Comment: 0-22 hr AEL
CCGGGCTCTGTTCGAGAAATTGGGCGTTGAGTCCAACTCGAATGTTAGCTCTCGACTGTTGAGAAGTGGTTCGCGGGAGG ACAACTTATGTGACGGCTCGGATCGATCGTCCTCACGCTCCTCGGATCGCTCACAGTCGCCGCCCAAGCGGAGGACTCCG TTTCCCTCCGGGGTATCACTCGTTCACAACAACAACAATGCCGCCATTGTGGCCCAGAATGGAGTACCACCAGAGCAACG CTTGAGTAACAGCAAATTTATTGTGGAACCAGCGGCGCAAGTGGTCCCCACCTCGGTGGTCAAGTACCCGCAGCACAATA TATCCCGATTGAAGTCGGAGGAGCCATCGCCTGTTCCACCACCGGCCAGCGGGTCAGTGAGTGCTCTGTTCGCCAGTTCA GGCGGTGACAAGCCGGAGAAACCGGAGCGAAAGTTCAATTCACGCGAGCTGATCGAGAAGCAAAAGAAGTGGACCTCCCA TTTTACCAAGACCAAAACAACTCGGACGCACAGCGATCTAAACCGCTGCGATATAATACGTACAGTGCCCGGAACCGGGC TGATCATGGATAGCGAAAAGGTGGCCAAACCGGCAATGGAACCGCCACAACCTCCACCAAATGCCAGTCCCAATCCGCCA ATGCGAGCACAGGCTCCGCCAGAAATCAAGCCAAGGAGCGGGAAGATTGGCAGTCCGGTCAAGTCGCCGCCATTGCCGCC CATTCCGGCGGTCAAGCCAAAGAACGTGAGCCCGGTGAAGTTCAATCCGGACAGGTTGCGTCAGTCGCCGACTAAAACTG CGGATAATTCTCCGCCACCACCTCCGGCCAAATCGGCAGCTGTGCTTCAGAGATCCCTGATGCAGGAGCAGCAGGAGCTG TTGAGGAACTCCTGCGATCAGGGCGTAGCCCCCATTCCGCCGGAGAAGCCGCGCAAAAAGTCCGTAGATCTCATCGAGGA CACTCTGCCCTTGACCAATTGCTCCACGCCCTCATCCTGCGCCTCGCCCACCAGCTCATACTTAATGCAGCCGGCCAAGA GGGGATCGCTGGACGGCGGATCCGGAAACGGACAGTATCCCGGCAATGGACTCAGTGGCAGCACCAATTCGGCCACATCT GGCTCACCGGTGGCCAGTGCCTCCTCGGGACCTTCGTCGCCAGTTCACACTGAAGATGAAAAGCAGGAGAACGAGTCCAC GGAGAAGTCGGAGATGGAGTACTACCACGGAGGCAACTACAACAGTGTTCCCAGGCGACGACGCAGCGAAAATGAAGGTC GCAAATCTGTGGACGAATCTTCGCCATCGGCCAACAACTCCCAGCAACAGCAGCAACATTCCATTCCGGGCTCCGCCGCC GGATCTCCACAGCGAGTGGCCAACAAGCGTAGCAGCATCACGGTGAACATGCCGGCCGCCGGTCTTGGCCAGCGTCCCCC GAGCATTATATCGACCACCAGCCAGGACGAGGGTGGTTTCAATGAGTCCGCGCCCGAGCTGAAGGCCAAGCTGCAGCCCG CCTACGACCAGACGGAGGAGCAGCCGCATAGCCTGAATTACGTGGACGTCGGCTATCGCCTGAATCCCGATGGCAGCGAA AGTCGCGAGGTTTACGGATCGGAAGCTGAACTCTACGACACGGCCAAGGTGACCGATATGCAGCGCAAGTTCCACGGAGC CAATGGTTTTGGTCAGGAATCGAGCACGGTTTACGCCATTATCAAGCCTGATGTGCAGGAGTCCCAACCAGTGGCTCCCT CAAGAAGCGTTCTCATCCAGTCTCCAAATTCCTCCAGCGTGGAGGGATCTCCACTGCATCGCGGGTCGTATAGTTCCCCA CCCGTGGGTGTGGTTTCACCTATAAGGCGGCGCAATAGCAGCAATCAGGATCAGAGTGTTGGTGGTGGAGGCAGTGCCAA AACCACGCCCCAATGCTCTCCCGCCCGTTCGGCCTTAGTAAAGGGCATTGCCCCCATTGCTTCGATCGATGCGCACGAAG AGGAGGAACTAGATCTCGTAGAGGAGGACGAGCACCTGGCCGTGGAGTATGTGGAGGTGCTGGAACTCCAGCAGGACGAA GAGGAGGAAGAGGCTCCGGTCCTTCCCGAGCGACGAGCTCCGGCTCAAGGCTCCCTTGAGCTTCAGGACTTGGAGTATGC CGATACCAGCGCTGGCGAGGATGAGGAGGACATCATAAACCATCTAAAGGACGGCGATGTATTGGATGTGGAACTAATCG ACGATGTCGTGGATGAGGTGATCAAGGTTCATGTGAATCACAGTGTGGCCACTGCCCCTTCGATTCAAGCTGCTACACCA GCTGCAGCGATTCCACGGGAGGATAGCCTGCCGGATGACATGACCGCCGCCGAGGCCGAAAGATTGTTGAGCTCTAGACA ACAGTCGCTGCTGTCGGACGAACAGGCCAAGGAAGTCGAGCAAATACTCAACGCCGCCCCCAGCGTGGGCGTGGCAGTTG CTACCGTTGTTGCCACAGCAACATCGCCGACCAGCATCAAGAATCTCATTGAGGATTTGCCCGGGCAATCGGCAGTCGCC GCCAGCGCTGCCAACGGGGAGCAGGACATTCAAATCGCAGCTGTCCCAGCAATTGTCGAGGAGGACGAGGACGAAGAGGA GGACTTCCCGGAGGACGACGAGGAGGACCACGCCAGGGCCGATTTTGATGCCAACGGCGGTGACGCTGACGGCGATTCCG ATGACGTTGAAGCCGTGGACATTGTGGGCTATGGCCATGCATCCACAGCTCTGAATGCCACCTTCGTCAAGGCCGACAGC ACAGAGACAGAGACAACCACCACTACACCTTCGACAGCAACCACAGCCACCACTCGCCACGACGACGACGAGCCCGAGTG GCTAAGGGATGTCCTAGAGGCACCCAAGCGCAGCCTAGAGAACTTGCTTATCACATCCGCCACTTCGTCCAGAGCACCTG GTCAGCGTGAAGAGCTGGAAAACGGCTACGATCTACATGAGAAACATTCCGATCTCAATCAAACGTATATTACTGGTGGG GAATCACTGCACGAGTCGATAGTTTCCGTGGAGTCCACGCAATCGGATGCCACACTCAATCAGACCACGACCATCGACGA CAGTATCATCTCCAGCAAGCACAATTCCACCTACTCCCTGGCAGATGCCGAACAGGCCACCAGCTCTACGGTTTTGAGCA CTGGCGTTACCGAACTCGACGACAGTCAGTACTACATACCAGAATATCCGCCAGTTAGAAGCAAGGAGGTTCTCGTAGAG GCCGGAGTCCATTACTTCGAAGATGGCAACTTTTGGATGGAAGTGCCTGGTCTCTTAGACTTTGACGACGACGACTGCTC TTATCCGCCCATCACTGTACGCAAGAATCCCAAGGTTCGCTTTAGTTCCGGACCCATCCACGTGTACTCGACTTTTTCCG TAAACGACTACGATCGCCGGAATGAAGATGTTGATCCGGTGGCCGCTTCGGCGGAATACGAGCTCGAAAAGCGCGTGGAG AAGATGCATGTCTTCCCCGTGGAATTGATGAAGGGTCCTGAGGGTCTGGGTCTCAGTATAATTGGTATGGGCGTTGGCGC CGATGCAGGCTTAGAGAAACTAGGAATCTTTGTGAAAACCATTACCGACAACGGCGCAGCAGCCAGAGACGGTCGCATTC AGGTCAACGATCAGATTATCGAGGTGGACGGCAAGAGTCTCGTGGGCGTTACACAGGCATATGCAGCATCAGTACTGCGC AATACTTCCGGTCTAGTCAAATTCCAAATCGGACGCGAGCGCGATCCTGAAAACTCTGAGGTAGCCCAGCTCATACGACT GAGTTTGCAGGCCGATCGTGAAAAAGAGGAGCGCTTGAAGCGCCAACAAGAGGAGTACCTGCGCCGCACTCTCGACTATT CCGAGGACTCCACTCAGCCGGTTTCGGCCAACTCGAGTGTCTGCGAGGGACCCTCGAGTCCCGTCCAAGTGGAACATCCA ATGGAGGTGGAGGCCACCCACTCGCAGGAGGTAGAGTCGCTCAAGCGGCTGCTACAGGAGAGCGAAATGGGTTGCCTGGT TAAAGAGGAGATTATTCAAAATCTTAAACGAAAGCTGGTCAAGCTCGAGACGACGGGCAATGAGAACGAGCTGCTTAGCG AGCGACTGCGGCAAAGCGAGCGGGAGCTGGGCAATATCCGGAAGGAGGCGGCCAACCTGCAGAACATGCTCCAGCAGTCG CAGGGTCAGTATATGGCGCTGGACAAGAAGTACAACAAGGCCAAGCGGCTGGTCAGGGAGTACCAGCAGCGAGAGCTGGA CATGTGCCATCGCGAGGAGTTCTACCAGCAGCTGCTCCAGGAGAAGGACACCGAGTACAATGCGCTGGTGAAGAAGCTGA AGGATCGTGTGATTAATCTGGAACACGAGCTGCAGGAGACGCAGCGCAAGGCCGGTTTTCCAGTGGGCCTGCCCTACGAT AGTGCCACTCTGAAGTTGACGCCCCAGATGATGCGTAAGACGCCGCCAAAGCCGCTCTTCCACAAGCTCGAGACGGAGCT GTCGGATACGGAGATATCAGATCTCTCACCCGACGGCGATGGCGTTAAGACAGCCACTGTGGAGCGTAAGGTTCCGGTCA AGGACGAACTGGATGCGGCAGTGCCGCAGCACGAGCTGCTGGATAACTCGATTAACAAAACCAAAATCGATCTGGCCAAC CGCCAGTTGCCCTCAGCAAATGGAAACAGTAGCACATCGAATGGAGCCGCCGTTGATCTTGGCCAGCTTTCGAATGGGAA TCTGCTCAAGCGCAGCCGGAGCAACAGTCGCAGCTCGGACTGCACGCTGGATGACACGGATGAGGAGGAGGAGCGAGAGG GCTCGGCATTGAACCTAGCAGGCGCTCCTGTCGCCCATGAAACCATCAGTCTCTCCAATGGCAACTCGCATCTGCTGGCC AACGTGAATAATCTGCTGCAGCACCATCCGCCGGCAATGGCCACAGTGATCGCCACACCTTCAAATGGCCACCTGGGCAC CACCACGCCCATTCTGTTGAACTCCACCTCCTCCGCCTCGTCCAGTTCGTCCAACCAATCGACGGCACGTGAGGCACAGA TCAACCAGCTGTATGCCCAGGTGCACAAGGATCCCAGCAAGCAGCAACATCAGCAGCAGCAGCAACAACAGCAGCAGGCC CAGGCCGTGACCACCAGTATTCCTAGCATTTTCAAGAACGCTTTGGGTTCTCCGGCGGACAATGGACTCAACGACTTTCA TCGCGGCAGCATGACCACCTTTGGAACAGGTCCAGCCACAAGCAGCAATCGTGATCTCAACAGCTCGTACGACTCTATCC TGGGCTCCAACGATAAGCTAGCGGAGAACGATCCCGCCGAGAGTTGGATGTATCCCAGCAGGAGGCGAGTGGCACCGAAT GGCAGCAAAGTCCCACTGCCAGGATCCAGCTTTACTGATCAACTCAACCAGGCGCTGTCCGATCGAGAGAGGCGACTCGG CGATGGATCCTCGCGACATTCTAGCGACGATTACACGGAGATTAACAAGAGCCAGAGCGCCGCGGCCATCAACTGCAAGA CGCTGATCAACGAGATCCGCCAGGCGGTGAACGAAGCACAGCCCAAAGTGCCCTGGCAGCAGCAGCACCACCAGCAAATC CAGCAGCAGCCGTCCGCCCACACCACCGGTCCGCCGTCGCCCACCAGCATGTCCTCGGGCTGCTCCTCGCCCGGATACTC CCCCAGCCGCACCCTGGACCTGTCCGGTTCCAGCTCTAGTTTCTCCGATCGCAAGGCGATGGCAGCGGGGTATACCTACA AGGGTGGCCCTGTTCATGAATGGACCAAGGATCAGGTGGGACACTGGCTGATGGGCATCGAGCTGGAGCGTTACATCCCC GTCTTCAAGGAGAATAACGTGGAGGGCGGAGCTCTTCTTACCCTGGACTCAAAGGACTTCAAAACCTTGGGTATCTGTGG CGACGACAAGCATCGTCTGAAGAAGCGTCTTAAGGACCTGAAGGCCAACATCGAGAAGGAGCGCAAGGATATGGAGAGGG AGCGAAGGGAGCGCGAAAAGGCCATACGCAAGGCCGAGAAGAAGGCGGCCAAAAAGAAGTAGTTGAATTCTAGTTGATAA GCATCAAAGTAGCCAAAAACCAAAAGCATACGCAGTATATATTGTAGTCTATATACATACATGCATACACAACACACACA AGGAAAACACGCGCGTATATATAAACATATATCTATTCGGTTATATATTTATATGCATGTAATGATAATATAATTTATTT GTACAACATAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
