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FB2026_01
,
released March 12, 2026
Comment: 0-22 hr AEL
ACACGCTTTCTATTTGATTTTTTTTTCAACATAATTTTTGTTAAACAATTTGCCGTGAATTTTGTTGTGTGTGTGGATGT GGACGTGCTGACAGCCAAAAAAATTAAAATAAAAAAAAAAAGAAAAGTGTTCGCAAGTCGAGAGCAGAATCGAACGGCAA AAAATGCTGGCGAACAACAAATCATCAAGGTAAAACTGCGCGCCTTGGTCATTAAGTCTTTCATCGAGGATAAAAGACCG ATGTCTTTTAACGTTATTGCTGTAAGCAAAAGCAGAAATCACAATCTACTCATAAATCCTCGATTTGGTGCAAATTAAAG GAAATTCATCGGTTTTTGGCGGCCAGTTGCAAACACAAAATACTAAATACGCTAGATGGAGCACGCATACACGCAAGCTC GTTGGCGAACGTAAATTACATACATCATATAGATAGTCGTCCCGCTTGCACTGCCCGTCACAGCGAGGGCTGCGAGAGCG AGAGCGGGAGAGAGAAAGGCCTGAGTCGCTTTTTCTTCTTGTACTTTATATATTTTTTATTGTTTTTTTGTGTTGTGTTG CGTTGTACGTGTGTGTGAGAGTGCCAAATGTCAACGGAAATTACAACACTGCGAGACGGAGAAGTCTAAAAGGCAGAAGA AGAAGAAGCAGCAGCAGGCAGCATAAACAAAACTCGGGGGAAAACCACAAAAAATAACAGAGGGAGAAGCAGGAAGATGT TGCCCGCCAATAACAGGAGTAGCACCAGCACCCATACCAACACAAATGCCAACACAATCAACGCCACTACCAATACCACC AACAGATGCCTCATCAATACGGCCATCGAAAAAACGGTAGTCCGTTTGCGAGAGACGGCAGCGAATAGCGGTACTTTCCA GGACTCAGATGAGTCGTCCGTGGAATTTGATATTATTAACTATTATGGGGACGACGGCGGCACAACTCCAGCACCAGCTC CAGCCACAGCCTCCGTTACTCGCCACGGCGGCAGCAGCAGCGGCAATAACAACAATAACAGTGCATGCCATCCAGCACTG GATGCCAGCAGTGATGTTGTTGTTGTTGAACCGGCAGCGGTAGGAGTCGCACAGGAGGAAGAGGAAGAGCCGGAGCAAAG GCCAGAGAGGATCAGCATACCCATTCCCGACCTGGCGTTCACCGAGATGGAAGCATATGCCGAGGATATAGTCGTCGATA TGGAGGGGGGATCACCAGCCAAGCCTTTAAATCCAAAGAAACAACGTTTAAACTCAGCAACAACCACAACAATAAATCGC TCGAGGGGCGGCGGAGCGGCACAGAGTCGATTACGCCGGTCGGCGGCCATCGTTCCACCGCGATCGATTCCAGAGAGCTG TGCCAGCAGCAGCAATTCCAATTCGAGCAGCAGTTCCAACAGTAATTCCAGTTCCAGCTCCGCTACAGGAAGTAGCGCAT CCACCGGCAATCCGTCGCCGTGCTCCTCCCTGGGCGTCAATATGCGCGTAACTGGACAATGCTGCCAGGGAGGCCGGAAA TACATGGAGGATCAGTTCTCGGTGGCCTACCAGGAATCACCGATCACCCACGAACTGGAATACGCATTTTTTGGCATCTA CGACGGACACGGCGGTCCCGAGGCCGCGCTCTTCGCCAAGGAGCACCTTATGCTCGAGATCGTCAAGCAGAAGCAGTTCT GGTCTGATCAGGATGAGGATGTCCTGCGGGCAATACGCGAGGGATACATCGCCACACATTTCGCCATGTGGCGGGAACAA GAGAAATGGCCACGCACTGCCAATGGGCATCTGAGCACCGCCGGCACCACCGCCACAGTGGCCTTTATGCGTCGCGAGAA GATCTACATTGGTCATGTGGGTGATTCTGGGATCGTTTTGGGTTACCAGAACAAGGGCGAACGCAACTGGCGTGCTCGTC CACTGACCACGGACCACAAGCCGGAGTCACTGGCAGAGAAGACGAGAATCCAGCGTTCCGGCGGCAATGTTGCCATCAAA TCGGGAGTTCCGCGAGTGGTATGGAACCGACCCAGGGACCCAATGCATCGCGGTCCCATTCGCCGCAGAACTCTGGTAGA TGAAATACCCTTTTTGGCGGTGGCTCGTTCCCTGGGCGATCTCTGGAGCTACAATTCCCGCTTCAAGGAATTCGTTGTGA GTCCCGATCCGGATGTCAAAGTGGTTAAAATAAATCCCAGTACCTTTAGATGCTTAATTTTCGGCACCGATGGCCTGTGG AATGTGGTGACCGCCCAGGAGGCGGTGGACAGTGTGCGCAAGGAGCATCTAATCGGCGAGATACTCAACGAGCAGGACGT TATGAATCCCAGCAAGGCGCTGGTGGATCAGGCCCTCAAAACCTGGGCCGCCAAGAAGATGCGTGCGGACAACACGTCCG TTGTGACTGTGATACTAACACCAGCGGCCCGCAATAATTCGCCCACAACGCCAACACGTTCCCCATCCGCGATGGCACGC GACAATGATCTGGAGGTGGAGCTACTGCTGGAGGAGGACGACGAGGAGCTGCCGACACTGGATGTGGAGAACAACTACCC TGACTTTCTCATCGAGGAGCATGAGTATGTGCTGGACCAGCCGTACAGTGCATTGGCCAAGCGACATTCGCCTCCGGAAG CCTTCCGCAACTTCGACTACTTCGATGTGGACGAGGACGAGTTGGATGAAGATGAGGAAACAGTGGAAGAAGACGAGGAG GAGGAGGAGGAAGAGGAGGAAACCAAATCGGTGGGAATTCTACAGCAAAGTTTGTTCAACCCCAGAAAAACGTGGCGCAA GTCAACCATCAACAATTCCTGGAGTGGCGTCACCGAACCGGAACCGGAACCCGATCCCGAACCAGATCGAATAGATGTCT TAACACTGGACATGTACTCCCACACCAGCATTGACAAGGGCACCAATTATGGCGGCAGCATAGCCCAGTCCTCAATAGAT CCTGCGGAGACGGCTGAAAATCGTGAGCTGAGTGAGTTGGAGCAGCATCTGGAGAGTAGCTACAGTTTCGCCGAGTCGTA CAACTCCCTGTTAAACGAGCAGGAGGAGCAGGAGGCACGCTCACGTTCAGCAGCAGCAGCAGCCGCCGCCGCAGAAGCAG CAGCAGTAGAAGCACAACAAACCACTGCCCATTCCGCATCCGTTGTGCTGGACCGCAGCATGTTGGAGATCATCCAGGAG CAGCAGCACTATCAGCAGCAAGAGGGCTATTCGCTAACGCAACTAGAGACCAGACGTGAAAGGGAGCGGCTGACCGAATC GTGGCCACAGCAGCCGGCTGAGCTGCTCGAGCTGGATGCTCTACTGCAGCAGGAGCGTGCCGAGGAGGAGCAGGTAGCCC TGGAGCAGCAGCAGCAGCGCGAACAGCAAATGGAGCAAATGGAGGTGGAGGCCATTAGTAGTTCGGGACAGCACGAATTT GCTTACCCAGTGACCACCGCCACAGCCAGCGAGTGGTGTGCTACATTACAAGAAGACGAGGAGGAGTTGGACTCCACAGT AATAGACATAGTAATTCAACCCGAACAAGAGTTGCAGGACAATGAAGTGAGCTCCACGTTGCCCGCCACACCCACTCATG TGGAGCCTGAGCAGATTGTGGACAAGATGGAGCCCCTGAAGGTTCAGGAGATGCTAACCGCGGTCGAAAAACCTCCATCC AAGCAGGAAAAGAAGCTGCCGAAGAAGCAAGAGACCAAACAGGTTGCTGTGCTAGATACAGTGGCCGAGATGCCCAAAGA GGATGCCCATGCCGTGCACTATATATTCCAGCGCATTCAAAAGGTTCAGGACTCTGAGGCAACACCAGTGGCCGTGACGA ATTCCACAATGGCTGACGCCCTGCCCACCGAATCTAGTGGACTGGGAGGATCTATGACCGCGCCCCGAATCCGACGCTAT CGCAACGTGCCCAACGAGAACCATCAGCACATGCAGACGCGTCGTCGTCAGATCTTCAAGCATGTCAAGCCAAAGTCCTT CATACAGTCCAGTGCTGCGGCGATTGTGGCCTATGGAGACAGCACCGAAACGGTCGGAGGAACAGCCGGAGCATCTGGCA CACCTGCAGCTGGGCGTGTAGGCGGGGGCGGTGGCGGCGGCGGCGGCAGAGGATCGGCCAGTGGTGGGAGCAGTCCAGCG GTGGCAGCCAATAGTCGGCGGAGCGTCAATGTGGTGGCCAATGCGAGTGGAAACAGCGCTAGCAAAGTTGTGCCCAGCAG CAGTTCCATGATGATGACCCGCCGCAGTCACACCTTGACGGCCAGCGGTGGTGTGAACAAAAGGCAGCTGCGCAGCAGTC TCTGCACCTTGGGCCTGGGTGTGGGTGTCGGTGTCGGTCTGGGCATGGACCTGGACATGACCAAGCGCACGCTAAGGACA AGGAATGTACCCGCTTTGTCGGGCGGTTCAGCCACGCCATCTAGCAATTCGTCGCCAGCCAGCGGAGGCAGCAGTCCAGC CGGTTTCACAAGCCCAGCCAGTCCGGTCATCACGTCCAGGGGAAGCGGATCGCGTACTACCGCCTCGCCAGCCAGGCGCC TAAAACGCAGTCATGAGGATCGGGAGCAAAGAATGAGCTTGCGACGGAGCACTCTGAGTGGCAGTGCCAGCGGCAGTGGG CTGGTGGGCACTGGTGGGTCGCCCTCGAATGTGAAATCAAATCGCCTGCAGGCCTGCAATGGAGCCATCTCTGCGCGTCC GCCGCCCTCGCCGAAGAAACTGAATGCAGCCGTGCCCACATTGGCAATTGGAACGCGTGCATATACGGCGGCGTTGGCGG CGGCGGCGGATCACCTGAACAAGCGGTGGTCGTTGCGCAGCAGCAGTGGCAACTCTGGCAATCTGATAACCGCCATCAGT TGCTACAGTGACAGGAGCAGGGCGGCGACTGCGGCGGGATCACCGGGATCTGGAGGCGGGGCAGCGGGACCACCAGGAGC ATCTTTGGCCGCATCCACAGTCGGCACGCGAAGGCGCTAGGCTAGATTGTAACGAAACATGCGAGCAACTTGCAAGTACA AATCCTAAGCAACGGAAAATTTTAGATCCTAGTATACTACTTTACTGAAAACGCAAAATTGCATAATTTAACCAATTTTT TTATGTGCACAACACACACACACACACACACATCTTTTAAATGTATATATAAAAAACTGTATAAGCCGCCGCATAAAAAA AAATATATATATTGATATATATACATTTAAAAATAAAAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.