FB2026_02 , released June 18, 2026
Clone: Dmel\LD46794
Some tools are temporarily unavailable. We are completing maintenance and working to restore these tools as soon as possible. Thank you for your patience.
Open Close
General Information
Symbol
Dmel\LD46794
Species
D. melanogaster
Name
LD46794
FlyBase ID
FBcl0175723
Feature type
cDNA_clone
Computed gene(s)
Dmel\RhoBTB
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    LD_pOT2_cDNA
    • LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pOT2 vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-22 hr AEL

      (Rubin et al., 2000)
      Sequence Data of the Insert
      5' Sequence
      Total bases
      576
      NCBI
      AI515105
      5' sequence dataSequence Downloader
      GCCGTCAAAGCGCGTAGAAGAATTGCGGGCTGTAACTGCAATTTATTATTTTTTTAATTTCATTTATAGTGCACCTGGTC
AGCGAGCACTGGCTCATCCGCAGCCATCCGTCGGCAACCAGCTCGCAGCGATGTTAAACTTGTGCCAGCCGTAAAGATTT
CGCGTGTTTGCGAACCACAAACTGCGTCGCCATCGGTGTGTGGGTGCAGAAATAAATGCAAATTGTTGTAAAATGCCGTG
CTTTGGTGTGCGTGTTGATAAGTGCAATACGCAAAAGTAAGAACATTGAGGCAAATCCGTGCAATTCGTGCAATCCGTGG
GATCAGGCGTTTTCAGAGATTCCATAAGCTGGTTCTGCTATTTGTGCATGCCCAAAATGGACAACGAGCAGCCGCATCAA
GAGCTAGTGAAGTGCGTCCTGGTGGGGGACACGGCCGTGGGCAAGACGCGACTGATCTGCGCGAGGGCCTGCAACAAACA
CGTCTCGCTATCTCAGCTCCTCTCTACGCACGTGCCGACCGTGTGGGCCATTGACCAGTATCGCATATATAAGGATGTAC
TCGAGCGATCATGGGA
      Library Information (1)
      Library: LD_pOT2_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.

      (Rubin et al., 2000)
      Sample preparation

      Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

      (Rubin et al., 2000)
      Comment
      Reported As
      Symbol Synonyms
      LD
      (Rubin et al., 2000)
      LD_pOT2
      (Rubin et al., 2000)
      LD_pOT2_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)