Comment: 0-22 hr AEL
TCCACTGCAAATTGGACGTGTTTAAAAAAGTACAGATATGTTTACAACGCGAAAAGAAGTGGACGCACACGTCCACAAAA TGTTGGGAAAACTTCAGCCGGGAAGAGAGCGCGATATCAAAGGCTTGGCTGTAGCTCGATTGTACATGAAAGTTCAGGAA TACCCAAAAGCCATCGAGTATTTGAACGGCTATCTCCGGGTGAGGGACGACGCTGTAGGCCATAACATGATTGCCACTTG CTACAGTAGGCTGAACCCGCCCGATGTGACAGAGGCCCTGCAGCACTACCAACGTTCTATTCAAATTGATCCGCGTCAGT CAGAGGTTGTCATTGACGCCTGCGAACTTCTGGTGAAGGAGAACAACGCATCGATCACGGAGTGCGCCAGGTACTGGTTG GATCAGGCTAATAGCCTTGATTTAAGCGGCAACAAGCAAGTCTTCAACCTGCGAATGCGTGTAAATCTTGCGGACAGTAA CGGCGAAAGAGATGACACCAGTGGCGGAGACGGCGAGCAAAATACCCTCGAGATTCTTATGTACAAGGAGCTGCAGGCGC GTCCCCAGGATGTTAATATACGCATACAACTGCTGCGCAGCTATGTTGAAAAAATGAAAATAGACCAGGCCTTCAACTAT GCTCTAAAAACGGAACTGGAGTCGAAAAACTGCACGAGCCAGTCGAACGAATGGTACGAGCAGATCTGGATGGTCCTTTT CAAGATAGAAATGGCCAAGGATGTAAAGAAGAACTGGCGATTTTGGCACTTCGCCTTGCACACTCTCGATCGTCTCGTGC AGCTAAGCCTGGAGGGCAGTGGACTGGCAGACAGCAGCAAGCAGTTGTTCAGACTTGATCAATATCTATTCAAGTTTAGC ACTTCGATAGAGAGGTCAGGGGACGCTCCGCAGCGGGATCTCCACCAGGCTTGTATTGACCACTTTACCGGCCAGCTTCT GCTTCACGCGGTGACACTGATTTTCAAACGTGAGGTTCTGGCAAACAAAAACAAATGGATGAGCACGCTTCGCTCCGCTT TGCCACTTCTGCTCCTTGGTTACCAGGTGCGACCGATCGATGATTCGAGTACCAACCAATGGATAAAACACTGCGACGCG GAACAGAAGCAGTTGATACAGATGTGGCGCCCTCAGGGCGCCTTTCGATGTGCCCAGCTTGGACGAACCCTTTTGGGCTG CTTGGACCGGTCACAAATGGAAATTAAAAACGATAGAGAAAATGCCGAGTTTGATGAGAATAAGAATTCGGGAAATTCTA TGCCGGGATTATTTGCCGACTCCGAAGAGCTGTTGGCCAGCGCTCACCAGCAATGCTTGGACAAGAGCTGGCGAAGTCAA ATTTACCAACAGCTCTTTACCCACGCCGAACACAAGCTAAAGGATACGTCTTCGCATCTGGTGCGGAACCTGCGTCTGCA GCTGCCTCTTTTTGAGTGGCCGAATCTGGCGCATATCGAGAACTACGAACTGCAAGCGTTGGTGCTGCCGCCACACTCCT TAGCCCAACATGTCTACCTGGCTCTGGGCACCGACCCGAACAAACTTGGAGATGCGCCACGCGTGGTCTTTTATGAGGGT TTCCAACGGGATGTTAAGCAGAATCTAAACTACTGTGGGCAAGATTCAATCAGCCAGGTAGATGTGGACCTCTACCTTTA TGCCACAACCATTCAGACCAGACGTAAATTGCAAATCCAGCGTGAGGTGTATGACTCCTCCAATTTGGGAAACCGCAATG CTGCCGCCCGGCCACATATGATGCCATTTGCCAATCTAGTGGGTCAACTAGGCGCACCGGAGCAAAGTAACTGGTGGGAT CTGGTGGTGCGACTTAACTCGAACCAGCTTATCACTGAAGGCAATCGCGCCGAGCAACGAGCTCAACTACAACATGGATT GGAAGCGGTGCGCGGCGTTAATGGCCCAAAAGCAGATGCCATCATCATTTTTCAATTGGGAAAGATTTTAAATTCCCGAT CGGATCGTTCTTCTCTAGAAGCGCGTATCGATACCCTGTACAGGCAAGGATTTTCAATTTTACGCCACCAACACAACCAG CAGATGGAATCCTATGTCCGCGTCTTTAAGTACGGATCCGCCGGCTCAACGGCAGCATGGCAAGATCTTCAGTCGTTGGC TGAGGAAGCCGTCACCTATTTTAGCGAGAAAATGTTTAGGATTGGACAGTATGAGCAGTTTTTGGACGAGGTCCGGGGAC TTCATCTGCCCATGGCCTACTTTTTGCAATCGGAGGCGTGTCATCATCTTGAGGAATCTTCCAAGCTGCCTCGAACTTCC CGAGATCGTTACTCAGAGCGAAGACGTGAATGCCTGCAGAAAACGCAAAAGTTAATCAAAAATGATGATAAGCACCCACT CATAGCGGCCATGCATAGACATCAACAGGACCGCAATAGTCGGGGTATCGATAATAGCTTCGGCAGTCCGGATGTTCATA ACAATTCATCCGCCTACGAGGACGCAGAAGATGATTTCTATTCCCATGCAGCCTTCTCTGCCAATCGATCACGTCGTCAG TTAGAAGTCACCCCAGTCACTCCCATTGTTATGGCACAACCGAGTCAGGAAATGGAGCAGGCTGTCAAGCAGATCAGCAA GTCACTGTGTGTGCTCAAGGACGATGTGAGTGTGGGAATGGAAGCCATGCGGCAGGATATCAAAGTTCTTACTGAGAAGT TCACTGGCCTGGAGGATCTGCTTAAGAAGATCAAGATCAGCAGTCGAGACACGCCTACACGAGATGTCGATCCCGCAGCT GCCTTAGGCCTGGACGACCTCTTCATAATTGAGGATGCACTTGCGGAGCATCAGCAGCAGCAACAGCATCAGCAGCAGCA GAGCCACAACCAAGGTGCAATACACCCAGTCGTACCAAATCCATATACTTCTGGCTTCTACAACGGGATGCCAAACACGC CATCCGCCCAAGAGCGCTTTCTGCAGGGACCATACGGGAGTCCCATGTTTAACCAAAACCAGATGTACAACTACTACGCT GCCCAAGCTCAGGCTCAAGCGCAAGCGCAGTTCTTGCGGACTCCTCCAGCTCCAGGATCAATTCCACCTCCGAATATGTT TGGTCCTCGAAATCCAAACTTTGGCCTGCCCAGTATGTTTCCCCCGCCGACCGTGCCTTCGGTAGCACCTTACATTGACG CCATGGGCAACTTTACCCAGCCTCCCCCAAGTTTGATACCTCCTCCCGCTCAGCCAGCAGCTCCTCCGGCTCCCTTGAAT ATTCTTGAGTCCAAGCCTGTGGTTGCACTGCCAACTCCAGGATTCTTCAATACGACAACACCGGTCTTTGGAGCTTCGCC CATTCAAGTTCCGCAGTCGAAACCATTAACTGTTCCAACGGTGCCAATACCTAGTACTGCTCCTGCTCCACCAATTGCTG GAACTGTGAATCCACCGGCAACAACTGCTGTTCCGCCTCCTGTTCATATACCTCAAGTGGCTCCTTCTGTTCCTGCACAG CCACCCGCACCCGCCCCTGTTAGTGTGCCGTCTATGTTTAACCGTGCGTTGAACAATCAGCCCGTGGAAAAGGAGCCGCC AGCCAATGTGGTCATAACCAGTTCAGATCCGTTGCCGAAACCCACGACTGCCAGTGTCCAACCCACTTTAAGTGTGACCA TTCCAGCACAGCATATCAAGCCAAGTTTGGTTCAAGCACCGGAACAGCCGGCACAATCGGCACAACCGGCACAGCCATCG GTGTCAGGAGTAGGATCACTCAGCTTTAACTTTGGCAGTAAGTCTTCCGAAAGTCCTTTTTCATTCAAGACACAGGTGGC TAAGGCAGCAGCCGAAAAACAAAAGGAACAAGAGGAAGCTGAGCAAAACCAAAGTGGGGCTACTGATCCCAACAAAACTC TGCCACAAGATACTTCAGCAGATGATTACGATCCTCGTCCCGATTTCAAGCCCATCATTCCACTACCCGATGAAGTGGAG GTTCGAACAGGGGAGGAGGGCGAGGATATCAAGTTTACCAGCCGAGCCAAGCTCTTTCGATATGTGGACAAGGAGTGGAA GGAGCGCGGGACAGGGGTGATCAAAATTTTATGCGACAAAGCCACCGGCGTTTCGCGAGTCTTGATGCGTCGCGATCAAA CTCACAAAGTGTGTGCTAATCACACAATCACTGCAGACATTACGATAAATGTTGCCAACCAGGACAAGGATAAGAAATCC CTTCTGTGGGCGGCAAATGATTTTGCGGATGAGCAAGTTACATTGGAGAGATTCCTGGTGCGTTTCAAAACAGGGGAGTT GGCTGAAGAGTTCAGGGTGGCTTTTACAAAGGCAAGCGAGGCCGCCAAGTCGAAGGAAACCGTGAAACCGACGGTAAACA CAGCGGAGAAAGGATCAACTGCGACTGCTCCAGCGGCATTCAAGAGCTTTGTAACCAGCACCCCAGCAGCCAATTCGCTA ATCAATAAGCCTCAGGAGCAGACAAAAACTCAGCCTAATCCGGACCCACCAGCAACAGCAGCTAAGTCACTGTTTGGAAC CCTGTCAGTCAGTGCTGCTCCTGCAACATCTGCGCCAGCCTCTGCCACACCATTTGCCAGCTTTAGCTTCACGCCGAATG GGTCATCAGGATTTGGAACTTCCACCGCCTCTCCGTTCGGAAACCTTTCTTTTGGTACCGCTTCTGCGGTGGGCAGCGGC AACAACACTACTTTATTCACCACGGCTTTGATCAAGGACAATACAGTTCAAGGCAAAACGCTTCAGCAGGAGTCACAATT AAACAAGTCAAACTCCTCGGACGCTGAGGAGGAGTATGTGCCCACCGCTCAGTTCGTGCCTGTTATTGCCCTGCCCGATA TAGTGGAGGTGGTCACCGGCGAAGAGAATGAGGATGTGCTCTTCGAGCACCGTGCCAAGCTTCTGCGTTGGGATAAGGAA GCGAATGAGTGGAAGGAGCGCGGCCTTGGTAATATGAAGCTCCTTCGGGATCGCACTGATCCCAACAAGGTTCGCCTGCT TATGCGGCGCGAGCAGGTCCATAAGCTGTGCTGCAATCAGCGACTGTTGCCTGAGACCAAGTTCACTTATGCAACGAACT GTAAAGCGGCCGTCACATGGGGTGCTCAGGATTATTCCGATGAGGAGTTAACCACCGCATTGCTCGCTGTGCGATTTAAG TCGCAAGACATCTGTCAGCAATTC
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
