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FB2026_02
,
released June 18, 2026
Comment: 0-22 hr AEL
CTGAGCTCGTCCACGATGGTTATTACTTGGGAACCACCAGAGACGCCCAATGGACAAGTGACCGGCTACAAGGTGTACTA CACGACCAATTCGAATCAGCCGGAGGCGTCGTGGAACTCCCAGATGGTCGACAATAGCGAACTGACCACAGTCTCGGAGC TGACGCCCCACGCCATCTACACGGTCCGGGTTCAGGCCTACACATCGATGGGAGCCGGTCCAATGTCCACGCCGGTCCAG GTGAAGGCCCAGCAAGGTGTGCCATCGCAACCGAGCAATTTCCGGGCAACCGATATCGGCGAGACCGCAGTCACACTGCA ATGGACCAAGCCGACGCATTCCAGCGAGAATATCGTGCACTACGAGCTCTACTGGAATGACACATACGCCAATCAGGCCC ATCACAAGCGCATTTCCAACTCGGAGGCGTATACCCTGGACGGACTCTACCCCGATACCCTCTACTACATCTGGCTGGCT GCAAGGTCGCAACGTGGCGAGGGGGCCACCACCCCGCCCATTCCGGTGCGCACCAAGCAATATGTACCAGGTGCTCCGCC TCGAAATATCACCGCCATAGCCACCAGCTCGACGACCATATCCCTCAGCTGGCTGCCTCCGCCCGTCGAGCGATCGAACG GCCGGATCATATACTATAAGGTGTTCTTCGTGGAGGTGGGTCGCGAAGACGACGAGGCCACCACCATGACCCTCAATATG ACCAGCATTGTACTGGACGAGCTGAAGCGCTGGACAGAGTACAAGATCTGGGTGCTGGCCGGCACCTCCGTCGGGGATGG GCCGCGGTCGCATCCCATCATTTTGCGCACCCAAGAGGATGTGCCCGGCGATCCGCAAGATGTGAAGGCCACACCTTTGA ACTCCACTTCGATCCATGTCAGCTGGAAGCCGCCTCTCGAAAAGGATCGCAATGGCATCATCCGTGGGTATCATATACAC GCCCAGGAGCTGCGAGATGAGGGCAAGGGCTTTTTGAACGAACCCTTCAAGTTTGATGTGGTAGACACGCTGGAGTTCAA TGTGACTGGCTTGCAGCCGGATACAAAGTACTCCATTCAGGTGGCGGCACTAACTCGTAAAGGAGATGGTGACCGGAGTG CTGCGATTGTGGTGAAAACTCCTGGCGGAGTACCAGTTCGACCAACGGTGAGTCTGAAGATCATGGAGCGGGAACCGATC GTGTCCATCGAACTAGAATGGGAGCGACCGGCGCAGACCTATGGCGAATTGCGTGGCTATCGACTTCGATGGGGCGTCAA GGACCAAGCACTGAAGGAGGAGATGCTGTCAGGACCGCAGATGACCAAGAAACGGTTTGATAACTTGGAACGCGGAGTTG AGTACGAATTTCGTGTGGCGGGCAGCAATCATATTGGTATCGGGCAAGAGACGGTGAAAATATTTCAGACACCCGAGGGA ACACCCGGTGGACCGCCTTCTAACATTACCATTCGCTTCCAAACTCCGGATGTACTGTGCGTGACCTGGGATCCACCAAC TAGGGAGCACCGGAATGGCATAATCACCCGCTATGATGTTCAGTTTCACAAGAAAATCGATCATGGCCTGGGATCCGAGC GAAATATGACTCTCCGGAAGGCGGTGTTCACAAATCTGGAGGAGAACACCGAGTATATCTTCCGGGTGAGGGCTTATACG AAGCAGGGAGCTGGTCCCTTCAGCGACAAGTTAATCGTGGAGACAGAACGTGACATGGGTCGAGCACCTATGTCCCTGCA GGCAGAGGCAACATCGGAGCAAACTGCGGAGATTTGGTGGGAACCGGTAACAAGTCGTGGCAAGTTGCTGGGCTACAAGA TCTTTTACACCATGACAGCTGTCGAGGATCTGGACGATTGGCAAACGAAAACCGTTGGACTTACGGAATCCGCTGATCTT GTTAATCTCGAGAAGTTTGCCCAATATGCCGTGGCCATTGCGGCGAGGTTCAAGAACGGATTGGGACGTCTTAGTGAAAA GGTTACAGTACGCATCAAGCCGGAGGATGTGCCCTTAAATCTTCGCGCTCACGATGTCAGCACCCATTCGATGACCTTGA GTTGGTCGCCACCCATTCGCCTAACCCCGGTAAACTACAAGATCAGCTTCGATGCCATGAAGGTGTTTGTGGACTCGCAG GGATTCTCCCAGACCCAGATCGTTCCCAAGCGAGAGATTATCCTTAAGCACTATGTGAAGACCCACACTATCAACGAACT CAGTCCGTTTACCACGTACAATGTGAATGTGAGTGCCATTCCCTCGGATTATTCCTACCGGCCGCCCACAAAGATTACGG TCACAACGCAAATGGCTGCACCTCAGCCAATGGTGAAGCCGGATTTCTACGGCGTTGTTAATGGCGAGGAAATTCTGGTG ATACTGCCTCAGGCTTCGGAGGAATATGGACCCATATCGCACTATTATTTGGTGGTGGTCCCGGAGGACAAGTCCAATCT GCACAAGATACCCGATCAGTTCCTTACCGATGATCTCTTGCCGGGCAGGAATAAGCCAGAGCGTCCGAATGCACCGTACA TTGCAGCCAAGTTCCCGCAGCGTTCCATTCCGTTCACATTCCACCTGGGATCTGGCGATGATTATCATAACTTTACAAAT CGCAAATTGGAGCGAGAGAAGCGCTACCGCATCTTTGTGCGAGCGGTTGTGGATACGCCACAGAAGCACCTCTACACCTC CAGTCCCTTCTCTGAGTTCCTATCGCTGGACATGAGGGAAGCTCCGCCAGGTGAGCGGCCCCACCGACCCGATCCCAATT GGCCCGCGGAGCCGGAAGTGTCGGTGAACCGCAACAAGGACGAACCGGAGATTCTGTGGGTGGTGCTGCCCCTGATGGTG TCCACATTCATTGTGTCCACCGCCCTGATCGTTCTCTGTGTGGTTAAGCGTCGTCGCCAGCCGTGCAAGACTCCGGATCA GGCAGCTGTCACAAGGCCACTGATGGCCGCCGACCTGGGAGCCGGACCTACGCCCAGCGATCCCGTGGACATGAGGCGCT TGAACTTCCAGACACCCGGCATGATCTCCCATCCGCCCATACCGATATCCGAGTTTGCCAACCACATCGAACGACTCAAG TCCAATGACAATCAGAAGTTTTCGCAGGAATACGAAAGCATTGAGCCGGGCCAACAGTTCACCTGGGACAACTCCAATCT GGAGCATAACAAGTCTAAGAATCGCTATGCAAATGTTACCGCCTACGATCATTCACGCGTCCAGTTGCCAGCGGTGGAGG GTGTGGTTGGATCAGATTACATCAATGCCAATTACTGTGACGGCTATCGGAAGCACAATGCCTACGTGGCGACCCAAGGT CCGTTGCAGGAGACCTTTGTCGACTTCTGGCGCATGTGTTGGGAACTGAAGACGGCAACCATTGTGATGATGACGCGATT GGAGGAACGAACGCGCATAAAGTGCGATCAGTATTGGCCCACTCGCGGAACGGAAACCTATGGTCAGATCTTTGTTACCA TCACGGAGACACAGGAACTGGCCACCTACAGCATCCGCACCTTCCAGTTGTGCCGGCAGGGCTTTAACGATCGGCGTGAG ATCAAGCAGCTGCAGTTCACAGCCTGGCCAGATCATGGAGTGCCCGATCATCCGGCGCCCTTCCTTCAGTTCTTGCGCCG GTGTCGCGCCCTCACGCCACCGGAATCCGGACCCGTGATTGTTCACTGCTCTGCGGGAGTTGGTCGCACTGGCTGTTATA TCGTAATCGATTCAATGTTGGAACGAATGAAGCACGAGAAGATTATCGACATCTATGGGCATGTTACTTGTTTACGGGCG CAACGGAACTACATGGTGCAGACGGAGGATCAGTACATCTTCATTCATGACGCCATCCTGGAGGCCATCATCTGTGGGGT GACGGAGGTGCCGGCTCGCAATCTACACACCCACCTACAGAAACTATTGATCACGGAGCCCGGCGAGACCATCTCGGGCA TGGAGGTGGAGTTCAAGAAGCTGTCTAACGTCAAGATGGACTCGTCCAAGTTCGTAACGGCCAATCTGCCGTGCAACAAG CACAAGAATCGCCTGGTCCACATTCTGCCGTACGAGTCAAGTCGCGTCTACCTGACCCCCATCCATGGAATCGAGGGAAG CGACTATGTCAACGCCAGCTTCATCGACGGCTATCGTTACCGTTCCGCATACATCGCCGCACAGGGTCCTGTCCAGGATG CCGCTGAGGACTTTTGGCGCATGCTCTGGGAGCACAACTCCACCATTGTGGTCATGCTGACCAAGCTCAAGGAAATGGGA AGGGAAAAGTGCTTCCAGTACTGGCCTCATGAGCGATCCGTACGCTATCAGTATTATGTCGTGGATCCCATTGCTGAGTA CAACATGCCGCAGTATAAGCTGCGTGAATTTAAGGTCACGGATGCCCGAGATGGCTCATCGCGCACCGTCCGCCAGTTCC AGTTCATCGATTGGCCGGAGCAGGGTGTGCCCAAGTCGGGCGAGGGCTTCATCGACTTCATCGGACAGGTGCACAAGACC AAGGAGCAGTTTGGCCAGGATGGACCCATTACCGTGCACTGTTCGGCGGGCGTGGGACGTTCGGGTGTCTTTATCACTCT GAGCATCGTTCTGGAACGAATGCAGTACGAGGGAGTACTGGACGTCTTCCAGACAGTGCGCATACTGCGATCCCAGCGTC CGGCTATGGTACAAACCGAGGATCAATACCACTTCTGCTATCGCGCTGCACTGGAGTACTTGGGCTCATTCGACAATTAT ACAAACTGAGTGCATTTCTCAATGGGATTGCCAGGTCCACAGAGCTAAAATCTCATCGACCCTGCGTTCGTTGCATACTT ATTTCGAACTCTACGCATAATACGCATTTACGATATACACGGATATCATAACTCTAAAGTATTATAGCCAGCTACTGCCC ACACTTACTTAATACACCTATACTTATATACGTGGATATGTTTAGTTGATAAGCGCACCCCCGCCCGCAGGTTATGTTAC CATTACGATTCTTTGGGGGACTCGATCTTATATATTGCGTTCTATTTTATTGATAATGTAAACTAAAGAAGACTGTTACA CGTTTAATTATCAAAACCTTATATATCAACCCACATGCACTTCTATGTACGAGTATGTGCATCAAGATTTGCTCGCTAAT GGAGAAGGATGTCAATCAACTTGTTGTTGTTCGTGGTACTGCCGCCGGAGTGACCATGAATGAGGGGCGCCACTGGAAAC CGATACATATTTGAACTAAGGACTTGGTAGCTATTAGTCTTACCGCCTAAATCGAATCTCGAACGGAAGCAAAGGGTCGA AAGTAGAAGCCATTTTAAGTAAATCGAAAACTTCTGGCGATTTGGCGCAAACTCCAGACGCTATTATAATATATAATACA ATCAATCAAACACTGCTATGTTAATCAGTTTTCATGCCTTGGTTAATGGAAACCAGTATAAACGGTCCGTGCAATTAGTC AAAAGTCTTGCATAGCGCATATTTTATAAGGCAGCTAGATTCTAAAATTAGTTTTCAAACACAACTTACGTTTTGATCTC CCCGCAAAAGATGACGAACCTTATGGACTGATCGATGTCTTGGTAGCTAACAGACAGTTCAAATTTCAAAGACCATTATG ATAGATCACATACGCAACTTCCAGCCAAGCAGCTATTTTAACTTCAACCTCATTCCCTCGACTTCTATTCAGTTCCCTAG TACATTCCTGTATATACCACAGTCGATACCCGAGCTAGCCACTTATGTATGTGTAATTTAGATTGAAAAGCTATGAAAAG TTATTGTACCTAAGGTGTATGAAGACATAGAGAATAACCGAGCGAACAGATTAGAATGATAACTGTAGATATGTACTGTA GCATATAATTTTTACCATAAAATAGAGCGACACACGCGGTTATAAATAAACATAAACATAAAATCGAGACATTGGCAATA GTTCTTAAGGTACATAATAATGTATATAACTGATAAGCTGTTTTGCAAAACGTTTGAATTCGGCCTCGTAACTATACATA AATATATAAAGAGTTAGCAAGCAAATGCTTAAAAGCAAACACTAAATTATTAGCTGAAACACATGTTAAACAACCATAGT CATATGTAAACTAAATATTTATACGAAATAAAGGTATTTGTTATAACTAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.