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FB2026_02
,
released June 18, 2026
Comment: 0-22 hr AEL
CACGATGGATTCTATTCTTTGCATGATTTTGCTGATTTTGGTAAGAAATCATGCTTCTGAGGCAGCTAGGTTGAACCACC CGCGTGTGTTACTACCAATATTCGAAGACAAGGCAATAAACTTTACCCTGGAAGTCGACGAGCCGAACTGCTACAAATGG TCGTCCTCACGCCAGGACTTGATATCTGTCATGCCCATTTACAAGGGCTTCAGCGAATGTGCATACCAGGCGGTGGTGAC AGTCCGCACTCACGATCGTCGGAGGAACACTGCAATCGTCTTCGCCGAGGAAGTACAAACGGGCGAGACGCTGCGTTCCG ACGTGATTGTGGATGTCATTGCTAGTCTAAATGTGCGCACCGCCACTCGACAACTTTACCTGGAGGAGGCGCCCGCCATG TTCGAGCTGCACGCCTTTGATGAACAAGGCAACGAGTTTTTTACCTTGGAGGGAATCGAGTTTGACTGGGAGATTTTGGA ACCCGGTAGCAAAAGGCCCACCGCAATGCGATACCTCACGTTCACGGACAGTCCGTACCACACTGTACCGCCCACCATTG AAAAGTTTGAAGCCGACGGCAAGAAGGGGCACATGATCCTCCTTGAGGGCATTAATACTGGCACCGCCAAGGTGACCATC GCCATGCCTCAGGCCGAGTACAAGCATGTGCGGCCAGTTGAGGTGTACATCAGCGTTCTTGCAAACATTATAATTGAGCC TTCCGAAGTCACCATAATGGCTGGAGACTCCGTCTCATTTCGCATTCTGCAGCTGAAGATGGACCGACTGCACGTTATAG ACAACAATCAGTATTATTTGGAGGTGGAGGACTCCAGCATTGCATACCTTCGTGGAAACAGTGCCACCGGAGCGGCTCTG GGTCGCACCCAGGTCTTTCTGCGAGACCGCAACATGGCGGACTCTGATGAGGTCCAAAAGGGACCCAGCGCTCTATTGAC TGTGGCATATCCGAATAGGTTGTCCATCAGTCTGTTGCCCCATCTCAATTGGGTGACAGTACAAGGCGAGCATCACGCTA TAGCATTGGATCTTTTTGCTGCCGACGGTCAGAAGATCACAATGGGCACTAAATATTCAATCAACTCAGAGGTGGATGAA TCCTTTTTCGCCATTGTTGATCGTACTCGCAACGGAAGCCGCCTTTTCGGTCAAGCAAAAAAAGAGGGAATCACTCAGGT GTATGGCTCATATAAAGACCTGTCGGTACAAGCTGAGCTTCAGATATTTGAGGAGCTTCAGTTAGCGCCAACCAAGGTGG TGTTGCCATACGATCCTAACAGCTTAAAACCTTTAAAGCTGCAGTTTCATGCATCTGGCGGAGATAACAACTACGCTTGG TTCTCCGGAAATCCACAGGTGATCCAGATTGACACCCAGGGCCAAGCCACAACTGAAATCCGCGACGTGAAATCCGCTTA CGTGAACCAGGAGGTACTGAAAGATGGTGGTAAACTGACCGCCCACACCACCGTCAAGGTGGCTCTATCCAAGAACCAGA AAATTTCCCGTGTGGCACATATCTACTTTCTCCCCCCTGAGCGCCTTCAGATCACGCGGAGCAACTTTGAGACCGCCCTG AAGGACTTCGTACACGTGCATGTGGGCGTATACGCTCGAATAAATAATTCAGAGGTTCCCTACACAAGTTGTGATAATCT TCACTTCCAACTAGACTTCAGCCAGCCCATTCTGCAGTTGGAGGGTAATGAAGGGGCGGAGGCTGCTCACGAGGCGTGTC ATGTGCTTCGGTTGCGGGCCACCGCTGTGGGCACCACCTCGCTACGCGTTTCCTATATGTATATGGACAAAGTGCTTTAC GACATTATTGATTTGTATGTCTTTGAGCCATTAGTCGTGCTAAATCCAATAGAAAACGAGGTTGTTCTTCCCGTTGGGTC ATCCCGTAATATAATTTATGCTAATGGGCCGCAGCGCAGTTTTACGGTGGCAGCAGAAATCATACAATCTACCGCTTTTG ATGAAAAAATATTAAAAGTGTCTAAATTAGAGTTCGACACGCAAAATTTGATAACTGCGTTCACCGTGCTGTGTCGTGAG CTGGGCGAGACGCAGTTCACTTACCGAGTGCATAATAGCCTTCCCACCTCAAGCTTTGCCTTATATCAATCGGAGGTGAC CACAAAGGTGCATTGCGTCCGTCCTCGCTTCCTAAAGCTCTACGCTCGCCATAACCTGCGTGACTCCTGCCCCCTGGAGA AGCGCACCTCGTTATTATTCCTGAAAGATCCAGAGAATAAAATTGAAATTGAAATCGAGGTACACGATTCAAATAATCGT AGGCTCATGAATATCAGCTCACTAGGGCTTGACTGGGAGTTTTCCGCTGGTGAAGAGCGATACCAGAAGAACATTATCCC GCACCGTCAGATTTCTGAATTGGAGTTCAATCATGGAGTTACCCTGCCGAGTCGCGACCTTCTTGTCTTGACATTGAGTG AAGTGGCGACCAACTTCCGCATTAAGGGAACGGTGTCCCAGTATAACGACAAGCTACTTGCCCAACACGGTATTCACGCG GAGCGTCCTCCCTTTGGAATCAAAAATCCTCAGACGGGTTTAATATACACTCCTCTGATTGAAAATGAAATTAGGTTACA CGCCGTTAACAGTACGTTGTTGCCAAAAGATTACATGAGCATTTTTCTGGCCTCGGGCTATAGCGAGCGAATACCAATCG CCCAGGGCAGCGGCTACTTGCAGCTGGAGCTCTCCGAGGCAGGTATTGTTCAAGTTGAATACAATGAAAATACCCGAATC CTAGTGCTGACGCCACTACGCCTTGGCCATGTTCGTCTTGAGTTGACCGACCGTTGCTTAATGAACGAACCCAGCCATTT ATCCATCTCTGTGGTTGGAATCGGGGCTATTGAGGTAGTTAGCATGGACCGGCTAGAACGCACTACTAGAATCGAAGCTA TCGTAAGGCTTTTTGATACCAATGATAATCTCTTGCTTGTGGACCAGAGCAAACTTAGTGCGTACGATTTATCCGAGGTT GTCGCAGACCAAAGTATACTGAGCGTGCGCCTAGGCGAGCAGGAAAATGTAGGACCCGGAGAGATCCGCTACACAATTAC GGGCAACCAAGTCGGCGAAACCAAAATATTATTTCAGTCTGGCAAGGGCATTTATAAGGTGGCAAGCGACCCTCTAAATA TTCAAGTTTTCGCTCCAATTCGTCTCTTTCCGCGCGACTCAACTCTAGTGGTGGGCAGCAGTATACAGGTGTACTTCCAT GGAGGACCGCACCCAAATACCAACATGATAATCTCAGTGGAGAAGGAGCAAGTAGCAACTATAAGCTCTACTGTTGTTAC GGCCCATAAGCTGGGGACCACCAAAATCGTAGGCAAATGTCTTCTAAAAAACCCTGTCACTGGAAAGGACGAGGTGGTCT CCCAGGACTCGGTGGAGGTACACGTAGTGGCTCTGAAAGGAGTACAGATTCGAACGCCTTTGGTCCGCATTCATAGTGGG GCCGTAATGCCAGCTACCTTGTGGGGTCTTTCTGATTTGTCGCCCATGATTTTGGGTACCTTGCAAAACACTAAAATCTC CTGGAAAGTAAGCCAACCACAGGTGGTTGAGATATTTAATGTATTTACAACTGCAGGAATAGAGTATCAGAGTGGGGATT TGATATCAGTTAGAGTAAGAGCCCTCAATCCTGGAAAGGCAACAATCACGGCATCGGTTACTTTGGCAGACGGAACAATA CTGCCACCAGCTACAGTGGACTTACAAGTTTTTAAAACATTGGAGCTGGTAACACCAAATGCCATTAAAATGGATTCAAT TTTGGCTGCGCCGCGAAGCATTCTTCAGCTTAAATCTAATATGGATAATGTGGTCTATAAACTGGACGATCGAAGTAATG GCATTGTCAGTGTAACACCAGATGGCCTTGTACACACAAAGGACTCTCTTGGCCGTGATCTGATAATTGCGACGACCGCG GACCAATCCCTACCCATAGGAATTGAAGTTAAAAATGTGCAATACATTTTAGTAACGCTGATGCCAATCCTCAAATTAAG AGAGCTTGAACACAAAATCCCGCGTGGAATGAACTTTGTGTTTAAAGTGTCGCTTCACGACAATTTAGGCAACGAGCTGT CGCACAACATCGAGGATTTCAACGGACTACGATATGAACTCGGCAACAAAGATGATGTGGATGTCCAGATAGATAATAAT CTAACGTTTGCGTTGAACTTGATGCGAGAGACCAACAATGTCATTGGCATTAGTCTAAAGGACTCAACGGGTGTCAAGCA CTCCATGGACTTCATAAAGCTATCTGTTGTCGAGTCGGACAATTTATTTCCCACCAAGACTATTTTCTCTGTTGGCGACA TAATTTGTTTCGATTCACCTTTAACGTTGTCATCGACTTGGCGAAGCTCCAATGAGCAGATAGTTTACATTAATAAGCAT ACAGGCATTGCCCAGGTGTTAAGTCATCGACTAAAGCCGGGAGAAAAGATTGAGATCACTAATGGCGACGAGACAAAACG CGGCGGATTTTTAAAGTATGACCTGGAAGTTCGCGAATCGGATACGATTTTGTTTGTCAAATCAGTGGACACATTTAGCG GACCAGAATATCGGGGACAACTAGTTATTCGCAACCATTTGCAGTCCGAAAAGTATAGTAACCTTATTGCACAAAATGTT TCAAAATGTGCCAGGGAATTGGGAAGCGTACCGGTGAATTTCTTTACATGTCGCCTGGCGGCCAAGGATGCTCTGGGACG TAATTTACTAAAAATGTATAAAGTTGATGCATTGTTTGAGCCTAGCATCGGGCAGTACTCTTGTCGACTACAGTTGCTCA CCGGTTTTATTGAGTTGCTAAGTATTGTCAAGACACACGACGTCTACCTGGAGCTGGAGGCAGTGGTGGCCAAAGGAGTT TCCGATAAAATGTCATTGAAATTAGTACCCGGTATCAAAGTTTTTCCTGAATCAGTGCGTGTCACCGACCTAAAGCCACA TGAAATTCATATAAGCGGACTAGACAAGGCGTTGCTCAAGGTTCAGGTGAAGCCATCGGATTCAAAATACTTTGCTGTGG ACTTTATCGAGCATGGACATGGTTTAAGCAAATATCGTTTGGAACTGTTTGATGACCTTCCGCTGGATGAAAACTTTTAC ATTTTAGTAGTTTCACCGGACACCAAACAAAGCATCGAGGTGCCCATTATCGGCAATACCATGCTGGCCCCGAAGTGCAC TGACCGCCGATATGGCGGTCCTCTTGTGTATCGGATACTGGAGAATTTGGGCTTCGTCCTGACTACGACTGTTATTGTAA TCATTTCCATATGGGTATACATGTCTTGCTTTCAAACTCAGGGCGTCACGCAAGTGAATTTTGAAGCATTCAAGAAAGGG AAGTCGAGGACGGAGTTGATGCAACAATCTGGAAGAAGCAGTCAAGACGATACATTTGGTGACTCCTTTAACGTACGTAA CTTTTCTCCGGATCGGCGAAGACCCCCATCGAATGCTTTGTCGGAGTCGTATATTTATGGACACCCTCGTTTGAACTCTT CAAACAGATCAGAAAACTCGACTAGCTTTAGTTAGTTGGATACGTAAATTAAAGACTGTCAGCAAACTGACTCGGGTTTT TTTGTAAAATAATATAAGATAAACTCAGGCATTTGTAATACTTATATGCAGCTTCTGTCCTTTAAAGTAAATTCTTCTCA TCCTGACGCCAGAAAAATAGTTTGTTAGGATAAGTCAATATTTGTTATGGAATAGAAGAATTAAAAAAAGAGTGAGATTG CAACGTCACAAGTTTGTCAGATAAGCTTACGTTTGTTCTACGTTTCCGCAAAACACAACTAGTTTTTAAATAAACATCAA TTAAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.