Comment: 0-22 hr AEL
TGAAGCGGCTGCTTTGCAAGGGCAACGTGAACCTGAACGACGCCGCCGCCTCCACGGATGATGGCGAGTCCCTGCTCTCA ATGGCCTGCTCTGCGGGCTACTACGAATTGGCTCAGGTTCTCCTGGCCATGTCTGCCGCCCAGGTGGAGGACAAAGGGCA AAAGGACTCAACGCCTTTAATGGAAGCTGCATCCGCCGGTCATTTGGACATCGTCAAACTGCTGCTCAACCACAACGCCG ATGTGAACGCCCACTGTGCCACGGGCAACACCCCGCTCATGTTTGCTTGCGCCGGTGGTCAGGTGGACGTGGTGAAGGTG CTGCTCAAGCACGGCGCCAACGTCGAGGAGCAGAACGAGAACGGACACACTCCCTTGATGGAAGCAGCTTCCGCCGGCCA CGTGGAGGTAGCCAAGGTGCTGCTTGAACATGGAGCCGGCATCAACACCCACTCAAATGAGTTCAAGGAGAGCGCCCTCA CTCTAGCCTGCTACAAGGGTCACCTGGATATGGTGCGATTCCTGCTTCAGGCAGGTGCAGATCAGGAGCACAAAACCGAC GAAATGCACACTGCCCTAATGGAGGCTTCAATGGACGGCCATGTAGAGGTTGCTCGACTGCTGCTGGACTCCGGTGCCCA GGTGAACATGCCCACGGACTCTTTCGAGTCCCCGCTAACGTTGGCGGCTTGCGGTGGTCATGTGGAGTTGGCAACACTCT TGATCGAGAGGGGAGCCAACATCGAGGAGGTGAACGACGAGGGCTACACCCCGCTCATGGAGGCCGCTCGCGAGGGACAC GAGGAGATGGTAGCCCTCTTGCTCAGCAAGGGTGCAAACATCAATGCCACGACCGAGGAGACCCAGGAGACAGCTTTGAC GCTGGCCTGCTGCGGTGGCTTCATGGAGGTGGCTGCATTCCTGATCAAGGAGGGAGCTAATCTTGAGCTGGGTGCTTCCA CGCCCCTAATGGAAGCCTCTCAGGAGGGACACACCGATTTGGTAAGCTTCCTGCTGAAGAAGAAGGCAAATGTTCATGCA GAGACCCAGACGGGGGATACTGCCTTGACGCATGCCTGTGAGAACGGACACACAGATGCGGCCGGTGTGCTGCTATCGTA TGGAGCTGAACTAGAGCACGAGTCCGAGGGTGGGCGAACGCCACTAATGAAAGCCTGTCGTGCCGGACACCTGTGCACTG TCAAGTTCCTCATTCAAAAGGGCGCTAATGTCAACAAACAGACCACCAGTAATGACCACACTGCCTTGTCGTTAGCCTGT GCCGGCGGTCATCAGTCTGTGGTGGAGCTGCTATTAAAAAACAACGCCGACCCGTTCCACAAGCTGAAGGACAACAGCAC CATGTTAATTGAAGCCTCCAAGGGTGGACACACTCGTGTGGTCGAACTGCTTTTCCGCTATCCGAACATTTCGCCTACGG AAAACGCAGCGTCTGCGAATGTTACCCAGGCAGCACCAACCAGCAACCAACCTGGTCCAAATCAGATGCGTCAAAAGATC ATGAAGCAGCAGCTTCAGCATCAGTTGCAGCAGCTGAACGCTCCCCCTGGCTTGCATGAGTTGTCTGAGGCGGCACGTGC ATCCAATCAACAACATTTCCACCAGCAACAGTTCAGCAGTGCCGGCAACGGATCCTCCAACATCGTGGCAATGGGAACTG GCGACTTTTTGGATGCCGGAGAACTCCAACTTACTGCTACTGCGGGAATGAGTGCGGGAGCCGGAACCAGTACCACGGGC AGTGAGACTGGTATGGAGGAGTATGGCGAAGTCGGAGGAATTGACCTGACTACTCTTGGCGCTCAGCAGCAAGAGGGTCT TATTGCAAAGTCGAGATTGTTCCATTTGCAGCAGCAGCAGCAACAACAGCAGCAGCAGCAACAACAGCAGCAGCAGCAGC AACAACAACAACAGCAGCAGCAACAACAACAACAGCAGCCACCTGCAGCTGGCCAGCACCAGTTAGTGCCATGTAAGCAC TTCGACCTGGACATGGAGCACATCAATTCTCTGCAGCCGCCGCAAAAGGCACCGCCCGCTCCACCTGTACTCTTCCACAC CGTTTGCCAGCAGCCTGTAATGCAACAGCAGCAGCAGCAGCTTCAGCCAGGTCAGCTCAAGTTGAAGGCCATGCTACCCA ACCGCAATCGCGCGTTGAAAACCGCCGAGGTAGTGGAGTTTATTGACTGCCCGGTGGATCAACAACAGCCTGGCGAGCAG GTGCGCACGCAGCCTTTGGGTGAGGATGGAAAGACTCCTCAGTTTGCATGCGCTGGAGAGGATCCACGGTTGCAGCGCCG TCGCGGCTTTATGCCGGAGCTGAAGAAGGGTGAACTGCCGCCGGAGAGCAGCAGCAGTGACCCAAACGAGCTAGCCCTTA AAGGAGCCGACAACAATCAGCCCGTACCGACAGCACTGGACAATAGCGCATGCGCCCAGATTCCAGCGCGAAACTCTGGC GGAGCAATAACCCATTCCTCCGAAGTTCTGCAGAGCACAGCTATCAGCGACAGGCCAAAGGTAAAGGCAACCAACAAGAA CAACCGAAAGCAAGCGGCCGCAGCAGCAGCAGCTGCAGCAGCAGCGGCGGCGGCAGCAGCAGCGGCCGCCCAACACGCCC AGCAAGTGTTGCCGAACCCAATGGTCTCCATCTATAATAACCTGCATTTGCAGCACTTGCAGCATCCGCATCTCCAGTTT CAGCAGCAACTTCAACTGCATCACCAGCGAGTAGCTGGACTGGACAATGCAGCGGCTGCAGCAGCAGCAGCGGCTTCATC CGCGAACATGGCCTACTCTATTTCTCCGGCATCTCCACTTCCCTCGCCCACTGGCAGCGGCAACTATGTCGATCAGCAGC TGCAACAGCAGTCCATGGATGTTGCTCTACAGCGCAAGACGGCCATGGACGATTTCCGTGGCATGTTGGAGACGGCGGTA AATGGTCCAAGGGGCAGAAAAGACCTGGCTCTTAACACACCACAGCTGAACTTCTTCAAGGACGGCTGGCATATGGTGGG AGTGCACAATTTCTTTGGTGATCAGCCAAAGTCGCCCACTGAAACACCGCCTGAAATGGAGGAGACTACCATGTCCTCAC CGACCGAAGCAGATCGTCTCGGATCGGAGCCTCGGGCCGAGATGAAGAACTTGGCCACGCTCTGCTCGGCCGCAGCAGCA GCTGCTGCTGTGGCAGCGGTTAACAAGGATCAGGTTGAGATTAGTTCGGATCTTGAGAGCGAATGCGAGGATGATGCAGA AGGTGGTGCTGGGGCAGATTGCGAGGAAAACACACTGCCGCCGGAGCCAATTGAATTGGCGGCCGCTCTAAGGGAGGATG GCATAATTGTGGAGGAAGAAGAGGATGACGAGGAGGAGGATGATGATGATGAAGAGCAGGATACCAACAGCGGTGAGGTC GACAAGCTAAACTATGATGACGAAGACGCGGAGGTGGACAACGATGGTGAAGTAGACTACATCGACGAAGACGAAGGTGG TGGAGAAGGCGAAGAAGAAGAAGATGATGCAGATGATGACGAGTTCTTCTTGGACGAGCCTGATAGCGACCAAGGAACTG GCAACAATAATAACAATTCCAAAAGCGGCGCCAGTTCGTTGCCATTGAAACAGCGCAAAATGGCCACTCGGTTGGAAAAC CTAATCCTAAACTCGCAGACAGTGTGCGACTTCCCGCCTGAACTTAGCAACTCGGAACTGGTTCATGTCCTGCCCCAAAT AAGCAATCTCAAGGCAGCGGCCAACAGCAACGCGGCTCTGAACAGCGTACTCCAGCAGCAGTTGGCAGCAGCCTCCGCGG CAGCGGCGCACGCCAAAGCGTCTGTAGTCCACCAGAAGCAGCAGCATGGAGAGGGAGATCAGCAATGCGAAGATGACGGC AGTGCCAGCGCAAGTGAGCTATATTCGGGTTTGGAGCACTTTGCCAATGATGGCGAAATGGAGGATATATTCCAGGAATT GGCAAGTAGCCTAAACTATCCCGAGCTTGCCGAGTTTAGCCTCAATCAGATGTGCAAGGGTCGATTTGCTGGAAATTGGG CGCAATCTTCCGGCAAGTGGACTGGTCAGGAGCAGTTGGTGGGTGTGGTAAGGTCGCCGGGTCTCATTAACCCAGGCGAT GTTCCGCAGGATGCCCAGCGACAGGCGAATCTTGTCCTCCTCGACTATCCCATGCAGCAAAACATCCAACTAGAGCAGCG GCTACTCGATGCTGAGGAAATGCACCTGCAGCAACACCAGCAAACACCGCTCTCCTTACTGCCCTTTACGGATGAGCAGC AGCAGCAGCTTCATCACCAAGCTTTGTCCAATGCATCCGATTTTCAGCAACACCAACAGCTTGCCCTGGAAAACGATCCA GAACTAAAGCAGCAGCTTCAGCAGAACTCCAACGCGCGCATAATTAAAGCTGTTGCTGCCCAGCATCAGCAGCAGCCTCC AACCAACTTCGTTTACAACGTGGAAAGCGGCGACAAGAATGCTCCGCCAGTGCAATTGCTCTTCCAGTTGCCACCACACA TGGCGCAACATCAGGCGCAGCAACAGCAGGGCGTTGGAGAGCCTCTTACCGAACAGCAACAGCAGCAGTTACACGCTGAG CAGGCGCATCTCTTTCAGCATCGAACTGGCGGTCAGCGTCCGCCCACCCAGAGTGAGTTAGAGCAGGTGGCTCAAGAGCT ATTGCTTCAGCGAAGCGGCCAGGTGCCGGCAGGAGCTCCTGTTGTTGGTGTTCAGGCAATTCCACTCAAGCAAAAACACT TTAACCTGCATCCGCCGCCGTGTCCACCCACCTGTGTCCAGCATCAGGTGGCCACGCAGACGCATCCTGCTTCTGTTGTA GTGCCGCAGCCTGCTGTAGGATATACACAATTCGCTCTTCAGGCCTCTCAGCAACAGCAAATGCAACAAAACGAGCTCTC CATTTGGCCGATGGCCACGCCTACTCCCGCGCCCAGCAGCGGTGTAAGCTCGACCAAGTCGATGCCCGGCGGCATTGCCA AAAAGGCCATTGACAAGCAGTCGCGCAAGGAACGTCGTTGCGTGGTGCGCCAGACACCAGCAGGCATTCAAGAGAACACC AAACTCCATCTACAGCCTCAGGTCGCAACAGCCCAGCAACAATTTCTAGTGCAGAACCAGTTAGCAGTTGCGACCACCGT GAGTTTGGACAAGACTATCGAAATAGATTCAGAGACGGAATCCAACCACGACACGGCGCTAACCTTGGCTTGTGCCGGCG GTCATGAAGAGCTGGTGGAACTGTTGATCAATAGGGGAGCAAACATCGAGCACCGTGATAAGAAGGGATTCACACCGCTT ATACTAGCCGCTACCGCTGGCCACGACAAAGTCGTGGACATTCTGCTCAAGCACAGCGCTGAGTTGGAGGCTCAATCAGA GCGTACGAAGGATACACCGTTGTCCCTGGCATGTTCTGGCGGCCGATACGAGGTGGTGGAACTTCTGCTTAGCGTTGGTG CCAACAAGGAGCACCGCAATGTATCTGATTACACTCCACTGAGCTTGGCAGCCAGTGGGGGCTATGTGAACATCATTAAA CTGTTGCTTAGCCATGGAGCAGAGATCAATTCGCGAACGGGCAGCAAACTGGGCATTTCACCGCTTATGCTAGCCGCTAT GAATGGTCATACGCCGGCGGTTAAGTTGCTTTTAGATCAGGGATCGGACATAAATGCCCAGATCGAGACGAATCGCAATA CGGCCTTGACTTTGGCTTGCTTCCAGGGCAGACACGAGGTCGTAAGTCTGCTGCTTGACCGACGGGCCAATGTGGAGCAT CGAGCTAAGACGGGCCTGACCCCACTCATGGAAGCCGCGTCAGGCGGTTACATAGAGGTTGGTCGCGTTCTGCTGGACAA GGGTGCGGATGTGAATGCTGCTCCGGTGCCGACGTCCAGGGATACGGCTCTAACAATTGCCGCCGACAAGGGCCATCAAA AGTTCGTGGAGCTCCTGCTATCTCGTAATGCCAGCGTAGAGGTAAAAAATAAGAAGGGTAACTCCCCACTCTGGCTGGCT GCCCATGGTGGTCACCTGAGTGTGGTTGAGCTTTTGTACGACCATAATGCTGACATTGACTCACAGGATAATCGACGTGT TTCCTGTCTGATGGCTGCCTTCCGCAAGGGGCACACCAAGATTGTGAAGTGGATGGTGCAGTATGTGTCCCAGTTTCCCT CTGACCAGGAGATGATTCGCTTCATTGGTACCATAAGTGACAAGGAGCTGATAGACAAGTGTTTTGACTGCATGAAGATC CTGCGTAGCGCCAAAGAGGCCCAGGCCGTCAAGGCCAATAAGAATGCTTCGATCCTTTTGGAGGAGCTGGATTTGGAGCG GACTCGCGAAGAGAGCCGCAAAGCTGCCGCCGCCCGTCGTCGTGAGCGCAAGAAGAAGAAGAAGATGGAGAAGAAAGAGG AGAAGCGCCGTCAACAACAGGGCAATGGGCCTGGCGGAGACGATATGCAAGGCGATGACGATGACGCCAGTGACAAGGAT GATGATTCCGACAAGGACGATGAAGACGAGGAGGCAGCGCCGGCCGCCGCCCGCGAGGAGGGAGACTCTGGCATCGATCA GGGCTCGTGCTCCAGCGGAGACACCAAAGGTGCGCGCTTCGGTGGCAGTCAGTCCGCTCAGGCTGCGGAAGCGGCAGCCA ATTCCGTGTCCACCAACAGTCAGGGAAAGAAGAACAAGAAGCAGGCGAAAAACAAGGTGTTGATATCGGTGGAACCAACT CAACCAGTAATCACATCGAACTCCGTCCTCAAGGGCGTCTGTGCGAAGAAGCATCCCGCAGTGGAAGTTGTAAAGCAACC TCCTGCCACACAACAGGCTGCTCCTCTTAAGCGGCAACTCGATGTAAAGAAGGAGGAACCTGCGCTCAAAAAGAAGGAAG AGAAAAACAGCTCGTCCAGCAGCAGCAGCAAGCGTGAGAAAGAGAATCTTGCGCCCAAGGAGGTTGCACTGCCAGCCAAG CAGCAGCCCAGTAGCTCCAGCAAACTGCAGAGCAGCGAGTCTGCGAGCAACATAAACAGCAGCACCGCTACCAACACCAG TAGCGCCAATACTACTCGGAAGGAAGTTGCAAAGCCAGCGTCACAAACTGCGAGTGCCACCACTTTGAATCCTGCAAAGC GCACCGAAGTCGATGGCTGGAAGGAAGTAGTCCGCAAAAGTAGCGCCCAGCAGACCACAGCGGTGGGAGCGAGTGGAGCC CCACTGCCTGTCACAGCCACCAGTTCGGCCACCAGTGTGCAACATCATCCGCACCACCACCTAGCCAACAGCTCCAGCAA CAGCTCAAGCTCCCTGACCACCAGCACTACTACAGCAGCGTCTTCGGTTCCCGAGATGACGTGCAAGAAGGTGCAAGTGC CCGTAAATGCCATCTCCAGGGTTATTGGACGAGGTGGAAGCAACATTAACGCCATTCGGGCCACCACTGGTGCTCACATC GAGGTGGAGAAGCAGGGCAAGAACCAATCAGAGCGTTGCATCACGATCAAGGGCTTAACCGATGCTACAAAACAGGCACA TATGCTCATTTTGGCACTAATCAAGGATCCCGATGTGGACATATTGCAAATGCTGCCCAGGATTAACAGCAGTATTAAGC AGGCGTCTAGTGGCGGAGCAAGCACCCCGATGTCCGTGGGAACTTGGGACAATCGCACCGCTGCCGGTGTGAATGCGTAT ACCTTTTCTTCTGCCGCGTCCACCACCTCCACATCCTCCAGCTCGTCAGCTAGCTCTACTACACCAGCGGGAGCTTCGTA CAGCAATGCGCACAAGCAGCACCAACAGCAGCCGCAGTCAGTGAAGGGCCCAAGTGGACGGTCATCAACGTCGGTCAAGT CTAATGGCAGTAGCACCAAGGTATCGGCTTCGAGTGGATCGGGTTCCCGGAGCGGCAGGGCTGGCAGTAGCTATCTTGCC CAACAGCAGCCTGGTCGCAGCTCTGGAGGTGGCTCTTCAAATGGCGTGATCAAGAGCAAGTCAGAAAGCTCTTCCAAATC CCTGCCAGCTGCACAAAAGAGCAGTACCACTTTGGGTAAATCATCGACTGTGTCACCGGGTGCACAGAATTTCGCAAAGG CAGCGGCTATTGGACAGTCCTCGCCCAAAAAGGCTGAGGGTGGTGCTACATCTGCGGTGGTCACCTCTGCCGGTGGACGC AGCAGTGGCGTGGTGGCTCCATTTGGACGTGGCAAGCCTGTAGCTGGCCAAGGAGGACCTGCAGCAACGGCGGCTTCCAA CGTTGCCCAGCTGGGAAGTGTGAGTGGCAACAGCAACATATTGGCTGGACCAATTGGCACCTTTAATGTAGCGGATGTGG CTGCTGTGAATGCAGCCGCGGCAGCAGGAGCAGCAGCAGCTACCAACAGCAATGTGAAACCCATTGCTCCCATTGCACCG CCCAGTAAGCGAGTTGGATCTCCCACCCAAGTCCAGCAACAGCATCAAACACACCAACAACAACAACAACAACAATAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
