Comment: 0-22 hr AEL
GATGGCACGCAAAGAGTAGATATCACTGCAAATCGGACACAAATCTGCATCCCCCGTCGTTGGACTGCGCGATTTAGCAT CCGGAGGCTGCGGCTAGCCCGCTGCGCTACACCCGCCGCACCACCCCGCCCTCACTTCGCCCACAAATGGAGGAGTCGGG TCCGCCAAGCAATCCGTCTCCGGTTGCGTCACGTGGCCGCGGACGTGGTCGCCCGCCGAAAGTGGCGCTTTCCGCGTTGG GAAATACGCCCCCGCACATTAATCCATCGTTAAAACACGCCGATGCAGAGGCATCGCCCACGGCGCCGGAGGATCAGGAT TCCGGACAGAGCGAGTGCCGGCGCAGTTCGCGCAAGAAAATCATCAAGTTCGATGTCCGGGATTTGCTAAACAAGAACCG CAAGGCGCACAAAATCCAGATCGAGGCACGGATCGACTCGAATCCCAGTACCGGCCACTCCCAGTCAGGCACAACTGCAG CATCCACATCCATGTCAACAGCAACAGCATCAGCCGCATCTGCATCCTCAGCCGCGACCGTGAGCCGTTTGTTTTCCATG TTCGAGATGAGCCACCAATCATTGCCGCCACCACCACCGCCGCCAACCGCCCTCGAGATCTTCGCCAAGCCAAGACCCAC ACAGAGTCTGATTGTGGCCCAGGTTACCAGTGAACCATCGGCTGTTGGTGGCGCACATCCTGTGCAGACGATGGCCGGCC TGCCGCCGGTGACACCTCGCAAGCGGGGTCGTCCCCGTAAGAGTCAACTAGCGGATGCGGCCATTATTCCAACTGTCATT GTGCCCAGCTGCTCCGATTCCGATACGAACTCCACCAGCACGACCACTTCCAACATGAGCAGTGACAGCGGCGAGCTTCC TGGCTTTCCCATACAGAAACCCAAGTCCAAGTTGCGGGTCTCACTCAAACGCTTGAAACTGGGCGGCCGGCTGGAGTCCT CCGATTCGGGGAACTCGCCATCGTCCTCTTCGCCGGAGGTTGAGCCACCAGCACTCCAGGATGAGAACGCCATGGACGAA CGGCCAAAGCAGGAGCAGAACCTGAGCCGAATGGTCGATGCCGAGGAAAACTCCGATTCCGACTCACAGATCATATTCAT TGAGATCGAAACGGAGAGCCCCAAGGGAGAGGAGGAGCAGGAAGAGGGACGGCCAGTCGAGGTGGAACCACAAGATTTGA TCGACATCGACATGGAGCTGGCCAAGCAGGAACCAACTCCCGACCCGGAAGAAGATCTAGATGAGATTATGGTGGAGGTG CTCAGTGGGCCACCCAGTCTCTGGTCAGCGGACGATGAAGCCGAGGAAGAGGAGGATGCGACAGTTCAGAGAGCCACTCC ACCGGGAAAGGAGCCTGCAGCGGATTCCTGCTCTTCAGCTCCACGGCGCAGCAGGCGGTCAGCGCCGTTGAGTGGCAGCA GTCGCCAGGGGAAGACTCTCGAGGAGACCTTCGCCGAGATAGCCGCTGAGAGCAGCAAGCAGATCCTGGAGGCGGAGGAA TCACAGGATCAGGAGGAGCAGCACATACTGATAGATCTCATCGAAGACACGCTAAAGTCAGACAATATTGCTGCAAGCTT GAACAAAGACATTTTCGAGCCAAAAGTGGAAACTAAAGCTACGTGTGGCGAAGTGGTCCCACGGCCAGAAATGGTCACCG AAGATGTTTATATTACAGAAGGAATTGCTGCAACTCTTGAAAAATCAGCCGTAGTAACAAAACCAACTACAGAAATGATT GCAGAAACAAAACTATCCGATGAAGTCGTAATAGAGCCGCCACTAAAAGATGAATCCGATCCAAAGCAAACGGAGGTAGA ACTGCCCGAATCGAAGCCAGCAGTAAACATTCCAAAGTCCGAGCGAATTTTATCCGCCGAAGTGGAAACGACGTCGTCTC CACTTGTGCCGCCAGAATGTTGCACCCTGGAAAGTGTAAGTGGACCGGTACTATTAGAAACATCATTGTCAACGGAAGAA AAGTCGAACGAAAATGTAGAAACCACTCCATTGAAGACTGAGGCAGCGAAAGAGGATAGTCCACCGGCCGCTCCGGAAGA AGAGGCATCGAATAGTTCGGAAGAACCTAATTTTTTGCTAGAAGATTACGAAAGCAACCAGGAACAAGTAGCTGAAGACG AAATGATGAAGTGCAACAATCAAAAGGGGCAAAAGCAAACACCGTTGCCAGAGATGAAGGAGCCCGAGAAACCCGTCGCC GAAACTGTGTCCAAAAAGGAGAAGGCAATGGAAAATCCGGCCAGGTCTTCGCCAGCAATTGTGGATAAAAAAGTACGCGC AGGTGAAATGGAAAAGAAGGTCGTAAAATCCACGAAAGGGACTGTTCCAGAAAAGAAAATGGATTCGAAGAAATCTTGTG CAGCCGTAACTCCTGCTAAGCAAAAAGAGAGTGGAAAATCTGCAAAGGAAGCTATTTTGAAGAAGGAAACTGAAAAGGAG AAGTCTTCAGCAAAATTAGATTCGTCATCACCTAATACACTGGATAAAAAGGGAAAAGATACTGCCCAATGGTCACCTCA GCTCCAAACTTTGCCAAAGAGCAGTACTAAGCCACCACAAGAATCTGCACCGTCAGTGATCTCGAAAACCACATCGAATC AACCCGCACCCAAAGAGGAACAACATGCAGCAAAGAAAGGTCTTTCCGACAATTCGCCACCATCGGTTCTCAAGGCCAAA GAGAAAGCTGTGTCCGGTTTCGTTGAGTGCGATGCCATGTTTAAGGCAATGGATTTGGCAAACGCCCAGTTGCGTCTCGA TGAGAAGAACAAGAAAAAGCTGAAGAAGGTGCCGACCAAGGTAGAGGCACCGCCAAAGGTAGAACCACCCACCGCCGTGC CTGTTCCTGGGCAAAAAAAGTCGCTGTCTGGTAAGACCAGCTTGCGCAGGAACACCGTCTACGAAGACTCACCCAATTTG GAACGGAACAGTTCTCCCAGCTCCGATTCCGCTCAAGCAAACACTTCGGCTGGCAAGTTGAAGCCATCGAAGGTCAAAAA GAAGATTAATCCGCGACGCAGCACGATATGTGAGGCAGCCAAGGATCTCAGATCGAGCTCGAGTAGCAGCACACCCACTA GGGAGGTGGCCGCCAGCTCGCCAGTTTCCACCTCATCGGATTCCTCATCGAAGCGAAACGGATCCAAGAGAACCACGTCT GATCTGGACGGTGGCTCCAAGTTGGATCAACGACGGTACACCATCTGCGAGGATCGTCAACCCGAGACCGCCATACCAGT ACCGCTGACCAAGCGCCGCTTCTCGATGCATCCCAAGGCATCTGCGAATCCGCTGCACGATACCCTTCTTCAAACGGCGG GCAAGAAGCGGGGCCGAAAGGAAGGCAAAGAGTCACTGAGCCGCCAGAATTCGCTAGACTCCAGTTCGAGTGCCTCCCAA GGAGCACCGAAAAAGAAGGCCCTAAAGTCTGCAGAAATTCTAAGTGCGGCGCTCTTGGAAACTGAGTCGTCCGAGTCCAC TTCATCGGGAAGCAAAATGAGCCGCTGGGATGTGCAAACCTCGCCGGAACTGGAGGCTGCCAATCCGTTCGGCGACATTG CCAAGTTTATCGAGGACGGCGTGAATCTTCTCAAGCGCGACAAGGTGGATGAAGATCAACGGAAGGAGGGGCAGGATGAA GTGAAGCGAGAAGCCGATCCCGAGGAGGATGAATTCGCGCAACGGGTGGCTAATATGGAGACCCCGGCCACAACGCCTAC CCCATCGCCCACGCAATCGAATCCGGAGGACTCAGCATCCACCACGACTGTTCTCAAGGAGCTTGAGACCGGCGGAGGCG TGCGCCGCTCCCATCGGATCAAGCAGAAGCCGCAGGGACCACGCGCTAGCCAGGGCAGAGGTGTGGCCAGCGTTGCGCTA GCACCAATCAGCATGGACGAGCAGCTGGCCGAGCTGGCCAACATCGAGGCCATCAACGAGCAGTTTCTGCGCAGCGAGGG CCTGAACACTTTCCAGTTGCTAAAGGAAAACTTCTATCGTTGTGCCAGGCAGGTGAGCCAAGAGAATGCCGAAATGCAAT GCGATTGCTTTCTCACTGGCGACGAGGAGGCGCAGGGTCATCTAAGCTGCGGCGCCGGCTGCATCAATCGAATGCTGATG ATCGAGTGCGGACCACTGTGCTCAAATGGCGCGCGGTGCACCAACAAGCGCTTCCAGCAGCACCAGTGCTGGCCATGTCG CGTTTTTCGCACGGAGAAGAAGGGATGCGGCATCACGGCGGAACTGCTAATACCGCCGGGTGAATTCATCATGGAGTATG TGGGCGAGGTGATCGATAGCGAGGAGTTCGAGCGCCGACAGCATCTGTACTCGAAGGATCGCAATCGTCACTACTATTTC ATGGCGCTGCGAGGAGAAGCCGTCATCGACGCCACCTCCAAGGGTAATATCTCGCGGTACATCAACCACAGTTGCGATCC GAATGCCGAGACGCAGAAATGGACGGTCAACGGAGAGCTACGCATTGGTTTCTTCAGCGTGAAGCCTATTCAACCCGGCG AGGAGATTACCTTCGATTACCAGTACCTGAGGTATGGCCGAGATGCGCAGCGTTGCTACTGCGAGGCAGCCAACTGCAGG GGTTGGATTGGAGGTGAACCGGACTCGGATGAGGGCGAGCAACTGGATGAGGAAAGCGACAGCGATGCTGAGATGGACGA GGAGGAGCTGGAAGCCGAGCCGGAGGAGGGCCAGCCGCGCAAGTCAGCCAAGGCAAAGGCGAAGTCGAAGCTCAAGGCGA AGCTACCACTGGCCACCGGTCGCAAGCGCAAGGAGCAGACCAAGCCCAAGGATCGCGAATACAAAGCCGGCCGCTGGCTG AAGCCCTCTGCTACCGGGTCATCATCATCGGCCGAAAAGCCTCCAAAGAAACCAAAGGTCAACAAATTCCAGGCAATGCT CGAGGATCCCGATGTGGTGGAGGAGCTCTCGCTGCTTAGGCGCGGCGGGCTTAAGAACCAACAGGACACGCTGCGCTTCT CGCGTTGCTTGGTTCGTGCCAAGTTGCTCAAGACTCGGCTGGCACTTTTGCGTGTGCTTACCCATGGAGAGCTACCATGT CGCCGGCTTTTCCTGGACTATCACGGCTTGAGATTGCTACATGCCTGGATAAGCGAGAATGGCAATGATGACCAGCTGAG AGAAGCCCTTCTGGACACCCTCGAATCCCTGCCCATCCCGAATCGCACCATGCTAAGTGATAGTCGCGTATACCAGAGTG TCCAACTGTGGAGCAACAGTCTGGAGCAGCAGTTAGCTGTGGTGCCGCAGGAGAAGCAGGCGGCTCTTCACAAACGGATG GTAGCCTTGCTCCAAAAATGGCAAGCTCTGCCGGAGATCTTCCGCATCCCTAAGCGCGAACGAATCGAGCAGATGAAGGA GCACGAACGTGAGGCGGATCGCCAACAAAAGCATGTCCATGCCAGCACCGCTCTGGAGGATCAGCGCGAGCGGGAGAGCA GCAACGATCGCTTCAGGCAGGACCGTTTCCGACGGGACACAACGAGCAGTCGAATAGGCAAACCCATCCGCATGAGCGGA AACAATACCATATGCACGATAACCACTCAGCAGAAGGGCAGCAACGGAGCCCCAGACGGAATGACCAGAAACGATAATCG GCGCAGGTCCGACATTGGACCGCCGTCCGAGCAAAGGCGTACGCTGTCCAAGGAGCTCCGGCGCAGTCTGTTTGAGAGGA AGGTGGCTCTGGATGAGGCTGAGAGGCGCGTGTGCACCGAGGACCGGCTCGAGCACGAGCTGCGCTGCGAGTTCTTTGGC GCTGATATCAACACGGATCCCAAGCAGTTGCCCTTCTATCAAAAAACGGATACTAACGAGTGGTTCAACAGCGACGACGT GCCCGTACCGGCACCGCCGCGCACAGAACTGTTGACGAAGGCGCTGCTATCACCCGATATCGATGTTGGCCAGGGTGCAA CTGACGTCGAGTATAAACTGCCGCCTGGAGTGGATCCCCTGCCGCCCGCTTGGAACTGGCAGGTGACGAGCGACGGTGAC ATCTACTACTACAATCTGCGAGAGCGCATATCGCAATGGGAGCCACCCAGTCCGGAACAGCGCCTTCAGACGCTGCTGGA GGAGAATACCACGCAGCAGCCGCTGCATGAGCTTCAAATCGATCCCGCGGTCCTGGAAAACGAGCTCATTCAGGTCGACA CGGACTACGTGGGCTCGCTCAGTGCCAAGTCCCTGGCACAGTATATTGAGGCCAAGGTGCGGGAGCGACGTGATCTGCGT CGCAGTAAACTGGTCTCCATCCGACTGATTAGTCCACGACGGGATGAGGATCGTTTGTACAATCAGTTGGAGTCCCGGAA GTACAAGGAGAACAAGGAGAAGATCCGGCGACGCAAGGAGCTCTACCGACGACGCAAAATCGAGGTCCTACCGGATGCGG TTGACGAAATTCCTGTTCCTGGCAAGGCACTACCTATTCAGCCCTACTTGTTCTCTTCCGACGAGGAAGAAACTAAAGTC GCAGCGATCGAACAGCCCGCCGCTGAGGAGGAGCAGGATTCACTGAACATGGCACCGAGCACCAGTCATGCCGCCATGGC GGCTCTGGGCAAGGCAGTGGCCCAGCCGACTGGATTGGGGACGGTTGGCAAACGTAAGCTACCAATGCCACCGAGTGTTA CGGTGAAGAAGCATCGCCAGGAACAGAGGAGCAAGAAGGTCAAGAGTTCCCAGAGTCCCCTGACCGCAACCAGTGCACGC GAGGCCCACGAGAAGTTCCGCTTTGAGATAAGCGGGCATGTGGCCAATTTCCTGCGTCCGTATCGCAAGGAGAGCTGCAC GCTGGGTCGGATCACCAGCGATGAGGACTACAAGTTCCTGGTTAATAGGCTAAGCTATCATATTACCACCAAGGAGATGC GGTATTGCGAGGTCAGCGGGAATCCCCTGTCCTGCACGGAGTCTGTGAAGCACAAGTCCTACGACTTCATCAATCAGTAC ATGCGGCAAAAGGGGCCCGTCTACAAGAAGCCGGCGGAAAACGATTAGAAAATCAAGAAATTGTGTTCTTCTGCGGCACC AGAAATCGAACCTGAACCAAATCCCACACGGAACTAGAACTTTAACTTTAGATACGAATTTAGTTAAGCACATTCGACCC CTAGGATGCGATCTACCCCTCGTAGTGGGTAGCGTTTTAAGTGGGGGCAGCCAAATTGTACAGTTAATGAAATGGCGTAA GATGTAAGTGGAAAAATTCACAATACTTTTTGTTTCTTAAAATTGTCTAAAATTTCTAATAAATTCGCATTTTCATTAAA AAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
