Comment: 0-22 hr AEL
CGACAACCCTGGGAGCAGGAAGGTTTTTCGGCCGAAAAATAGAGAACAATAAAACGTTTGTGGCCCTCAGCAATATAGGG ATCGCCGAGTGCCGTGTTAACCGGTGACCTCTCGCCCGTGCCCCGAGACCTGTGACCCCGCGCAACTGCCCGCCTCAATG TTAATTTGTGGCAGCAAAAGCAGCAGAATTTGGAGCAGCAGCGTGTAAAAAGTTGCGGAATTATCGTGGCACCGCGGAGA AAGAGAGAGCAGGAGAGAGATTGCCGCACGCATAAAATATGATCAAATTGCAAAATTGATTTCAATTGGCCACTCAACAA AAGCAAAAACCAATTTCAATGTTTGGCGCCTGCTGCGACTCTTGAGACTCGTCTAAATCCGCAAGTACATTCAACCGGAA TTTGAGCCAAGTTTCTAACCTTTGGCCTCAAAGCCATGGCATCCACGTCGTCGTCGGCTTCCACGACGGCGGGATCCTCC GGCGCGGCAGGCGGAGGAGCAGCCACGCCCATCTCAACGCCCTCGACGCCCCAGCCGTCTGGCGTGAGCAGCGGACCCGG CAATAATGGCAACAACAACAACAACCATCACAACAACACCCTGGTCTCACCATCCGGGAACAACAACTTGATGACGCCCG TCCAGGCATTCACTCCAACCGGACAGCCAGTGTACAATCCACCCGTCTATATGTCCGCCCACGGAGGACACCCGCACGCC GGAGCCCACTACCCGGCCATGATCCCCGCTCCGATGCCCAGCAATCACGTCTATGTGAACAACGTCACTGCCAATGTGAA CCTGCATGGCTGGCCGCACGGTGTTCCGGCTTATATGCCACCCGGAGGTCAGCATCACTACATTGGACACGGCGACATGC CCCAGGAGCAGGGCAACACGGTGATGCCGCACCTGCAGCCTGTGCCCATTAATTTGGCGCCCCAGGGCGGATCTCCGAAT CCCCATGGTCCTAGAATGGGTGGCGGACGGATGCGCGAACGAGGTCGTCCCCGTGGCGGTGGACCGCGACGCAATGACTA CCATGGGCCACGTCACCTCCAGCAGCAGCAACTGATCACCCTTCCGCCGCAGCAGCAACTGCCACCGCCGGACGGAGCCA ATCCCGGGCTGCAGCTTCAGCCGGATCAGATTGGTCAGCAGTTGGCGCCGCCGGAGGCGCACATGCAGCACTTACAGCAG TACTACGCGGCGCCACCAACCTACGCCTACGGGCCATATCCCTCTCCCTACTTCACACCCCAACATGCCAATGTGCAGCC TCCCCCCAATGCCACTGCTGCCCAACAGGCAACTGGAACGCCGCTCTTCATCTCCGGACCGCTGATGTATAATCCTGCGT GGTTCAACCACGGTGGGTACATCTACCCCATGATGCATCCGGCTGAGTACCAGTATGTACCCGAAGACGCTGGCCAGCCG GGAGTGGATGAACGTGGAGCTCAGCCGGACGGCGGAATGACCCAGATCTGGCACCAGGGCCCCATGTACGCAGAGGACTT CGAAGCGCTACAGCAGCAGCAACAACAGCAACAGGTGGCTGTGCATCCCAATGGAGGTGCTGTCAGTGTAGCCGATGAGC TCAACCACAATAGCTCATCCCTGCCCTCCTCGGAGACGTCGAGCATGATCAGTCCAAACTATCCCATCTACGATCCACAG ATGCACGAAATGCAGCACCAAATGGGCGTGATGCAGATATTCGACGATGGTCAGATGGCCGCCCTGCAGCCAATCCATCC AGGAGCGGGTCCGCTACCGCAGTACGAGGACGAGCTAGCCGAGTGCGGAGCACCGCCACCAGGCCCTATTCCGGTGGCCT GGACTGCTGCCATGCCCCTCCCTCCGGATGCAGTCCAGCCAGCTCTGCTGCCGCCACCGCCGCATCACATTTTGCAGCAA ACGTCGCCCCTGTTAATCGAACAGCCAGCCCAGTTGGTTCAGGCGCAACCTCCGCAGCCACCAACACCCACTCCCCAACA GCAGCAGCAACAACAACATCAGGAGCAAACGGCTGACAAGAGTTTGACACAGAACAACAACGAAGTGGTGGCCTTGGATG AAAAGTTGGCCAACGAGAAGCAGCAGCAAATCGATTTCCACCAGCAGCAACAGGAGCAGCAGCAACAGCAGCAGCAACAG CAGCAGCAGCAGCAGCTTCATCCACTAGCACAGCAGCAGCAGCAAGAAGAGCAGCAGCAACCGCAGCAACTACAGCCGCT GCAAACCGAAAAACCGCCAACGAAAGTGCCAAACCTTGTTGTAGCTACAGTGGCACCTCAGCTGCAACAGCCACCGCTGC AGCAGCATCAGCAACAGCAAACATCACCTGTGGTGGTGTCGCCAAAACAGCAGCAGCAGCAGTCACAAGCTGCGCACCAG AATAGCTACCATGATCAGCATCCTTTGCCGCAGCAGCAGCAACCGCAGCAGCAGCAACCGCAGCCACAGCGCAAACCCTC GCAGCAATACAATCAAGCGCAGCAGCAACAGGTGCGTCGTAAGTACTCCTCAGAGTACAATCACCATCATCACCAGCACG GCAGCAGCGACACGGGCATCCAAACTACCAAGACCATGTCCTGGACCAATTCAGCTCAGCACAAAAAGTCGACGCAGTCG GTGTCGGTCACCGCTTCTCCCAATAGTGTAAACAATGGACCTGGAGTGGGTGCTGCGGGCAAAGCCAACTATAGTCACAC CACTAAGACATTTACCAATCAGCAGCATTACCAGCAGCAACACTATCACACTCAGAACAGCTATAACAACAACAGCAGCG GCGGAGGAAGCAGTAGCAACAGCAGTTCGAGCAGCAGCAACAATCAGCCCCAGGTGCGCAACTATGGCACCATGAAGATG CCCTCTTCGCCGGTGGCTTGGACAGCGCCAACACCGGAGCGCAAAAACAGCCAACAGGCAGCAGCGGCACCGGCAGCAGC AACAGCGTCTGCAGCTCCCGTGGCAATCACAACAGTAACTACCTCAACGGCCAGCATTACGGCTACCCCAAATCAATTTC AACCGGAGACCGGCAGCAGTAGTGCATCACCAGCCGTAGCTGCCGCGCAGCAACAGCAGCCTGCAGCACAGCAACATGCG CAGGCCGCACAAACAGGAGCAGCAGCAGCTCCAAGCAAGTCCCACAGCAACTATGCTTCTTCTAAAAAGCATCAGAGCTA TGAGCCAGTAGTGCTGAGCTCCGTGGTGGCCACCAGTTCATCAGCCAATCCGCAGCCGCAGCTAAATCTTGCCCCACCTG CGCCACCAGCCTCGCAGAACAGCAGCGACAGTTCGCAGCTTTCGTGGGCCAGTTTGTTTGCCAGCAACAAGCCCGTGGCC AAGGTGGCGCCCTACGAAGCCAGCAAAATTGCACAGCAGCAGCCATCCCATCCAGTGCTTCAATTGGCTCCTCCGCAGCC AGCTCAAGTGGCGGCTGCCGCCCCAGTTGCTCAGCTGTCGTCGCCTCCTCAGAATTTGCCCCTGCCTACGGCTCACCAAC AGTCACAGTTACCAGCACCTGTGCCGGCGCCCACGGCTCCGCTGGTCACTCCAGGAGCGCTCTCCTATTCGGCTGCATCC GCTCAGGCAGTTCCTGCTCCCAGCCCCGCGTCGGCATCCGTCAAGCCCCTCAAGCCGGAACCACCGCGTCCTGTGCAGCA GCAACTGGATGAGTGGACCAGCAAGTACGCTGAGTACCTGACGCGCCACAAGACCAATTTGGCGTCGATCAGCTTGCGTC CCCGAGGACTGACCAACAGGTCGAACTATTGCTACATCAACTCGATCCTGCAGGCACTGCTCGGCTGCTCGCCGTTCTAC AACCTGCTGCGCTCCATTCCCAAGCAGGCGGCTGTCCTGAGCGAGGTCAAAACGCCCACTGTGAACGCCATGATGTCTTT TATGACCAACTTTTCATCGTTGCCCAGCGGTCTTCGCCTGCGTCTGAACAACCTTAACAAGGGATCGCAAAGCAAGGGCA AGGACGATTTTGTGGGCTCAGATTTGCAATGCGACATGGCCTTTGAACCCACAGAGATCTACAAGCTGTGGAACGACAGT CGCGAGGAGCATGTCGAAGGTCGTCAAGAGGACGCCGAAGAATTCTTGGGCTACGTTCTAAACAAGCTGAACGACGAGAT GCTGGAGGTGATTAAGCTAATTGATAAGCCCACACCCCAGCAAAATGGTCAGGAACCCGCGGAACCAGAGGATGGAGGCG ATGTCTGGCAGATGATTTGCAACAATCGTAATAAGGGCAGCGTAACCCGCCAGACCGACTTCGGACGCACTCCCGTAAGC GATATCTTCCGAGGAGAACTGCGCTCCCGGCTGCAGCGTGAAGGAGAACACTCCACGGATGTGATTCAGCCTTTCTTTAC GCTTCAACTGAATATCGAAAAAGCTGCATCTGTGAAGGAGGCACTGGAGATCCTTGTGGGTCGCGATCAGCTGGAGGGAG TCACAGGCAGCAAAACCAAACAGGAGGTGGTGGCCTGGCAGCAGATGACGCTGGAGAAACTGCCCGTCGTTCTGATATTG CATTTAAAGTACTTCGACTACCGCTCTGATGGGTGCACTAAGATTTTGAAGAAGGTGGATTTCCCCGTGGAACTAAAGAT CGATGCCAAAATCCTCGGCTCAAAGAAGACCTCGCAGAAGCAGCGCGCCTATCGTCTGTTTGCGGTTGTCTATCACGATG GCAAGGAGGCGTCGAAGGGGCACTACATCACGGACGTTTTCCACACGGGCTACAGCAGCTGGCTGCGCTACGACGACAGC TCGGTGAAGCCGGTCAGCGAGAAGCATGTCCTTCAGCCGCACACGCCTCGTGTGCCTTACCTGCTGTACTACCGCCGTTC AGACACCCTGCCACCGCAACAGCAGCAAACCCAGCAGCAGAATGGCGGAGGATCAGGTGGAGTCGTGGGCAGCAGCTCAT CATCATCGAATGCCGGGGATAACAAGTGAAAGTGAACCACAACGCGGCGTTGTAAAAATACATCAGAACTTTAAGTGCTA GCGATATACTTTATATTTTTGTGTCAGTGCGTGTGAGCGAGCGAGAATGCGAGTTTGGTTTTATATGCATATACATATAC AAATTAACAATTAGATATATTCGAGCGGTATATAGTTAAGCCCAAAGTCCAAAGCGCATGACCGTTTGTTAGAGTACGAT TGTGTGAGAAAGAAACTTAGCTCTAAGCTAGACTAGAATTAAGGTTGAGTAGAAAAATAGGCAGCAAAAGAAACTCGTTG GAACGAAAACTTTGTACAAAGAGAAACGAAAGAACTCTGTAAACTAAACAGCGAGTAAATAATTAACATATAACTTAGTG CTAAATTTACAAAATCCTTCAGCGTAGCGAAACCCCAAAATATCTGTGGCACAAAAGCCCTCCCAGATGTTAGAAGTAGT GGATGTTAGAAATGCCCTTAAATACCTTTTGGAATTTGGCGATATGTTATGACAAACGCTTACAGCTGTATATATACACA AATAATCATATATTGTAGTTGATCAGTTTCGCGGCATGCACCATGCATTTAAACAAACAAACCCAGCAACAATATATATC GAATGCTTTGAATATGCTTTAAAGAAATATACATTTAGAACCAAGGTGTTTTCCACACAAAGAGTAAATACAGGCAGTGG TGGTCAGAGCAATAGCCGCAAGCATTTCAGCATCTGTAAGCCAAATTGGGTTACTTTTGTTATGGTCTCGAAGACCAAAT CAAATAGATATTTCCTTTACTAACAATTTTTGCTCATGTAGATGGAAGTTTAAACATGAAAGTAACGAGCTGAATAAATT ATCTATACGGCTTTTCTAGACCACTACTGCATGTTGCATTGCATATAAGCAGCAAAGTAAGTTAACAACGCGTTCATTTT GTAATTTATTTTATATGTTGTTTAGTTTTATACGTAATCAGTTAGGCGAGCAGATTCATATATACACAAACGGAACTTGT TATTCATAGTTAGAGAAACTTTTTAGGGATTGTCTCCCCGGCTTGGATGCGATGTTTGCCTTCATGATATTCGAGTCCTT AACTTTCTTTTCTCAGACCTAGAGCATTTAGTTTGTACATCATGTTAGAGAAATCTGTTGGGATTGTAACAAAAGATTTG AATGGGAATAATTATTAAATGTAATCGGGTACGAACTAAAGCTAATCAACCATACTAGTTAATAGATGTCTTACAAACGT ATACACCCATATATATTTTCTTAAGCTATGTAATCTAAGCGCATATATATTATTTATAGATATCGTGCCAATGTTAAAAC ATCTAAAAATTTTCAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
