Comment: 0-22 hr AEL
GCACTTCGACGACGGGTCACACTGGACACATGTAGCAGGAAAAAACGCAGACATCGGAGAAAGAAATCAGAAAAGTAAAT TCGCATTAAAGCCACCTCGCCAGTGCCGTGCTCGGTGGTTTTATGGAACACTCATTTCGATCACATACCGAGCGTGCGCG TCTTCCAACGACTTCCTATGACGACAAACCGAGCGGAATAAATTTAATCTGAACTCATCTTAAAGCTATGTCCGCCAAAA CTGAGGCGGACAACACGACGGCCGCCAACTCCGGCGGCGGAGGAGTCGGGAGCGGCACCTCATCCGGCGGGGGCGCATCC GCGAATGGAACAGCAACTCCGGCACGTCGTCTGCGTACCCGAAACTCAACGGGCAACGGGACTAATAGTGGAAGTGAGTC CGTAAAGAAATCCAATGCTAACGACGAGCCCTCGACCCCAGTCACCCCGGCAGGAGCAACAGGATCTCACACCCATGCTC CGCCAGGCATTTCTCCGGCGGTAATGGAACGGCCTATGCCCAGTGTTCCCATGAATCACGCCAGCAGCAGTGTGTCAGCC AGCAAAAAGTACCATAATAGTTGTCCTCATCCCACGCCCACGCCTGCGCCCACTGGGCACAAGAAGTCGGTCCACACGCA GCCGCACAGCAGCAACAAGTTTGACCAGGGAAAGAACGAGGAGTTCCACTTTGACACGCCGCCAGAGTGCCCTGTGTTCC GGCCCACAACTGAGGAGTTCAAGAATCCACTGGCCTACATCAGCAAGATCCGCTCCATAGCTGAGAAGTGTGGAATCGCA AAGATCCTGCCGCCGGCAACCTGGTCGCCGCCGTTTGCAGTGGACGTGGACAAGCTACGCTTTGTGCCACGTGTCCAGCG GCTGAATGAGCTCGAGGCAAAGACGCGCGTCAAACTCAACTTCCTTGATCAGATCGCAAAGTTCTGGGAGCTGCAAGGCT CTTCACTAAAGATCCCAATGGTGGAGCGAAAGGCTCTCGACTTGTACACACTGCACCGCATCGTTCAGGAGGAGGGGGGC ATGGAGCAAACCACAAAGGATCGCAAGTGGGCCAAGGTGGCCAACCGGATGCAGTACCCCTCAAGCAAAAGTGTTGGCGC AACCCTGAAGGCACACTACGAACGCATCTTGCACCCCTTTGAGGTATACACTTCGGGAAAGGTTCTAGGACCCACACCAA CGTCGTCTGGATCTGGTAGCACACCCGTCAAATTGGAGGACGGAGGTGGAACCGATTACAAAGCCCATGAAATTCCCACA CGGCAGCAGATTGCGCCGCCCAACGAGACAAACACACGTCGCTCCAAGCGTTTCGGAAATTCCAATGCCAGCTGCGGCCT GAGTGGTGTTACACCCACAACAAAGCCATCAGCGGGCGTATTTGTCAAGACGGAGACCAAGGAAGAATTTAAGAGGGATC TTCTCAGCAGTTTCAATGCTGTCAACAGCGGCGGATCGCCATTAGCCACAGGTACTACGGCAAACACTCGCGGAGCCAGC CAGAAGAAGGGAGGAGAGCCTCCAGCACTTATCGTAGACCCACTGATGAAGTACATTTGCCACATCTGCAATCGCGGCGA CGTCGAGGAGTCAATGCTGCTTTGCGATGGCTGCGACGACAGCTACCACACCTTTTGCCTGTTGCCGCCTCTGACCAGCA TACCCAAAGGCGAATGGCTTTGCCCGCGCTGCGTCGTCGAGGAAGTAAGCAAACCGCAGGAGGCGTTTGGTTTTGAACAG GCAGAGAGGGAATACACTCTGCAACAATTTGGACAAATGGCTGACCAGTTCAAGCAAGAATACTTCCGCAAGCCGGTGCA CTTGGTGCCCACTGAGATGGTGGAGCGCGAGTTTTGGCGCATCGTGTCATCTATCGATGAAGACGTTACCGTTGAGTATG GGGCGGACTTGCACACAATGGATCACGGCTCTGGTTTTCCCACTAAGAGCTCGTTATATTTGTTGCCGGGCGACCAAGAG TATGCAGAGTCAAGCTGGAACCTTAACAACTTGCCGTTGCTGGAGGATTCCATTCTTGGTCACATCAATGCCGACATTAG CGGCATGAACGCTCCGTGGATGTATGTGGGCATGTGCTTCGCAGCCTTCTGCTGGCACAACGAGGACCACTGGAGCTACT CCATCAACTATCTTCACTGGGGAGAACCTAAAACCTGGTACGGTGTTCCGGGGTCCTGTGCTGAGCAGTTTGAAGAGACC ATGAAGCAGGCAGCTCCAGAACTTTTTTCATCACAGCCCGATCTGCTTCACCAGCTGGTGACCATTATGAACCCGAACAT TTTGATGAACAATCGTGTACCGGTATTCCGAACCGATCAGCATGCAGGCGAGTTTGTGATCACCTTTCCCAGGGCCTATC ACGCAGGCTTTAACCAAGGCTATAACTTCGCGGAGGCCGTCAATTTTGCACCTGCCGATTGGCTGAAGATGGGGCGCGAG TGTGTTAACCACTATTCCATGCTGCGACGTTTCTGTGTCTTTTCGCACGACGAGCTCGTTTGCAAGATGGCCCTGGAGCC AGCGAAACTCACATTTGGCATCGCTACTGCCTGCTACATCGACATGGCCGAAATGGTCGACACGGAGAAGAAACTGCGCA AATCGCTTTTGGAGTGGGGCGTTACCCGCGCAGAGCGTCGCGCCTTCGAGCTAGTAAACGACGATGAGCGCCATTGCCAA GAGTGCAACACCACGTGCTTCCTGTCGGCGGTGGCCTGCGAGTGCAACGACAAGTTGATTGTCTGTTTGCGCCACTATAC GGTACTCTGCGGCTGTGCTCCTGAGAAGCACACTCTGATATATCGTTATACACTGGACGAGATGCCGCTGATGCTGCAGA AACTTAAGGTGAAGGCGCACAGCTTCGAGCGATGGCTTTCACGTTGCCGTGACATAGTCGATGCACATACACCCACCTCG GTGACCCTGCAGGAGCTGCAGGAATTGTGCAAGGAGGCCGAGACGAAAAAGTTTCCCTCCTCGCTGCTCATCGATCGCCT TAATGCTGCGGCCGTTGAGGCAGAGAAATGTGTCACAGTCATACAGCAACTGGGTATCAATAAGGTGCGAACTCGCTCGG ACCACAACCAGGAGGCTGCTCAGTACAAGCTAACCATGGAGGAGCTAGAGTTGTTCGTTCAAGAAATCGATAATCTCTGC TGCATCATTGACGAGGGCGCTTCGGTGCGAGAGCTGCTTGTTTTGGGAAAACAGTTTGTGGAGCGGTCCGAGAGCCAGCT GCAATTATCGCTGGAGTCTCTGGAGGAGAGTGAGCTGGAAACGTTAATAAACGAAGGCAGCTCCTTACGCATCGAACTGC AACAGCTCGACCTACTGCAAAAGCGACTGAAGCAGTGCAAGTGGTACAAGCGCTCGCAGGGATTGAGGGAGACCAGCTCG AAGCTGACCTACCAAGACGTGAAGAACCTGTTACACATTGCGGCTGCTGACCTTGATCCCACGGATCCGTATGTCGACAA GGAGATGCGAAAATTGCAACAGATCGGTGCAGATATCGAAGCCTGGGAGTCGCAGGCGGCCAAGTATTTCCGGCGACTGA CCCAACAGCACGAACTGGGCGAAATTGAGCAGTTCTTGAAATCGGCCTCCGACATCAACGGTCAAGTGCCTTCGCATGGA CTGCTGAAGGATGCACTACGAAAGGCGCGTGAGTGGCTACGGGCCGTTGAGCAGCTACAGCAAAACAACCACGTCACATA TTGCCACACACTGGAGGCAATGATCGAGCGCGGCTTGAACATACCCATCCAGCTGGAGGAGCTGAGCCGAATGCAGGGAC ACCTAAATAGTGCACATCAATGGAAGGACAATACAGCGTGCGCCTTCCTCAAGAAGGGCACCTTCTATACGCTCTTGGAG GTTCTCATGCCCCGGTCGGACGCCATCAACATAGACTCGGATCTCAAGCCGCGCTTCCAGGATGATTTTCTGAAAGAGAA AAATCCGGCGGAGATTGTGGCCAGCTTCAAGCACGCTGAGGAGCAGGAGCTGCTCGATATGCGCGAACTTCGGCGGCAAA ATATGAACAAGAATCCCATGCGCGATATGTTCTGCTTGTGCAAGTCCGAGTTCCGAAACTTAATGTTCAATTGTCAGTTG TGCCGCGATTGGTTTCACGAGGATTGCGTCCCACCGCCATCAGCCACAAATCAAAATGGAATTGTTAACGGCGGATCAGG ACCAGGTACCAATCGACCCAAGTGGCTATGTCCGAGCTGTGTGCGCTCGAAGAGACCGCGCCTAGAAACCATCCTTCCGC TACTTGTCCAGCTGCAGCAACTGCCCATCCGTCTGCCCGAGGACGAAGCGTTGCGTTGCCTGGCGGAGCGTGCGATGAAC TGGCAGGATCGGGCCAGGAAAGCTCTTAGCAGTCCTGACGTCAGTGCGGCTCAAGAAGCGATTATGGCGCAGCAGCAGCA GAAGCGGCGCTCCGAGGGTGGAGCAGGGGTGGGAAATATCAGCAGTCCACGGAAGCCACGTCGACGGGGGAGCCTGACGA AAGAGGCAAGTGGTTCAACAGAGTCCGATGCCGACGACGATGACGACGAGGACGAATGTCGTCTTCGAATCGTCGAAGAC AACTTTAGTAACGATGAAGATGAGCCTCGTACCGCCCCCGCGACCTCCACCGTCAACTCCGATCTCCTCAAGCTGCTGTC CGACAGTGAAATTGAAAATTTGCTTGACCTGATGATGGAGGGCGACCTGCTAGAGGTATCCTTAGATGAAACGCAGGAGC TGTGGCGCATTCTAGAAACGATGCCGCCCACGTTGCTGCAGGCGGAGGCCATGGAGCGTGTTGTCCAGTACATGCAGCGT CAGCGGCAACAGCATACAAATCCGCTGCCAACATCCGGCGCCGAAGACTCTAACGATAGTCTGATGGTACAAAATAGTCC CAATAGCAACAGCAATAGTGGCGGGGCAACGGGTTCTGCGAGCAACAGCGGACGCAACAAGAAGAGGCGCTCCAACGACA CCGGCGGCAACTCGGCAGTGCCGCGCAAGAAGCAGAGCACACCGAAGCAGACACCGGGCAAGAAGGGATCAGCGGCAGCG GCACGCAAGAGCGATGCCAAGGCATCGCCAGCAGCATCCACCACTCCGGGTGCGGATGCCGATGCCGAGAATAAGCAGGC CAACGGCGGTAATACGAACTCCAGTACAGGATCAGGTGGTGGCAATTCTGCGACGACGACGCCCACACCCGGCTCGACGC ACAAAAAGCGAAAGCGCACCTCAACCACAGCCACCAATAATAACAACAACAATAATAATAACAGCACGAACAATAGCAAC AGCAGTACCAATTTGAACAGCAACACCACCAGCGGCCAGGGAGCAGCTACTGGAGGCAATAATGCGACTGGCGGTCAGAA GAAGCACGCGCAACGGTCTCAACAGGCGGCGCAGGAGGACGACGAGGAGGAGTGCCGGGCGGAGAACTGCCACAAGCCAA CCGGGCGAGAGGTGGACTGGGTGCAGTGCGACGGCGGCTGCAACGAATGGTTCCACATGTACTGTGTGGGCCTCAATCGC AGCCAGATAAAGCCCGACGACGACTATATCTGCATTCGATGCACAAAGACAGTTGCCATCGGAACACAGGGCTCCGGTCA CAGTATGAGCGTGGCGTCCACGACGACGCCGGGCAAGCAGCGGGCGGTGCAATCGGCGCGGTAGAGTAACGTATATAGAG TCCAGGAGGTGCAGTAACAGCCGATTGTGTTTTACGCATGATGTACTACTAAATACCCGATAAAGGCCGTCTACCGGTAT TTACTAAGTTTAGTAAAGTCACTTGAGAGTTTTTGTAGTACATATTTACCAATTATCGCTATATTTACGCATTAACAACG TAAAACACATTTGAAAGAAAACGTCAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
