Comment: 0-22 hr AEL
TTCACATGCAACTTTCTGCTGAGCGCGTTTTTTTTTGTTATAAAAATCTAAAATACAAGAATTAGATTTAGCGAACTGGC TCCTAGTGTTTAACCAAGTACACCTTTAGTTAGCATTACGGTTACTTTCCCGTGGCGAAGGAATAATTGCAAAACCATTA GCTCTCGACAAAAGTTGTGCGAAGCAAAAAGAGAAGTTCGCGAGAAGAAGACGCCTGTGAGCGTGACGAAGAGAGCCGAG CGAGAGAGTGAAGAATATCGAATCGAACGGAACAGGGCCTCGAAAGAGAACGTGAAGGAAACGGATCGAAAAATCGCAGA CTTTGACAACCGAAGAAAAACCCACATTCAAGACACAAAATTCCGGTTGCCTGAGGGGCAAATGTCAAATTGCGGCGCAT AAATTACATTAATTTAAAGCTCCCCGCACAAGGCTCATCCAAATTTTCACACATAGGCAGCAGTTGTCGCAACAGAAAAA AGGATTCAGCACACGATGTTCGCTGGAGCATATGCACCCAACGCTGGGGGCGTTCAGCAGCACACTGGCGGCGGTTACTA TGGCAATGGTGCCGCAGCAGGCGCTGGCGGTAGCGGCAACGGGGCCCCACGCCACGAGCGTCAATACTATCCGCGCGGCA ATTTCGGCGGTGCCGCCGGGGGAGCTGGCATCAATGGAGCAGCCACTGCGGCTGGAAGCATGCACTTTGGTCAGCCCTAT GGCGTGATGACCTATGGCATCCAGAATGGTCTGGGAATGGGAGCCGCCGGTTCTGGTGGCGGCAATCCGTATCGCCAGCA AAACGGTGTTGCATTCGCCGGTAATGGACTTGGAGGAGGCGCCGGTGGCGGATCCGGAGGATCTGGCGGATATGGAGGAC AGCGTGGCAAAAATCCAACCTACGCACAGCGTTATCAGAAGCCGAACAACGGCGCCGGAGTTGCTGGTGGCTACCAAAGC AATAACTACAATGCTGCCGCCTTAGGCATGCTTTCCAAAGAGGAGCGCGCCGAGATCCAACGTGAAAAGGCCAAAAATCC CGGCCGAAATTTAGTGAAACCCAAGTGGGAGAACCTCGAGCCCTTCCTCAAAGATTTCTACAACATTCATCCGAATACAC TGGCCAAATCAGAGCAGCAGGTGGCCGAGATCCGGCGCGAACTGGAAATCACCGTGTCTGGCAATGAACTACCACACCCG GTGGTCAGTTTCGAGGAGAGCTCGCTGCCCGCTCACGTCATTGAAGAGATGAAGCGCCAGGGATTCACCAAGCCCACTGC TATCCAATCGCAGGGTTGGCCCATTGCCTTAAGTGGCCGCGATTTGGTGGGCATTGCCCAGACCGGTTCAGGCAAGACGC TGGCATACATGCTGCCTGCCATCGTGCACATTGGCAATCAGCCGCCAATAATTCGCGGCGAAGGACCCATTGCTCTAGTG TTGGCCCCCACCCGGGAGCTGGCCCAGCAGATCCAATCTGTCGTGCGAGACTATGGACATCTGTGCAAGCCAGAGATTCG TCACACTTGCATCTTTGGCGGCTCATCGAAGGTGCCGCAGGCACGCGATCTTGACCGCGGCGTCGAGGTGATCATCGCCA CACCAGGACGTCTGATTGATTTCCTTGAGAATCGCAACACTAACTTGCAGAGATGCACTTATCTGGTGCTGGACGAAGCC GACCGCATGCTGGACATGGGTTTCGAGCCGCAGATCCGTAAGATCATTGAACAGATTCGTCCCGACCGTCAGGTGGTCAT GTGGTCCGCAACCTGGCCAAAGGAGGTGCAGGCCCTAGCCGGAGACTTTTTAAACGATTATATCCAAATCAACATTGGTT CGATGAACCTATCGGCTAACCACAACATTCGCCAGATTGTCGAGATCTGTACAGAAATAGAGAAGCCGCAGCGCTTAGTA TGCTTGCTCAATGAGATTTCTCCCATCAAGAATTCGGGAAACAATGGGAACAAAATTATCGTCTTTGTGGAGACCAAGAT CAAGGTGGAGGACATCTTGCAGATCATTCGAGCCGAGGGTTACAACGCCACCTCAATTCACGGCGACAAGACCCAGAATG AGCGTGATTCAGTGCTAAAGGATTTCCGTAACGGCAAGTCCAACATTCTGATTGCCACCGATGTGGCTTCGCGCGGCCTC GATGTCGAGGATCTGCAGTACGTAATTAACTATGATTACCCGAACTCCTCGGAGAACTACGTGCACCGTATCGGGCGCAC TGGTCGCTGCCAGCAGCTGGGAACTGCCTACACATTTTTCACGCCTGACAATGCCAAGCAGGCCCGTGAACTGATCTCTG TGCTCGAGGAGGCTGGCCAGACGCCGTCGCAGGCTCTTTTGGATCTTGCTCGTTCGATGCCCAGCTCGGGCGGCTATCGC GGCAACAAGCGCTGGAACAACAACAGCGGCGGGGATCGCAACACTGGCGGTAACAACGGCATCTATCAGCAGCGCAACAA CAATCCGCTGAATTACCAGGCCGGTAACAACTATAACAACAATCGCAGCGGCCCCCCAACTGGCAGTAGCTACCAGCAGT ATGCCGCTGGCGGCAATACCTATCTGCAGAATGGTGCTGGTGGTGGCGTTAATTGGAATCGCATGAACCAGATGGGACAG GATGGAGGAGCCGGACAACAGCAGCGAAACGGCAACACCTATCGCCCGAGGGCTCCGTTCAACAATGGCGGACCACGCGC CATTATGGGCCATCAGGGTGGCGGCGTCGGAGCTGGTGGAGCAGCCGGCGTAGGCGGTGGCCCACAGCAGCTCATGGCAC CCCAGCAGGGCGGACAGTATATGCCACAAGCATACCGCGGACGTAGCCAGTTTGTGCCGCAGGGAGCTGGTGCCGGTGGC ATGCCGCCACGATTCCAGCAGTATCCCAAGCGGGAGTACCAGCAACAAGGAGTAACCCACTACGGTCAGCAGGCTTTGCA GCATCAGCAACAACAGCAGCAGGCTGGCCAACAGACCAAGCCGCCCAAGGACGAAAGCAACAAGTATGTTCCGTCGGCTG CGGTGACCAGCTCGCTGGTCCAATACACGCCAGCCAATTCGCCAACGATTGCGGCCGTAGCTGCTGCCGCTGCAGCAGCC GCCGGACGAGCTGCAGCTGCAGCTGTGGCTCCGGCTCCCGGTGCCGCCTATATGTACGATGCGGCCGGTGTTCTCACTAC CGCCGCTGCGCCGGGCACCAATCCATATGCCAACCAGTTCAGCATGCCGGCCACCTACTATGCCGCCCAACATCACCAGT TCGCCCAAGCCGTGGCAGGACCCGGACCAGCACCCGGCCAGGCCATCGAGTAGGCGGGACAACAATACGCTAACGCGCTC ATGGGACGAAATGGAAGTGGATCGTGGAAAGCTACAACAACAAAAACAACAAGAACAGCAGCAGCAGCTTTAATAAAATC AACCACATTGCAAGCAACAACAACTCAAACAACTATAACAACACCAACGACATGGACAAATGAATCAACAGTTATCCACA TAACACATATAAAATCCAAATGCATATGATGATAAATGAACGAATGGATATAATTTTGCCAACATAACAGAGCATAGCAG CTCTTTAACTTTTGACCAAAATCGTTGCCTTTTTGTTTCTATATTTATTCACACCTCACTACTAATATTAGTAGAACATT TGTGTTCTCTTTCGCTTATTTAAGCATTCGCAATTTTTTGTTTGCGATATTGACAACTAACCTTAAATTCGTTAATGTTC TCTGGACAAACTGCCGTTAACCATCAATAACAAGGGCCAACGCACGAACATCGCCTTCGAAACCCTTTTATGTTTTCCAA ACACAAGGTAAACCGAATCAAAACTCAAGAAAATCCAAAAAGGGAACGATTTTTGTAGTTTTTAAGCTCTTTCACACACA AATCACACACACACACACCCCGACAGTTGCATGTTCTTCGAATGTTTAAAAAGAATTGGGTAGATTATGTTACCAATGTT ATGTTATATTTTTTCTTAGTATAGCCATTTGGTATATGTTATACTAAGAAAAAAAAAATGATCCAGGGACAGACACTGTT AATTAATTGATTTTCGAAAATTCATGTGTATCAATCTTTACACAAAGCTAAGAACACAAGCACGCAACCAAAAAACACGA AAATATTGTATTCGAAAATTGTCAAAAACAATGAAAGGCATTGAAAGAAAAACCCTTTTCGCTAACTTGGTTGGTATGAA AGGACGGTTTGCTTATAATAACACATATTTAAATACATTTTTAATTATTTAAATATATATATATATATATATTTAGATAT ATATATTTATATATATTTTTTTTTCCGAGAAAATTTTGCAGCAAACAATTACAACAAAGGGAAAACTAACAAAATTCTAC ACAAATTCCTTTTAAGAAAACGCAGCTAACGGTTGAGATATTTGTATAATGTACAACACACATGTTTTGGCCGAATCCAA AATAAGAGAAAAGGGAAATGGGCAGCGCTGCTTTGGTAGGAAGTATGTTAAATTAAAACTAACAAAACTAAAACAAGTTA CAATCGGTAATAATAAAACGATTATAAAGCAATGGGAGAACACGTACACAGCCACATAGAACCCCCACAAGCACACGCAC GCACACACACATTGACACTCAACACACACACACAACGAACATCACAATGACAAGCAAATTTACAGTTATCATTTAATTTC CCATTTTGATACATATACGTGTGTATTCTATATATGCTATATATATATTTACATCTATATGTAGATTCGAGCTTGATTTT CAATGGAGATTAAAACAAAAACTCATTTTGTTGAAGATTTATAGAATTCGATAATAATCCCCTAACACAAAAATTCGCAT AAATTAACGAGGTTTTTTCAAAAGTTCTATGCAAATACTTGAAAAAAATTCAATTACAATTTTCAATTATTCAAAGACCA AAGAACAGAAGCAGATGAAAATAAGAAGAAAGAAAGCGAAGAAAAAAGAAAAGACGCCACATTCATCCATAAAAACAATT AAAGCGAACACGAAACAAGAGGAAAAGAAGGAGGAACACAAAACAAGGTTAGAAGATCAAATAATGCCAAGCAATGCCAC ACGAAAATAATGATAATAATAATACAGCAAACATAATGCATACTTCTGACTATTCCAAAAGCTCTTTTTTTATTTCCCCA TATCTTATTAATTGATTTGAAAATCATTTTGATTTGCATTATTAATTTCTCTCTCTTCCGAATACTTTTGGCCAGAGTTC AGGACGAGTAAATTAGTAGTAGTAACATTAATTGGCAGCTCCAGTCATATAGTTAGCAGTAATATATCCATATATCGTCT GTTCAAGCAGTTGTCCATGTTGTTGCAAGCAGTGCGTTATCCTCGCCACGGGAAAGCAGTACAAAAATCCGAAGAATTTT GGGAAAACGTAACGGACACAAGGGAATATTCTACAGTGGAATTGTATGAGAACAAGAACACAAGGAAAATATATGAAAAG TCATGTAGATTATATTAGAAATATATTTGTTAGCCGATCGGGGGGTGTGAAAGAAACATTTTTGTTAATTTTTTTTGTAT AAGTTCTCTACGTATACGGTTTCTTTTTAGCAAATAAATAAAAATCGGGGAAAACAAACGGAAATATTTATATATGTAGG GTATATCGTCAACACAAGGATCTCTTTTTAAAGCAAACGAACGGAAGACTCGAATTCTTCTATATAGACCTATAATTCAT AGAGAAGCTCGCTTTTATTTCATTTCATATTGGTAGATTTCTCATACATTTTGAAAATCATTGGAGACGGGATTCGAAAA CTACAACTAAAAAATGTTTTTTTTTTTATCTACTCAAGGGGTATACACAAAGTTACCAGTGAATTAACGTATTTATTTTT ATAATTGCATACTTCGTAATTATGATTTTGATGCAACCGAATTGTGATGATGTGATAGACTTATATAAACAAACGAAAAT ACAAAAATACTAATTAATAACCAATTTAAATGAAGTAATCGCAGTGCCCCCAAAAAGGCCAGGCCAAAAACACTGAAACT GAAGCAGAGGCAGCTGAAACTGAAGCTGAATCTGAATCGAAATCAGTATCTGAATCAGAAGAGGAATATGAATATATGTT ATGTATTTGTATTATTGTCGTTGTCGTTGTCGCTCATAGACAATGTCTGATGGCTAAAATATAATGTATTATGGATGACT TGAGGCATACATTCTTTTTTTGAAGAAATAAAACTATAGAACGCGATATTTGTATTGTATTTATCTATAAGTTGTTGTGT ACACACTGCGAAAAACAAACAAACCAAACAACAAACTAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
