Comment: 0-22 hr AEL
ATTTAAAGAAGAGGCGGTGATTCCTGGGGAACCTGAAGTAATTAATACAAACATACTCCCGATTGGCCCGCAGGCCACCG GCAAAAAACTTCCTACCGTCAATGGTAAATCGGCGTTACTTATTAAACAGATGCCGACGGAGGTATATGATGATGAATCA GATACAGATGAATTAGATGTCAGTCCATCAACCGGAAAAGTACCTAGCTATAGCATTTATAGTGAGCAGGAAGATTACTA CATGGATCTTCAGCAAACGACACCGTCAATTCAGCCAAACGGTTTTTATGAACAAGTCAATAATGGTTATGACTACCGAG AGGATTACTTTAACGAGGAGGATGAATATAAGTATCTCGAGCAGCAGAGGGAGCAAGAGGAACATAATCAGCCGAAGAAC AAGAAATATCTGAAGCAGGCCAAAATATCAAAAATACAACCACCATCATTAGATTTCATTGACGTAGGGCAAGACGATGA CTTTATATACGACAACTATCACAGCGAAGACGATAGCGGAAATTACCTAGAGGGAAGCTCCAGTGGAAGCGTTGGTCCTA TTGAAGGGTCAATCATAAAGGTGGACTCGAATATAGAAGCTTCGTTTGCTTCGCTCAACAAGAAGAGTGACTCTTTCACA CCGACAAACGATTCCCTTCAAAAGCATGACACTGTTATTGGTGAATCAACAACCAAACTTACGCGCCTTAGGACAGAAAA AATGTGCCCAGACGTAGACGAAGAAGATGAAAACCTAAGCGATCACGTTTCCGATCTTACTGACTTAAGTAAACTAATCT CACAAAAAAAAAAAACGCTCTTGAGAGGAGAAACTGAAGAAGTCGTAGGAGGACACATGCAGGTGTTACGCCAAACGGAA ATCACAGCTCGGCAACGATGGCATTGGGCTTACAATAAAATAATCATGCAGCTTAATAATGGAGGTGGTCCTGGAGAAGT GGGATTACGTACAAATGGTCATCCCGGTGACAATCCATTCTATAGCAATATTGACAGTATGCCGGACATAAGGCCTCGAA GAAAATCCATTCCTCTTGTGTCTGAGCTGACAATGGCCGCTACAAAACGAAATGCTGGACTCACATCTGCTGTTCCACGT GCAACGCTGAATGACGAAGATCTGAAAATGCACGTGTACAAAAAAGCTCTACAAGCCCTGATATATCCTATATCCTCAAC AACGCCCCACAATTTTTTGTTATGGACTGCTACGTCACCAACGTACTGCTACGAGTGTGAGGGTCTTTTGTGGGGGATAG CACGACAAGGGGTCCGTTGCACTGAGTGCGGTGTAAAGTGTCATGAAAAATGTAAGGACCTTCTGAATGCGGACTGCCTG CAGCGTGCTGCCGAAAAAAGCTCAAAGCACGGTGCGGAAGACAAGGCTAACTCGATTATTACAGCGATGAAAGATCGTAT GAAGCAACGTGAACGAGAGAAGCCTGAAATTTTTGAGTTGATTAGGATGACATTCGGTGTGGATCCCGATACACACATAG ATTCATTAGAGCAGGCTGAGCACGCAACAGTTGAGGGAACATCTAAGTGGTCATGCAAACTAACAATTACAGTGATTTGT GCTCAAGGCCTGATAGCGAAGGATAAAAGCGGTACCAGTGATCCATATGTGACGGTGCAAGTTAGCAAGGTGAAAAAACG AACGCGAACAATGCCGCAGGAACTTAATCCTGTGTGGAACGAGAAGTTTCACTTCGAGTGCCACAACTCCTCCGACCGCA TTAAGGTTCGTGTTTGGGACGAAGACAATGACCTAAAGTCGAAACTGCGCCAGAAGTTAACTCGAGAGTCGGACGACTTT TTGGGACAAACAATTATCGAGGTTCGTACTCTTTCCGGTGAAATGGATGTATGGTACAACCTGGAAAAGCGCACAGATAA ATCGGCCGTATCAGGCGCGATACGGCTTCACATATCTGTTGAAATAAAGGGCGAGGAAAAAGTAGCTCCTTACCACGTAC AATATACCTGTTTACACGAGAACCTCTTCCATTATCTTTGCGAAGAAAATACCGGAATGGTCAAGTTGCCAACTCAAAAA GGGGACGATGCTTGGAAGTTGTATTTTGATGAAATACCCGAAGAGATTGTCGACGAGTTTTCGATGCGTTATGGAATTGA GAATATTTATCAAGCTATGACACATTTCCACTGCTTGTCTGCTAAATACCTATGTCCTGGGGTACCAGCTGTAATGAGTA CCCTTTTGGCAAACATAAATGCTTATTATGCTCATACGACTGCTTCGTCAGCTGTATCGGCCAGTGATAGATTTGCTGCC AGCAATTTTGGGAAAGAAAAATTTGTTAAGTTGCTGGACCAGTTGCATAACTCACTGAGGATCGATTTGTCAATGTACAG AAATAACTTTCCTGCATCGAGTCCAGAAAAGCTAATGGATCTAAAATCTACAGTTGATTTATTGACCAGCATAACATTTT TCCGCATGAAGGTACAAGAGCTATCTAGCCCGCCGAGAGCAAGTACAGTAGTCAAGGATTGTGTTAAAGCATGCCTTAGA TCCACTTATCAATTCTTATTTGAAAATTGTTATGAACTGTACAACAGAGAATTTCAGGTTGATCCCAACGAAGCAAAACG TGCACCAGATGATCATGAACCCAAGCTTGATAGCGTCGACTTTTGGCATAAGCTAATAGCTTTAATCGTATCGGTAATAG ATGAAGACAAAAACAGTTACGGAACCGTTTTAAATCAATTTCCGCAAGAGCTAAATATTGGACAGTTATCAGCATCTTCA ATGTGGCATCTCTTTGCTGTGGACATGAAGTACGCGCTCGAGGAGCATGAGCAACATCGTCTTTGCAAGTCATCGGCTTA TATGAATCTTCACTTTCGAGTTAAGTGGCTATATAGTAACTACGTCAAAGAGGTGCCTCCCTACAAAGGAGCGGTTCCCG ATTACCCAGCATGGTTCGAGCCTTTTGTGATGCAGTGGCTAAATGAAAATGACGACGTGTCATTGGAATACCTTCATGGC GCATTTAAGAGGGATAAAAAAGACGGGTTTCAAAAGAGCAGCGAGCACGCGCTCTTTTCAAATTCTGTAGTCGACGTTTT TACGCAATTGACGCAGTGTTTCGATGTTGTTAGCAAACTCGAGTGTCCTGATCCAGAAATTTGGAAACGTTACATGAGAC GTTTTGCTAAAACAATCGTAAAAGTGCTAATAGCCTATGCGGATATTGTGAAACTTGAGTTTCCAGAGCATATGAAGGAT GAAAGAATTGCTTGCATACTTATGAACAACATTCAGCAACTAAGGGTGCAGCTAGAAAAAATGTTTGAGTCTATGGGTGG GGATAAGCTAGAGGAGGATGCAGCCAATATTTTGAAAGAACTTCAGCAAAATCTTAACTCTGCGTTGGACGACTTAGCTT CACAATTCGCTATCAGTTTGGAGCCACGAATAACTCAATCAGTGCGAGAACTTGGAGACATGCTGCTTTCTATTAAAGGA GGCAGTGGAACCCTTGCTGCTGGTAATCTTGCAGCTCAAAGGAACGCGGTCGCCGTTGAGGCCGATGAAGTTTTAAGGCC GCTTATGGATTTACTTGATGGCTCTTTAACTCTCTATGCGCAATCATGTGAAAAAACCGTGTTGAAGAGGTTACTAAAGG AGTTATGGAAGATCGTAATGCGAATATTAGAGAAAACAATTGTTTTACCACCTATGACTGATAAAACTATGATGTTTAAA CACCTTACTGACAATGCAAAAAACTTGGCATCGAATGCAAAAATTGAAGACATGGGTCGTTTGTTCAAGTCACATATGGC AGGAAAGCAGGATGTTAAGAGTGCTCTGAGCGGGGTAATGGACATATCAAAAGAAGTAGAAAAAAACTTGTCACCCAAGC AATGCGCAGTGCTTGATGTTGCCTTAGATACAATAAAACAATACTTTCACGCGGGTGGCAATGGACTGAAAAAGACCTTT CTCGAAAAGTCGCCCGAGCTTCAGAGTTTACGTTACGCACTCAGTTTATATACACAAATGACCGACACACTAATCAAAAC CTTTATATCTAGCCAAGTTCACGAGGTCGATCTAGAAAACTCAGAGGAAGGTGTTGGGGAAATATCCGTGCAAATAGACT TATTTTCCCATCCCGGCACCGGTGAACATAAAGTAAACGTGAAAGTTGTTGCTGCTAATGACCTTAAATGGCAAATACCA TCGGGTATGTTCAGACCATTTGTTGACATTAATTTAATAGGACCGCACCTGCAAGAAAAAAAAAGGAAGTTCGCTACAAA GTCGAAGTCTAACAACTGGTCGCCTAAATACAACGAATCTTTCAGCTTCACCATTGGAAATGAGGAGCAGCTGGACTTCT TTGAATTGCACATTTGCGTTAAGGACTATTGTTTTGCTCGTGATGACCGACTTGTGGGCGTGGCTGTAATTCCATTAAAA GATATATCGGAAAAGGGATCGGTCGCATGCTGGTTACCCTTAATGAGACGAATTGAAATGGACGAAACGGGCTGGACCAT TTTAAGAATTTTGTCACAGCGAAATAATGATGAAGTGGCCAAGGAATTTGTCAAGCTTAAATCGGAAATACGGCAGGAGC CAACCATGGGTACATAAACCGGGCACATCGTACTACCAAATCCTCAAAACTTCAACAATATTAAATACAACATAACATTG TTTAATTGGACAAAACAACTTTATTTGAATTTATTCTTTGGACAGGTTTTTATTACAAGAAATCCTAGGCAACAATGCTA GTTGAAGAAATCATGCATAATATATGAATATAAATATATATCTAAACTTAAAGTATGATAAAAAAATATGTATATGTCTG GTCAGGTATACAGAAATAATACGTTTATTAGGTTAAATATTTATGTTTTAAGCAACTTTAAAATGCGCACCCACACACTT GCAAAAAAAAATTGTAATATTTCTGTCTTTTGGCACTTGTACATTTGTACATAATGCAGGGCAGTCACTGAATTAGTTAT TTTTAAATTCATTATCATCTGTACATTCTAAACTCACAACACGCACATTCCTATTTATGTAAATAAGTACCTGTATAATA ATTAATTGGTAATTCTTGAACAAATTTGTTAAAGATTCTTCACATAAGTCCATGTGAATCTTTATTTTTTTCTATTGCAA TAAAATAAAGTATATTACGTAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
