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FB2026_01
,
released March 12, 2026
Comment: 0-22 hr AEL
TGAAGATTAGGAAAATAATAAGGAAGGCAACGAAAAGTAAAATAAAATTTGATGTACAAAATACGCAAATTGGTGACAAA CAATGTACAAGTGGCATACATGAAATAAAGTTTTATGCAAAAAAAACAAAATCGACTGCAGTGCGTGCACTTGTGTGTAT GTGCCGCTGCATATGTGTATGTGTGCAGTGTGGAAATAAAGCAAGAGAGAAAAAAAAACTGTTTGCAGCCGGGGTACAAA TTCCAATTGGAGTCTCAAGGAGCATAAATTCCCATTCGACGCATGCGTGGCTGCTAAAAAAAGCAACCGAAAGTGAATTG AATGGCATCGAATAGATGGAATGGATATGTGAATGAATTTCGACTTTCGCACAATTTGCGCGCTCTTCACACTCGATTAT TGGAATCGAATTAGATACGCAAACTAACCGACATTTTATCAAAATAACGAAACAAGTATTTTGCATAAAAACGAACGGAA ATCGAAAGGAACGCCCTGAAGGTCATTGCCAGGGAGCGGAACGCCGGCAACGAAACGGGAAACAATGGAAATAAGAGGAA GCCAGGCAGACATGCAAATATTGGGCGACTTTTGAGGGGTTCGATTAACATAACTCGCTCTCTAAATAAAACATTGTACA TCCCCCCATGCACACATGGTTAATACAGCCAAATAAAGTTGGAAAACAATGGAAATGCAACAGGAAAACGAGACGGGAAG AGAAGGGGTAATCCCGAACTCGGAGAAAGAAGTAGCGTTGATAAGTAACCAAGAGACTGAAAAAGCGGCCATGATTAGTG GTGGCAAAGAAGGTTCGGTAAGGGATTTGGAGTCCCCAAACGAAACCGCCACCACCCAAAAAATAGAACACACAACGAAA CCACTTAAATTGGATGGCATTTTTGCCATGCCAGCCACTCCGCCGAATAGGGCCGGACTAACAACAAAATCGGCCAGCAG TAGCCGTAGCAGTTCCCTCAAAAAGCAGAGACGAGGATCCAGAGGAAGTGCCAATTTGAATGTAGCGGGAACGGGTGATG GAGCACCCAAAAGAAGCGCCCCCGGCGGTGGTCAGCTAAACTATGGCACCAATAGTGTTGGAAACGACCTTATGAAGCAA TCGCAGTCCATGACGTCACTGAAGGCCTCTAATGAGGAACAGCAGGAGAGTCGTGACGTCATCCAGGCCAAACTTCACTT GGAACGTCCCTACAACAGCCTCAAGAAAAATTCGCATAGCAATAAAATGCACCGTTATTCGTGGGGAAGCGGAAACAGTC AATGCAGTTCCACCAGTCTGCACAGCAGCGCCACCTTGGGTCTGGGTTCGTCTGGCAAAGACGACCTCTGGGCGGCCATT CAAACGAACTACAACTACATTATGGACACGAACCTTTTGGACACTTGCAAGGAGGCGCGCTGTGAGATAGAGGGCGCTGC CACGGTGTTGGAGAAATCCAGTGAATGTTCCTTTAAGATGCTCGATGAGACCCAACGACCGGAGGGTCTTTGTGAGGATC CCAAGGAGTTGCGGCGTTGGCTGCGCGAAATGGAGAATAAATTGGAGAGTTCGCCGACAATCACAGAGCTGACTTTGTTT GGCAACGCGGAATTGCAGCGCCATCTAGCAGTACACTCGGTGTTGTACCATGAAATTGCATCACACGCTCGAGTGGTGAG CTCTTGCATTCGAGCCGCCGAAAAGGAACAGCAACTCCAACAGCAGCAGCAACTGTCGAGTCAGCAACCCGCCTCATTGA CCAGCAACTGCTCCAGTGAGTCAACCAGCGAATCGGCCACAAAGTCGAGCAGCCTGAGCAGTGGTTTCGCTTCCGATCCG GTGACCACACCCATTGGAACCGCAGCAGCGGCCCCGCCGTCAAGTAGCACCCACCCATCCAAGAAGGGGGAGGGCAACTT GGAGCGACTTAGGGATCGCTACCATCTGTTGTATCTGAAGGCGTTCGAACTGCAATTGTGCCTGGATAATCTGCTCAGGA AACGCAGTTCCGCAGCGAATGGTTTGGATGACGACGAGGACGAAGAGGAGGACACCGAAGACGATTCCTTTGGCTACGAG GGCGAAGCCACCGAGGACGATCTGAACGAGGTCAACTCGGATCTCGATCTAGAGAACAGCGAGTCCAAGTCGGCCACCCC CGCGGAACTTATTCTCCAGCGCTGTCAGATTGCAGCTACCCAGATTGTGTGCGGACTGGATGAGACCCAATCCCAGTCGC GGTCACAGCAAGTCCCATCCCAAGCGAAGTCGCCGTCAGCGGATCAGCCACGCGATTGTCTTGCACCTCAAAAGATACTG AAATCGGCTGATGCCTCAACGGATGACGACGACGAAGGTTGGCTCTACACGGCGACAGCAGCAGCAACATTGGAAGTTGC AACAGCAGCAACTGCAGCACCGACTGCCGCAGCAGCAACATCGAGTGTGGATGGTCTGCAGTCCACGGCCGTCTCCTCGA CTACGGCCACGGGTGGACCACTCCCGCCCAGCGACGACTCTGATAAGGAGAACAAGGTGGCTCTGGTCAGCGCCACGACA ATCACGGCGGCAGCAACCGCAACTGCAACATCCATTGCAACACTGCACAGCAGCAACTACGACAGCAGCAGCGCCTGTTC CTCCTCCAATTCAAATTCGAATTCGAACTCCAACTCGAACGGCAGGCTCACGGAGACCTCAGCCACCTCGCGAGTAACCC AGCTACAGATGCAAATCCACTCGCAATCGCAGTCGCAGTCACAGATGGAGCTCCAAATCAATGGCAATGCCATCGACGGC AGACACATCATTAGCAACAACAATTGCTACAGCAGCATGCAACATCAGCCGCAAAACAACAACGAGGGTGAGGCAGCCGA GGATCTGGCCAAGATCAAGATGGGTGACGATGAAGCAGCTGCTGATATGGCAAATGGAAACGCCACCAAATCTCAGCAGA TGAGCAACGGTGTGTACAGTCGCGCGGACTCTTGCAACTTCACCGTTTGGGCGGCAGAAACTGTAGCCAGTTGCCACTTG CCCCCGAGATCGCCGGCCAAATCGGCCAAGTCCTCGAAATCCCAGGCCAGCAATGCCACCGTGTCTGGATCCACGCTTGT TTCCCCAGTCAAGGGCAAGGTCTCGCATGATTCAATCAAGCAGTTGGTGCTGGAAGCCGAGCATTTGGTGCGCGACGCCC AAGAGACCGCACTGAAGACGCCAACCAAGCAGAAACATTCCATCATTAAGATCTCGTCGACGGTCAAGAAGCGCGAGGTC ACTATGCCCCATCCCATCAAGCAGCGTGTGGAGGAATGGCTGGAGCACCAGCCCTCGACCCCACAGCTGCTGACCCGGAG CCACACGCACGAGCTCCTGCCCAGCTGCAAGCCAGATGACTGTGAGGCCTCCGGAGAAGCATCCGAAACAGACTCGGTGC CCCAAGCTGGAGCAGGAGTAAACGGAGGAGCACCGAATGGTGCGGGATCGGATACATCGGAGGGCTTCACCGATTCCATT GCCACATGTATGCAAACGAGCACGAATTCCTATGGGAATTCCACGGAGAGAATCGGTGGATCGGCTGAACCCATTGGACA GCCCGTCACGCCTTTGGGCTTTGGCAGCAGCAACCAGAGCCTGAATGTAAAGATTGTAAAGCGATCGCAAACGCGTCGCA AGTCGGAGAGACCATGGTCGGTCTCGTGTCTGTCCCAGTTGACTACGGATGCAGCCCAGTTAACCACGGCCCGGATAGTG GAAAACTCTCCGTCCGGTCTGGCCAGTCACTCGATCAGTGAGAGTGCCCTGGACTCGCTTTCGCCAGGACCAAGACCGCG GGCAGCTTCTTCCTCGGGAACGGGAAGCAATGCAGCCAAGAAGGCGGATAGCAAGGGCTCATTGAGGAGGAGGAAGGCCA GGAAGAAGCGCATTTCGGCCGCATCGGCTGGCAGGAAATCTGATTCAGGTTCCGAACTGGGTGGAGATCTCACCCAGACA CTGATGAAGTCCTGCGAGTCCATGTCCTCGCAGCAGCTGCAGGAGTTCACCAATGCCCTTCTGAGCATTCAAAAAGGCGC AGTAGTTGCTCCTTTGAGTCCCAAGGGCGACGTTTCTGGAGTACCTTCACTGGCCGATGGCGAGGGAGGGGAAACCCAGC TGATGCTACCCAAGTTCCGGGTGGGCTCCTTCACCACAGCTGGCCTATTGGCTACTGATACGAGGTTAGGCGCTTTAGCA GCCCTCTCAAACTACATGAACGAGGACGAACAACAAGCAGAACTCAGCACTGAGGACCATCATAGCAGCATCTCGGAGAC GGCGTGGGACAACTACCAAGAGAAATACAACTCGGAGAACTATTCCGAGGGCTTTGATTCAGATGCGGCCCGGCGTCTCT TGGAATTCGGCGACGACTATCGCAATTTCATCGACTCGCAATCGGACTGCTGCAGTTCTCTGTCGGCGGCCAACAATCTG GACTCGTTCTCGCCGCCGCGCATGGACTCACTGCAGAAGCACGAGCTGAAGACCCTCCATATCAACCAGGACACAATCAC CAGCACCGTGGACCACGCCCGCCGCCAGCGCGCCCTTGAACTGCAGTACGAGCGTCGTCGCAAGACCCTGGAAGTTCGAC GAAAGTCTTGCCAAGACATGGATGAGAGCCTGATGGCTAGTCCCCAGTCGGATCAACAGCAGCAGCAATTGCAGGTCACG CCTTCGTTGTCTGCCTCCGCCACCGCCCTAATGACCACGCCCAAGATTCAGTCGACCAGCCACCAGATTAGCCATCGCGC AGAATCTGTGGGACGCAAGTTGGATTTCGGCGGAATGAGCCACTCCGCTCAGTCCCTGCTGCGTCGCACCTCGGAGAGCG ATACTTCAACCAGGAGACGACGCACTGTGACGGCGGACGAAAGAAGACGCTCCTCCCGCAACCTGGAGAAGTGCATCAAA CTTATTCCAGCTACCACTTCCTCGTCGAGTGGCTCGGACTCCGAGGACGGAGAGCAGGAGATGCGATCCCTGCTGCAGCA GAGCCGCGACAGGCTGGACGACACCAGAGCCCTGAAGATCAGATGCCATCTGCTTAGACCAGAGGATTATAACGAGATCA TCAACACTTGCCGCGACAACATTCGCTGCCTGGAGGCCGTGCTGCGCGGTCCGCCCGGCACCGTGTTGTCCAACCACTGC GCTGGCCAGACAAAAGATCTGCTCGGCGCCTGGGAGGATCTACTTAGCTGGAGCGAGAATGCCTCCGCTGCCCGCAAATT GCAGCAGGAGATGAGTGTGTTGAAGAGCTCGCTGCAGCGCCTGGGAGACAAGCCAACTCCAGAGCTCCTCGATACGGAGC CGGCCATCCAAATAGCAGTGGAGGCGCTCAAGTTGGAGCAGACGCAACTGACCAGCTATAGGACCAACATGCTGCGTCTT AACGCCTCCGTCCACAGTTGGCTTACCAAGCAGGAGCGGCGACTGCAGAGCGCCTTGGAGGAACAGGAGCAGCAACAGGA GTCCGAACAACTCAAGCAGCAAAAACTTGTTGAAGAGGAGAAGGGAGCTGACGTTCAGAAGGAGCTGGCGTCTACCGGAG CAGTGGCCATCACGGTCACCGACAGCAATGGTAATCAGGTGGAGGCACTAGCCACAGGAGAAGCCTCCACCTCCACGCCA GCCTGGGATGTGCACACGCTGATGTCATCGGAGCAGGAGTTCCATAAGCATCTGAAGAACGAAGTGAGCGACATGTACAG TTCCTGGGATGAGGCGGATGCCAGAATCAACACGCAACTGGAAATGCTTACCAACTCGCTTATTGCCTGGAGGCAGTTGG AGTCCGGTTTGAGTGAGTTCCAATTGGCTTTGGGCCAAGATAGGGGCACTCTGAAGGGTCTTGAAGGAGCTCTGGACAAG GGACAAGCCACACCAGTCGAGCTGGCCCAGAATGTAAAACTGGTGGCCAAGCTGCTGTCCGAAAAGGTTCACGTAAGCCA GGAGCAACTTTTGGCCGTCCAGCAGCACCTGGACCCCAACCACATCTACCACATTACGAAGTTTACAGCCTCAAATGGCT CGCTATCAGATTCCGGCATCTCTGATGGAGGAGCTACTTCTGATGGTGGCCTGTCCGAGAGGGAGCGACGACTGGGAGTT CTTCGACGCCTAGCAAAGCAGTTGGAACTAGCTCTGGCGCCGGGAAGCGAAGCCATGCGTTCTATTGCTGCCCGAATGGA AAGTGCCGAGGCCGACCTGAAGCACCTGCAGAACACCTGTAGAGACTTAATTGTTCGCACTGCAGCTAGCCACCAGCAGA AGCAGCAGATCCAGCAAAATCAGACCCAGCAGGTGTCACCGAAGGCCAATGGACACATTAAGAAGCAGGCCGCAAAGGGC AAGGCAGAACCCCAGTCGCCTGGCAGACGTGGTAAAGGAGCTCGAAAGGCGCGTCAGGCCAAGAAGGCTGGAGAGGATCA GCAAGTAGAGGAGCCAAGTCTCAGCCCAGAACAGCAGAAGATGGTCCTGAAGCAACTCAAGACCTTGACAAGTGGCGATG GTGGGGACGACCCCTCTGACGATCCCTCGTTGCTCTTCAACCTGGAAAGCTCCGAGGAAGATGGAGAGGGAGCGGATCCT GCACAGACCTCTAAGCGAGGGTGGGCGTGGCGCATCGCGAGGGCGGCAGTGCCTATGCAGGTGGCCCTGTTCACAATCTT CTGCGCTGCCTGCCTGATGCAACCCAACTGCTGCGACAATCTGAACAACCTGTCCATGAGCTTCACGCCGCAGCTCCGCT ATATCCGTGGACCACCTCCGATCTGAGGACAGAGCGCGACTAATTAAGCAGGCCGCTAATCGTTGTAGGTTACCGTTACG TTATGTTAAGGCATTTAACAGCTGTAACTAGAGTTAACCAGCGCAACCAGCCACTTAGGCAGAGCCACTAAGAGCTCGCC TGTGGCGCGCGTTTTCCGCGCTCGAGGTTTTACAATCCCGAACAGAGCGCTCTCCTCCGAAAGGTGCGACACCCAGAGGC AGCCCAATTTTATATGTCTAATTTAGGTAGCGAGCGCGAAAGGAACGCAATTTATAGTAAGGATGTATTTGTTATTTCGG TTAATTTCCTACGAGTTTAGGTTGCTGTTCGATTGAACGCTGAATATGTACTTAGGCGAGGAGCAGACGATGCCCAGCCA CGTGTAAACTGCCGCATAAAGGAACAAAGGGGCGGATCGTCCCCCAGATTCTTACCCAGTAAATATGATACAAACACAAT GATCAATTGAGTGGCGTTTTTGTATACTTTTTTGTTTGAAAAACACTAAAGTGCCTACAAATGCGAACTGTGAACGAGGA GCTACCAGCAGATGACAAAGGATCCGTCGAGGAGTCCAAACGAGAGTATGAATCCAAGGCACCGAAGCAAACAGAAGCTA AAAAGCCAACAGCACTAAGGAGCTAGCAATGGAGCTCACCAGAACAGCGATGAAGAATCAGAGTTTATATTTTTATACGC AACGCAACTATACACAATCAGTGCGGAGACAACGAATCTTCAGCCAACACACATCATACGTTTTATGTCTATTATATTAA TAGGATAAACCTTACACTTATTTTGCAAGAAAACAAAAATTCAACAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.