FB2026_02 , released June 18, 2026
Clone: Dmel\LD31049
Some tools are temporarily unavailable. We are completing maintenance and working to restore these tools as soon as possible. Thank you for your patience.
Open Close
General Information
Symbol
Dmel\LD31049
Species
D. melanogaster
Name
LD31049
FlyBase ID
FBcl0176593
Feature type
cDNA_clone
Computed gene(s)
Dmel\Usp7
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    LD_pOT2_cDNA
    • LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pOT2 vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-22 hr AEL

      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      4,568
      NCBI
      BT044531
      Full Length sequence dataSequence Downloader
      GCTAGCACACGCATACGGCGAAAAATACGCAAAGAATCTTCACTATTTGCCAAATTTTCAGTACGCAACGTTACATCTTT
GTTACATCGCCGATTTGATTGTCAATTGCCGTAAAATCAGCGTTTGTGCGTGAGAACAACCTTGCCCGCCCACGGAAATA
TCCTCCGCAAAGCGCCACCCACTCTTCTTATTTTTTTGTTGTTTTTTTTTTTTGTTTGTTGTATTCCAATACGGCAAAAT
CGGAGAGCGAGCGGTCGAACAAGCAAGGACTTTCTATACAGTGGGCGAATTCGAAGCTAGAAAAGCAAAGAAGAAGCCGC
ATAGCGGCAGCAGTCGTCCAGTTAAAAGCAGCATCCAGTGAGTAGCGACAATCCTACAGAGAAATCCGATCCTCCGCGGA
GGTGCCGCCAATTTTAGCACTATTCAAGTGCCGACTAAAATGGAAATTGAAACAGATCAGTCTATTGAAGCGATGGACAC
CCAGGACACCCAAGAGGTCGAGATCCTCACCAGTGACCTACAACAGACACAGCAGCAGCGAAATTCACCGCCTCAATTGC
CGAAATTCAAGAATCTTATCCAGCCACAATTGCATGCCGTTGGTGCAGTGACCCAGTTGCCCAGCGAGAACGGCAATATG
CCGCCACAACAACTATTGGCCGATAGCAGCTCCACTTCATTCGGCGATGGCGAGGCCATGGGCATTGACGATGAGTCCAA
GGAGGATCAGTTCCGTTCGGAGACGACATTCTCCTTTACCGTCGAGAATGTGGTACAATTGAAATCGCAGAGACTATCGC
CGCCAGTTTATGTCCGCATGCTGCCATGGCGGATCATGGTCATACCCAATGATCGAGCGCTCGGCTTCTTTCTGCAGTGC
AACGGGGAGAATGATTCGCCCACCTGGTCGTGCAATGCCATTGCCGAGCTGCGTTTGAAGTGCCACAAGCCGGATGCACA
GCCATTCACACGTGCCCGGATTAAGCATTTGTTCTACTCCAAGGAGAATGACTATGGCTACTCGAATTTTATCACCTGGC
AGGAGCTGAAGGATTCCGAAAAGAGCTATGTGCACAATAATAGCATTACCCTGGAGGTTCACGTCGTTGCGGATGCACCG
CATGGCGTTCTGTGGGACTCCAAGAAGCACACAGGCTATGTTGGCCTTAAAAATCAGGGCGCCACTTGCTATATGAACTC
GCTGCTGCAGACATTGTACTTCACCAATTCGCTAAGACTGTCTGTGTATCGCATACCCACGGAGGCTGACGATAGTTCCA
AATCCGTTGGATTATCGCTGCAGCGTGTGTTCCATGAATTGCAGTTTGGTGACCGTCCCGTTGGCACCAAAAAGTTAACC
AAATCCTTTGGCTGGGAGACGCTCGATTCGTTTATGCAGCACGATGTCCAGGAGTTTCTGCGCGTGCTGCTCGACAAGCT
CGAGTCGAAGATGAAGGGCACCATCCTGGAGGGCACCATACCGGGTCTGTTTGAGGGCAAAATGTCGTCGTACATTAAGT
GCAAGAACGTCGATTACAATAGCACACGCTACGAGACGTTCTACGACATTCAGCTGAACATCAAGGATAAGAAGAACATC
TACGAATCCTTTCAGGATTACGTAGCCCCCGAAACGCTGGAGGGTGACAACAAATACGATGCTGGTGTGCATGGCTTGCA
GGAAGCCAGCAAGGGCGTCATCTTCACTTCATTCCCACCCGTTTTGCATCTGCACCTGATGCGCTTCCAGTACGATCCCG
TCACGGATAGCTCGATCAAGTACAACGATCGTTTCGAGTTCTACGAACACATCAATCTGGATCGCTATCTGGCCGAGAGT
GAGAATACCTTGGCGGACTACGTTCTACACGCCGTGCTGGTGCATTCGGGCGATAACCATGGCGGGCACTATGTGGTCTT
CATTAATCCAAAGGCTGACGGACGTTGGTTTAAGTTCGACGACGATGTGGTCTCCAGTTGCCGCAAACAGGAGGCGATCG
AACAGAACTACGGCGGCATGGACGATGAGATCTCGTTTCATGCCAAGTGCAGCAATGCCTATATGCTGGTCTACATCCGA
CAGTCGGAGTTGGATCGCGTCCTTGGCGACATAACGGAAAGCGAGATATCAAGCGATCTGGTGGAGCGGCTCGATCTGGA
GAAACGAATCGAAATGGCGCGACGCAAAGAGCGCGGCGAGGCCAACACATATGTGTCCGTGCACGTAATCCTCGAGGAGA
ACTTCGAGGAACAGCACAAGCGTCGTCTCTTCGATCTGGAGAAGGTCCATCCGCGCGTCTTCCGCATCAAACAAAATCAG
ACTGTCGACGAGCTGGTGGACTTGTTTGTCAGGGGTTTCGGAGTGTCGCGCCAACGTATGCGCATGTGGAACTTGTGCAC
CGCCCAGACGCAAAAGTTTTCGCATTTCGATTTCGTGGCGGAGGGATCACGCACCATCGAGCAGATATCAACGTCACAGA
AACCGTGGGTCATCTGGCTGCAGCTGGCCTGGACGGATGTACCTGGTCCCCTGCCGCCCTTTAATCCCAAGACCGAATCC
CTGCTGTTCCTCAAATACTATGATCCGCGCAATAAGCGGCTAAATTATATTGGATGTACCCAACAGCCGCACACCCGCCG
ACTCATTGATCTGGTGCCGGATGTTAATAGCAAACTGGGCTTCGAACCGGACACCGAGCTGACCATCTACGATGAGTATG
CCGACAAGAAGCTGGTCAATCTGAATGAGCCCATCGAGAGTGCGCTGTTCATACCGCAGGATCATCTGCAAGGCCACATT
TTAATCTTCGAGCGCGAAAACGTTGACGCCAAACTCGACCTGCCCACGGTAGGGGATTACTTCCTGGATCTAGTCTACCG
CATCGAGATCATCTTCAGCGACAAATGCAATCCCAATGAACCGGACTTTACGCTGGAGCTGTCCAATCGCTATAATTACG
ATCAGCTCGCCAATGCGGTGGCCGAGCGCTTGAATACCGATCCACAGAAGCTGCAGTTCTTCATGTGCATCAACAACTAC
AAGGAGACGGCGGGCAATGCTGTACCCTACACATTTAAGGGCACCATCAAGGATCTGGTTTCTTACACCAAACAGAGCTC
AACCAAGCGTATCTTCTATCAACGACTATCGCTTAGCATCCACGAGCTGGATAATAAGAAGCAGTTCAAGTGCGTCTGGG
TGAGCAGTGATCTTAAGGACGAAAAGGAGCTGGTACTTTACCCCAATAAGAACGATACGGTGAAAGGATTGCTGGAGGAG
GCGGCAAAGAAAATCTCGTTTGCGGAGAACAGCCGTCGCAAATTGCGCCTGTTGAAGATTAGCAATCATAAGATTGTGGC
GGTATGCAAGGACGATATACCGCTGGATACGCTGCTAAAGTCCAACGAGTCAATAACGACCGCACAGGGCGCTCAAAAGA
CTTTTCGCATCGAGGAGGTGCCGGCGGAGGATATGCAACTGGCGGAGAATGAGTTCCTCATACCAGTGGCCCATTTCAGC
AAAGAGCTGTACAACTCATTCGGCATACCCTTCCTAACCAAGGCGCGCCAAGGTGAGCCATACGGTGCACTTAAGCAGCG
CATCCAGCGTCGTCTGAATGTGCAGGACAAGGAGTGGGAAAACTACAAATTCTGCGTAATCTCTATGGGTCACAATGCGG
ATGTTAATGACAATACGCCCGTTGATCTGGAGGTTTATCGCTCGTGGACCAGCGGCCAGTTGCCCTTCTTTGGCTTGGAT
CACATCAACAAGTCGCGTAAGCGCAGTTCGCTTAACTTCTCCGAGAAGGCCATCAAGATATATAACTAGGGCGGTGACAC
GAACCGGAGAAGAGGAGAAGAATTCACGCTCTAAACTGGATGATGGCGGAGCGGAGCAAGTTTGCGTGCGGAGCGGGATT
AAAAGGATGGATTGCGGGCAAGGGATTATGGCCGCACGTGTTTATTGTTTTTCAAAATTCAAAAACAAAGAGCATATTTC
TTGGAAACGATTCATACACACCACGGCACACACACACACACACACACATACACACACATGCGCGGAACTTACCCTTTTTA
CTTTTATACATATATATTGTTTGTAGTCTAACAGCTAAGTAATATTTAGGTATTTATTAGTCCCCGCCGACGACGGCAGT
GTTTGGAGATCCACTGGCCACGCCATGCCGCGCAAACTTGCTCCCTTCATTCTTCTTACTTTGTTTTACCGCTCTTCCTT
TCCTCTTACTCTCGTTTCCGTTTCCGTTTCGTCCGCCGAACAATCCTGACCAGAGTATCAGCTTTCGCATCGAGTCCAGT
ACCTCGAACTCCCGTTGACGCTGACTCATTGCTTGAACTAAAGTAACTTCAATTTAGGCTTAGGCTTCTACTACATAATA
TTCTTCTATGCTACATAATATTCTTAATCGATAATTGTTTAATCGCAGGCAGAAGTAGAAGTGAACTCGCCGCGAAGCGA
ATCGTTTCCTTTTGTCCAAGTGTAAATAAGCGTTCGTTGCTAGTTGTCCAATAAAATTATAAGCAAAAAGAAAAAAAAAA
AAAAAAAA
      Library Information (1)
      Library: LD_pOT2_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.

      (Rubin et al., 2000)
      Sample preparation

      Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

      (Rubin et al., 2000)
      Comment
      Reported As
      Symbol Synonyms
      LD
      (Rubin et al., 2000)
      LD_pOT2
      (Rubin et al., 2000)
      LD_pOT2_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)