Comment: 0-22 hr AEL
CAGATGCAGACCGTGGTGAGTTGGCAACGCTACCAGCCAAGATCCGTGCCGCCGGAGAGATCAGTTGGTGGTCGTGCTTG AGACGGAGCGGTTGGCCGAGTGCGATCGCATTACTTTGCGAACGATTTATATATACATATAAACCGCGCTGCCCACAAAC GTGGCCAGATAAGAGGAGATATATACCAATACCTATACCTATTTCTCGCCGGCAGATTACTATAAATTAACAATAACAAG TGCAAACGTGTGAGAAAAATCAAAGAGGAAAAGCCAAAAAGACGGAGCACAAACATAAACCAAAATGCAAACGTGTAGAA GAAGAAAAGCCTCCGGCGGCCAATCCACGATCAAGTGGAGTAGAATGTGCCTGGCCACTCTCTGCGGATTACTTTTGCTT GGCATTCAAATTGAGCGTGCGGCGTCTGCGCCCGCAGGCGAAGACGCAGCGGCCACAACGATGCCACCTTTGGATACCAC AACAGATGCCCCAGACGCCGTTGCAGCCACCACCACTCCAGCCACAACTGCCGCAGAACAAAGCAGCAGCATCAGCAGCA TAACCACCGAGGCGGCGGATGGTTCAACGACTTCCACGACGACAACAACTGAGGCGGCCAACAAATCCAATGCGACCGAA ACTGATTTTACGACAAATGTGCCGGTGGCAAGCAGCCTGCCAGAGGAGACCAGCGTGCGATCGACGAGCATTGAACCCAT CACCTCCACGGAGCCCACGACAACGCCCCGCCAGGAAACGGAGGGACCCGATCAGCACATGGTCTTCTCCAACACGGAAC CAGATCAGAGCCACATTCAGCACATTCCGCTGCGGGATGAGCACGCCGAGAGCAGTGGCGCCGACGATGCCACCACCGAG ATGCAACGGCAGCGTGAGCAGGATCAGCAGCAGAATGAGCTTAATCAGATCTCTAATGAGCAGGACGATGTGGTCAAGGA TCTCAACAATTTCCGACATCCGGCCACGCTCATAACGGCCAGCAACAGCAACAGCGAGGAGAACGTCGAGATCGAAAGTG ACAAACAAGTTGAGACAACGACGACGGCGGTGCCGGCAGCAGCAACCTCCACATCCACAGAGGCAACAGGTACACCGCCA ACAGGTACACCAGCAACATCCACATCCACAGTCCCGAACGAACGCGAAGAAGATCCCTATCATGTGCATATACTGTCCGA GAATCATGATCGCCTGGCCGAACACGAAGATTATCAAATGCTCTCGACCAGCACCGAGGAATCGTCAACAACCTCTACCA CTTCGACTACTAACAGCACCACAGAGTCGGGCATTGTGGCTGGTATTGTTGTCAGTCAGGAGAACAAGGCAACCGCTGAG CCATCAACTGCAACCGAGTCTACATCCATATCCACATCCACAACAACTGCAGCAACAGCCGCAACTTCAACCACATCGCG AGCACGCGCCATGCATATGAATGATCCAGAAGATGAAGCGGCCACCACAATAATGCCGGACAGCGAGTCGGTGCCAGTGA TTAACATTGTTGAAGGACAACACATGCTGCAGCAGGAGGATCAGAAGGATGAGGAGGAGGAGGGGGTGGTCAAGGAGTCG GAGAGCAGTTCCACCACCGAGGCGTCGACCACCACCACCGAGCCATCGCCATTTGTGGCCTTTGCTGGCGAGGGACGATC GGCGGGTGGCGGCAATGATATCGAGCTGTTTCTGCACCACAATGGCTCTACACACGAGCAGCTCATGGATCTTAGTGATG TCAGCATGGACGGAGATCAGAACGAGGGCAGCAGCAAAACAGAGAGCAGCACTACTAGCACCACCACGACCACTGCTCAG CCGGAAACGGAAATGCCGAAAATTGTGGAGATCACTGCCAGCGGGGATACCATGCAGCGGGAATGCCTGGCCAACAACAA GAGCTATAAGCACGGCGAGTTGATGGAGCGGGATTGCGACGAGCGTTGCACCTGCAACCGCGGCGACTGGATGTGCGAGC CAAGGTGCAGGGGACTAAGTTATCCGCGCGGCAGCCAGCGCAGCATGGCCAATCCCAACTGTCTGGAGAAGATGGTGGAG GAGGACGAATGCTGTCGGGTGATGGAATGCAGTGAGCCGCAGCTGGAGCCCACGGTGGTAGCCACAGAGGGTGCTGCACC TTCCACCAATGGAACGGGAGAATCGGCTGTGACCCTGCCCACGACCGATGATGAAGCCACGCCCAAGCCCCGAACTGATT GCCACTACAACAGCGGTGTCTACAAATTCCGGGAGCGTCTGGAGATCGGGTGCGAGCAGATCTGTCATTGTGCCGAAGGA GGCGTCATGGATTGCAGGCCACGCTGCCCGGAACGGAATCACACGCGTCTGGACAAGTGTGTGTATGTAAAGGACCCGAA GGACGTGTGCTGCCAACTGGAGCTCTGCGATGTCACGCTGGACGATCACGAACAGCAGCCAACGCCGCTGCAGAGCAACA ACAACGAAGATCCAGAGGAGATCGACCCCTTCCGCTTCCAGGAGCAGGCTCGCGACGCCGGAGGCGCCAAGCCCACTTGC ACATTCAAGGGCGCAGAATATGATGTGGGTCAGCAGTTCCGCGATGGCTGCGACCAGTTGTGCATTTGCAATGAGCAGGG CATTCACTGTGCCAAGCTGGAGTGCCCCTCGAACTTTGGCCTGGATGTCCAGGATCCGCACTGCATACGCTGGGAACCGG TTCCGGCGGACTTCAAGCCCTCGCCGCCGAATTGCTGTCCGGAGAGCATGCGTTGCGTCGACAATGGCACATGCAGTTAC CAAGGCGTCCAGATCGAGAACTGGTCCCCTGTCCCAGCCAACCTGACAGGTTGCGATCAGCATTGTTATTGCGAGAATGG ACGGGTAGAGTGCAGGGCAGCTTGTCCTCCGGTTCCAGCTCTTCCTCCGGCGGACTTGCCCTGCCATCCAGCCTTGGCCC GCCTGCTGCCCATTCCCGATGACGAGTGCTGCAAGCACTGGATGTGTGCCCCCCAAATCCCGAAAATCGGAGGTGCGGGT CAGGACGAAGAGACGGAAGCTACTTCAACCCATTCCTCGATTCCAGCAAATGAAACAACAACAACGACAGCGACAGCAAA TAAATCGACCAGTATACCTAGCAAAGTACCCCAAATCAAGAAGGACGAGGAGAAGAGACCACCAGCAAGTGGCGCCTTCT ATCCAACCTTGGATGGCAAGCCACCCAAGTCGATTGGTGGTCTTGGTATCTTCGAGAAGCCGGAAAAACCAGAGAAGGCC CACAAGAAAGTGCAACATCAACAGCAGCAGCATCAGCAGCAGGAGCAGCAGGAGCAGCAGCAGCACCAGAATGATGTTAT ATTCGACGGTGATCGCACAGAGGAGCAGGAGGAGCCTTTGCCACCGAACGGCGGTTTTGTGCCCTTCCAATTCGGCCAGC AGCATCCGCATCAGCCACATCTTGGTCCGTATGGCTTCTACAATCCCGTGAAGCCCGTTTACGAGGACTATAATCCCTAT GAGCCGTACGACATCAATCCCAATGGCACACCACAGGGCAAACCGCCTCCAGTGCCCACTAGTCAGTCCGATTTGTTCAA TATATTAGGTGCGGAACAACCAGGACACCCGGTTCATCCTGGCCATGGTGGACCCCCCCGCATTCATCCTGGGCAAACGC AAAAAGACAATCACAACCTGGGACCACAAGTTAGAATCGAACAGATACTGCAGCACCTGCAGCAAACTGTTCCAGGTGGA CCACCACCACCTCCGCCCCACCAGCAACACCAATCCCTGACACCGCAGCTGCATCCGCAGCAGCAACAAATTTCGCAGCA ACATCCTGGTCATTATGTGCCCATCGTGCACAGTGGAGTGCCGCCACCGCCACCAGGACATGGCATTGCCATTGTCGATG GGCAAACAGTGGCCTATGAGAGTTATCCTGTGATCCCGGGACTGGGAGTACCACAGCACCATCCCCAGCAGCACCAGACG ACCCCGCAGCAACACTTGCAGCAGACAATCCTGCCTAGCTCGAGCACCACCTCCGGACTCTCGACGCAGGCTAGTGAGCA CAGTCTGCACCAGAACCAGGGCAAGCTGGCCAAACAGCAGCAGTCAGGAGCCAACAACCTGCAACCTGATATCGAAGTTC ACACACTGGAGGCCATCGATCCTCGTTCCATTCGCATCGTCTTCACCGTTCCCCAGGTCTATGTGAACCTCCATGGACGC GTGGAGCTGCGCTACTCGAATGGACCCAGTAACGATACATCCACCTGGGAACAACAGATTTTTGCTCCGCCCGAGGACCT CATTGCCACATCCCAGATGGAGTTCGATCTGCCCAGTCTGGAACCAAACTCACTGTACAAGGTGAAGATCACCTTAATCC TTCGTGATCTAAACTCGCAGCCCACGAGCAGTATCTACACCGTGAAGACGCCACCTGAAAGGACCATCACTCCGCCACCT CCGTTCCCCGATTACAGGCCAGACTTCCAGGACATCTTTAAGAACGTTGAGGATCCAGAACTGACGGTCAGCGAAACGAA TGCCAGCTGGTTGCAGTTGACATGGAAGAAACTTGGAGACGACCAAATGGAATATGTGGACGGAGTTCAGCTGCGCTACA AGGAACTGACGGGCATGATCTACTCCTCAACGCCTCTGATCCATCGCACGTTGACCAGCTACACCATCCAGAACCTTCAG CCGGATACGGGCTACGAGATTGGGCTGTACTACATCCCATTGGCTGGACATGGAGCTGAATTGCGTGCCGGACACATGAT TAAGGTGCGAACTGCCCAGAAGGTGGACGTGTATGGCTTCGATGTGACCGTTAACGTAACCAAGGTGAAGACCCAGAGTG TCGAGATCTCATGGAACGGAGTGCCCTATCCGGAGGACAAGTTCGTGCACATTTATCGTGCCATCTACCAGAGCGACGCT GGCAAGGAGGACTCCAGCGTCTTCAAGGTGGCCAAGCGGGACAGCACCACTGGTACCCTGATCATGGATCTCAAACCAGG CACCAAGTACCGCCTCTGGCTGGAAATGTACCTGACCAACGGCAACACCAAGAAGAGCAACGTTGTCAACTTCATCACGA AGCCAGGTGGTCCAGCCACTCCCGGAAAGACTGGAAAACTCCTAACGGCGGGAACGGACCAACCCGTAGGCGATTACTAC GGCCCCCTTGTGGTGGTTTCTGTGATCGCCGCTCTGGCGATCATGTCTACTTTGGCCCTGCTACTTATTATCACCAGGAG ACGAGTTCATCAAACGGCATCCATTACGCCACCACGAAAAAGCGACGCTGCCTACGATAATCCCTCATACAAGGTGGAGA TCCAACAGGAGACTATGAATCTGTAAATGGCGCACAATGCCCTAAAAAGCAACTAGAGTTAAAGCAGATTCTTGTAAATA TTGAAGGAAATGGATGTGGAAGAATAACTCAGTGTCAGTAAAGGAAAATCCTGAATCAATCACATTAAATCAGATGGAGC AAGCAATTTTCTGAAACCGGCGCAATATCAAAAAATTTGTTCAAATCGAAATGGCCGCCATGTTGAATGTAGTGGTTAAG TAATCTAAAAGCACATACCTTTTGTTAAACAATTTGTGACTTGTGTAAATAGAGAGAGATTGAGACAGAAGCAGACACCC TATAGAAGAACCAATTGACAACAGCTCCACAACTCAAATTGGCCTCGCTGGGCGTATCGATTCTATTGGCGATTTCAAAA TGCATACGAGTTTAGTTAGTTTAACTTGTAAATTGTAGCTTTACACTAATCGTAATTAAAGGGCGCATCAGCATCAACAG AATCCGCAGCACCAGCAATACGATGCGTTCTTCAATTAACAGTCCATAAATATTTAAATTAGCTTTTAAAACGTACATAG ATATGCATATATGTATGTATGTGAGTATGGATAAATAAAGAAAAGATAATAAAACCCAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
