FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\LP02164
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General Information
Symbol
Dmel\LP02164
Species
D. melanogaster
Name
LP02164
FlyBase ID
FBcl0187062
Feature type
cDNA_clone
Computed gene(s)
Dmel\Ptp61F
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI19882
Library
LP_cDNA
  • LP cDNA library of D. melanogaster, iso-1 strain, larva and pupa.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    larval stage
    organism
    (Rubin et al., 2000)
    pupal stage
    organism

    Comment: early pupae

    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    2,088
    NCBI
    BT120050
    Full Length sequence dataSequence Downloader
    ATAACCAAATTAAAAGTTATTAAATCGGAACAAATCGCAGCAAAGTGTGCACAATGAGCGAGCAGAAAACGAGTGGGAGT
GGGTCGGCGGCCGCTGCCCGTCTGCAAATCGAGGCGGAGTACAAGGACAAGGGGCCGCAATGGCATCGCTTCTACAAGGA
AATTTGTGAGACCTGCGACCGGGAGGCGAAGGAGAAGCAGTTCAGCACCTCGGAATCGGAACGCCACACGAACCGCGGCC
TGAATCGCTATCGGGATGTGAATCCGTACGATCATTCCCGCATTGTTTTGAAGCGCGGCAGCGTAGACTACATCAATGCG
AACCTGGTTCAGCTGGAGCGCGCCGAGCGCCAGTACATCCTGACCCAAGGACCGCTGGTGGACACAGTGGGCCACTTCTG
GCTGATGGTATGGGAGCAGAAGTCCAGAGCAGTTCTCATGCTCAACAAGCTGATGGAGAAGAAACAGATCAAGTGCCACC
TCTACTGGCCCAACGAGATGGGAGCCGACAAGGCCCTGAAACTGCCCCACGTCAAACTCACCGTGGAGCTCGTCCGTCTC
GAGACCTACCAGAACTTTGTGCGGCGATGGTTCAAATTAACCGATCTCGAAACGCAGCAGAGTCGTGAAGTGATGCAGTT
CCACTACACCACATGGCCGGACTTTGGCATTCCCAGCTCGCCGAACGCATTCCTTAAGTTCTTGCAGCAGGTTCGCGACT
CCGGCTGCCTCAGCCGCGACGTGGGTCCAGCCGTAGTGCACTGCAGTGCCGGAATCGGGCGGTCGGGCACCTTCTGCCTG
GTGGACTGTTGCCTGGTACTTATCGACAAGTACGGAGAGTGCAATGTGTCCAAGGTGCTCTGCGAGCTGCGCAGCTACCG
CATGGGCCTCATACAGACCGCCGACCAGCTGGACTTCTCCTACCAGGCGATCATCGAGGGCATTAAAAAGCTGCACGATC
CCACCTTTCTTGACGCTGAAGAACCCCTCATTAGCAACGACACAGAGACTCACACACTGGATGAGCTGCCGCCGCCATTG
CCCCCACGCGTCCAATCGCTTAATCTGCCACTGGCCCCCAATTCCGGCGGTATACTTTCGCTCAATATGCGCGCGGCCCA
GTCTAATGGAGCTGAAAGTATCGGCAAAGAGCTGAGCAAGGACGCCCTGAACAACTTCATTAATCAGCACGACATGATCC
ACGATGCCGAGGTGGCCGACAGTCGTCCCTTGCCGCCATTACCCGTAAGGGCTTTCAACGATTCGGACAGCGATGAGGAT
TACTTGCTGGATGACGATGACGAGGACGACACCGATGAGGATGAGGAGTACGAGACCATTAACGAGCACGATGCCGATCC
AGTTAATGGACACGTGCCCGCGACAACACAGCCACATGCCGACGATGTTAATGCCAACAACGAGAAGCCAGCGGTGCCAG
TCGACGAGCAGCACAAGGCGAACGGCATCGATCCAATTCCAGGCCAGCTCCCAGCCAGTCCCGAGAACGAGCTGAAGCGG
CGAAAACGCAACGAATACCAGGCCAGCCTGGAGCAGAAGGTCAACGACATGAAGCGGAAGCAGCGGGAGAACGAGGACAA
ACAGCTGGCGGCAAAGAAACGAAGGTCATTACTCACATATATTGCCGCTGGTGTTGTGGTGGGCGTGATCTGCGCCTACG
CATACACGAAGCTAGGTTAAGTGCCCCGTAGCCACTAAGTAGTCGTAGATCAGTAGTAGCAATAGCTGTATGACGTATTT
AGTTGCCGTAGGAGACCGATTCCTCCCCTGATACCCGATTTCCGACTTCCGATCCCCACCTGCTCGTCCGCCTGACCCGG
CATCTAATGCGGGCAACGAATCAAGGACGACGTGCAACGGATCGGAAAGGATCAGAGATCGGACGGAATCGCATCGCAAC
GCCATGCAATCGCACACGAATTGCGGATGGCGGATTGATTGAGCTGAACGAACGATACAAGATGCATAAATATTAGGGAA
TTTTTTATATACGAAATACAAGAAGGAAAACAAAAGAAACAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAA
    Library Information (1)
    Library: LP_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from larvae and early pupae.

    (Rubin et al., 2000)
    Sample preparation

    Larvae and early pupae at various stages of development were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).

    (Rubin et al., 2000)
    Comment
    Reported As
    Symbol Synonyms
    LP
    (Rubin et al., 2000)
    LP_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 1 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)