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FB2026_01
,
released March 12, 2026
Comment: early pupae
AAAAACGCGGCCAAGAAGTGTGCCAAGATGGTGCAGAAGTATTTCCAGGACAAGGCCACCGCTGCCCAGCGGGCGGAAAA GGCCCAAGAGCTGCAGCTAAAGCGTGTCGCTTCCTTTATTGCACGCGAGGTGAAGAGCTTTTGGTCGAATGTTGAGAAGC TGGTCGAGTACAAGCACCAAACTAAGATCGAGGAAAAACGCAAGCAGGCTTTAGACCAACACCTCAGCTTTATTGTAGAC CAGACAGAAAAGTTCTCACAGCAATTGGTAGAGGGAATGAACAAGAGTGTGGCGGATACGCCCAGTCTTAATTCTAGCCG TCTAACATCGCCGAAACGGGAGTCCGATGATGACTTTCGCCCTGAGTCTGGTTCAGAAGATGATGAGGAGACTATCGCCA AGGCCGAAGAAGATGCAGCCGATGTGAAAGAGGAGGTGACGGCGCTAGCTAAGGAATCTGAAATGGACTTTGATGACTTC CTTAATGATCTACCACCTGGCTATCTGGAAAATCGTGATAAGCTTATGAAAGAGGAGCAGAGCTCGGCGATAAAGACCGA AACGCCTGATGACAGCGATGATAGTGAGTTCGAGGCGAAGGAAGCCAGCGACGATGACGAAAATACCATCAGCAAGCAGG AAGAAGCCGAGCAGGAGATAGACCACAAAAAGGAGATCGATGAACTGGAGGCAGACAATGATCTCTCAGTGGAGCAGTTG TTGGCGAAATACAAGTCTGAACAACCTCCTAGTCCCAAGCGACGAAAGTTAGCGCCGCGTGATCCTGAGCTGGACTCTGA TGATGATTCGACGGCAGTTGATTCCACCGAAGAAAGCGAAGATGCGGCCACCGAGGATGAAGAAGATCTCTCTACTGTTA AAACTGATACGGATATGGAGGAACAGGATGAACAGGAGGACGGTCTTAAGAGTCTAATGGCGGACGCTGATGCAACAAGT GGTGCTGCTGGCAGCGGAAGCACGGCTGGGGCAAGCGGCAACAAGGATGATATGCTGAACGACGCTGCCGCCCTGGCCGA GAGCCTCCAGCCCAAGGGTAATACCTTGTCCTCAACCAATGTGGTTACTCCTGTGCCCTTCCTGCTAAAGCACTCCTTGC GTGAGTACCAGCACATCGGGCTCGATTGGCTGGTCACAATGAATGAGCGCAAGTTAAACGGCATCTTGGCCGACGAGATG GGTCTGGGCAAGACCATCCAGACCATTGCGCTATTGGCCCACCTTGCCTGCGCAAAGGGCAACTGGGGACCTCATCTCAT TGTGGTGCCTTCGTCTGTGATGCTCAATTGGGAAATGGAGTTCAAGAAGTGGTGCCCCGGCTTTAAAATACTCACCTACT ACGGCTCCCAGAAGGAGCGCAAGCTAAAACGCGTAGGTTGGACCAAGCCAAATGCGTTCCATGTGTGTATCACGTCCTAC AAGCTGGTGGTGCAAGATCAACAAAGCTTCCGCCGCAAAAAGTGGAAGTATCTCATCCTGGATGAAGCGCAGAACATTAA GAACTTTAAGTCCCAGCGCTGGCAGTTGCTACTTAACTTTTCCACAGAGAGGCGTCTGTTATTAACTGGAACCCCACTAC AGAACGATCTGATGGAGCTGTGGTCCCTGATGCACTTCCTTATGCCATATGTGTTCTCATCGCACCGCGAGTTTAAGGAA TGGTTCTCGAACCCAATGACTGGCATGATTGAGGGCAACATGGAGTACAACGAGACTTTAATTACTCGTCTGCACAAGGT GATTCGTCCGTTCCTACTTCGACGCCTCAAAAAGGAGGTGGAAAAACAGATGCCCAAGAAGTACGAGCATGTTATAACGT GTCGTCTGTCGAATCGCCAGCGCTATTTATATGAGGACTTCATGAGCCGCGCCAAAACTCGTGAGACTCTGCAAACGGGA AACTTGTTGAGCGTGATAAATGTACTGATGCAGTTGCGAAAAGTGTGCAATCATCCGAACATGTTTGAAGCGCGTCCTAC GATCTCGCCATTTCAAATGGACGGAATTACATTCCACACTCCGCGACTCGTCTGCGATATAATGGAATATGATCCGTTCA CGCAAATAAATCTAGAGACGTTAAACCTCTTGCTGTTGCATTTGGAGCAAACTATGACCGCCTACGTCTCGCACAAATCC CGCCTGCTCGCCCCGCCTCGCAAGCTGATCGAGGATATCGATACGGCTCCATTGCCAGCTCCCCGTTGTCCAAATGGCAA ATACCGCTTTCATATCCGAGTTCGTAGCGCTGAACTGGCGCAGCGCATCAAATTGAATGCTGTGAAGGTAGGAGCAAGTC CAGCCATGCGGTTGGAGGGTTCAAAGATTATGCCAATGCGCAATTTGCTACCAAGTGGAAGAGTGCTGAAAAGGGTCAGT GCTTCGATCAACCCTGTGAATATGGCTTTGAAACCAGTGGTGATCAATAGTGTGGTGACAACAACATCATCATCGACCAC AGCATCTTCTCCTACTGGAGCTTTAAGCGTGCTGAGCAACTCCAAGTTGCTGGGTGCACGTTCACAAATTAATGCTCCAA CGCCCGCTAAAGTAGCGAAAACGATGCAAGACGGAAAACCATTTTTCTACCTCACACCGGCGACGAATTCAGGAGCAGCA GGAGCGCGTCTTACCCTGACAAGCAAAACCACAGCCTCGGCGTCCACGACGACCTCCAGAACAACAGTTACAGCATCAAC TACTTCTGGTCAGCAACTAATAAGGGATCCCATTGTCAAAGATTTGGCCACTCATGTAAAAAGCACAGTACAAAAGCAAA GCATTGCCAATGGGAAGACGGAGCCCGAGGAAGAAACTGAAGCAGAGGATCCCTACAAAGTACAGGAGCTGATTCAGATG CGCAAGGAGCAGCGATTGGCAGCGCTTAAACGTATGGCAATGATAAATCGTCGCCGAACGGATGCCACTCCCATATACGG CGAAGATTGTCGCGAGGCTATACAGCGCTGCATGCAGGCGACCCGATCCCTAAAGCGATCAACCTGGCAGACGCGTGGAT ACGCCAACTGCTGCACTGCCATGGCGCATCGGAACGGTTGGTCCCTAAACCACTTGCTGAAGAGCTTCGAGGAAAGGTGC GCTGATCTAAAGCCAGTGTTTGCCAACTTTGTGATCTACGTTCCTTCTGTTTGTGCGCCCCGGATCCGTCGTTATGTACA AAATCTCTCATCGACGCACTGGCAGCACGAACAAAGGATTGAAAACATTGTGGATCAGGCCCTGCGGCCTAAGCTGGCGT TGCTGCATCCAATCATTTCGGAAATGACCACTAAGTTCCCAGATCCGCGTCTCATCCAATACGACTGTGGCAAGTTGCAG ACCATGGATCGTTTGCTACGCCAGCTAAAGGTTAACGGGCATCGTGTACTGATATTCACTCAGATGACCAAGATGTTGGA TGTTTTGGAAGCTTTTCTCAACTACCACGGTCATATTTATCTGCGTTTAGATGGCTCTACTCGGGTGGAACAGCGGCAGA TCCTGATGGAGCGGTTTAATGGAGATAAACGAATCTTCTGCTTCATCCTCTCCACGCGGTCTGGTGGAGTGGGCATCAAT TTGACGGGTGCCGATACTGTGATCTTTTACGACTCCGACTGGAACCCCACAATGGATGCGCAGGCCCAAGATCGTTGCCA TCGTATTGGTCAAACGCGAGATGTACATATCTACCGTCTTGTCTCCGAAAGAACCATAGAGGTTAACATTCTTAAGAAGG CAAACCAAAAGCGAATGCTGAGCGACATGGCCATCGAGGGTGGCAACTTTACAACTACGTACTTTAAGAGTTCCACCATA AAGGATCTCTTTACAATGGAGCAGAGCGAGCAGGACGAGTCGAGCCAAGAGAAGTCGGAAAACAAGGATAGAATTGTTGC TACAACAACGCTTTCAGATACGCCTTCGACGGTTGTGGAGACGGAGAAGCAGTCACTGCGTGCATTTGAGCACGCGTTGG CTGCCGCCGAGGACGAGCAGGATGTGCAGGCCACGAAAACGGCTAAAGCCGAAGTGGCAGCTGATCTGGCCGAGTTCGAC GAGAACATTCCTATTGCAACAGAAGATCCAAATGCGGAAGGAGGTCCTCAAGTGGAACTCAGCAAGGCCGATCTGGAGAT GCAGAACTTGGTTAAACAGCTCTCACCGATAGAGCGATATGCCATGCGCTTTGTGGAAGAAACTGGAGCAGCATGGACGG CGGAACAATTGCGAGCCGCGGAAGCGGAGCTGGAGGCCCAGAAACGCGAGTGGGAGGCCAATCGCTTGGCGGCCATGCAC AAGGAGGAGGAGCTGTTGAAGCAAGAAACGGAAGCGGAGGAGATGCTTACCTACAGTCGCAAGGATTCGAGTAATCAGGT CTGGATATCGCGCAACACCATGGAACAGATGCCGATGTGGTGTCCTCCCACGCCGCCGCAAGACAACGATAACGATATCT ATATTGACTATTCGCTATCGTTCATGTATGAGCTGGAACCGATTGCGGAGACGGATCTGCCGCCAGTTTACGTACGCAAG GAGCACAAGCGCTCGCGCACGGATGCTGGGTACGATGGTAGCAGACGGCCCAATAAGATGCGCCGGGAGGATAACTACGT ACCACCCAGATCGCTTTTCGATCGCCCAACGCCACAGCTGGCGCGACTGCGTCGGGAGCTGAAGAGCCAGCGATTCCGCG GAAGCTTTAAGCCTAATATGCCGATTCCAGGCCTTAAACCACAACTTCCCACCAAACCTCTTACCGAACCTGAGGCCATG GCTGAGTGGTGTGTCTTCGAGGATATGGCCATTTTGCACGTCCTGGTAAATCTGCAAGGATTACCTTGCAGCCTTATGCT GCTCTCACCGGGCCAAACGCCCAACTGGGACCTCGTCTCAGAGATGGTCAACTTCTGTTCGAAGACCTACCGTTCGGCGC GGCAGTGTCGCTGGAGGTACGAAACGCACATTCAGCCGCGTGAGGAAGGCAAGGTGGTGGAGAGTCCCAAGAAGCAGAAG AAGCTTAAGCCCACTCTGCGCACTGAGTACCTGAAGAGTCCGCTGCGATATTTGCGAACTACCCAGCTGTATGTAAGCGA TAACAACGCCTCGTTTTACAAGACGATGCGATCCCGCTTCGACAGCATTAAGACTGCTTACCTTAAGAAAGCACCGCCGC CGAAACGTCAGTTTAGTGCTCCGAGCCTGATGAATCCCAAGCACATGGAGGTGCTACAGGAGTTTGGCATCCTAAATTAC GACCAACCGGTGTCGCCGCAGAACATTGCGGCAATGAAGGCAAATAAGATTCGGGAGAAGCAGCGGGGGCAGCAGATGTC CCAGCCTCCGGTTGGCGTGGGAGTGGTTCAGCAAATGCAGCAGCAGTCTCAGCAGCAGCAGCAGCCTGCACCACCTCCGC TGCCGCAGCAACAGCAGCCGCAACAAGTGGTTCAGCAGGTTCAACAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAG CAGGTTGTGCAGCAACAACTTCCCACTGTTTCAAACGTTCAACAGACTCTGCCCGTCCAGCAAACCGTGGAGCTCGTGCA GCAGCAACCCACCACCACGACAACAGTGGCGGTGCCCGCTGCTGGTGGTCAGTTGCAGCAGCTGCAGATCCAGCACTTGA CCAGTTCCAATGTCTCGCCCGGTCAACAGACTGCCATCCTGCTCCACCAACCGCAGCAGCAATTGCGTACGCACCCCGGG CAGGGTGGACAATCCAATACTCAACAGTTGGTCAAGACTATTGTCGGCACCTCCTCCAGCTTGACCGCGGGCCAATTGCA ACAATTAGCCCAACAGTCTGCAGTCGCTTCTGGGGGCCAGTCCAGTGTGAGTGTGGTCCTAACCACTCCAGTACAGACAC TTCCGTCGGTGGTGCAACCCCAGATAGGCTCTGGTGCCCAGATCGTTTCGATCTCGTCACAGACGCTGCCCGTTAACAGC TCTCCCCAGTTGGGCAGCATTGTGCAGACCCAATCGCTGCCACAGGTGGTCTCCGTTAGCACTTTGCCCACCGTGGGCAC CGTACTGACCACCACTGCCAATCAGCCACAACAACAGCATCAGACCACGGCTGTAACCACTTTGAACACGACTATGTTGC GTGGTCAGCGGATTGTCTCGACAGCTGCAGGAAACACCCTTCAACAGCGAACGACAGCCGGTGGTCAGTCTATTGTCTCT ATGCCGAACTTAGGACAAGGCGCGAGTCCCTCGCAATTCCAGACTCAGCTTCGTTTGGCAGCTGTTCCCACATCTCCAGC CACTCAGACCACACAGTTGGTGACCACAAAGGGAATTCCGGTGAGCGCATTGCAGCAGGGAGGGAAGACGACAGTGATTC CCGTGACTCAGCAATCTGGGGGAGCACATATACAGCTCTACCGTCAGCGCAGCTTGAAGGTGCTGCAGACAACGACACAA GCCGTGCCCAGCGGATCAGCTGGCGCTACTGGAGCTACTGCTAATCTGGTGCAAGCAGGCGGCACCATAATCCAAGCTAG CAACATGGCCACGCATGTGACAAGCCAAAAAGTGGCCGTGTCGGGAATGCCGGGCACTTCCACTACTGTGCAGGCAGGCA ACGTGGTAAGCAGTGTGCAAATGCACGGACAGGCCAGGACGCAGTTCATCAAGCAGATGGCGGCCGGAAAGCAGCAGCTG CAGCGTCAAGTGGTGTCCGCTGACGGAACGACCACCACAACAGCAGCAGGGGATATGTTACTGGTTAAGCGTCATAATAT TCTTGCTGCTCAAAAGGCGCAGCAGGCCTCTGGAGCACTCTTTACGACCACCACTGGACAGCAGCAACAGCAGCAGCAGC AGCAAGGTCAACTCCCAGTGGCCGGGCAACCGCAGCAGGTAACTCAGCATCAGATCGCGTCCCTCGTTAAAGCTTCCACG GCAGCGGCGGCAAGCGGTAGCAGTGTTAATGCAGGCGGAGTCACCGTATCGGCTACTAATCCGACTGTTCAGGCGGGAAG CGTGAATATGACACTTCCACAGCTCAAGCCTGGCAGCCAGATCAAGGTAACCATGCCCAATCAGATGCGCCATCTGCAAA TGCAACAGCAGCTGACGATGCCACGAAAGATCAGCCGGATGACACAGCTGGTCAGCGCCAGTGGTCAGCCCACGGCCACC AATATAATAACGACAACTGGTCCCCAGCAACAGCAGCAGGGAGTCACAGTGTCTGGCGGCGGTACCTTGCCCACAGTTGC TTCGCAGCAGCAACAGCAGCAGCATCAGCAAAAGGTTGGAGGTGGAAACAGTGTCCAGGCACAATTGCTGCACATTCAAA ACACAAAGGGACTATCGAATTCGGTGACCATGCAGCAGATTCAACAAGTTATGCGAAGTGGCCAGCAGGGCACGTTAGCC ACCACCAATCTGGTGTTGGGTAAAACAAGCGTGGGACGGGTAATCCCCGTGTCGGTGGCTTCACAGGCCAACCAGCGTCA AACCATTCAGGTTGTTTCAGCTGCATCAGCTCAAGCCCTGGCTGCGGGAAATCTGCGCACCCATGTTGCTGGGCCGAGTA TTGCCAGTACTCTGAAGGTGGCCGCCCCTGGGAGTGCAGGAGGGCAGACCACGCAGCAGACGCTGATAGCTGCACTGCAG CATAATCAGCGGCAGAACGCCAGTCCTGTTCGGCTGCAAACGACGGCAGGAGGCAATCTTCTTGCCGTTGTGCAGCAGCA ACAGCAGCAGCAGCACACAAGTATTGCGGGTCCAACCGCTGGACCGGCAGAGGTGATGACCATCACTCAGACGACCACCA CGCTGCCCACGGTTGGCAGCTTGCAGCAGCAACAGCAGCAGCAGCAACAACAGGGCGGCATATCGCAGCCCACAACGCAA CAGCCATGAGCGATCTAACTGTTCTGGAGGATTGTGAGCTCAAGGTGAAACGCTGAAGATTACAACGATGATCGCTTCAA TCAGCTCAAGGCATGGAAGCCGTATTACTAATTTTACTGATCCTTAGATATAGCACCTTAGCTTAAGTGAAATTTCATTG TATCTATTCATATTACGAGAATAGTTTTAAGGAAGTGTAGGTGGAGTGCCTAGTGTGTAGGCTAACTGCTTTAAATTCAA TATGTAAATCGTAGTTAACTAAGTATCAGCCCCCCTATTCCCGTAGAGCAGTATGTACATTGGTAAATCTAATTCAGATC TATCAGATACTACATAAACACACCTAATATGTAGCTATAACTAAATGTCCTTTCTAGTTGTATGATCGAAAGCAAATAAA GAAAATTGTAAAAAAAAAGTTTAAAGTTTTGAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from larvae and early pupae.
Larvae and early pupae at various stages of development were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).