Comment: 0-22 hr AEL
AGTACATTTACATTGCAAAAAAAAGCATAAAACAAAGTTTTAAAACAATTAAAAGCGAGCAATCCTCGGCGTATCATTAA AGAACCAGCCCACTGTTCGCCCCATAACCGCAGCAGAGCGCGCGACCTTGCAAATTCACGGAGCAGCAGCGCCAGAAAGA GAGTTCGTCGGCAGAGGGGCGCAGAGAGAAAATGTCACGCAAAGCAGCAGCAGCAGTGGAAGTGGAAAAAGAGTGAAGAA ACCGCAGTAATCGCCATCGGGATCCGATCTCAGTGTCCTCCAGTGCAGCGATTCGTCACACAGACGGACCGAGGAGCACC CCAAAACGATCCTGATGCGGCACAGCCGAGTGCTGAGTGTGGCGAAACTAACCGATGGACAGCAGCAGCAGCAGCAGCAG CCAGCGAAAGTAGGAGACAACAACGGAGCCCTGCGTCTGCGGTCCTAGAAATTCGTAATTCGACGTTCACTGGCAACACG GACACCTGCTAGCGCAATGCGAATGCCGCCGATCCTGGCCAGGATTGTTCGTAGACTGGTGCTTGGATGTCGTCGGTAAT TGGGTGCGCCATCCTCCGTTGGATCTCCCCTAGCAATCCGCTGGCACCACCATGCAAAGCCTGGACTCCTCATGCCAAGA AGCCGACTTTATTATAAATGACACGCTCAAAAAGTTAAAGGGTTCCAGCAACGCGGACCATGTCCAGGGCCTAGCGGTCC ACGCCCACGGTTCGGGTGTCCACCAGACGCAGTTCGTCCAGTACGGCACGTCTGGATCCTCGCCGCAGCTGCAGCCGCAG CAGTCCTACCATCAGCTGCATCCCACGCTCCAGCAGCAGCAGTTCGCCTCCTCGAGCAGCAAATCGTACCTGGAGACCAG CCTATTGCAGGCTATACCACAGATTCCTGCCCAGCCGCCCATTTGTATCTTCGATGACGACGAGTCCCCCACCGAGGGCA CTGGCGGCTCACTGGGCAGCCTGCTCACGGAGCCGACCTCGGCCACGCCCAGCGGCCTGCCCACTGCAATTGCTCCGTCG CCCTCCTCGTGCTCATCCTCCACCACAACGATGTCGAACTTCAACTCAATCACGTCGCCGTCGCCGGTGGTGCCCGATCT TCTGCTGTCGACACCGCCGGGCGTTAACTCCAGTCTAAGCAATAGCTCAACGGGAGCGGCCAGTGTGTCCAGCAAGAAGT CCGAGAAGCGGCCACCCTCCGCCAACTCAACAGCCAGTGGAGGCGGCCACCACCTGCACCATCATCACCTCCACCAGACG CACAATCACCATGTCCTGGCATCGCCCACGTCGTCGCCCAGCAAGCGCCAGAAGTCCAATAGCAGTAGCAAGGAGAGCAG CTCCTCTAGTCGCAGCTCAGCTATACCCGCCGCATCAGTCTCCACAGCCACCTCGTCGTCGCAATCAGCGCGCGCTGGCG CCGGCGCTGCCTCCTCCAATAGCAACAGCAAGCCACCTTCATCCGTCTGCCAGTCGGGCAACAGCAAGTTCTTTAATCTG CACGAGAAAATAAGGGACCTGTACCTGCAACTGCTGAGCAACGACCTAAACTACTTTGCCGAGACAGGGCTAAAGCAGCG CACTCGATCCTTTACACTGGAGCGGTTGGTGGATCGGGAGAAGCTCAACACGATTGTGGTCAACTTGTATCCCGGCAATA AGGGATACTCGCTGGCCCTGCATTATGACGAGCACATGGTGCTAAATCCGCAAACAAACGAATGGTCCCCCACGGACAAG GACGAGACCTCGAGCGATGCTGCGGGATTGGGTACGGGAACGGGCAGTCATCGGAGTCGTCCCAAGCCGCTAGCCCAGGA CGAAAGTCAATCCAAGACGGAGGCGGGTCACGCCAAACACGATTTTGGACTCGTGGAGGTGCTTCGGTGGCCCTACGAGA ACGATTTGCTGCTGCAATGCATCGATCGTGAAATGCTGCCGGAGTTTTTAATGGATCTACTCGTAGCGGAGACCGTCAGT TTGTCGGATGGCGAGGGAACACGGGTGTACGCCAAGCCTAGCGTCTTCTATGCCGGCTGCGTTATCGCCCAGATCCGCGA CTTCCGCCAGACATTTGCCACCTCAACGAATATCTGTGATATGAAGCACATCCTGCTCAGGCCCACGAACGCCACCCTAT TCGCGGAAGTGCAGCAAATGGGCAGCCAGTTGCCGGCGGAGGACAAGCTCGCCCTGGAGAGCCAGCTGGTGTTGGCCACG GCGGAGCCGCTGTGTCTGGAGCCGGATCCCAGCATAGGGCGGCAGGCCATCAACTCCCAGCATCAGCGTCAGCTGTACAA CTCCCACGAGCTGCGGCGCCAGATGAAGAAGTTCACCCAAACGGCTATCAATCGGAAGCGCAAGTTGGACCAATTCACTC ACCATCACGGACTGGAATTGTGCGATTACCTGGCTCGCCTGCGCCAGCGACCGCGGACGGGAAGTAGCAGTGGTGGCGCC AATGCCACACCGGCCACGGCGCCCACCAACAATCCGCTGGTGGGGGCCATGCTCTCTTCGTTCACATCGAAGGTGCCCCG GCGACCACACGAGGTCATACGGCCCATTCGTCCGCCCACACTGGAGTATCCCGCCAATCTGAAGGTGCCCGAGCACGTGA TCAGCGTGGAAAAGTATGCCAAGGCCTACGAGCCCCTTAACGAATTCAGTGAGGCCTGCAAGGACGGTTGCCAGCCAAAC TGTCGTCATAACTTCCAACCGCAGCTGGTCGAGGAATACATTCTTGAGACGGAGCGGGAGGCGAGTGAAGGCCGGCGGGC TCTATACCACATAAAGCTGTCCATTTTCCAGCGCCCCTCCGATGCAGAGTATCTCGGCGAGCTCTATGTGGACCGAGATT ACCGGGAGGGTGAGCGAAACGGCGAGTCGTGCCGCTTTGCGCTGGGCACTCGGGTGCATGCCAATCGATACATTCAACAG TTCCGCGAGATCTTCACCGAGGAGGGACGTAAGGCGGTCAAAATCACGCATTTGATACCCGGGCATATGCCAATTGTGAC TCACACGGGATTGACGAATGAGCAGCGGATCCTCTTGCAGCAGCAACAACAGCAGCAGCAGCGTCAAGCTCAAATGCAGC AGCAACAACAACAGCAGCAGCAGCAAGTCGTTGTTGTTGCCAATCAGCAGCAACAATCGCAACAACAACAGCAGACACAA CAAGCGGGACAGCAGTTATCCGCCACAGTGCATCATGTTGCACTGCCGCGACAACAAATAACTCAAAAGACTCTCAATTT GGCTGGAAGTAATGTAATCAATGTACAACAAAACTTTCGGCAACAATCAGCGCAGCAGCAGCAGCCGCAAACATACACAA TTGAGGCACCGCAGCAGCAAGCTGCAGCACAGATTGTCCCGCAACAACAGCAGCAGCAGCAGCAGCAACAACAACAACAC ATACATCTTCAGCAGTTGGCCACCGGCAGCAGCAATACCTCCACAACAACGCACCAAGTTAGCCTCAGCAATGGTTCCCT GGTCCTTGTGCAACAGCAGCAGCCACAGCAGCAATCGCAACAGCTGGCACAGGCGCTGCAACATTCGGGCATAACCATCC AGCCCGCCACCATTCAACAGCAGCAACAGCAGGTGAAGGGCACTTCCGTGGTGGGGGCCAATTCAGCGCTGCGTGCCCAG CTCAACTCAAATGTGCCAATACTGCAAACGCAGCTGAAAGTGCAGCAGCAACAGATTATGCAGAAGCAACAGCAACAACA GACAACTGCCACTAGTAACAACAACAACAACAATAATAACAATAACAATATGAACAACAATACGGCCATACATCCGAATC CTGCAATAAACGCCATAGTCAACAGCATTATGAACTCTGCCAACCAGTACCAGCAACAACAGCAGCAGCAACACCAGCAG CAGCATCAGCAGCAAACGGGGAACACGTCGAGCAACAATAATGCTGCAGGGTCAGGCGGCAGCACCAACAACAACGGAAG TAGCACCAGTGCCACAACCCTCAAGAACTCCAGCAATGCGTCAATTTTAAACCTGCTCAACAGTGCTCCAGCTGCGATGA CCAGCACACCCGTGGCCAGTGGAACGTTCACCACCTCCACCGGCCAGCAGCAACCGCAGACGCTGACGCTTGTGCAGCAC CAGCAACAGTCGACGCCAAACACTGCCGATGCGGCCACCGCTACGTATGTGCAGACCACCCGTGGCACACCCACGCTGGT GAGTACGCAGCGGAAGCAGCAGTCCAGCGAAGTGTTGCAAAATCTGTTGAACGCCAATCGAAAGATTGGTGGCGGAGGCA CCACAACGACAACTTTTCGAACAAACTCCGCGGGCAATTTGATAGCGGTCAACCTCAACCAAGCCGGCCAGTCGCATCAC CAACAGACAGGCGACGGCAGCCAGACGGTTAGAGTGTCCATGTCGGCGCTTGCCTCGCAGTTGGCCTCGCCGCCCGCAGT GATGACAAACCCCACCACCAGCTACACAGTGATCTCGTCGGGCAACAGTGCCGCGGCGGCGGCAGCATGGATTCAGAGCC CAACGGGCCCAGTTCAGCAGCGCATTCTGAGCTCCCTGAGAAGAGATAGCACCACAGCGCCGCCCAACGCAATTGTGGTG GGCATGGCCGCTCCTTCTCCAGGTAGCGATTCGAACGCCTCGAATGCCAGTGGCTTTGCTGTGCCAAACAATCTAGCGTC CACTTCAAGTGGTGGTGGCGGCGGTGGTGGAGCCCTGTCCGCCTTGCTGACCAACGCAGCAACTCCCTCGCCATCGGGCT CGGATCACTCGCAGTCGTCGCAGACGCACCAAAACCAAGCCCTGCTGGAGCGCCTAAGCAATGTCACATCAAACGTATCC GCCGTTTCCATGCCGCACATGTCACCGCAACAGCCGCAGCCAACGCAGCAGTTCATCACGAAAACCATCGTTCACTCTCC CGCCACATCTTCGATACACTCGCCCATGTCTAGTCCGCATCCGCAGCCCTCTGCCTCGCCGCAGCAGCAACAACAGCAGC AGCAGCAACAGACCACCGCCACTTTGAATCTGCAGGGCATCAATCTGTCTCAACTGCAGGGCGCTATGGCTAACTTTGCC GGCCTGCAGAATGTCCAGGTGCAGATCCCCGGCTTCACACAGCCCATTTCGCTGCAGTTCTCCGGTAACAGTTTGCAGCA ACAACAGTCGGGTAATACGGCTACTGGTGCGGGCACAGCGCAGGGCCAACAGAGAAGTGTCCTGGTCTCGGTTCCAGTCT CCAGCCAACAACAGCAACTGCCACACACCATAACGCTGCAGACTCAGTCGGCGCCGCACCACCAGCAGCAGCATCAGCAG ACTCAGCAGCAACATGCCCCACCAACGGGCACCATTGTGAGCCTGCCATCGTCGGGACCTGGCACACAGACGGTCGTCAT TACGAATAACTCCGGCGGAGGAGTGACGGCGGGAAGCAGCACCAGTGGCAGTGCGGGCGGCGGTGGAGGATCAGGGGCCA CAACGGCCATGCTCACCCTTCCCATTGCTCAAATAGTCGGTGCCGGTGTACAGAAACTAAATCCTCAAACAATACGTACC TCAAATGTTGGTGGTGGTATAGGTATCGCCCAGCAAACGGTCCAGCAGCAGACGATTCAGTCGCAGTCGGGCGGCAACAG CACCGCGGCCATCCAGCTGCTGGGCACCATACAGCCGCGTGCCCGCAACGTCCAGGTGGTGGGCACCAAGCAATTGGCCA ACAGGCAGCTCATCACCACCCAGCGGCAGATAGGTGGCTCCACGCTTAAGATCGCCACCACACCCGTGAACGCTGCCAAT GTGGTGACCAGCAGCGCCAACCTAACCACCACTCCGATTGTGATGTCCGCACAGAAGCTGCAATTGAAAACGGTGAAGGC GCCCGTGCAACAGCAGCAACAGCCTCAGCAACAGCAACAGCAGCTGCAGCAACAGCACCGAATACTCAACCAGCAGAGCA CGGCCACGGTGTCGTCGCAAACACTGCCCAGTCCCAACCAAGTGCAGGTGGCCCAGCAGCAGCAGCAGCAGCAACAGCAG CTGCAGCACATTCAGATCGCCGGTAGGGCCACCACCGCTTCGGGAGCCATCAGTCAAAGGACTCTAACAGCTCTGGCGGC TGCGAAGCAGCAGCAGCAGCAACAACAGGCTGCGGTCAATCGACGGCGCTCCACCACCGATGCGACCAAGTAAGGGATGT GAATTGCCTGGAACTGAGACGACTATTGGGACGACTGAGAGATGAAGCTATAGAGCGAACAAGTAGAAGGCTACGCGTTG TGTATTGCTTGATATTATAGTTTATGGTTACCTCCTAACTATCACTACTATCGTACATTGTATACCCATATAACTATATA TGTTTTGATGTGTAATCGTAAGTGTAAATTGCATGGGCAACAGCATGGCAGCTCATAGTTGTGCAACCTAGTTAGGAGCT GGAATTGGAGCCGAAACACAATCGGTCTGGCCCGGCTCCACCGACTGCGCTTGGGGTAATTGGGTACCCCCCCCCCCCCC CCCGGCAAAAACGAATTAGGTGTAGAGGTCTTAATAATATTTATCTAGTTGTATATACGCATATATATTACATATATATT ATATATATATATGGAAATTGTTTCAATATGTCCCGCGCCCAATGGCCATTTAAACTACTACTATTTGGCTCCCGACTCCA ATTCGTTAAGTCCTAAGACTAATTCTTTAAGCGTGCTAATTTTAAGCTGTAAATCAGGGGAGGGGAGGAGTCGACGGTAA CAAAAGGAAAGCAATAATATGAGGAGAAAAATCAAGAGAAACGTAAAAATTAATTGTAAAGAAATTCTAATTATTACGAT TACGTTTAGCTGCGAGAGCATATGAACCGGGGTAGGGGTTCATTTAAGCAATTACAAATATTCATATTGTACTATTAAAA TTATTATTGCTAATTATTTAATCGATTGCTTAGCTTAAGTTCACAAACGAAGATAAAACACACACATTTTAGTTTGTAAT GTACAATTCGTAGCCTCAACCAATTTAGTACTTCCAATTGCAGATACATTAAAAACGAAGAGCAAAACAAAAAAAAAAAA AAAAAACTCGAACGACGGTCACACCCGCCTGCACACATCTGTGGAATTGTTAAATTTCGAAAGTCCGGAAAAACCGGACG GAACCTTTCACCTCGCTCTGGACCAGTGGCACCGCCCTCGAGTATCGCAAGATGTCTATTAACCTGCGCACCGTCTACGC CTTCGCCCGCGAGATGTACCCAAAGACCTCCAAGGAGACCATCCAATATGGAACAGCTGGCTTTCGCGGCAAGGCTGAGT TCCTGGACAGCGTGATGTTCCGCATGGGCGTTCTGGCCACCCTGCGTTCCCGGTATCGCGGAGGCTCCGTCATCGGTGTG ATGATCACGGCATCGCACAATCCGGAGCCGGACAACGGAGTGAAGCTGGTCGATCCCAAGGGCGAGATGCTGGAGGCCAG CTGGGAGGCGATAGCCACCGATCTGGTCAACGTCAGCGACCAGGAGCTGGAGCAGCAGGTGGCCAAGATCATAAAGGACA ACAATATCGATGTGACGACCAGTTCCCAGGTGTTTGTGGGCATGGACAACAGATACCACAGTCCGCGGCTGCTGAAAGCG GTGGCCGATGGCGTGATAGCGCTAAAGGGTAACGTCAAGGAGTACGGCATTGTGACCACACCCATGCTGCACTACTTCGT GGTGGCGGCGAATACCAAGGAGGCATACGGCAAGCCCACCGAGGAGGGTTACTACGACAAGCTGATCAAGGCATTCGAAC TGCTGCGCAACGGGCGCCTGGAGAACGGCAACTATCGCAATAGCATCATCTACGATGGTGCCAACGGTGTGGGTGCGCGC AAGATGCTGCAGTTCATCAAGCGCATGAAGGGCACCCTCAACGTGACGGTCATTAATCAGGGCATCGGTGTCGGCAAGAT CAACGAGGACTGCGGCGCCGACTACGTGAAGGTGCAGCAAAGTCCGCCGAAATCCATGCCCGAGGATGTGAAGCCGTACA CGAGATGCGTCAGCGTGGATGGCGATGCCGATCGGGTGGTGTACTTCTTTACCGACGACAAGGGTGAGTTCCATCTGCTC GATGGCGATCGCATAGCCACCCTAATCGCCGGCTATCTCATGGAACTGGTCACACAATCCGAGATTAGCCTACGGCTGGG CCTGGTGCAAACTGCCTACGCCAATGGTGCATCCACGGACTACATAGTGGACCAGCTGAAGTTTCCCGTTTCGTGCGTAC CCACGGGCGTGAAGCACCTGCACCACAAGGCCTTGGAGTACGACATTGGTGTTTACTTCGAGGCGAATGGCCACGGAACG ATTGTGTTCAGTGACAATGCTAAGGCGACGATTGCCCAGGTGGCCCAAACTAAGGAGAGTGCCAAGACGTTGCTGCTGCT CATCGATCTTATCAACGAGACCGTGGGCGATGCCATATCCGACATGCTGCTGGTGGAAACAATCCTAAATCACAAGGGCT GGGATGTCCAGGATTGGATCTCGTCGTACAACGATCTGCCCAACCGACAGCTAAAGGTGAAGGTGCAGGATCGCAATGTC ATCGAGACGACGGATGCGGAACGCGTGTGCGTAAAGCCCGAAGGTCTGCAGACGGAAATCAACCAGGTGGTGGCGAAATA CAAGCGGGGACGCTCCTTCGTTCGCCCCTCGGGCACCGAGGATGTGGTGCGCGTCTATGCCGAAGCAGCCACCAAAGAGG ACACCGATAATCTTGCCTACGAAGTGGGTCTGCTGGTCCAGAAACTGGCCGGCGGAGTTGGCCCCCCATTGACGAAGCCC AACAGCAGTGCCCACCTGTAGAGCGGAATGACGAACTAACCTCTCACGGGAATCGACAATTGACACAGATCCTGGATGGA GCAATAAATCTCTTTACTCTAAGGTGCTCAATAACTTAAAAAAAAAACAACTTAACTCTCAAACGCATTTATTCTGCAAA TGTTCACTGTCTTTTGTGTTCTAAAGATGACATATTTTTTGCCATTATTTATAAAGTTCTTTTCAAGTTTATTTATACAT ATGTACATTGTGAAAGTTGCAATTTTATGTCCTTCGCTATTTTGTAATTGAAGAATTTAATGAAAATGCAACCGCTACGT TGATTTTTTTGTTGAGCTGCTAGCGGTAACTATTGAAATTATAAAAGAATTGATAGTAGAGATTAATGAACCTAATCAAG TTTGTATTGCTATTTTCTATAAAAGTATTTTTTAAACAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
