Comment: 0-22 hr AEL
TACAGGCTCGCTTCTTCGTCATGCTGCGAATTTGTTCGAAAAATAAGTGAAATACAGCGCATTAATAGTGAAAAGTATGT GCAGCCTGTGCAGGGCCCAACTGGCGATTAGCCGCGTGTGATTGTGGCCGCCGTATCAGCGTTTTGGCAGCGCCCAGCCT GGCGAAAAACCATCGAAAAAAAGAAGAGAAAAGAACGCCAGGCGCAGACAGACAAGACTTGCATACACTACTACTTGAAT CGTTTCATTTCGTTCCGTTCGTATACCGAGTTTGCTGCCCTGTCCCTGTCTTCTCACGCGAGTTCGTTTTTAGTATATAT AGCCAGTCTGTGGACGGTCGTCAATGCGTGAATATTCTTCTATGTCTAACATAACCTCAAACTCGCCGCTGCAGTCGTCG TCATCATCAGCGTCAGCGTTGTTGTTGTTGGTGGTTACCGGTGTTCCATCAGTAGCCGCCACACATCTGGGAAAATCCGA TCCGGAAAATCTGGGAGATCGGGAGCAGCGCAGCCAGAAGCCGATGAGCAGCCATGAATGAGAAAATAAAGTCGCCGCAA ACACAGCAGCAGCAGCAAGGTGGCGCTCCCGCCCCTGCTGCCACGCCCCCATCGGCTGGAGCGGCTCCTGGTGCCGCTAC ACCGCCAACCTCGGGCCCGCCCACACCCAACAACAACAGCAACAACGGTAGCGATCCCAGCATCCAGCAGCAACAACAAA ATGTTGCCCCACATCCATACGGCGCACCACCGCCCCCAGGATCTGGTCCTGGAGGACCACCAGGACCGGACCCAGCTGCA GTCATGCACTATCATCATCTGCACCAGCAGCAACAGCAGCATCCGCCACCACCCCATATGCAGCAACAGCAGCACCACGG CGGACCTGCACCACCACCGCCCGGAGGAGCACCTGAGCATGCGCCCGGTGTCAAGGAGGAGTACACTCACTTGCCGCCGC CTCATCCACATCCGGCGTATGGTCGCTACCACGCCGATCCCAACATGGATCCATATCGCTATGGCCAGCCGCTGCCAGGT GGAAAGCCTCCACAACAACAACAGCCACATCCTCAACAGCAACCCCCGCAGCAACCGGGGCCCGGTGGCTCACCTAATCG TCCGCCCCAGCAGCGCTACATACCCGGTCAGCCTCCGCAGGGACCCACGCCAACGTTAAACTCTCTGCTTCAGTCCTCGA ATCCGCCGCCTCCGCCGCAGCATCGCTACGCCAACACCTACGATCCCCAGCAGGCGGCCGCTTCGGCGGCAGCGGCAGCG GCGGCTCAGCAACAGCAGGCCGGTGGACCCCCTCCACCAGGACATGGACCGCCGCCACCGCAGCACCAGCCATCGCCGTA CGGAGGGCAACAGGGCGGCTGGGCACCGCCACCAAGGCCCTACAGTCCGCAACTGGGACCGTCACAGCAGTACAGGACAC CACCACCGACAAACACTTCCAGGGGTCAGTCACCCTATCCGCCAGCTCACGGTCAAAATTCAGGTTCCTATCCTAGTTCA CCGCAGCAGCAACAGCAACAACAGCAGCAGCAGCAACAGCAGGCGGGTCAGCAGCCCGGCGGTCCTGTGCCGGGCGGACC ACCTCCAGGTACCGGACAGCAGCCGCCGCAACAGAACACACCGCCAACATCTCAATATTCGCCGTACCCGCAACGCTATC CAACACCGCCGGGTCTGCCGGCTGGCGGATCTAACCATCGAACTGCCTACTCGACGCACCAGTATCCCGAACCGAATCGA CCTTGGCCAGGTGGGTCCTCGCCCAGTCCCGGTTCCGGACATCCCTTGCCGCCCGCCTCACCGCACCATGTGCCGCCACT GCAGCAGCAGCCGCCACCACCGCCACACGTCAGCGCCGGCGGACCTCCTCCCAGCAGCAGTCCGGGCCATGCGCCCAGTC CATCGCCACAACCATCGCAGGCGTCGCCCTCACCGCACCAGGAGCTAATTGGACAGAACAGCAACGACAGCTCCAGCGGC GGGGCGCACAGTGGCATGGGCTCCGGTCCGCCAGGCACACCCAATCCCCAACAGGTGATGAGACCCACACCCTCGCCAAC AGGCTCCTCCGGTTCGCGTTCCATGTCCCCGGCAGTCGCTCAAAACCATCCAATCTCGCGTCCGGCGAGCAACCAATCAA GCAGCGGTGGGCCCATGCAACAGCCGCCGGTTGGGGCAGGAGGTCCACCGCCGATGCCACCGCATCCGGGAATGCCAGGA GGTCCGCCCCAACAGCAGCAATCTCAGCAGCAACAAGCATCGAATTCGGCCTCGTCGGCGAGCAATTCGCCGCAGCAAAC GCCACCGCCGGCTCCGCCGCCCAACCAGGGTATGAACAACATGGCCACGCCTCCACCACCACCGCAAGGCGCGGCTGGAG GAGGTTATCCGATGCCACCGCACATGCACGGCGGTTACAAGATGGGCGGACCGGGCCAGAGTCCCGGTGCTCAGGGATAT CCACCGCAGCAGCCACAGCAATATCCACCAGGGAACTATCCGCCACGCCCGCAGTATCCGCCTGGCGCCTACGCCACCGG ACCACCTCCGCCACCCACAAGTCAAGCAGGAGCTGGTGGTGCCAACAGCATGCCCTCTGGTGCCCAAGCTGGTGGCTATC CGGGCCGTGGTATGCCCAACCACACTGGCCAATATCCACCGTACCAGTGGGTACCGCCATCGCCGCAACAGACCGTGCCC GGCGGAGCTCCCGGTGGTGCGATGGTAGGCAATCACGTCCAAGGCAAGGGCACACCGCCACCTCCAGTTGTGGGCGGACC ACCACCACCGCAGGGCAGTGGATCACCGCGTCCACTTAACTATCTGAAGCAGCATTTACAGCACAAGGGCGGCTATGGTG GAAGTCCGACTCCGCCGCAGGGGCCTCAGGGGTACGGCAACGGGCCGACCGGAATGCATCCTGGCATGCCAATGGGGCCA CCCCACCACATGGGACCCCCGCACGGACCCACCAACATGGGTCCACCCACTAGCACGCCGCCGCAATCCCAAATGCTCCA AGGCGGCCAGCCGCAGGGACAGGGTGCCAGTGGCGGACCGGAAAGCGGTGGACCGGAGCATATATCGCAGGACAATGGGA TAAGCTCGTCGGGACCAACGGGTGCTGCGGGAATGCATGCGGTCACATCGGTGGTCACCACCGGGCCGGATGGAACGTCC ATGGACGAAGTCAGTCAGCAGAGCACGCTTTCGAATGCATCAGCAGCATCCGGCGAAGATCCACAATGCACCACACCAAA GTCGCGGAAGAATGATCCCTATAGCCAAAGTCACTTAGCCCCGCCGAGCACGTCACCCCACCCAGTTGTGATGCATCCAG GTGGTGGCCCCGGTGAAGAGTACGATATGAGTTCGCCACCCAACTGGCCACGTCCGGCTGGTAGCCCTCAGGTGTTCAAC CACGTTCCGGTGCCGCAGGAGCCGTTCCGCAGCACTATTACCACGACCAAGAAGTCGGACTCGTTGTGCAAACTGTACGA AATGGACGACAACCCGGACCGGCGAGGCTGGCTGGACAAGCTGCGGGCTTTCATGGAAGAGCGGCGCACTCCGATCACAG CCTGCCCCACCATCTCAAAACAGCCACTCGATTTATATAGGTTATATATTTATGTAAAAGAACGTGGCGGATTCGTCGAG GTGACCAAGAGCAAGACCTGGAAGGATATTGCCGGACTCCTAGGCATCGGGGCGAGTAGTAGTGCGGCATACACGCTGCG AAAGCACTACACCAAGAACCTATTGACCTTCGAGTGCCACTTCGATCGCGGTGACATCGATCCTCTGCCGATCATTCAAC AGGTGGAGGCGGGCAGCAAGAAGAAGACGGCCAAGGCTGCTTCGGTTCCTTCGCCAGGTGGTGGACATTTGGATGCTGGA ACAACGAACTCAACAGGTTCGTCGAACTCACAGGACTCGTTCCCGGCTCCGCCAGGTTCCGCACCAAACGCTGCGATCGA TGGCTATCCCGGCTACCCGGGCGGCAGTCCATATCCGGTAGCAAGTGGACCACAGCCGGATTATGCGACAGCGGGTCAAA TGCAGCGTCCGCCCTCCCAAAACAACCCGCAGACTCCTCACCCCGGCTCACCCTACCCATCACAGCCTGGAGCTTACGGG CAGTACGGCTCGAGCGATCAGTACAACGCCACTGGGCCACCCGGCCAGCCATTCGGACAGGGTCCAGGACAATATCCGCC GCAGAACCGAAACATGTACCCTCCCTACGGGCCGGAGGGGGAAGCTCCTCCAACTGGTGCCAATCAGTACGGCCCCTACG GCAGCCGACCCTACAGCCAGCCTCCACCGGGAGGTCCTCAGCCGCCAACGCAGACGGTTGCGGGTGGACCGCCGGCCGGC GGAGCTCCAGGAGCACCCCCCAGCAGCGCCTATCCCACGGGCAGGCCCTCTCAACAGGACTACTATCAACCACCACCAGA TCAAAGTCCACAACCACGAAGGCATCCCGATTTTATCAAAGACTCACAGCCCTATCCAGGCTACAATGCTAGGCCTCAAA TTTATGGTGCTTGGCAAAGCGGTACCCAGCAGTACCGGCCACAATACCCATCATCGCCTGCTCCGCAAAACTGGGGAGGT GCTCCGCCACGTGGTGCTGCACCTCCGCCTGGCGCACCACATGGACCGCCCATCCAGCAGCCGGCAGGAGTGGCTCAATG GGATCAGCACCGCTATCCGCCTCAACAAGGTCCTCCGCCGCCACCACAGCAGCAACAGCAGCCTCAACAGCAACAGCAGC AACCGCCGTATCAGCAGGTGGCTGGACCTCCTGGGCAGCAGCCGCCTCAGGCGCCGCCACAGTGGGCACAGATGAACCCC GGGCAAACTGCGCAATCAGGAATAGCACCTCCGGGCTCACCACTTCGCCCGCCCTCGGGGCCAGGCCAGCAGAATAGGAT GCCCGGCATGCCAGCACAGCAGCAGCAGTCGCAACAACAAGGTGGAGTTCCGCAGCCACCGCCGCAACAGGCATCCCACG GCGGCGTTCCATCGCCAGGATTGCCACAAGTGGGACCCGGTGGAATGGTTAAGCCACCTTACGCTATGCCACCGCCACCA TCACAGGGTGTTGGTCAACAAGTGGGCCAGGGGCCACCCGGCGGCATGATGTCCCAAAAGCCACCGCCGATGCCGGGACA GGCAATGCAGCAACAGCCTTTGCAGCAGCAGCCTCCCTCGCACCAACACCCACACCCCCATCAGCATCCACAGCACCAGC ATCCGCACCAGATGCCGCCCAACCAGACTGCCCCCGGTGGATACGGTCCTCCAGGAATGCCCGGAGGTGGAGCGCAGCTG GTAAAGAAGGAGCTGATCTTTCCACATGACAGCGTGGAATCCACCACGCCTGTACTTTACCGAAGAAAGCGGCTTATGAA GGCGGACGTGTGCCCGGTGGATCCATGGCGTATATTCATGGCCATGCGATCTGGTCTGCTGACCGAGTGCACGTGGGCAC TAGATGTGCTTAACGTGCTATTATTCGATGACTCAACAGTCCAGTTCTTTGGCATTTCAAACCTGCCCGGCCTGTTGACT CTGCTGCTTGAACACTTTCAGAAGAATCTCGCCGAAATGTTCGACGAACGGGAAAACGAGGAGCAGTCCGCTCTGCTGGC GGAAGATGCGGATGACGACGCGGACAGCGGCACTGTGATGTGCGAAAAGCTGCGTACCAGCGGACGGCAACCTCGATGTG TGCGCAGCATCAGCAGCTACAACCGCAGGCGGCATTATGAGAATATGGATCGCAGTGGCAAGGATGGAGCTGGCAACGGC AGCGACTCAGAAGACGCCGACGAGGGAATTGATTTGGGTCAAGTGCGAGTACAGCCTAATCCTGAGGAGCGCTCACTGCT GCTCTCCTTTACGCCCAACTACACGATGGTCACGCGAAAGGGTGTGCCAGTGCGCATACAGCCCGCCGAAAACGATATAT TTGTGGATGAGCGCCAGAAGGCGTGGGACATAGACACCAACCGCCTGTATGAGCAACTAGAACCCGTAGGTAGCGATGCG TGGACGTACGGGTTCACAGAGCCAGATCCCTTAGACGGCATTATTGACGTCTTCAAGTCGGAGATTGTTAACATTCCATT TGCACGCTACATCCGCTCTGACAAGAAGGGGAGAAAGCGAACGGAGTTGGCTTCCTCATCCAGGAAGCCAGAAATAAAGA CGGAGGAGAATAGTACCGAGGAACAGACGTTTAACAAGAAACGGCGATTGGTTAGCGGAGGCAGCAGCAGCAGCGGAGCC CACGCCGAAGGCAAGAAGTCCAAGCTGACGAGTGAGGAATTTGCCCAACCAAACGCCGAAGTCAAAAAGGAGCCAGGTAC GGCGGACAGCGATTGTCGCCCCGTTGATATGGATATCGAAGCACCCCAACAACGTCTGACCAATGGTGTGGCACCCTGCT CCTCGACTCCCGCCATCTTCGATCCCCGGACGACTGCCAAAGATGAAGCGCGGGTGCTTCAGCGAAGGCGTGACTCCAGC TTTGAGGATGAGTGCTACACACGAGATGAGGCGTCGCTCCACCTGGTCAGCGAGAGCCAAGACTCGTTAGCGCGGCGCTG CATCGCTCTCTCCAATATCTTCCGCAACCTGACATTTGTGCCCGGCAACGAGACGGTGCTGGCCAAGTCTACCAGATTTC TGGCGGTACTTGGTCGACTGCTGCTCCTGAACCACGAGCACTTGCGGCGCACACCGAAGACGCGAAACTACGATCGCGAG GAGGATACGGACTTCAGTGACTCGTGCAGTTCGCTGCAGGGCGAACGTGAATGGTGGTGGGACTATCTGATTACCATTCG GGAGAATATGCTTGTTGCCATGGCCAACATTGCTGGACACTTGGAGCTGTCGCGCTACGATGAGCTAATTGCCCGCCCCT TGATCGACGGCCTGCTGCACTGGGCCGTATGCCCGAGTGCTCATGGACAGGATCCGTTCCCGTCATGCGGACCCAATAGT GTTCTCTCGCCACAACGACTGGCA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
