Comment: 0-22 hr AEL
TCACACTGGTGACGTTTTAGTGAAGAAGTTGTGTTGTTTTTTTTTATTTCATTTCGTTCAATTTTCGTTGGCCATTCGTC ATCGCTTTCGTCGCAGTGTTCTTCCAAGAGTGTCGTGCACCGTAGAGGTTGACTTTTCGCCCAACGATCCAAGAAACTCA ACTTCCGGAATCCGGATGCGCGAGCGAAAACGGTAAAAGTCGTCGGTTGTCCCGTTCAACGTCCGCCTGTTGCATCTCTC CCTCTCTTTCCCGCAGGGCTGGAATTGGGGTGGAGCTCGTCCCCTCTCTTTCGCGCGCCCATTCAGCTGTTGCGTTCGCG TGCGTGTGTGTGAGTGTGCGTAATAAGATTTTCGATGTAAAGGAAAATACGCTATATTTTCCTATTTTTTGGACAAATAG GCGTGCTGCTGGGCCAAGATAAACTCCAATCAAGGAAAAAAAGGGTTTATATAACTCGCAGTGCTTTCCACCATTGTGTG TTCGCCAGCTTTTCTTCTCTTTCATTCGGCCAACTTGTTTTGCCCGCCTGCGAGTGTGTTGGTGTTTCGCATACCCTTAT GTTAAGTATTACCTAAAGATGCTGTGATGGCGAAGATGGCATCTGGAGATCTATCGCTGACTAGTACGAGTAGCCAGGAA GAAGAGAGCTCGGAGTATGTGGGCTATGATAATACCCTACGACCGCCGTCCAATTCCAGCGGCAGCACCACAGCTGCCAA CATGTCCAACAACAACGGGCCCTCGAAGGGCGTCAGTAGCGGTGTGGGCGTGTCGGGCATTTCTGGTGGAAACGGCGCAA ATTACGATATACTGCAGGCGCAGTACAAGTCCGCACTTTTGGAGCTAAATACCCTACGCCACCAGCATGCGAACACCAAG AGGCGATGCGATGAGTTAGCCACCGAGTTAACTCTGTACCAGGAGCATTACGTGGCCGATCGTAACAAGTTCACCGATAT GGTGGAGGAGAGCGCCCGTTTCAAGCGTCTGCTATTGGAGACCCAAAACCAACAGAGCCAGCAGGCCCAGAATGGAAATA GCCAAGTGCCGCCAGTCGGCAGTGGGAATCCTTATTATTTTGGCAAGACGCAGGGATGCGATGGAAGCTGCAGTGAGAAG CTGGCCGAACTGAAGAAAGAGCGAAATATGGTCGCCGTGGAGAGGGAGAAGTACAAGAAGTCCTACATCGAGCTTGAAAA GGATCGCAACTACTACCGGGAGCGAGGCGATGAAAATCAGAAACTGAAGGTTCTTCTCTCCCAGGAGAGTAAGAATGTGC TTTCCCTTACCGAGGAGCTTAATCAACTGCTCTCAGAGAAGGACAACGTGCTGCAGGAGCACCAGAAGATGTCCGACGAT CTGGTTCTGGCCAACAAAGAAATTGAGAGGCTCAAAAAGGACGAACAGCTTGCCAGGGCTGAAATCAAGGTTCTACAGCT GGCCAATGCGGACCTTAAAAAGCGCGATCTTCTAAAGTCTCGCGATAGCTCGTGGTCCAAAGAATTTCCGAGTGGAAAGG AGCTGGAGAACAGCAAGGAACTGGAAAAGCTGCGCAAGAGCTTAGAAAAGGCCCTCTCGGAGGTGGAGCGTTCAAGCCAG GATGCCGAAGAAGCGAAGCGCGTAAGGGATTGGGCCATATCGCAACGTGAGAAGATCGTGCAGGAGCGGGATTCGGTGAA GACGCTCTGTGATAAAATGCGCCACGAGAGAGATAAAGCCATCTCCGACTCGCTGATGGCCATCCGGGATAGCGAAAAGA TTAAAAAGCAGAAGGACGAAGCGCAGAAAAAGATCGACTTGTTGAAAGAACAAATGGAGCAGCAAGAGCGCCAAAATCTT GACAGTAACGCCTCCGGATCCCGACGCAGTTTCCGCCCAAGCAGTTACGAAGGTGAGGATCTCCTGGAAGTAGAGCTGTC CGGCTACGAGCACACGTCCGACCTGGGCATCATCCTGGATGACAGCAACAAGCGAAAATTAGTGTGTGGCGTTACCAGCA GTTCCCCCGCCTGCGGGAAGCTCAAGATTAACGATGTGATCTGCAAGGTGAACAACTTGGACTGCCAGTCTTTGTCGAAA CGGATGGTTCTTGATGAGATCCGCGCCTGTGCTCCTCGTTCCCTCCTGCTCGTTTCCCGAACTCGTCACAGCAAACGGCA CGCCTATTCTGTTCAGCTAAAGACTAGGGATAGGGATTGCCCGCATGGCCTGCAACTGGACATGGGAGTGTTCATTGCAA AGATCGAGCAAAATTCGCTAGCCTTCTACGAACCAGAGCTGGACGTTGGCGACCGAGTGTTAAGCATTAACAACAAGTCT ATGGACTCAGTGCAGTCAATCGAGGAAGTGATGCAGTTGATGAATGATCCCCGAAGCGATGGGCTCAATCTGTTTGCCCT TAAATACGTACAGGATCAGTTACCCCCGGGAATGACCACCTCGTCGGCGCAGACAGATTCCATTGACTCCATGCAGCATG TCTCAAGTGCCGGGGGAGGTGGAGGAAGTGGGCCCAGTAGCGCCACCAAACATCCGTCCAGGTTCGCGGAATTTTTCTTC CGAAAGCTTAAGTTCAGCAAACCAGGCACACCAGAGGATAACTTTGAGCAGGAGCACGACGACGCCATCGCTGCTTTGGA TTCTGTGCTCAGTGAAAACAGCTCTGAGAAGAGCAAGGAGAATCTGTTCAACCGCAAGAAGCGGACCAAGAAGGAGAAAG AGGCCTCCAAGAGCATGGGTACCTGGCCGCGGACCAACATCTCGCATGAAAACCCAACGGGCACCATGCGCGGCAATGAG AAGAAGCGTGCTCTGATGTCGCTTTTTACAGCCGGTCCCATAAATGTGGACAAGGATGACGAGCTGATGGGCGAGGTTGG AGTGCCAGAAAAGCAGCCAGCAGCGTTGATCCAGGATCAGTTGCCTCCACCGCTGCCCCCGCAAATGTCCAAGCCACTGG CCCACATTCGGGGATCGAACGGAGGAGCTATAAAGACGCATCCAAACCGCAATTCTAATCCGGTGAGCTCGGGAGTCTCG GCACTGTTTCCCCCCGGACCACCCAATGGAATGCCCAACACTAGTTATCCCACACATCCGAGGCATTCGCTATACGGTGT GACTGCCGAGGAGATTAACCAAAAGCCGATCCAGCGAGCCCCTCTGATGGGCGCCAATGCCAGGGTTAAAATGCCTTCAC AGGAGCGCTACGGAACCAGGCCGCATAGTAACCATCGCCTGTCGTTAAATATCACGCCCAGCGGAGATTTTTATCAGCCC AAGACCAGTGGTCAGCAGGCCCAGCAGCAAGTAATGAACGCCTCATCGACATCGGCGGGCTTTGGAGTAGGATCGGGATC AATGGTCGGTGGTACGGGTCCGGCGGCCGGAGAGTTTCCAGTGCGCAAACAGCAGGTCTATGACGTCTTCCATCCGCCTC CACTGCCCAAAAACAGCTCCGGATCCAATATGTTCATGCCGCTTCATCCGACCAGGGGCAGTCACCCGCCGCCCGTTGGA CAACCGCCGGACGTTGTGTCCCTGAAATCGCAGAATTCTATAGAGTCAATACTGTCTGCTAAGAGTCCCGCTATCAGCGA ATACGGGATGTACGCCAAGCGCCATGTGCCACAGGCGGTAAGGCATGTGCCGAAGTACCCGAGTGATAGCGAGAGCATTG GTTCCGGTGTTCACGGAGGATATGGTGGATTTCTTCAGTCCAACCATATGCCGGGAAATAGACACACGCAGCTCTTCCCC ACTTTTGGACCCGGTGGTCGGGGTAACCGGAGATCCAGTCCATTAACCCTACCTTCACCACCGCCTCAGCAGCAGTTGCA ACAGCTACCTGCGGCGACGGCACCGCACGACAGCGTTGGAATACCCACTGATCTGGACTACCATCCGCACCATCACACGC AGACCAACGCAATGCCACATCCACATCCCCATCCCCATGCTCCTTACTTGGATTACGGGCACGGTCTAGGGATAGGTCCC TATATGGGGGTGGGCGGTGGAGTCTCGGGAGTTGGCGGAGCTATCTATGAAGGCGGAACATTCCCGCGCAAGAAAGACAA TCAGCGATTAAGGATTCCATCTAATCCCAGTGTGGCTAGCAAAAGCAGCAGTATGGTGAAGAATAGCTCTGGCAGCATCG ACCATCATTATGTGACGAGCACGGGGCCAGTCTCTGGTGGATCCATGTCAGCCTCGTCTTCAGATCGTGCACCTATGTCA CTGATGAGCTCTAGTATTCACAACAGTTACGGTGCAAATATTGCAGGAGGAAATGGAACTGGATCGAGCATAGGAGTTGG AGGAGGCGGTGGAGGAGGAAGTGGCCGCGGTTCGCCCATGCCGCAGGTCCATGTGGAAGTCCTTAGCCATGGAGGCGGCG GAAGTGGCAAGCGCAACTCCAATGTGCCTGCGGATTTTCTCTGCCCCGGAGACCTTAGAAGAGTCACCATCGACAAGCGC GACAAGTCGCTAGGTATCACCATTCAGTGCAACAACAACGGCGGCGGAATATTTGTGTCAACCGTCGCTGACAAAAGCAC GGCGATGCGTGCCGGTCTCCAGGTGGGCGATCAACTCTTGGAAGTATGTGGCATTAACATGCGGGCGGCCACGCAGGAGA TTGCTGCCAATGTGCTGCGGCAGTGCGGCGACTCCTTTACAATGCTCGTTCAGTACAATCCGGAGAAGTTCCCTTCAATT GAGTACGAGGGAGCGCACAATTTGGAACCAGAATCTCCCATCAATCATTCGGGCTCCCCGACTCCGCGCAACTCTCCCCG TCCTCCTGCCCGCAACTCGCTTTTCCCGTTGCCGATGCAGCCGCAGGCTCCTTCAACGAGACCCGGCTCAAGGGCTCCGC TGTCGCATCAGTCGATTAAGGACCAGAGCTTCACCGATAGCCTTGAGAACCAGTCGGATATCAGCAGCAGCCAGGACATG CCTTCATCGGCGGCCACCACCACCACAACGACTGCCTCAGCCACGTCAACGGTGTATGATGAGGAACCTAAGCCATCCTT GCCGCCGCCACCTGCATCCGTTCCAGCAGAGACTCTAAGGTATGTGACTCTGCACATGGACAAGTCAAAGAACCTGGGCA TCAAGCTATTTGGGGGCAATAAAGTTGGCATATATGTGCATGACGTCGCGGTGGGATCGCCTTCTGATCATGCTGGAATT CGCAAAGGCGATCAAATACTGGAATACAATGGTGTAGATTTGAGCGGAGTCACCGCTGAGCAGGCGGCCAATGAGATTTC AAAGCTTACGGATACGGTCACCATGTTGGTACAGAATAAGTTACACACCCTAAAGCAAATTAAAGACGAGCCTGGCGACT CCTTTTATATTCGCGTGGGGTTTGACCGGACTGGTGAGTTGAACGAGGACGATTTGCGCTTTGTTAAGGACGAGGTGCTC TACGTGGACAATACGGTTTTTAATGGCACCTTTGGCTTGTGGCGAGCCTGGAAGCTGGATGCCATGGGCCATCGGAAGGA GTGCGGCATAATACCCAGTCAAATGAAGGTGGAGGAGGAACTGCGTTCGGGTGAGGTGGTCGACTGTGATACGGGAACGG CCAGACGCGGCAGCACTTCGGCCAGAAGATCATTTTTTCGTCGCAAGAAAAACCAGCGCAGCTCGTCCCGCGACTCCACG GAGATTGCCAGTTTCAGCAACACACAGCTTAGCTTCTTTCCAGACTTAGGTCTGCTCAACGATGATGGTGGAGCCCTTAG CTACCAGCGTGTAGAGCTTTTAGATTCACCCATTCGCCGGCCCGTGCTGATTATCGGACCGTTGTCCGAGTGTCTGATGG TTCGCCTTACAATTGACTTCTCCAACCTCTTCAAACTGTGCGAGGTGACGGCCATGGACTGTTCGCAGGAGGCCATGGAG GAGGGTCTAAAAGAAAACATCTTTGTGGATTATCGGCGGAGGGGCAACAAGTTTGAATGCACCACCGTTGAGGCGATTAG CAATGCCTGCAAAAATGATCGACGCCACTGCATCCTTGACGTTTCCATTTCCGCCGTGGAGCGTCTGCAGCGCCTACAAA TCTATCCAATTGTCCTCCTGCTGCGCTTTAAGTCTGCCAAACAAATCCGAGATATTCGCGACTTTGGCACCGACAAAATC TCAGCCAAGGCGGCCAAGGAGATGTACGAGCGGGCCATGAAGCTCGAGACCGACTATAAGCAATACATATCAGCCGTAAT CCCCGGCGTCAGTATCAAGCACATGTGCACCCAAATTAAGGATGCCGTCGACAAGGAGCAGGACAAGCTACTTTGGGTGC CCGTATCGAGTGGCTGATCCGATCGGATTGCCGCCTGGACGGACAGTTTGGACATTGGAGCATCCTAGTTTTAGTTATTA GCACACAACGCGACACTCACGATTAGTATTTTCAATGCGCATCAATAGTATCATATTTGTATTTGTTTTGTTCAACGCAG AGTATTCGATTGGCGTCAACTTGCAACTTGGAAGTTAGGGCGCGGTATTTCTACCTGTTTCTTGTCTCTGTCACCTAATT AATGTCTATAGACTTACTCTTTATTTGCGGTTAGTCCGACCGCTCTGACGGCAAAATATGGTCAGCTTTAATCACAAAAA TGGAAAATGCTTCCACAATTTATATATCTAATTTATATATACCGTTTGGAACTAGATTTCCTTCTCTGGCGACATTCTAA GCCAAAAAGTCTGTTTGTATGCAATTTATTATTATGTAATTGTAAGTAAAGGCATCTTATATTTTTTTATTATTTACCCA AACGATTCGATAAACTCGATGGCAATAGAGCGTGGCTTTAAAGCCTTCAACTTTTCAACTGTGTAAAAAAAAAAAAAAAA AAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
