FB2026_02 , released June 18, 2026
Clone: Dmel\LP06502
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General Information
Symbol
Dmel\LP06502
Species
D. melanogaster
Name
LP06502
FlyBase ID
FBcl0183751
Feature type
cDNA_clone
Computed gene(s)
Dmel\Fem-1
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI17859
Library
LP_cDNA
  • LP cDNA library of D. melanogaster, iso-1 strain, larva and pupa.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    larval stage
    organism
    (Rubin et al., 2000)
    pupal stage
    organism

    Comment: early pupae

    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    2,634
    NCBI
    AY119043
    Full Length sequence dataSequence Downloader
    GTGATCAGTGATCACCGATCAGTGCAGCATCGTCCATTTCCCGGCCAGAATGGACGTCGATCCCGCAGAAGAGGCGCCTC
TAGACGAGGCACATACGTCCATATACGACGGCTATCCGCCCGATTTCGGCTACTTCAACATGGCCCACGGAATGCTGGTG
GAGATCTCCGAGGAGATCCGGGCCAAGTTTCCCCACGAGCCGCGTCTACGTTTCTCCCAGCTAAACAACCTTCACCTAGC
GGCCCGCAGTGGTCAGATGTTGAGCTTCGTCGAGATCTTGAATTCGGTTAGCTGCAGGGCGGTGCAGACCCACCTGGTGA
ATACAAATTTCGATCAGCCGGATGGACAATCGCTAACACCCCTGACCATGGCCGCGATGATGGGCAATGTGAAATTCGTG
AAGACTCTGCTGTCGCACTACGACGTCGACTTGGACCGAGAATGCAATGTCATCTACGACGGGATGGTGGTCTACGGAGC
CACAGCCCTGTGGGTGGCTGCCGGAATGGGGCATCTGCAGATAGTCAAAATGCTCGTCCAAGCGGGAGCGGCCATCAATC
ACAATACCAAGGCGCAGTCGTCGCCGCTGAGAGCGGCCTGCTATGAGGGTCGATTGGACATCGTTGAATTCCTAATTGAC
AATGGCGCTGATGTGAATGCCACGATTTGTTCAACAACAACACGCTTATGATTGCCGCCTACAAGGGTCACCACCTGGTG
GTAAAGACTCTACTTCAGAATGGATCAAGGGCCAATGATCAGGCGCTGTGCGGCGCCACTGCCTTGCACTATGCGGCCGA
AAGCGGTCACCTGGATGTGGTGGTAGCCCTGCTTGACCATGGCGCCACTCTGAAGAAGAATGAGCTGGGAATTACGCCCG
CCTTGCAGGCTGCCGAACGTCTCCATGAGGATGTCCTTGAAGCGTTCATCGAACGACCAGAACTGATGAGCTTGGAGGAG
CAGATCACAGCATTGGAGCTTCTGGGAGCCACTTATGCGAATGACAAAATCAAATACGACGTGAATAAGGCCTATGGCTA
TCTGCTGAGAGCCATGCAGCTACGTTACAGCAATCCACGCGGTGTGATCCGCAAAAGAGTTCAGCCCACTGTGCCCGCGT
ATGACAATTGGCTTGAGACTGAAAATCTGCCCGAATTGTATGCCATCAAACTTAATCACCACTCCATCCACATGGAGTCG
CTGGCCATAAGGGAACGCATTCTGGGCCGCAATAATCCGGAGTTGCCTCAGGCAATTATCTATCGAGGAGCTGTGATGGC
GGATCAGGGCAGGTTTTACCAGTGCCAGGTGCTTTGGAACTACGCCATAGATCTGCGAATGCGCAATAATGTATCGGTAG
ATCGCGATCTCTTACGGTTCGCCCAGCTTTTTGCTCAGATTTTGCGGGTGGAGAACCACAATCTTACGTTGGATCACGTT
CTGCCAGTTTTGGCCAAGTGTCAGCAGGAGATCGAGAACAATAAGTTTAAAATTAAGGAAGCCAAGCCCAACGCTTGCCC
CAGTCTATGGCAGGATCAGAATAATCAGAACTGCATTACTGCACTCTATCTAATCAAGATCGTTACCCATTTGGCGCGAC
GCAAGAAGGATCAAAACATTGACGAGGAGCACATCCAGCAGCTATTCCTGGTCGTACGCAAGTTCATACAGAACGACACT
CGCCTGCAGGATGGACAGACATTGCTGCATATCGCCGTCAATGGAGTGATGCCCGTAGACGAGTTCTACACAAATGAGAT
GTGCAGATTTCCCTGCTATGCCACTGCTTTGGTTCTGGTACACTGTGGCGCCTCCGTGGTTGCAGTGGACTCGGATCGGA
ACACCCCTCTACACATCCTAGTGACCAAGGTGAACACCACTCAGGATCGCCAGGCCGAAATGGCTCGCATTCTGGAGCTG
TTCGTCGAGGCAGGAGCCCATTTGGATGCAGTGAACGCGGCAGGGCAGACAGCGGCCACGGCCTGCAAGCTGCCTATCTT
GGCCAATCGGCTGCACGCCCATCAGAATGCCCACACCAGCCTCAAGTGCCTGGCCGCACGCTCAATTGCCACCAACAGGC
TAAACTTCAAGGGTCTGATACCCACGCAACTGGAGGCCTTCATACAGATGCACAGCGTGCGCAAGGTGCTGCCGTAGATA
GTCAATGGCTTGAGCCGCAACTGAAGTAATTAGAACTGTGAATACAATAAGCCACGTTGGAACACATTTATATATCCATA
TGTAGAAGCTTTGGTGTCTGTATGTGCGATATGTTTCGAATTTAGCGACTTAACTTAGCCAAAAGAATGCAAAGGGAATG
TTTTTTTTTAGTTTTTTACACATTTTATTTAGTATACTTCACTCTTAATTTATTTACAATTACAATCTGTAACAACTGCA
CACGTGGACTGACTGTTTGTTTGTAATCATTAATCATAATCATAACTATTAATCATACCTATGTAAAATCATTTTGTACA
TTTTTTATGTAAAACTACTATTATTCGCAGTGCAAGAGGGCGCAGGCCACCTAAGTTGAATCAATCGAATTGATTATGTT
TTTTTAGGTGCAAACGAAATAAAAAACCAAACCAATATGTAAATCACAGCTGACCAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: LP_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from larvae and early pupae.

    (Rubin et al., 2000)
    Sample preparation

    Larvae and early pupae at various stages of development were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).

    (Rubin et al., 2000)
    Comment
    Reported As
    Symbol Synonyms
    LP
    (Rubin et al., 2000)
    LP_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)