Comment: 0-22 hr AEL
GTCACACTGCGCATGTGGAACTTTAAGAAAATCTGCAAAACATTGACAAAATTATTAAATTTAATAGCTTAATCGGACGA ATAAGAGTGTATTTATGTTGATTAGTTGTTGTCTCTGAGCCAGTGGGCAATGTACACGTGCTTACTATGCCTAAAACCGA GGAATCAGCGAGATTTATGTGACGAAATTCCGTAAACAAATCAGCGTGCAGCTCGAGAAGAACCTGATATGCGGTAAATT CAATAGTATGCCCCAGTGGCCACGGAGTCCGCCGGAACCACAAAAACCATGTGGCCCAACAGCAACCTAAACGCCGTGCT GCTCATCCTGGCAGTGCTGGCGTGCCCAACTAGCAGCCAACATGTTCCTCTGGCCATGGCCAATTCAACTAGCAACCAAG TGCCTACGGATCCTGGCTTCATGCCGCTGCAGCAGTCGCCGGGAAGCGTCTTTTTCGTGCACCACAACCAGCAGAATGCC ATGCAGCTGCGACATAGCATGGAGGGCAAGCTGCGGAATATTCGTAATACGGAGCTGAGGGTGGCCAAGATACAGGAAAT CTTTGACTCCATGCACTTCAGCAGCGCCAAAGGAGCGTCAAACACTCGTCAAGCTTCGAGCAACGATAGCAACGTGGTCA ATCCCCAGTTGCAGCGGGACTTGCAATTGTTTAGTGAGCGTCTGAGCAAGAAAATCCAGAAAGCCACTTACGTGGTGTTG GAACTGCGCGAACTGCTTCGCTACAACCTGACCAAGGTCTGGCAATACAGCTACGACGACGAGGATGATGAGTCGGAGAG CGAAATGGATCTAGAAATGGAGCTGGAAGATCTACATGCCAGGAACACGGCCTCACCAACCGATGAGCATGTGCAGCTCT ACTTGAACACCCAAATAGAGAGCTGCCAGCCTGACTACGAAACGGACTTGGAGTACGGCTCCTCATCGACCCAATCGATT CACTACAACAGACAGCAGATCCAAATCCTAAACTATCTCAAGTCGGATGATGCACGCGCTACCTTCCTGAATGAGAATAA TGCCCGTTCCTTGGCTGTCAATGGCTACATATCGAATCAACTAGTACTACTCAAGCGCAGGCTCAGTCGAAGCGATGCGG AAAACTCGAATAGCAAGCCAATTAGCGGGAATCACTTTAAACACATATATTTCCTATCCAATTCGGATGGAGCCTCTGCT AGCCTCCATTTTCGTCAGCTCTACGTGTCAGCTATTAAGCAGAAATTTGTGCTCTTCCTCATCGACGTGGGATCAGCTCT AAGCGCGGAGTTATTCGACCTCTCAAAAAGCTTTGTTCACGAAATGCTCCAGCTGCTGGAAGATACGGATAAAGTTTCAC TGGTGACGGTTGCGAGTGAAGCGAACTTCATGTCCCTAGAAGCCTTTCCCGCCGAGGCTGGTCACGGCATCTACAGTGCG ACTCGTGCGCACAAGGAGGAAATTATAAGCTTCATTAACAGCTTGAGCCGGGCACAGGCCTTTACAAACCACTCCTTGGG ATTTGAGTACTCCTTTGAGCTACTCCATCGACTGCAACAGTCGGGTATGATAAATACGGCAGAGCAACCAGTGGAATTCG TTTACATTACTCGTGGTCTGCTGACCAACTTGTCTGATGCAATGGCAGTACTGCGAGTAGTGGCCGATGGTCAAAGACGA CTCAGGGCTCCAGTGATCATTAACACATGCGCAGTGGTGCTCGACGAGAAGCGTATAATGTACGAAAAACAATTCCTTAA CGACGTCGCCAGTCAGAACTACACCAAGTACGAGATTGATGTGGCAAGTTGGTGGCCCAGTGGACAGGAAGCGTCTGAGT TGGTTGGACGCTTCTATGTGCTCAGCAAAATGCACGCGGAAACGTCGTTACCCCAAACGAGTTCACGGATATTTGGACCG CTCTTTCAAGAGCGATATTTGAGTGATACGCTGGAAGTTCATCCTCCAGTTGTGGACGCAGACAGCGGAGATGTTCTTGT CTCCATCACGCATGCCGTTCCTCCCTACGGTGTTGTAGGAGTAAACTTGTACCTGTCGGATCTGCTTGAGGATCTACTTA ACTACCCCAGTACTACATCCAGTGCCAAGCAGGGCTTAGGGTATGCCTTTCTGCTCGATCGCTCGACGGGAAACACGCTA GCTCATCCGGCTTTTCCACGACCGCTCATTCAGCGCGAGACCTCGTATCCCGTAAACATCGCCTATCTGGAAAACGCCAC AGATTTTTCCAGCCATATACGGGATCGCCTTCTGCGCGAGGAGAGCGGTAATGTCACCACGGATGTCTATGTGGGCAGAC AGCGACTGCAGCGTACCTACTACTGGCAATCCGTGCTGGGATTTTATGTACTCTGCCTGGTAAGCAGTGGCGGCGACACA CTGCGCAATGTTTCCTCCACGCAACGTTACAACCTAAAGGACACCGTGTCCAACTACGAGCCCGGCTACTATGGTGAGAG CATGGACCTACTCTACCATCGCCTGGATTTAAACCAAGGCGGCAGTGCACCGCCAAAAACCTGTCGCTACTTCAGACAAG TAGCCACAATGGATGCTCCAACTCTGTTCCTGAGTGCTGCCGCATTCGAGTCTCCGTTCGGATTCCTTCACAACAATAGG CCTCGCACCCAGCTGCGGCATGTGGAGTCTATAATGGCCTATCTTCGCGACTCAAGTGGCCTGCTGGCCAATATGGGCCT GCGTCCCAGTATTCGGCACGAAGTGGGCGTCCTTTACCAGGCGATGCAGCAGTTGAGACGACGGCACCAGGACGCAAGGG GCTCACTACGCAGCCATGTCATTCGACGGTACATCGCCAGTGTGAGTGGAGTGCTGCAATTGTACCCAGGTTGCTTGCTT AGCAGCAGCTATGATCCAACGCGAAGGCCATGGTTCCGACAGGCAATAGCGCAGCCTGGAAAGATTGTTAGCACAGCTCC GTATCTGGATGCCGGAGGAGCTGGATACATAATCACCATTGCGCACACCATCTTCGAAGGCAAGGCCCATGCGCTGCACT CTGCCCAGCAGGACAGACCCGTAGCAGTGGTAGCCCTGGACGTTCCTTATGCCTTCTACTATCGCCTGATCCTGGAGGGC ACACCGATCTGCCAGCTGCCGCATATAAAGTGTCTGCTGTTCGAGCACGAGGGATATTTGTTGGCTCACCCCAGCATGTT GCAGCCCGCCACACTCACGAAAAACCAGCGACGTCCACATGAGCACCTCACACACAAGGAATCGTATTTGGCCAACGATA TGTTGAACCACGGCCAATTGGTACGAAAACTCGGCTGTGCCAGCTACCAAAACCGCACGTTGCAGCGTTACTACGCCTTC AACACATCTCTGTCCAGCATCCTGGGCAACGTAGTGCACGGCGAGCGAACCAAGTACGCGATTGCCCTGATCCGTGGTAG CAATCTATTCGCAGCCGTACTAAACTCCAGCTGCGATGGCGGCGCCTTTTGCCCGTGCAGCACAATCGACAGAGAGTGTC TCAACTGCAAGCGAATGGACCAAACAGACTGCGAGTGTCCTTGCGAGTGTCCAATGGTGGGAGACAGCAGCTCACCATCG TCCCTCATGTACTTTGCCAACTACACGCGACAATTTCCGTACTGTCCACCGCCAAGTGAACATTTCATTGCACTTCCACC GACCACACAGCTGCTGAGTACTCTGCCGAATTGTCCTGGTTCTGCTGGCATATGCGAAACGTATTCCACTCAAAGAGAAT GCCTCGGAGTGATGGGCTGCGAATGGTGCCAGCTGGATGTGGACGGCAATAGTTTTTCGACCTCCTTCTGCTCGTCCCAG GCGAGCTGCTTCAACGGAGTACTTGCCTCGCTGACACCCTACGGTGAGCTGGACGAGATGGAACTGCTGGCCGCCCACAA TCCGCAGAGAGAGCAGCACGCTTACTCTGCATTTGGTCCGCTAGGAGGAGCCATTGTGGTGCTTGTCATGGTGATTGGCT TTGCCATCTACTGCTACAGACACAATCTGGATGCGCAGACGCAGGAGCACTTCTATGTGGATTCGGTACAGGAAGAGAAT TACGGCTTGCCGCTGTCCAGGTTCAACTTTGACGATTGCAAAGCGCATGACGAGCCGCCTCTTGGTGGCGGCTATGACCA CGCATCTGCACAGCGGCAGCTGATGCACGCGGCGGATATTTCGCCCTACCACGTTTCCAGCGGCAGTAGCTATCGCCGGC CACCCAACGGTGAGTCGGATCATGGCTACAGCACAATGACGCCGCACGAGGACAGTTCCGATCAGCAGTGCTTCACGCTG GCGGAACCTTTGCTGCTGCACGACAAGCGGCACAGCAAGTCGGACACCATGTCCATATCCACATCCATATCGAGTCCCAC GAATCGACAGCAGTCCTCCTCGCAGCCAAACACGCATCCCTACTTGAGCAATCAGCCGACCTCAAAGACGGAACGCTACA AGCAGGTGCAGGCCACGCCCTCGCCATGTCGCGGAACGCCAGCAGGTGGTAGTGTCTACGGGCAGACGACGTTGCCCCTC GAGGGGGATAAGACCCGGCCACACTATATCCTGGCACCAGTGACGGTGCACCGGCACATGGAAACTGCCGAATCGTAGTC CACAATAGCGTTGTTAAGTTTTAGAGCGTAAGATGTCCGCTTGTTTGCCAATTTAGTTATGTAAGTTTCCAAAATGGGCA GTTCAACTGCCGACAGTCCAGAATGCTGGGCCCGCATTCCAAACCCGCAATGTGTTTTGTGAGCTCGCGGTGGCCTCCAT TGTTGGCCCATCTGTATCTATTAGAGCCTCCGTGGACGAGACGAACACCAACTATTAAGTGATGACATTGTTTCTGCCAT TTTTGATCGATGTATTGTATTTGTATTGTATTGTATTTTACTGTAATTTATTGCAAAAAGTATTACTATATTATGTGCAG CTATATACTTCCGATATTTTTAGCATTTATTTGATAAGCACCAACAACAGAAATGTACTTACTTGTCTGATTGTAAGATA CCATGTCGTATATCGTTTTTTGTAATAGCCCCTAAGACTACAGAGTTACTAACTACTGATTTTAGTAATACACATCTATA ATAGCATTGTACGTTTCAAATAAAATGTTTAAATTTTGCAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
