Comment: 0-22 hr AEL
TCGCGTTCAGAGTCAGAGTCGGATTTATCGTCTGGGTCTGGGTCTGGAAAAGATCTTGGATCTTGGTCTCGGCTTTCGGT TTCGCTTGTGTGTCTTGGTTTCGGTTTCGGTTTCAGTTTCGAATTCAGTTCGTTGGCGCGTTCGCACGGATCGAGTCGTG TGCACGGCTAAAGTCACTCAACTAAAAAAAAATATATATACATATATTAAAATATATATACCAACCCATTTTGTGGCCAA GATAGGCTCGTCGCTCTTATATTCGGGTCAATGCTGTGCTCTGCATTGCCAACGTTATGCTAATTACCGAAAGGATACAA ATCATAGCATACATCATCGGTTACAATCTCTTTGGCAAGACTCCGTCGCACCTCTGGTGACAAATTCCACGCCCGGTGAC CAACCCGCCAAATAAGCGCTAAAAATAAAAAGATACATAGCCAGGAGAATAGCTAGCAACACATATATAAATATAACGAA TATATTTATTTAATATATCACACAACAAATTCGCAAAACGTGCCAAATATAATTTAAATCTATTTATTTTGTTTAACAAA GAGCTAATAAGTCTAAAGTAAATATAAAATAGTTAGTCGTTTAATTTAAATCCTCATTAAAAGCCACAACAAAAGGAAGT TGCCGAAGGAGCAGCCGTTGGCTTCATCATGAGGAAGCCAGAAATGCTGATTAGTTTAATTTTATGGTGCCTTTGCGCTT CCAGTCTGACACTCCGTATCCCCGAGGACCTTGAGAATGCCGCCATCAATGCCCTGAGCTCCGAACGTGCCCGTTCCCTG ACCCCTTGTGGATCCTCCGCGGAGGCAGAGGGCAGTGTGGCCGGTGACTATGACGTTTGCCCGCCGAGCAAGTACCGCCA GCCAACAGCTGAGTGCAACAACGTCTCCCACCGAAAGTGGGGTGCCCGGGGAGATATCTTCCAGCGTCTGCTGGCTGCTG ATTATGCCGATGGTGTGAGCCAGCCGAGGACCTCCAAGGGAACCCATGCTCTACCCGATGCCGAGGTGGTGATCGAGCAA CTGCAGCGCCATGTGGAAGGCGAGCTGCGCCACGCGCACATCACTGCCATGCTGCCCGCCTGGGGTCAACTGTTGGCCAA TGACCTAGTCGAGGTAGGACAGCTTCCCATCTCCGGAAAGTGCTGCGAGCGCGACTCCGCCGTCAAGGATCCCAGCGAGC TGCAGCACTGCTTCGTACGGGCGGGACCCGACTGCAAGGAGTACAAGCGATCGGCACCAGGATTCGATTTGGAGGCGTGT CAGAAGCACACTCGCCAGCAGATGAACATTGCCTCGGCCTACATTGATGGCTCTGGACTGTATGGCTCCACGCGCCATGA ATTCGACCAGTTGCGCACCTACATCAGCGGTGGTGTAAAGGTGGAGTCCTGCAAGTACTGTCAGGTGGCTGGAGCCACTG GAGCACTGCATCGAGCTCTGCTTCAGCAGCACAACAACATCGGCGAGCGGCTGTCGCACATCAATCCCGATTGGTCCGAA GAGGACGTATTCCTGGAGGCCAGACGCATCATCACGGCCACCATTCAGCACATCACCTACAACGAGTTCTTGCCTTTGGT CTTGGGACAGGAAACCACTGCCAAGGAGGGTCTGAGACTGACTGCCGAGAAGCACTCGAGCAACTACTCCAGCTCGGTGA GGGGGGGAATCTACAATGAGTTCGCAACCGCCGCCATGCCCGCTTTCTGGAGCATGTATCCACCAGAGATGCTGGCCAAG AAGATGTCCGCCCACGAGCTGCTGTCTATTGCCGCCCTGCAGAAGTCACTGGTTCCGAGTCAAACGAATGCCGAGGGTTG GTCCGAGTTGGCTCTGGCCGTACATCGCGGTCGTGACCACGGAGTGGCATCCTATGTGCATGCCCTTGATCTCTGCGAGC GTCGATTCGCCGACCAAAGCGCTGCCAATGTGAGCTTCGATACTTTAGCCCAGGTTTCCAATATTCCCGAGGAGTACATC ACCAATCTGCGTGACATTTACCAAAACGCTAACGATATTGACTTGTTGGTGGGTGCTCTTCTGGAAGAGCCTGTTGTTGG AGCTCTCTTTGGACCCACAATTAGTTGCTTGCTGAGCTTGCAATTTGAGCAGCTCAAACAGACCGATCGTTTCTGGTACG AGAATGAGATTCCGCCCTCGTCCTTTACTCTGGATCAGCTGAAAAGCATTCGCCAGACGACTCTGTCCGGATTGCTGTGT GGTTCTCATCAAGTGAGCACCGCCCAATCCAAGGCTTTTATTCTGGAGGACAATTATCTGAATTCCATTTTGGACTGCGA CCAGCTGCCCAAGTTTGATCTAAAACCCTGGCAAGTAAACCCTGAAGATGAAGTGCATGTTGAGCACGTTGAGGTCGAAC CCGCGACTAAAGAAGCCATTGCCGAACTGAGTCCCGAACTCATTGAGGCTGCTGTGGAGCGTGCCAAGCAAGAATTGGAG GAACGCAAGCGTTTCGAGTACGAAGTGTGGCGTACAAAGGGTGGCATCAGCGCCCGATCTCCTGACGGCACGGCTGCCTC CTTCAGCAAGGCCAATTTGGCTGCCCTGAACTTGGCCAACTCGTCCCTGATCTTCGAGCTGACCTCCAACGAGATTGTGA AGACCCTCAACCACATAACCAGACGCAAGCGTCAGATCTTCAATCCTAACCAGAATGCATTTAATCGCAACGAACTGACC GATACCCTGCAAACGGTGGACATAAGTGGACTACTTGGTGGTGCCCAGAAGCAACTTGACACATGCCCGGAACCCAGTCA ACAGTGCGATGCGAACTCGCCGTTCCGCACTCTCTCCGGAAGGTGCAACAATCTGCGTAATCCTAACTGGGGCAAGTCAC TGACCACTTTCTCCCGCCTGCTGCCCGCTCAGTATGAAGACGGCATCTCGGCACCCAGATTGACTGGAGTAACTGGCACC GCATTGCCCAATCCCAGAACCATTTCCACTACAATCCATCCTGATATTTCCAACCTGCACACTCGCTATTCCCTGATGGT GATGCAGTTCGCACAGTTTGTCGACCACGATTTGACTTTGACCCCGATCCACAAGGGTTTCCACGAGTCCATCCCAAGTT GCCGACCTTGCAACTCCCGCCAGACGGTGCATCCCGAATGTAATCCCTTCCCAGTGCCCGCTGGCGATTTCTATTATCCC GAAGTGAATGTGACCAGTGGCGAGCGTTTCTGCTTCCCCTCGATGAGATCGTTGCCTGGTCAGCAATCGCTGGGACCCCG TGATCAGATCAATCAGAACACTCACTTCCTGGACGGTTCCATGGTGTATGGCGAGACCACTTGTCTATCAAACAAGCTTC GTGGATTCTCAGGACGCATGAACAGCACCCAAGTGAGGGGCAAGGAACTTTTGCCTCTGGGACCCCATCCCGAGTGCAAG TCCCGCAACGGTCTGTGCTTCCTTGGAGGCGATGATCGTGCTTCCGAGCAGCCAGGTCTGACTGCCATTCACACCGCCTT TCTGCGTGAACACAACCGCATCGTTGAGGGTCTGCGCGGAGTGAATCCTCACTGGAATGGAGAACAGCTCTTCCATCATG CCCGTAAAATTGTCAGTGCCCAAGTGCAGCATATCGTGTTCAACGAGTTCCTGCCCCGCATTCTCAGCTGGAATGCCGTC AATCTGTATGGTCTTAAGCTCCTGCCCCAGGGGTACTACAAGGACTACAACCCATCGTGCTCTCCGATTGTTTTCAACGA GTTTGCGGCAGCTGCCTTCCGTATCGGTCACTCTCTGCTACGTCCCCATATTCCTCGTCTAAGTGTCCAGCACCAGCCTG TGGAGCCGCCACTATTGCTACGTGATGGTTTCTTCCGCATGGATGCCCTGCTGCAGCCTGGAATAATTGACGAAATTCTG CGTGGTCTGGTGGCCACTCCAATGGAGACCCTGGATCAGTTCATTACCGGCGAAGTGACAAACCATTTGTTCGAGGATCG CAAGATTCCCTTCTCTGGCATTGATTTGATCGCCCTGAATATTCAGAGAGCTCGTGATCATGGAATACCCTCGTACAACA ACTACCGAGCCCTTTGCAATCTGAAACGCGCCACCAACTGGAACGACTTGAGCCGCGAGATTCCCACCGAGGTCATCAAC CGGTTCCAGAAGATCTACGCCAGCGTGGATGACATTGATCTGTTCCCTGGTGCCATGACTGAACGTCCGCTGCAAGGCGG TCTGGTTGGACCCACTTTGGCCTGCATCATTGGCATCCAGTTCAGGCAGCTGCGCAAATGCGATCGCTTCTGGTACGAGA ATCAGAACCCAGAGGTCAAGTTCACGGAGGCTCAGCTGGCTGAGGTCCGTAAGGTGACGCTGGCCAAGATCGTGTGCGAG AACTTGGAGATCACAGGAGATATGCAGCGTGCTGCCTTTGATTTGCCTAGCAATTTCCTTAACCCACGTGTGCCCTGCGC CTCGATGCCCCAGATTGACCTGAATGCCTGGCGCGAAAATGTCCAGGGTTGTCAGATTGGCAACCGCAATGTCCGAGTTG GAGAATCTGCCTTCCCATCACCCTGCACCAGTTGTGTCTGCTCCGCCGAAGGAGCTCAGTGTGCGTCTCTGCGCATCACG GATTGCGGCCAGTTGATCAGGCAGTGGCCCAAAGAGGCTATTCTCCGTGACGAGGTGTGCAACTCGCAGTGCGGCATTTA TTTGACTGGTCAGCAGGCCAGTGGCTTCAATCCCCAACAGCGACAGGGTCAGGCTCAGCCACGTGTGACCCGATCCCGCA ATCAGAATCTCTTCAAGTTCCCCGACCTTACGCCCTTCATTGCTTCCCTGTAAGCTGGAGGGCACTCCACCCTGCAAAGT GGAGGACGGAGTGAATCATCAACTTTGGGTGCGGAGTAAGCAGAAGCTACAAATTTTGAGACGCATTTTGTAAATAAATC TTGCGTTTGTACATAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
