Comment: 0-22 hr AEL
AATCGTTAGCCCAGCTCTAAAATAGTGTTTCTCTTCTCTCGTGTAGAAAAACAGAGAAATTAAACTTTTTCGCGCATTTC GCCATTGCAAAAATTGAAATTCGCGATCGCGTTCGTTTACCCTGCAAATTGCACAACTACGCGCTCTCGATTGGCGCCGA TTTAGCGGAGAATCGCGAAATCAAAGAAATATTCGCAGCAAGAAGAAGAACGGAACGGCCAGTATAATCGTATTGTTGTT TGTGTTGGTGTTAGTTGTGTGTGTGTTTGTGGGTGTTGTTGTTGCTGCTGCTACTGCTTCTGGGATGATGCTGTAAAGTA AAAGTGGTGGCAAAAGCACTAAGCCCGTGGTGCGACGCAAAAAACACGCTGCAGTTAGCGGCCAGGCGGAAAATGATAAG AGTGATGGTGGCTTGAGCCCCGAGTAATCGCTAAAATATCGAGCACACAGTTCGTGCAAATCAAATTGCGCCAATAAGCA AAAGCAAACGCAAACATCGTTTGTTTTATTGCATTCCACGCACACACACACCTATATATATGCACACGCACGCAGTCGCA TACATATGCGCTGCAGCAGCAACAGAGATTTTTTTATTTGACCACACTCACTCACACACGCAACCACATGCATCCACGCA GCAAACGGATAACAACAAAAACAAAGAGAGCAGCCAAATGTTTCGCCTGAGTGACCACATCACCAAGGATTAGAGTCAGC AACACGGAGTTAGAGGATACCAGAAGCAGCAAAGGAACCACCGCTAGCGAAGATGTCGCGCTGGGGCAAGAACATCGTGG TGCCGCTGGACTCACTCTGCAAGGAGAAGGAGAACACCAACCGGCCCACTGTCGCCCGTTCCGTGGGCACCGTTGGCAAG TTGGGCAAGATGGGCTTCACCTCCACGCGCACCTACACCCTGCCCGCCATCCATCCTATGGCTGCGGCAGCGGCGGCTGC TGCAGCAGCCGCCAGTCCCTCCCAGAGTCCCGCCTCCACGCAGGATCACGATCCCAACGACCTGTCCGTTTCGGTTCCGG AGCCGCCGAAGCCGAAAAAGTTCTTCAAATCGAGGAATACAGCTCCGCCGGAGGTCATAGCTCAGATCATTCAGCAGCTA CCGCACTGTGGAGCCGGCGCATCACCCATGCGAGACCATTTCTCGTCGGCGGGTGCGGGTGCTGGTGGTCTCACGCCTAC TTCCGGTGCCCAGGAGGCGGGCGGAGTGAAGCTGAAGCCGGGCAAGGGCGCCAGTTCCGCAGAGCGGAAGCGCAAATCGC CGAAAAAGAAGGCAGCAACAACGTCAGCCTCCACGCCCTCGACGCCTGGTGCGTTCTACGGCGCCTCCGATCGAGATGGC GATGGGCTAAGCGATCCCGCCTCAGAGCAGCCGGAGCAACCGTCCAGTGCCTCGGGCAAGCAGAAGCAGAAGAAGCCGAA GGAGGAGAAGAAGCTGAAGCCAGAGGCGCCGCCTTCGCGGGTACTGGGACGCGCCCGCAAGGCTGTCAACTACCGCGAAG TGGACGAGGACGAGCGCTATCCCACGCCCACCAAGGATCTGATCATTCCCAAAGCGGGGCGCCAACCGGCTGAAGTGGCG GCTACGGCAACACTTGCCGCCGCTTCGTCGGAAGCCTTCATCAGTTCCACGTTTGGCAGCCCTGGATCGGAGCCGTCGTT ACCCCCGCCTACGTCAGCGCCAAGTGCATCTGCGTCCACCTCGTCCCAACTGCCCTCCGCATCAGGCAGCGCGTCAAATC CTCCCAGCGCCTCCCGCACGCCAGAACATCCTCCTATCGTGCTACGCATCTCCAAGGGCACTTCGCGGTTGGTCAGCACG GATAGCGAGGAGCCGCCGAGCAGCTCGCCCGCTCACCAGAACCAACTGAATCAACTTTCGGTCACGGAGGAGGAGCCAGC GGAGCGGTCCGGAGACGAAACAGTTCCTGCAAGTACGCCAAAAATCACAGTGAAGCCACTGAGGCCGCCCACAGCAGCAG ATTCCGTCGATGGATCATCTGCGGCAGTTGGAGGAGCATCAGCAGGCGATTCTTTCGAGGAACGCAAGTCCCAGTCACTA GAACCCAACGAGGACGAGGAGGAGGAAGAAGAGGAGGAGGACGAGGAGGAGGAACCGCCGGAGATCAACTACTGCACGGT AAAGATATCCCCGGACAAACCGCCGAAGGAGCGGCTCAAACTGATCATCAAGACGGACGTGATCCGCAACGCCATCGCCA AAGCCGCAGCAGCAGCCGAGTCCCGCAGTGAAAAGAAGTCGAGGAGCAAAAAGCACAAGCACAAGCAGCTGCTTGCCGCG GGATCTGGTGCCGCTCCAGCTTCTGGAGCCACCCCCGCCGAGATCAACTCCGAATTCAAGACACCCTCACCTCATTTGGC TCTCAGCGAGGCCAATAGTCAACAAGCACAGCATACACCATCTCATCTACATCAACTGCACCAGCTGCATCCGCAGCGAG GCTCCGCGGTCATATCACCAACCACTCGATCCGATCACGACTTCGACTCGCAGTCCTCGGTGCTGGGCAGCATCTCCTCG AAGGGCAACAGCACGCCGCAACTGTTAGCGCAGGCTGTGCAGGAGGATAGTTGTGTGATTCGCAGCAGGGGATCTAGTGT GATCACCAGTGATCTAGAGACGAGTCAGCACTCCTCGCTGGTGGCTCCCCCCTCGGACATTGAGTCACGACTGGAGTCCA TGATGATGACCATCGACGGAGCGGGAACGGGAGCAGCATCTGCAGTGCCGGAGACACCACTGCAGGAGGACATACTGGCT GTGCTGCGAGGGGAAGTGCCACGGCTAAATGGCAATACGGACCCGGAGCCAACCGAGGAGGAGGATCAACAGCAACAGCC GAAGAGGGCCACGCGTGGCAGGGGCAGAAAGGCCAATAACAATGTGGATGTAACCCCACCCGCCACGGAGACCAGAACCC GAGGTAGGGCCAAAGGAGCAGATGCGACCACGGCTGCCATATCGCCACCAACGGGCAAAAGAAACACGCGGGGCACCCGG GGCTCCAGAAAGGCCGAGCAGGAAGTCGACATGGAAGTGGACGAAACGGCGATGACGACGGTGCCAGCGAACGAGGAACA GCTGGAACAGGCCACACTTCCACCGAGGAGAGGTCGCAATGCTGCTGCCCGAGCCAATAACAATAATTTGGCAAGCGTTA ACAATAACATTAATAAAATAGCCGCCAATTTGTCAGCCAAGGCCGAGGCCAGCCGACTAGCAGAAGGCGGAGTCGCGGGT GGAGCGGCACGAAGCTATGGTCGGAAACGAAAGAACCAGCAGGTAACGCAGGTGCTACAGCAGGAGCCAGTGCCCGAAGA GCAGGAAACTCCTGATGCTGAGGAGGAGCAGCCCACACCCGCCAAGATTCCGCACACAGATCACAGGGAACATTCGCCAG ACCATGATCCGGATCCTGATCCGGATGAACTGTCGAACAACTCAAACAACTCCTCGTTGCAGCACGATGGGTCCTCCTCC TCGCCCCCACCCCGCGATTTCAAGTTTAAGGATAAGTTTAAGCGAACATTGACACTGGACACACAGGGCGCGGCGAACGC CGGAGCGGGAGGAGCAGCAGCGGCGGCGCCACCTGAGTCCTCTGGCGAACAGCGGGGCGCTGTCAAGCTGGTCATCTCAA AGAAGAAAGGCAGCATCTTCAAAAGCCGCGCTCTGGTGCCATCCGATCAAGCGGAACAGGCTACAGTGGCCAAGCGACAT CTGTACAAGCACAGTTGGGATGCTGCGCTAGAAGCGAATGGCGGTGGAACCAACAGCGATGCCAGCAATGCCTCGGCATC TGGCGTGGGCGTCGCTGGGGCGAAGGATCATCTGCATCATTTAGCGGCGGGCAAGTCCGATGGTGATTTCGGTGACAGTC CGTCGTCGAACAACAATGGCTCCTCCAGTGCGTGCAGCAGCGCATCCACGTTGCGCGGCGATAGCCCGGCCCTCGGAAAG ATCTCGCGACTGGCGGGAAAACAACAGTACCTGCCACCTCCACTAGTTCCGATGCCTTTGACCTGGATTTGGAACCAATT GCCGGAGAGCTTGACCTAGAGCGTAGTGCAGCTGGTGCTTCCGCTGGTGGAACAGGGGCAACGACAGGCGGAGGTGGAGC GACGGGCGGTGGCGGCCCCGTTCGGGTCGACCGCAAAACTAAGGACTACTATCCAGTGGTGCGTAACGTTAAGACGGCCC ATCAAATTCAGGAGATCGGCGAGTACCAGGAAATGGACGACGACGTCGAGTACATCCTGGACGCACTTCAGCCGCACAAT CCCCCGGCAACACGGTGTCTCTCCGCCCTTCAGCTAGCCGCCAAGTGTATGATGCCCGCCTTCCGGATGCACGTGCGTGC CCATGGGGTGGTCACCAAATTTTTCAAGGCATTATCCGACGCCAACAAGGACCTCAGCCTAGGCCTGTGCACCTCGGCCA TCATGTACATTCTTTCCCAGGAGGGCCTCAACATGGACCTGGATCGCGACTCCTTGGAGCTGATGATCAACCTTCTGGAG GCGGACGGCGTGGGTGGCAGCACGGAGACTGGGCACCCGGATAGAGCGGGCTACGACCGCAACAAGCAGAAGGTGCGCGA GTTGTGCGAGGAGATCAAGGCGCAGGGCAAGGGAACGCATCTCAACGTTGATTCTCTGACTGTGGGCACGCTGGCAATGG AAACGCTGCTATCGCTAACATCCAAGCGCGCGGGCGAGTGGTTTAAAGAGGATCTGCGCAAGCTGGGTGGCCTGGAGCAC ATTATCAAGACCATCTCGGACTTCTGCAGACCGGTGATTGCCTGCGACACGGAGATTGACTGGCAGCCGACGCTGCTGGA TAACATGCAAACGGTGGCGCGTTGTCTGCGAGTCCTCGAAAACGTGACGCAGCACAATGAGACGAACCAGCGCTACATGC TCACCTCTGGCCAGGGAAAAGCAGTGGAGACGCTTTGCCAACTGTACCGTCTTTGCAGCCGACAAATAATGCTGCATCCT TCGGATGGTGGTGGCAGCAACAAGGAGCATCCGGGTGTGGCTATGCGCGAGCTGCTGGTGCCGGTGCTCAAGGTACTGAT CAACCTGACGCACACGTTCAACGAGGCGCAGCCATCGTTGGGAGCCGAGCTGCTAGGTCAAAGGGGCGATGTGGTGGAGA CGAGCTTCCGATTGCTGCTGCTCTCGGCCAACTACATTCCCGACCAATGTGTCTTTGAGCTAAGCATACTGGTTCTTACA CTGCTAATCAATTTGTGCATGCATACTGTGCCCAACCGGGCTGCTCTAATGCAAGCTGCCGCTCCGGCAGAGTACGTAGC GGATAATCCACCAGCGCAGGGATCTGTGAGTGCACTGCAAGCTCTGCTTGAGTACTTCTACAAGTGCGAGGAGCTGGCTA GATTGGTGGAAAAGAACACGGACGCCTTCCTCGAGAGCAACGAGAAGGGAAAGAAGAAACAAGAAGAAGTGGAGGAGACA GTCAACAATCTTGTGCAACGAGCCGGCCACCACATGGAGCACACGCTAAAGGGAAGTTATGCGGCCATCCTGGTGGGAAA TCTGATAGCGGACAACGAGTTGTACGAGTCGGTGGTGCGCCGCCAGCTGCGAGGAAATAGCTTTAAGGAGATTATTGGAG TGCTGGAGAAGTACCACACATTCATGAACCTTACATCCAGCTTGGAGGCAGCCTTTGTGGCGCATATGAAGTCCACGAAG CGCATCATCGACAACTTTAAGAAGCGCGACTACATCTACGAGCACTCGGATGAGCACGACAACCCCCTGCCTCTGAATCT GGAAACGACGGCGCAAGTCTTGGCCGTGGGAGCGGACGCGTCGCATGCTGCTACAAGCTCGACCACGGTCGGCTCTGGCT CCGCACCCTCATCCACATCGGCCACAGGAACGACGAGGGCGCCGCGGGTCTATAAAACGTACAGCAGCCACAGGTAATCG GACCTGGGATCTGAATCGGAATCGAAGAGCCATCGCAATTGTCGTTAACCACACCGAAAGCCTGCCACCAAAATAAAATT CTGTTGTTTGTGATTATGTAATTTGTTTATGTGTTGATATAACAATCACTACATGTATGTGTATAGCCTGTAATGCACCT AGTTTCAGTCAGCTATATATATATAGATATATATATATATAGAAGTATATAGCTAAGAGCGATAGGATACAATGCGATCT GTTTTTGCGTTTGTATTAACCCAAAGTGATAGCGAATTCCTGAGCATGGTCTGCGTTTTAACTAAAGAAAATGATTAAGA TTGACGAAACGAAAGCGACACCCCGAACAACACAAAGATCCAAATCCCCACTAATATGAGTACGTTATATAGTCATAAGC AATATTAGCAGCTAAATGTCGGCTTACCCTCGAAAGCACACACCCATGTAAATATTCTTTAATCAGCGATTTTATCGGAC GCGTCTTAGGTTTATTTCGAATGTGTTTTTTTTTTACCTATTCATACTTTATATATTAAACCTTTTTTTTATTGATTGTA AAACAAGCGCTCTTAGCAAAAACGCTGCAGCAGAAAGAGCGAGAGCGATAGACATGTGTAAAGAGAGAGTTAGAGAGGAA AATTCTAGATTAGATGTGGGAAATAAAGTTATTTTATTGCCTAAACGTAAGCTAAAGAAACTCTACTTATAAAACTAACA CTGATAAATAAATATATATATGTATATGGATATATGTGTACGTTTACAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
