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FB2026_01
,
released March 12, 2026
Comment: early pupae
TCTGAAAGTTCGGTCTCGCTCAACGATACGGATACGAAACGATATGGTACACTTGTGGAGTGGATTAACGCTAATTCGTT TTTGATTGCCGCCAGGGGGGATCGCCAATGGACCGATGCAAGGCAGCTGTTACTCCTCGCTGACGAAGGAAACCGAATAC CACCAGCTGGGATATATCCTCGCCAGTCGCAAACAAGAACAACAGGGGCACACGATCGCACCGGAGGATCACAAAGGGCT CGTGCCACATGAAGCAGGGGGTTGTTGATAGCGCCTCCTGAAAGAACTAACGACTCATGCGGTCGTGCGTCGAGACAAAG TGATCCTGCCGCAAGGAGCAGGACGAGCCGCTTATCAGCCATGCACGGGATTCGGGGTGGCTGAGGGTTGCAAACACGCG TTCTTATCTTAAAGCTCAGACTGTCGGGAGGGTGACGAAATAGAGCAGATGTGCAAACAACGGCCCCGATGACGGATGAC CGACGAAACCCAGCAATTACAGCTTGAGGGGAGGCGATAGTTACCATCCAACGACGCGTGCTCGGCACACTTGCAGATCC ACTGAATAGGAGCAACGTTCGCCTGGGCAATCAGAATCGACCTCAGACTAGGAGCTCCAAAATTCCCAGTATTATAATGT CCGTGGACAACAGCTTGGGAATTCTAGACTTATCTTCGGGTAAGGAGAGTGAGAAAACAGCAATGACAGCAGCAAACAAG GAGAACGATCCGGATTCCCATTTCACGGGTACTATCGTCACAACAGGCGCCACTGCTCAGCCAGCCACGCCCACTACCTC AAATGCCACCGCCCTACCAGCCACGCCTACTGTGGCAGCTGGCCAGGATTTGGGTGACGGTGCATGTAGTAGTAAAAATA CGGATTCCAAGTTTCAATCGTACGTAAATGGCAATGGCAATGGTGTGTACAAGATCATCAAGACACTCGGTAAGGGAAAC TTTGCCAAGGTCAAGTTGGCGATTCATGTGCCCACCGGTCGTGAAGTGGCCATCAAGGTGATCGATAAGACGCAGCTCAA TACGAGTGCACGTCAGAAGCTGTACCGCGAGGTGAAGATCATGAAACTGCTGAACCACCCGAACATCGTGCGCCTCTTCC AAGTCATCGAGTCTGAGAGGACACTCTACCTGGTGATGGAGTACGCGAGTCGTGGCGAGCTATTCGACCACCTGGTGAAG AACGGGAGGATGCGGGAGCGGGATGCCAGGGTGATCTTCCGACAGTTGGTGTCCGCCATTCAGTACTGCCACAGCAAGTT TGTGGTTCATCGGGATCTGAAGGCGGAGAACCTGTTGCTGGACCAGCACATGAACATTAAAATAGCGGACTTTGGCTTCG GCAATACCTTCGATCCCAACGCCCAGCTTGAAACGTTCTGCGGGAGTCCACCATATGCTGCCCCTGAACTTTTCATGGGC AGGAAGTACGCCGGCCCGGAGGTGGACGCCTGGTCGCTGGGAGTGGTGCTGTACACTCTCGTCAGCGGTTCGCTGCCCTT TGATGGAGGCACCCTAAAGGAGTTGCGGGAAAGAGTCCTGCGGGGCAAGTACCGTGTGCCCTACTACATCTCAATGGACT GCGAAAACCTAATGAGAAAGTTCCTGGTCCTGAATCCCGCCAAGCGCACTTCGCTTTCTGCTGTGATGTCGGATAAGTGG ATAAATCTGGGCCACGATGAATCCGACCGATTGCGTCCGTTTCGCGAGAAGCCCATGGAGCTGCAGGATGCAGCCCGGTT CGATTTACTGATGAGCATGGGCCACAAGCGCCGTGATGTGGAGCAGTCTGTAAAGGGGCAACTGTTCGACGACATCTATT GCACATACATGCTCCTGGGCGTGGCCAAGCCGCGCTCCTCGAACCGCAGTACGAAACCGGAGGCTATACCAACAGTGGAT TTAACTACTCCAGCTGTCAGTAGCCCGCTTCCTAATATCACCACACCCACTGTTACCATAGCTCATGTCACGCTTGCGCT GGACAAGAATCCGCCCATCCACTCCTCCTCAGCATCGGGTAGACCCATTGCGCCACGTTTGGCAAATGCACCCAATACAC CGACTTCAACGCCACCAACGGGACCGCCTGCCAAGCCCACAAGACGCACTCCAGCCAGGACACCAGCCCGGAAGGCCACC AACCATACGAGCGGCCAAGGACGTCCGGAGCCTTCGAGTCTTCCGCACACTCCGCAGTCAAAGAGAGCCAGTGCCAACAT GGATGTGAAGCCCACGCTGCTGAGTGCCCAGCGACAATTGGCCCAGACCCAAAAGCTGGCCAACACTCCTCCGCGCTTCC AGTACCCCGTTGCCCAAACTCCGAATCAAGCTCAGGGATCCTCCAGGCGGCCCACGACCCTGTACGAGAAAGCGGAACCT GCAGTCACTCCACTTCTGGTGCCCAAGTCGCCGGCCATGGGTGCTCGACTGCTAGAACGGATTCCCGGAGGTGGAGCAGT GGAAAAGACCTCCGACAAGCCCTTCACCCGACAAAATGTGGCACGGGCCACTTTTCATTTCGGCCAAGCGCGCAGTGGCA AACGGGGCGGTGGATCAGGAAATGGCGCAGGCGGAGAGGAGGACAGCTATCAGACGCCACCCTTATCCGCGGACGACACC AAGCCCGCCTCGCGGGTAGGTTTCTTCTCCAAGCTGACCGCACGTTTCGGTCGAAGGGTAATCCACTTGAACGAAAAGGA TGTCACCGAGCAGCGCAAGAACCTCACCAAATAGCCAGAAGTCGGGTTGTTTGGGGCAATGTCAAGGTGATAGAGATAGA ATGATAGAGTGATAAAGAAAGTGATACAGTCGGCCCTGACCGAGCATTCTAACGAAATTAAGTCAAAACTCAGCAAATCA GTTTGTCTTACAAGTGTGCGGCGAACGCTTTGCTGGGATTTAGATAAATTAATCCTACCAGAAATGACGGCAGCAAACAC AAACAAAACGACCGACAAGGAGAACGATCCCGGTCCCAATACATCGATATCCACTACCGCCACGCCCCCTTCTGCAGCTG CCCAGAATGTGGGGGGATGTTTTGGGAGCAGTGGAGGCAGATCGAGCCCTAAGTTTCAGTCCTATGTAAATGGCAACGGC TACGGAGTGTACAAGATCATCAAGACCCTTGGGAAGGGCAACTTTGCGAAAGTCAAGTTGGCGATTCATCTGCCCACCGG TCGGGAAGTGGCCATTAAGCTGATCGATAAAACTGCGCTAAATACGATTGCCCGGCAGAAGTTGTACCGAGAGGTAAATA TAATGAAAAAGCTCAACCACCCGAATATTGTGCGCCTCTTGCAAGTTATCGAGTCGGAAAGGACTCTCTACTTGGTAATG GAGTATGTCAGCGGGGGAGAACTCTTCAATTATCTGGTCAAAAACGGAAGGATGCGGGAGCGAGATGCCAGGGTGCTCTT CCGCCAGTTGGTGTCTGCTATTGAATACTGTCACAGCAAGTCCATTGTGCATCGGGATCTAAAGGCAGAGAACTTGTTGT TGGACCAGCAAATGAAATTGAAGATCGCTGATTTTGGTTTCAGCACCACGTTTGAACCAAAAGCGCCACTGGAGACGTTC TGCGGAAGTCCACCGTATGCCGCCCCAGAACTGTTCAGAGGAAAGAAGTACTCTGGACCGGAGGTGGACTCATGGTCACT GGGTGTGGTGCTGTACACCTTGGTGAGTGGTTCCTTACCCTTTGATGGAACCAATCTGAAGGAGCTCCGCGACCGTGTTC TACGTGGCAAGTATCGTGTTCCGTATTACGTCTCCATTGAATGCGAGAGTCTTATTAGGAAGTTCCTCGTTCTGAATCCA ACGCAACGTACTAGTTTATCGGCTGTCATGGCGGATCGCTGGATCAACATGGGCTACGAACAAGGTAACGGTTTGAGACC GTTTCAAGAGAAACCTATGGATCTGCATGATGTAAATCGATTGAGTTTGCTGTCGAACATGGGTCACAAACCTCGTGATG TAGAGCAGTCGCTGAAGAACCAGAAGTTCGACGACATCTATTGCACCTATATGCTCCTGGACGTTGCTAAACCGCGCTCA ACAGCCTGCAGCGAGAAATCGGGATCTTCGTTTAGGGAAACTCCTACGGCTATGCCAGGCAGCAGTCGGATTCCTGTCCC TATAGCTGCTCCCAATGTTACTATAAGCCAGGTGACCTTTGCACTGGACAAGAGTACACCGAATAGGCCTGCCGCCACTT CCATACGTCCCATGGCTCCGCGCTTGGCAAATGCCCTCACTCCGCTACCATTGACACCACCGCCCAAAAAATACATCTGC TGCTCTGCTAGCAAAGCCGCCAATCCCAGACGTTCCGAGCCGTCCAGCATTCCACAATCTGCGATGCCCAAGAAAGGAGT CGGTTCACCAGTGGATGTGAAGACAACTCTACTGAGTGCCCAACGAAAGTTGGCTGTAAACCAAAAGCTGACAAGCGCAT CACACCAAATTCGGAGTCCCATTACTCAGTCGCCAAGTCAAGCTTCAGAATGCACCAGAACACCACCGACTCATTTCGAA ATGCTGGACTCGACCAGCACTCCCCTAAAGGTTCTTAAATTGGTGGCATCCAACAGCCAGACTCCACCATCCACCGAAAA CACAAATCGTCCCACGCGAGTTGGCTTCTTCTCTAAGCTGTCCGCACGCTTTGTTAGAAGATCACTCCACAAGGGCGAAA AGGACATCAGCGAACAGGGCAGGAATCTTACCAAATAGCAAGGTGTTAGTTTAGCGCATCCCAAAAGGATTCTTTCCTGC ACAGATAATCGAAAGTGATATAGCAGCCCTCCCGAATGGACGATCGATCCACCTTATGTCTAAATGGTATTTTAATTAAA ACGACTTAAATTTAGTATAACAAATAAATGTTGATGGCATATGTAAGGTGATCGTTCTGAACTGATCTTGAAACTTGACT GTCAAAACAAATAAAATAATATTTTAACAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from larvae and early pupae.
Larvae and early pupae at various stages of development were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).