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FB2026_01
,
released March 12, 2026
Comment: 0-22 hr AEL
CGTACGTATATATCGTTGCAACTTTTTAACAATCGTGTACAATCTGTATGTTGTATATGTTCAATTCAAAATTCAGCAAC AATAGCAACAACGCAATTCAGCAACAACAACAAACAAAACCGACAAGCTAAACTTGGCTAAGACAAAATCAACATCCAGC GGCAGCGCGGCCATAAACAAAGCCAAAATCAAAAGTCATTCGGATATATACATATATTCGCCCATAATAGCGAATAAACA GTAACAGAACGAGTGGATTTAACGAAACAACAAAAAGAAAAGAAGAACGATAAAATCCAAAGGAAAAATTTATAGCCGTC TGTGCTGTACTAGGATTTTTGTCAATAATAAGAAAGGATACAAAGCCACAATGACTCTAGTAGCAGTTGTACTTGTCAAG TCCTTGGCCCTCGAATCAGGTGAAGCCAGCCGATTTCAATGAGAGGAGCCTGGGCCCTGAGAATCTGGAGCAGGCGAGTG CACAGACATTGCTGAGCATGAAGCAATCACTACGGACCACTACACAATTTTATCCCTTGAACAAGAAGAGATTCTTATCA AAAACCAGAACAGAAGCACCAGGATCAGGAGTTGAAAAAACGATAGGCGGCGGAGGATTACCAGGAGTAGGAGTCGCAAG AACAGCAGGAGAAGGCGTAATCAGTCGCAGGAGGAGGAGGTGGTCACCGGCCACATAAGACCAGTTCCCCCCCCTAAACC CACTTTCACCCCCCCTACATACCCTCTAACGAAGGAATCGCAAGGAGGATCAAATCAGTGGAAAAGGAGGAGAGACGGAG GCGGCCCGTGTGTGTGTGTGTGTGTGTGTAGGTGGAGTGTGTGTGTTGGCCGTCTCAGTCGGATAACCATCAACCCGGAT CCTTAATCCCAAGACACAAATCTCAAACTGAAGCACCAGAACCACCACAAGAACCACCACCACCATGTCGTCCAGTGAGC CACCGCCTCGTTACGAGCCACCCGTGGAGCCAGTCAATGGCATTGTACAGCCACCGGTGATTCCACCAGCGGAGCGGCCC GGCCGCAATACGAACCAATTGCAATATCTGATCAAGACGGTGATGAAGGTGATATGGAAGCACCACTTCTCGTGGCCCTT TCAACAGCCCGTCGATGCCAAGAAGCTCAACCTGCCCGACTACCACAAGATCATCAAACAACCCATGGACATGGGTACGA TCAAGAAGCGGCTGGAGAACAACTACTATTGGTCCGCCAAGGAGACCATACAGGACTTCAATACGATGTTCAACAATTGC TATGTCTACAACAAGCCCGGCGAGGATGTAGTGGTTATGGCCCAGACGCTCGAGAAGGTCTTCCTGCAGAAGATCGAATC GATGCCTAAGGAGGAGCTTGAACTGGAGCCGGTCACGGCCAAGGGTGGCAAAAAGAAGCAACGGGCGCCGGCTACGCCCA AGTCATCGTCGGGTGGCGCTGGAGCATCCACCGGTTCTGGTACATCCTCGGCAGCAGTTACCAGCGGACCAGGTAGTGGC TCCACCAAGGTGTCAGTCGCTGCCTCATCCGCACAACAGTCCGGCCTGCAAGGAGCGACGGGAGCAGGCGGTGGATCTTC AAGTACACCGGGAACTCAGCCGGGTTCCGGTGCGGGAGGAGCGATCGCCGCCCGACCCGTATCCGCCATGGGCGGCACGG TTTCATCGACGGCCGGCGGTGCACCGTCCATACCACCGATTAGCACAATGCCACCGCACACGGTACCCGGCAGCACCAAT ACGACGACGACAGCGATGGCTGGCGGCGTTGGTGGGCCAGGTGCAGCTGGAGCCAATCCCAATGCTGCCGCCCTGATGGC TAGTCTTCTGAATGCGGGCCAAACGGGCGCCTATCCCGGTGCGCCCGGCCAGACGGCGGTCAATAGCTCCTCGCTTCTAG ATGGCAGTACAGCCGCAGTAGCGGCGGCAGCAGCAGCGGCTGCGGCGGCGGCGGCGGCGGCAGGAGGTGCAGCGGGAGCC GCTGGAGGAGCAGGGACAATACCAGCAGTTGCCGTCAATGCCGCTAACGCCGTTCAGGCCTATGTGAATGCGGGCGTGAG CGTCGGAGTGGACGCCGTGATACCGCCTCAGCAGCCCGCCAAAATAAAGAAGGGTGTCAAACGGAAGGCGGACACAACAA CGCCGACGGCCAATGCCTTTGAATCCCCGTACACGCAAATGGACTCCAAATCGGCCAAGATTGCGACGCGGCGGGAGTCG AATCGTCAGGATCTTACATTCCAGGGCTCGGGATACAATATGTCGCCGCTAGGCGTCTCCGGAGTGCCCGGACTTGGCGG TCTAGTTGCCGGCGGCGTGGCTGGTGTTGCGGTGGCCAAGAACAAGGAGAAGCTGTCGGATGCGCTCAAGTCGTGCAACG AAATCCTCAAGGAGCTCTTCTCGAAGAAGCACTCGGGCTATGCTTGGCCATTCTACAAGCCAGTGGACGCGGAAATGCTT GGCCTGCATGACTACCACGACATCATCAAGAAACCAATGGATCTGGGCACAGTCAAGCGGAAAATGGACAATCGCGAGTA CAAGAGCGCGCCGGAATTTGCCGCCGACGTGCGATTAATATTCACCAACTGCTACAAGTACAATCCGCCAGATCATGATG TTGTGGCCATGGGTCGCAAGCTGCAAGACGTATTTGAGATGCGCTATGCCAACATCCCCGATGAGCCGGTAGCCAATGCG GCCCACCATCACGGACATGGCCACGGGCATGGTCATGGCCATGGCCACGGTCACGGGCATGGTCACGGACATGGTCATGG TCACGGATACGGCGGCTCCTCCTCACTCAAGCACGATGCCAGCGATTCGTCCAGCGAGGACTCCAGCGATACCGAAAACG AATCCAACTCGGATGAGGAGCGGAGCGCTAGGCTGAAGATGCTCGAGTCCAAGCTGCTCGGTCTGCAGGAGGAGATCCGT AAGCTGTCAGAGGAGGCCTCCGCCAAAAAGAAGGCGAAGAAAAAACTCAAGGAAAAGAAAAAGTCAATAGGCGGCGGATC AGGCTCTGGTTCGGCTTCGCACCATTGTCACGCCACGGGCGGCGGTGCAAATGCTGGCGGAGCAGGCGGTCCCGGATCAG GCGGCCATGGGAGCGTTTCCGTGCCAGGCGGTGTCGGGTCCTTGGGTCCCGGTGGAGCGGGCGGCGCTAATCTCAACGCG CTGCTCGGTGGCTCATTGGTTGGCCATGGCGGAGCGGCCGTCGCAGGAGGCGTTCCCAATGTGGGTGCGTTGCACAGCCA GGTTCACGACGTGGCCATGGCCTTTAGCCAGATGGCGGGCGGCGGTGCCGCGGCCGGTGCTGGCTTTGGTGCCGGTGTGA CAGCAGCAGGAGCATCGTCGGGCGGCAAGGCGGGCACACTGGCCGGCGCTCTGGCAGCGGGCGCAGCGGCAGGTGCCGGC GGTACGACGGCTGGCAGCGGCAGCAGTAAAGGTGCTAAGAGCAAGGGCGGACGTGGCGCAAAGGGCAGCGGAGCCGGCGG CGTTGGTGCCAGCAATAATGCCGCTGCGGGCAATGCGGCTGGTGGCGCAGCGGGAGCTGCAGCGGGCGCTGGATCTGTTG GAGGTGTTGGCGGTGCAGGAGCAGCGGGCGGCGGCAATGCCTCCAAGCGGGCCAAGGGCAGCAGTTCGGCGGGCGCTGGC GGTGGTGTTGGTGGCGCGAATGCCAGCGCCGGTGGCGCCGGCGCACGCGGCAGCAGCAAGAAGAAGCCTAGCCAGGTGAT GAACTTTGACTCCGAGGAGGAGGACACGGCCAAGCCAATGTCGTACGATGAGAAGCGCCAGTTGTCGCTCGACATCAACA AGTTGCCAGGGGACAAGCTGGGCCGTGTGGTTCACATTATCCAGAATCGGGAGCCATCGCTGCGTGACTCCAATCCCGAT GAAATAGAGATCGACTTCGAGACGCTGAAGCCGTCGACGCTGCGCGAGCTAGAAAGCTATGTGGCGTCGTGTTTGCGCAA AAAAACACGTAAGCCATATTATAAAAAGCCTTCTGGCAAGTCGAAGGACGAGCAGATGGCCGAGAAAAAGCAGGAACTGG AGAAGCGCCTGCAGGACGTCACCGGCCAACTGGGGGCAAGCAAGAAAACCGCCAAGAAAGATGAGTCCGCTTCGAGCAAG GTCGAGGCAGTGCAGCCGGCGAATCCCGTGTCGTCGAGTTCCAGTTCCAGCGATTCATCGTCGTCGAGTTCGAGTGATAG CAGTTCGAGTGACTCGAGCGACAGTGAAGCAGGTTAGGACGCGTTTTGTTTGTTTGTTTATTTTATACAACTTTTTTTTT ATTAGTATACATAGAAATATGCAAATAGAAATATATATATATATATATATATACATATATGTATATATATAGATACGTTG TTTGAAAAACAAACAGCAAACTCACTAAACACACAAAAACCGATGCAGATGCAGATACAAGTGCAGATACAGATGCAAAT ACAGATTGCGACTTTAGATACAAGACCCACAATTTTATTATATTATCGTTTTAAATTAGGTAACTATAGATACAAAAAAA AAAAATGAAAAAAACATAAAGCCAAGGGGCGGGGCGGGCGTGGCTTGAGTCAGAGAAGAGAAGATTCGAACGAGTAATGA ACTGTCGTGAAATTGTAAAAATATGTATTGCAAATAAATATGAATTGAATATAAAACGGACAGAATCAGGAGACTTGAGC AAAGCGATTGGCTTAATTCATTGAGTTGGAAATAAATAGGTTTATCTAGTTGTAATGTAATAGCTACACCATTAGCACGC CCAAGAGCGTATGCCAATACAGATGATCGTGTTTTTTTTTTTTGCCTAACATTTCTTTCTATTATTTTGTTTAAGTTAGA TACGAAAATCAACACACTGAACTACGAAATAGAAGGAGACAGACATAGACACTGAGTCGCGAGCTGGGGAGCGAGCGAGA GGGGAAATGATAAGGGTATTGTTTCGATTGAAGACGAAGACCGACTGACCAAGAAATCCCGCTCAAAAAAAAAAAAAAAA AA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.