Comment: 0-22 hr AEL
CTGAGGTCCAATTGTTGTGTTTATCGCTTTGTTTAACGCCACTCGGTGGTTCAGTGGTGCAAATATGCAGGCAGCGATGA CTAACTGAGCAAGTTTAGCGGAAGACCAAAGAATCCATCAAAGATTTCGATCGAGATGTCATCTGTGGTGGACTGGGGCG ACTTTGCCTGGAACTGGACGCAGTTCTGTCCGACGGGATTGAAACCGTTCGCCAACGAGACCAACGATCTGCTGCCTTGC TTTCAGGAGATCCTGCTCCAGCTGCCCGTATACACGCTCTTCGCCGCCATATCCGCCTACAATTTCGGCAATCAGTCACG ACATGTCGTACGTAATGGATTGCAATTGCGTATGCTTTCGATTCGTACCTTCCTGGCCATGGTACTGGCCCTGCTACCGG TGGCCAAGGTGTTCGCCTTCCATCAGCAGGGCGTTGAACTGTTTGCGGCGGATCTGCTGGTTGTCAGTGCCGAATGCATT ATGTGGGTGGTTCACAGCGGTTTCCTACTGACAGCCCGTCAATATGGAGAGCTTAGTCATCGGGGATCGCTGCTAATCAA TGTCGTTTGGCTCACAGTAGTCGTTTTGGATGCAGTCTGGCTGCGGACAAGTCGACACTTTAGCTGGTGGCCCTGGAGCT TGGCCACGTTGGTCTGCGATCTTCTGTTTGGAGTCACCTTAATACCAAAAGGAAGTGCTGTTTACTCCAGTTTGCCCAGA TCAGGAGTTTCTGCCAGAGAGGATGAGGGCCTGCTATCCCAGCGATATACATATTTTCAATTCGATCTGAATGAGAGCCA TTTGGGTCATGCCCAGGACGAAGCCAATTTGGGATCGCGTTTCCTCTTCCACTGGGTTCAGCCCCTAATCTTGAAAGGAG TGGCTGGAAAGCTGAGAAAGATTGAGGATCTGTTCGATCTCCCCGATGCCCTGAACATCACACGACTGAGCGAGAAGTTG CACTTGGCTCTATCGCAATCGCAAAGTTTATGGAGAGCCCTGCATAGATGCTTTGGTGTGGAGTTTTACCTTATTGGTAT CCTTCGATTGATCGCCGACTTGAGTGGCTTTGCTGGACCTCTACTGCTCGGAGGACTTCTCCGACAGGATCACGCAGATC CTAATCAAGTATACTATTATGCCCTGGGTCTATTCGGCAGCACATTGCTATCGGCTGTGTGTGCCACCCACTTCGATTGG CGCATGGCCATGGTTTCGATGAAAATGCGAGTGGGAGTGGTCAACTCCATATACAGGAAAGCACTAGAGGCGAGGGGCTT AAAGGAATCCAAGCCAGATTTGCTAAATCTTATGTCAACGGATACGGATAGGATTGTGAACTCTTGCATAAGTTTTCACT TCTTCTGGAGCATTCCCTTCAAGCTGTTTACCACATTGTACTTGCTGTATCTTCAACTAGGAGCAGCATTTCTGGCGGGA GTAATATTCGCTGCTCTTTTGATTCCAATTAATAGATGGTTGGCCAAGAGGATTGGCATATTTTCAAGTGGTTTAATGAC GGCCAAGGATGCAAGGCTATCTGCCACGACGGAAACAATGCAAGGAGCCAAGCAGATCAAGATCAATGCCTGGGAGGATA TATTCATAACAAAGATACGAGGACTCCGGCAGGAGGAGCTGCGATTTTTATCGAAGAGGAAATATCTGGATGCCATGTGC GTTTACTTTTGGGCCACCACTCCCGTGCTTATGTGCCTGCTCACATTTGGCGTATCCGTACTAATGGGAAATCCGTTGAT AGCTTCTACCACCTATACCAGCGTGGCCTTGCTCTATATGCTCATTGGACCATTGAATGCCTTTCCATGGGTGCTCAATG GTTTAATTGAAGCATGGGTTTCCATTAAGAGAGTCCAACAACTGATGGATGTGCCCAATTTGGATTATTCATCGTACTAT AATCCCATAATGAGAGGAAGCGGAGGATCCGGCGCAGGGGATGATGCGCCCTTGGATGCGCCAAAAGCCAGTGTGCTGCA GATGAAGTGCGCCAGCTTTTGTCATGATAGCGATGAACAAACATCTCCAACTCCATTCCGCATGAAGGATATAAATGTGG ACATAAAGGCAGGACAGCTTGTTTGCATCGAAGGACCTGTGGGTGGTGGTAAGAGTAGCTTTCTATCTGCCATTGTTGCC AATCTGCAGTGCACCGATGGCGAGGTGTGCGTCCAGGAATTGACAACTGGATTTGGTTACGTGCCCCAGTCACCATGGCT CCAAAGGGGCACCATTAGGGACAACATAGTTTGGGGCGCCCAGTTTGATGAACAGTGGTACAAAACAGTTTTGCATGCCT GCGCTTTGGAGGAGGATCTCCAAATCCTTGGAGGCGATCTGGTCGGTGTCGGTGAGAATGGAAGAACACTTTCGGGAGGG CAGAGAGCCAGAGTTGCACTGGCAAGAGCGGTTTATCAGGATAAAAAGGTTTACCTCTTGGACGACGTGCTCTCGTCTTT GGATGCCCATGTGGCCAGGCATATCATTAAGCACTGCATCCTGCGACTTCTTAAGCACAAAACTCGCATTGTGGTCACAC GAAATATTCAATTGTTCTTCCACGCCAATCAGATTCTTCAGGTGAAGGATGGTCAACTCCTTCCGAGTGAATACATGACC CAGAGCATTGAGCTAAGCCTAGATGAAGATGCGGACGATGAGCAGGAACCAACAGTCAGGCGACGTTCAGTGGAGTTATC CAACCAGTACGACAAAAAGAGTCTGGATAGTCTGCTTTTGGAAGAATTAAGGGAGTACGGACATCTTTCTGGAAACGTTT TTACATGCTACTGGAAGGCTGTGACTTCCCCCCTCGCTTTCACTGTTTTGCTATCTGTAGTCCTCATGCAATTGACACGA AATTTGAGTGATGCCTGGCTGGCTTATTGGGTCACTGAAACAACCTTGGATCCCCACAAGAATGATACATCCCTGGATCA CATTCTTATGCGACCGACAGTGGGCAATGAAACGGAGTCTGGGCACACAACAAAGTTCTATTTGAGCATTTTTACCGCCA TTGCTGTGAGCAATTCCTTGGTGACTTTGGCCAGGGCGTTTCTGTTTGCCTACGCTGGAATAAAAGCGGCCATCTTTATG CACGAGAAGCTACTGAAGAAAGTCATGTTTGCCAAATTCAACTTTTTTGACATCACCTCAGTGGGACGCATACTGAACCG ATTCTCTTCGGACACCAACACTGTGGACGATTCGCTGCCATTCATTTTGAATATACTATTGGCGCAACTTGCTGGCTTAG TGGGAGCTCTCTGTGTCAGTCTATATGCGATGCCTTGGCTGGGATTGGTGATTATACCCATGGTGCCCATCTACCTAAGC TTACAGCAGCGATATCGGCACGCCTCCAGGGACATCAAACGATTATCGAGCAATGCAATGTCACCGCTTTACACACATTT TACGGAGACTCTACAGGGCTTGACGACAATAAGGAGTATGCGAGCTAGTCCGAGATTTCAGCGGGACTTCCAAGTGAAAC TCGAGGAGAGCATAAAGGCTCAGTTGACACAGTCGGCTGCGCAACAATGGTTAGCTCTGCGACTTCAGTTGCTGGGAACA CTACTTGTCGGAGGAGCAGGTCTTCTGGCTGCCATTACCGCCTCTCACACAACGAATCCTGGCTTGGTGGGTCTATGCAT CTCCTACGCGCTCTCCATAACTGGTCAATTGGGAGATCTACTTCATGCTGTGGCTGAAACAGAGCAGGAACTGGTCGCAG TTGAGCGTATAGATCAGTATCTGCAGCTGGAGGAAGAGCAGAATGCATCCGGAAGTGCTGAGCCTCCTTTTGGATGGCCT ACCCAAGGAGTTTTAAGTTTTCGAGAAGTCCAACTGAGCTACAGAGAGCACTTGGCGCCGGCATTGAGAGGTATAACCTT CCAAACCGAAGCTTTCGAGCGTATTGGCATCGTTGGACGAACTGGAGCTGGTAAAACTTCTGTCTTAGCTGCCCTACTGC GGGTGGCACCATTGACGCACGGAGAGATTCGATTGGATCAGGTGAATCTAAAAACATTGCCTTTGAGTGTGCTCAGGGAA CGCATAGGAGTTATCACTCAGGAGCCCTTTCTCTTTGAAGGCACTGTGCGGGAAAATCTGGATCCGCGTCATGGTTTCTA CGACTCGGAGATCTGGCACGCCATTAAGAACTCCGCAGCGGCCACTCTGCTCGTTCAGCAGTTGGGCGGATTGGATGGAA AAGTTGAGACATGTGGCAACAACCTATCCGCCGGTCAAAGGCAACTTCTTTGTTTGGCCAGGGCCCTGCTGAAGAACGCC AAGGTGGTGGCCATCGACGAGGGCACTTCGAACTTAGACGACGAATCTGATCTGAGCATACAGCAGGCCTTAAGAAATGC ATTCAAGAGTTGCACGCTCCTGTTCATCGCACATCGCCTGCGAGGACTGCACGCCATGGATCGTATAATAGTGCTGGATG ATGGACGGATTTGTGAGGAAGGCAATCCCCAAAGCCTGGCCTCCGATAGCTCAACTATTTTCTACGGCATGCTATTGGCG CAGGACATTGATCCAGGGGAATTTATCAGATCGAATTAGCACTAAGTAACGATTATGAACTACGAATTGTATATAAGTCA ATTGTTACACAATTTTATTTATTACTTTGTTTAATTTTTATCATACTTGTGTTCCTAACTAACAATTCCATTTTGAGTCA CATTCAAAATTATGAAATTTGATGAGATCTAGCATCACTTCGCAATTTTTATTTAATTGTACAAATTCAAACTTGATTGA CATACTATTTTTGTAGTGAATTTAACAAAGCAGTTGAATCCGTTCAATGTGTACAAAAAACAAAACGTTATGTTATAAAT ACTACATTATAAAATATAAGACCTGTATTAATTACATGCTGAATAAATGTTGCGACCAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
