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FB2026_02
,
released June 18, 2026
Comment: 0-22 hr AEL
CCTGAGAGCAGGTTACCCTACGCTCTTCGGCGCTGATTTGGAAACGGTGGTGAATCGGGAGTCTAAGAATGCTCCCGGAA GTGCACCTGTTCCCATCGTATTAAGGCGCTGTGTGGAGGAGGTGGAACGGCGTGGTCTTGATATAATCGGGTTGTACCGC CTGTGCGGCTCGGCTACCAAGAAGCGATTGCTACGCGAGGCCTTTGAGCGCAATAGCCGTGCAGTGGAATTGACCCCGGA ACATGTTCCTGACATCAACGTCATCACCGGCGTCCTCAAGGATTACCTTAGGGAGCTGCCCGAGCCGCTGTTCACGCGCT GTCTTTTCCAGATGACGGTGGATGCCTTAGCTGTCTGCCTGCCAGATGACCCAGAGGGCAATGCGAAACTGATGCTTAGC ATACTCGACTGCCTACCGCGGGCGAACAGGGCCACCCTGGTATTCCTTCTTGATCACCTCTCGCTGGTCGTTTCAAACTC GGAGCGGAACAAGATGTCAGCCCAGGCGTTGGCCACCGTGATGGGCCCACCGCTGATGCTGCATTCGGCGAGTGCGCAGC CGGGTGCTGACATCGATCACGCTCAGCCGATCGCGGTGCTTAAGTATCTTCTGCAGATCTGGCCGCAGCCGCAGGCGCAG CATCAGCAGATGGCGCAGCACATGGGCGGCGCTGCGGGGGCAATGATGGGCGGTTTGGTCACCGCCGGGAGCATGAGCAA TATGGCCGGTGTTGCTTCAGGTCGGCGCGGCGAGTCAACAGGGCAGCGTGGAAGCAAAGTCAGTGCGTTGCCAGCGGACA GACAGCAACTTCTACTGCAGCAGCAGGCGCAGCTCATGGCGGCGGGCAATCTTCTGCGCTCGTCCACTTCGGTAACCAAC ATACTCTCCCAAGGCCATCCTCAGCTCTCAGCCACAGCCAACAATCATCTGTATCAATCAGTAGTGGGTCAGTTAGCTCA ATCGCATCGAGCCTTGCAACAAGCGGTGCAACAGCCCTATCAATTGGGGGGCTCAGTGGGCTCAGCAATTCCCGACCCAT CGCCACTGCCACTTCCAGGCACACCCTCCCCCGGCAGTAGCTCAGCATCGACGGGCTCAGGCTCCGGATCGGGTAAAAGC ACGGACACCATCAAGCGCGGTGCTTCGCCTGTTTCCGTTAAGCAAGTCAAGATCGTCGACCAGCCATCCAGCCCATACTC CATTGTGATGAAGAAACCGCCGTTGCAAAAAGATGCGCCCGTTGAAATCACCACCCCCACCACACAGGCTGATACAGAGT CGACCTTGGGATGCAAGGAAAGCAATGGAACAGCTTCTCGAAGGGGAAATGTAGACTTTTATGATACGCATAAGACGCAG GCAAAGAGTGTGGTAAACGAGGAATCCAGCTACAGCTCCAAGTACACTGGCTCGGAGACCAAGAAGATCATCCCTGGCAA CAGTAGCTACACTCCCAGCAAGGCGAATGCTAGTGGCCTTTCTGGCGGCGAAGACTACAAAGCAATGCGTAACAAGTCGA GCGCTACATCCAGCTCCAGTTCATCGCAGGCAACCGTTTTGAGTGCTGGCTCTACCGCCACATCAGCCCCGACCACTTCT TCAGACGATTCGGATGACCTGGTCTCCTACAAGTCATCGGCGTCCACAAATGCCTTGCTGGCCCAATCGCAGGCCATGAC CACCAGCCAGCTGATGTCCAAGTATCTTAAGCGGGAGCCACGAGTACAGTTTACGCCAATCAAGTCACCGGAATCTCCCT CGCCACCGGGTAGCGGCGATGGTCTGCCGAAGGGTACTTACCAACTAGTTACGCCTATCTCGGGATCGTCGAGCAAACCG GGTGCTACAACTGGAGCTATAAGCAAGTATACCACCGGCTCAGTGGAATCATCTATTAATGCGAACAGCCAGAAGTTGTC ATCGCCCTCACGATTGTGCAACAGCAAGGATAGCAATAGCAGGACTGGAACGGCCAGTAGTACGACCCCCGCGACCTCCA TGGTTTCTACAGGTCGGCGTTTGTTTGACAGTCTCGCCTCTTCATCGTCCTCGGAAACGGAGACCAAGACGTACATAGGT GGCACCACTGCAGCCAGTGGAGCCATTACCACCACCATCTACACCAACGACACCAAGAACTCCGGCAGCAGTAGCAGTAA GTCGGGAATAGGAGGAGGGTCTGGAACTGGGTTGGGAGCAGTTTCGGGAGCAAGCTCCGAGACCCGAAGCTTCGGCAGTA CGCTATTTGGCAGTAGTGGCTTAGGTAATGGCAATGGTAGTAGCCACAACCACTCTAGCGCCAGTCCAAGTCCCTTCACC ACCACCAACGGCAACGGTAACCACAATACCATGCATCTGTACGGCACCTTGCCCAAAAACGGAACTTCCACGGGGGCAGC TCTGTTTGGCGGATCGGCCAACAGTTCTTCGTACCACTCTTCGGCGAGTGGAAGTGGAGCCGGAACCGCCAGCAGTAGTG GGGTCAGCTCTATGACTGGCTCCACAAACAGCTACGACTTCTACACGAGCACAAGTTCCACTGTGAGCAGTTCGCGCCCC TTTGCCAATGGAGGCAACAACTATCACACACTGGGAACCTATAGGGCTCAGTACGCCGCCACTAACCCCTTCCTGGACGC ATTTGATGAAAAGCCGGGCAGCAACGGGGGTAATGCCCACGGGGAGGAGAAGCTAGGCGCGGACAAGGGCCACCACAGAG CGGCCGTTATGGCCTTTCAGTCTTCAGGAGACTCGAAAAACGGTAGCGATGAGTACGATGACATCAAGTGAGGTAGGAAA AATGCTATAAATACGGTTAGGGGCAAGGAATATAGATACAACTACGATTATACGCGTACAATTGGCGAAAGTTAAAAAAA TCTCAAAGCTAGTCTTCCTTATTTAATCTTTCTTAGTCTTTCTTAGTTCACCTTCACCTTTTCGGTCTGCGAAAATGGAT CGTTTAAAATATTTGTGGGCATCGAAAAGCGATCAACTGTCAACTGCTCGACTGCAGTGCCCTTGATTAAACTTTATTTC CCACTCTATCAATTTTATCGGAAAACATCAAGGCGATCTTACCCTTTCGCCATTGCATTGCTCGCGTACTATCGTAGTTA TGTAACAACTAATGTCACACAATGCTGAAAGTGTTCTTTTGAAGTATTTACAGTTTACAAATTTACAAAGTACGATCGAT AATTGCATATAACGCCACAATGAGGTCCCTTTAAGCTTTAAAGCTAGCATAATCTACATACAAAAACTAAAATAGTCGCC GAGCTCTGTGTATGTGTTTATAAATACTTGTGAAAGCTTATCCCTGATAGCGTTAAAAGTCTTGAAGCGCTTGACAGTGG TTTGCAGTTTAGCTAACATAAATGCGCATTCAAAAATATTTACGAACAGTATTTTTAATGAACTAAAATATACATTGAAT AAAGTCAACAGCGTATACAATAAATGACACTTAAGAAATACAAATAGAAGAACTTACCCAAAAGGTTGAATGCGCATTTA ATTTTTCAAAACATACTTCAATATCAACTTACACCGTGATTCGTTACTGATATTTCGCAGTGGAAACTGTATATAAGGAG AGCTATTATAATCTACCCACGTAATCTCTTTTGTGGGCGTTTAAATGTGACCAACTCTCTTATCTGCAAAAGCAATTTAC ACGACTATGACAACCTGTCCAATTAAATGTTCTACGACTTTTTGAGCAACATATTTGCCTAAAATGTTTCGCCCCATGCC TCTTTTATAAAACTTAAATATGCGCATGTGATCAAGTAAGATCGATCTCCCGATTAGGGTTTCACATAGCAGACACCACT CGCAAACTGACATGCAAAATACAAATACATGAATTTATTATCGAATCAATGTGCTTAGTTTTTGTATGAAACAAAGTCTG ATATATACACTAATATACAACGGTACAGTATAATATTTCTAAACATTTAATCATAATTATACAAGCGATGAAAATTTAAT ATTAATATGTATTACTAAATAGCAAGTTAAGAGCGCTTTGCGATTAAGAAGTTTACAGCAGGTATGTTTTCAAGGCTTTT TAAAACGTATTAATGACCAACTGTGCTTAACCCCAATTGTATAGGAAATTACATTTAAATGACCCTATAACGATCTCAAT TTTATAAAACATTAAATTTATAATACTTTCGAATGCATATGTTGCGGAACTGTAAATACCCAAAAAGGAATTTACCGATC CCGTAGTCAAGTAATCAGTAATTAAAGCTGGTTATAGTCATAGGCGTTGGAAAACAGCCTACTGCTCTGATTATTTAACT CAAGTTAGACAGAATCATTCTAAGTAACATCCGACTAAAATAGGAAAAAACAGAAAGATTTATGTTAGCTAACAAAAATG TATAATCATTCGTCTTCATGCAAGTACAACTTTCTTCAGCGACTAAATAAAATCAATTAAAGTGAAAAGCCTCACTTATT ACTTCACAGTACATTCATTGCCATAACTAATCGAAGTTATATAACCTTTAATCAATATGGCGGATTATTTGATTTTAACA ATTAGTACTGCTTCTTATAAAGATCGATTGGGAACCCCAAACACAATAAGATTTTAAATAGTAAATTATTCGGTTAAATG TTGCCAGATTTAATTTATAAGAATGGATAGCATATAAAGTGTTAAACATGATTACCAAACCAAAGAGTTTAAAACCACGA AATAGGCAACCATATAGTTGAGCAAATTGATTTTTATATAGTAAATTGTTAATTATTGCATATAGCACGTAGAATTTTTA ACAAAAGTAAAAAGAAATCGGAAGTTCAGGCGGGTTAGTTATTTTGTGGTGGTGTGACTTTCTTGCCGCCATTGCTGCCC AACTTCTTTATTGATTTTACGGTTTTAACTTCATGCATAACACAGAAACATCAATGTTACTAAAGCTGTCGCCTTCGTTT TAAACACAAGTAAGACCTAGTACCCCTATTAGCTCTGCAACCCGGTTTTTTTGTTGGTTTATCCCGAAAGCTTTAATCGT TGAACGACCAGACTTACACGGTTACAAATTCTATAAATATTAATTATATACCATATAATAGTAGCAGAGTTTGTGGAGGA TATTATGACAGCACTTCGGTTTATTTGCGAATTGTTGTGGCTTCATAGTCGTAGTTAGTTAGCATTAGATGTAAAACGTG GCGGAGATTCGAACGTTGTCAGTTGGTGGTGGAAGAAGGAAGTGGAGGCAGCGACTGCAGTTTCATCGGTTTGTGGTTTG CCGCTTGGAGCTTCTTTACTGGGCCCGATTTTGTTCGATATCTATTTGTACTTGTACTTAGAACTCGTAAGCATACACTA TGGAATATATATATCACTGTGTCATATCGTACATTTAAAGCAAATAACGAAAACAGATTGCAGCTGAACGAATTAGCAGA GGAGAGAAAAATTGTTAACGAAAAGTATTCGTGTATCGAAGTATGTATACAAACCTAAATTAATAGCAGTCAAGCAGCAA CTACATAATATAAATAGATAAATACCAAACAAACAAACAAAAACTCGATTGGCGGGAAACCAAGTACTACAAATTGTACA TGCATTTAATCTAGAATAGCTACAACTAACTAAACACTAATTATCTGATACTGAAACCAATCCAACTTTTAGCTGCAAAC GAAAATGGCAAATGAATTGATAAATATGCTAACACATTAGACATTAAATTAGTTAACGGTGAATTAACGGAAACGAACTA ATTTATTGAAATGTATTGCACAATATCGAACACTTAAACATATTAGAGAAAGTAACTAAGCCTACTACAGGTAATATGCA TACGAATAGAATCGGACACATAGGCAAAGTTGGCAGGATGCAGATTCAGATTGTGTTCAAAACCTAATCTCCAAGTAAAA TCGATCGATTCCTCGCAGGTACTTTCGGATAGTGAGATCGGAATCTGTTGTACCACGATTTGGCTAGTTTCGATATATGT AAAGATTAAGTGTGTTTATGTGAGCATTAAATGCCTATACTTTCGTTTAATTTAAAACTAAAAATGCCCTTTCGCACCCG CACCTTTGTATACTTTATTTTTGCCCACCTTAATAACATTTTGACGCTTTACGGGGATTTTTGTCATGGTTTTTCGAGCG GTTTGTAAGGAATGCAATTAAGGAAAAGATTTCTTCTGTCGAACTTAATAAGTAAATGGAGACCAAATGCTCAGGGCTTT AGCAAGTTATAGTAGGCGTAATGTACAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.