCAGGCGGCTATCAAGCGAGTGGAGAGCGATACGGAGGACACAACTGTGGAAGGAAGTTTCCGCAAAGCGGCCAAGG ACAAGAACCTACCCCAGTACCAGTCAACTTTGCTGCAAGACTTCATGGAGAAGACTCAGATGCTGGGGCAGACCGTCAAT GCGAAGCTTGCGGAGGAGAAAGTGGCTAAAGCTAAGGAGGAGACCCTAGTCCAAACAGCTGTTCCTCGAAAGCGCAGAGG TAGACCCAAAAAAGTGGTTCCCACAGTTCCTGCCCCAGGAAACTCTGGCCCTGCTATAAACGAATCTGCCGATTCGGGTG TGATAAGCACCACTAGCACAACGCAGAGCACTACTCCCTCCCCGAAAATGCAAAATGAGAATGCTGTGCCGACGGGATCG CTGCCAATCGCCTCCAGCAGCAAGCCTAAGATCGACATGGCGTATCTGGACAAGCGAATGTATGCCACTGAGCGGGTGCT CTATCCACCGCCCAGGAGTAAGCGACGGCAGAACAATAAAAAGACAGCCTGCAGTTCATCCAACAAAGAGGAACTTCAGC TTGATCCGCTGTGGCGAGAGATCGACGTGAACAAGAAGTTTAGGCTGAGGAGTATGAGTGTGGGTGCGGCTAGTGGAACA GGAGCAAGCACCACTATTTGCAGTAAGGTCTTGGCCGCTAAGAGCGGTTACGTCTCGGATTACGGAAGCGTACGACATCA GCGGAGCAGCCACAACCACAACTCCGGTTACAAGTCCGATGCCAGCTGCAAGAGTCGATACAGCACCAAGAGTTGTATGA GTCGCAGGAGCAGGGCAAAGAGCTGCGGCTACCGGAGTGATTGCAAGGAATCTGGAAAGTCAGGCCTAAGGATGAGGCGG AAGCGCCGGGCTTCCATGCTCTTGAAGAGCTCAGCAGACGATACTGTCGAGGACCAGGACATCCTTCAGCTAGCTGGATT ATCTCTGGGCCAGAGCAGTGAGGAGAGCAACGAATACATCAGTAAGCCGAGCCTTAAAAGCCTTCCCACGACAAGTGCCA GCAAGAAGTACGGCGAAATCAATCGCTATGTGACCACCGGGCAGTATTTCGGTCGAGGCGGTAGTTTGTCTGCCACCAAC CCGGATAACTTCATTAGTAAAATGATGAACCAGCGTAAAGAAACCCCGGCTCCCAGCAAATCGTCCTGCAAGATTAAATC TCGCCGCTCATCGGCAGCCAGTATGTGCAGCAGCTATGTGTCTGGGGTGTCTAGAATGCGTCGCAGACATCGCCGGAAGA GTTTCAGTCACAACAAATCATTGAACATTGATTCCAAGCTGCTCACCGAAATCGAGATAATCACAAGCACCTTCAACTCA AGGTGTCGCATCCAAGATGATCGTTTGACCGGAAGCAGTGGCAAAGAGAAGCTATTGGCCGATGCCAACAAGCTGCAGGC CACCCTAGCAGCACCAAGCCCTGCCCAGCAGTTGACTCTGAATGGAGGAGGACCAGCTTCCACCCTTTCCAAACCATTAA AACGGGGCCTAAAGAAGAGAAAGCTGAGTGAGCCCCTAGTGGACTTCGCTATGCTATCAGCTAGCGCTAGTGGAACTCCC AATGGCAGCGGAAGCAGTAATGGAAATACCAAACGACGACACAAGAAATCTCAAAGCAATGACAGTTCCAGTCCCGATGA TCACAAGTTGCCGTTGAAAAAGCGCCACTATCTTTTGACTCCAGGAGAGCGTCCGCCGGCGGAAGTAGCATTCGCCAATG GGAAATTGAACGCAGAGGCCTGGGCTGCAGCCGCGGCGGCTGCTAAGAGCACTGCGTCTACCAAATCGCAAGCCCAGTTT AATGCTAGGAGCGTAAAATCTGCGCTAACGCCCAAAAAAAGACATCTCCTAGAGCAACCTACGTCTGTGAGCGGAGCTGG CTCGTCGGCCAGTAATTCTCCCCTGAGAATTGTTGTCGATAATAATTCCATAAGTGGTGGAAAGTTGCTGGATATAAGTC CTAGTTCCTTGTGTTCCCTCAAACAGCAGAGGAGAGGAGGAGCAGCTAAACAGAAGGTGTCGGCAGCGAAGGACCTCGTT CAGCTTCAATCCCCAGCTGGTAGCTATCCTCCCCCCGGTGTGTTCGAGCCATCTGTGGAGCTGGAAATCCAAATTCCTCT TAGTAAACTGAACGAATCCGTCATAACCAAAGCAGAGGTCGAATCTCCACTGCTCTCAGCATTGGACATTAAGGAGGATA CGAAAAAGGAGGTTGGCCAGCGCGTTGTCGAAACCTTGCTACACAAAACGGGAGGCAATCTTCTTCTGAAACGCAAGCGG AAAAAGATAAATCGAACTGGATTTCCCACTGTTCGCAGGAAGAAACGTAAAGTTAGCGTGGAGCAGCAAACAACAGCCGT GATTGATGAGCACGAGCCGGAGTTTGATCCCGATGATGAGCCACTGCAATCCCTAAGAGAGACCAGGAGTAGCAATAATG TCAATGTGCAGGCGGCACCTAATCCTCCACTGGATTGCGAGCGAGTTCCCCAAGCAGGCGAGGCCAGAGAAACCTTTGTG GCCAGGACCAATCAAAAAGCCCTCGATTATCGGTGGTGGCCCTGGAGCGCCTACAGCGTCCTCAAACACCAGCTAGAGGA AGACCGCGAGGTAGAAAACCTAAGAACAGGGAACAAGCTGAAGCTGCACCTCAACCGCCGCCCAAATCGGAACCTGAGAT AAGGCCAGCCAAAAAACGTGGCCGGCAACCCAAGCAGCCGGTACTGGAAGAGCCACCACCCACACCACCTCCTCAACAGA AAAAAAACAAAATGGAACCAAATATTAGACTACCAGATGGCATCGATCCCAATACGAATTTCAGCTGCAAGATTCGCTTG AAGCGGCGAAAGAACTTAGAGGCTGGAACCCAACCAAAAAAGGAGAAGCCAGTCCAGCCAGTGACGGTGGAAGAGATTCC ACCAGAAATTCCCGTCAGTCAAGAAGAAATAGATGCAGAAGCAGAGGCTAAACGGCTAGACAGTATTCCTACCGAGCACG ATCCCTTGCCTGCCAGTGAGTCCCACAACCCCGGTCCGCAGGACTATGCCAGTTGCAGTGAATCCAGCGAGGACAAGGCA TCAACCACATCCTTGCGGAAACTATCTAAGGTCAAGAAGACCTATCTTGTAGCAGGACTCTTCTCGAATCATTACAAACA ATCCCTGATGCCGCCCCCAGCAAAGGTGAACAAAAAACCAGGCCTGGAGGAGCAAGTGGGTCCTGCGAGCCTTCTACCAC CACCTCCCTATTGCGAAAAGTACCTCCGAAGGACTGAAATGGACTTTGAGCTACCTTACGACATTTGGTGGGCTTACACT AATTCCAAATTACCCACTCGAAACGTTGTGCCATCCTGGAATTACCGAAAGATCCGCACCAATGTATACGCAGAGTCAGT GCGTCCAAATTTGGCGGGATTCGATCATCCCACCTGTAACTGTAAGAACCAGGGCGAGAAGTCTTGTTTGGACAACTGTC TCAACCGCATGGTTTATACGGAGTGCTCGCCCAGCAATTGCCCGGCTGGAGAGAAGTGCCGAAATCAGAAGATCCAGCGA CATGCCGTCGCACCCGGAGTGGAGCGCTTTATGACGGCAGATAAAGGATGGGGAGTGCGAACTAAGCTACCCATTGCGAA GGGAACCTACATTCTGGAGTACGTGGGCGAGGTAGTCACGGAAAAGGAATTCAAGCAGAGGATGGCCAGCATCTACCTAA ATGACACCCACCACTATTGCTTACATTTGGATGGAGGACTGGTTATCGACGGTCAGCGGATGGGCAGCGATTGTAGGTTT GTCAACCATTCTTGCGAACCCAATTGCGAGATGCAAAAATGGAGTGTTAACGGCCTATCTAGAATGGTTTTATTCGCCAA AAGAGCCATAGAGGAGGGGGAGGAGCTGACATACGATTACAACTTTTCGCTGTTCAATCCCTCAGAGGGTCAGCCCTGCA GGTGCAACACGCCCCAGTGTCGTGGTGTCATTGGTGGCAAGTCGCAGAGAGTTAAACCCCTTCCTGCTGTGGAGGCCAAG CCATCTGGAGAGGGACTTTCTGGCCGAAACGGGCGCCAACGGAAGCAGAAGGCCAAAAAAGCATGCTCAACGGCAGGCGG GAAAAGATATTTCATCAGCAGTGGCGGTGGCAAAGCTTCAACCATTGTCCGAAAAAGAAAAGAAACTGGTCAGACAATTC AACACATTTCTAGTCAGGAACTTCGAAAAGATACGCAGATGCAAGGCCAAGCGGGCGTCAGATGCAGCGGCGACTGCATC CTCGCCAGCACTTGGCACCACTAATGGGGACATTCCTGGTAGACGTCCCTCCACACCATCTTCTCCTTCCTTGGCAGCGC AGATTTCCGCGCTCTGCTCGCCTCGCAACATAAAAACCCGTGGACTCACACAGGCAGTGCATGATCCCGAACTAGAGAAA ATGGCCAAAATGGCTGTAGTTCTAAGGGATATTTGCAGTGCCATGGAGACCCTCAAAATGTCCGATTTGTTGACGACAGT GTCCAGCAAGAAAAAGAAGCCTATAAAGACCACTTTGAGTGGAAAATTGGGTTCTACAGCTGCAACTTCAAAAGTGGAAT TCAGATCGATACAAGCCCAGGTGGAGCAGGGACATTACAAAACGCCGCAGGAATTCGATGACCACATGCAGCAGCTCTTT GTGGAGGCCAAGCAGCAACACGGCGATGATGAGGGCAAGGAAAAAGCGCTGCAGTCCCTGAAGGATAGCTATGAGCAACA GAAGATCGCCAGCTATGTTCAGCTGGTGGAGATTCTTGGTGATTCGGAATCGTTGCAGAGCTTTAAACCAAAGGAAGTGC TTTCGTCAGAGGAGGAACCTGGAAAGATAGCAGTGAAGAAATCACCAGGAGCAAAGGAAAGAGATTCCCCAATTGTGCCA TTGAAAGTGACTCCGCCACCCCTGCTTCCTATTGAGGCCTCTCCTGATGAGGATGTAATCCGATGTATTTGTGGATTGTA CAAAGATGAAGGCTTGATGATTCAGTGCTCCAAATGCATGGTGTGGCAGCATACTGAATGTACCAAAGCTGACATCGATG CGGATAATTATCAGTGCGAGCGTTGCGAGCCAAGGGAAGTGGATCGAGAAATTCCCCTGGAGGAATTTACCGAAGAGGGA CACCGTTACTATCTCTCCCTAATGCGTGGTGATCTGCAGGTACGACAGGGCGATGCCGTCTATGTCCTACGAGACATCCC CATCAAGGATGAGTCCGGCAAGGTGTTACCGACGAAGAAGCACACCTATGAAACGATCGGAGCCATTGATTACCAAGAGT GCGATATCTTTCGGGTGGAGCACTTGTGGAAAAACGAACTGGGAAAGCGTTTTATATTCGGACACCATTTCCTGCGTCCT CATGAAACTTTCCACGAACCATCGCGTCGTTTCTACCCCAATGAGGTGGTACGAGTTTCACTTTATGAGGTGGTACCCAT TGAGTTGGTCATTGGACGCTGCTGGGTGTTGGATCGAACAACTTTCTGTAAAGGACGCCCCATGGAATGCAACGATGAGG ATCATTGCTACATCTGCGAGCTGCGTGTGGACAAGACGGCAAGGTTCTTCTCAAAGGCCAAGGCCAACCATCCAGCCTGC ACCAAGAGCTATGCCTTCAGAAAATTTCCCGAGAAGATTAAGATCTCCAAGAGCTATGCGCCCCATGATGTGGATCCGTC GTTGCTGAAGACAAGGAAGCAAAAGACTGAATTAGACGTAGGAGCCGGGCCAACAACGATGCACAAGGTGTCTGGCAGGC AGGAACAGCATCAGGCCAAGATGGTTGGGCGAAAGCCTCGTGGGATCAGTGCCCCAGCGGATGCAACTGCTGTCCATGTC GTAACACCCGTGGCGCCCAATAAACAGATGCTTAAGAAGAGGAAATCGCGTTTAGAGAACGTTTTGATAACTATGAAGCT TAAGTGTCTGGATGCACAAACGGCACAGGAGCAACCCATTGACTTGTCATACCTGCTGTCCGGACGCGGCGCCCGGCAGC GGAAGACCCAGCAGTCCAGTAGCAGTTCTACGGCCAACTCAACATAACCGATGTAACGCGAGTAGATTAAGCCGTGTATA TACGCCTAAAGTTAGAGGCATTGATTAATATTTATGGAACTTCAATTAACAATTAGCATTTAGCCATGTAAGATGTAACG GTGATAGTAGTAATCCTAAAGTGTACATTGTTATGAACAAACTTCGTGAAAGGACTCCGGTCCCGGCTAGGATGGAACAG ATTATGAATTATGGTTAATATAAGGATTTATTTAAATACTAGTGCTTACGCCACCGCACCAAGCCCCCAATTTAGATGAA TTTCTTTTGTTTTTAATTGTTTATATTGGCTCTCCATATTAATTTTGTTTTACAAAACTATCCCCCTCTCGTCTTTGCAA TTGAAGGCGTTTGACTTTTGCATTGTTATTTTAAATTATTGTATTATGTTATGGCTGCTTAATTATGTAACACCCGAGTA GGACAACTTATTGCGCTATTAGTTTTTAGTTATTTTCCAATTGTTATTAACTGCTAACAAAAATTTTAAAAGTGAAATAA AAAATATATTTTTATTAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
