FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Clone: Dmel\LP03545
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General Information
Symbol
Dmel\LP03545
Species
D. melanogaster
Name
LP03545
FlyBase ID
FBcl0187066
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG7031
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    LP_cDNA
    • LP cDNA library of D. melanogaster, iso-1 strain, larva and pupa.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      larval stage
      organism
      (Rubin et al., 2000)
      pupal stage
      organism

      Comment: early pupae

      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      1,846
      NCBI
      AY118345
      Full Length sequence dataSequence Downloader
      CAGCCAGCAGCCGCAGTAAAGGTCTTAGCCCAATCCCAGCCAGAGTTCGAAGCCCACCAGGCCCAATGGCGAACGTTAAT
TTGCAGATGCCGCTGATTGCAGCAGCCACCACCATACTGACAATTGTCAGTGGGGCCACCGCCAGCACCACATCCTCGAC
AGAGACGGCCAAGAACCAGCCATTGTATCACAATATGGGGTCTTTGTTCGAGAATCGATGGCTGCCAATGGATCAGCCGA
TGCAGCATCATCCCAATCATCGGTATGGCTCGTCCAAAATTCCACGGGCAAATGCGGGTGAAAGGAGGGCCTTTAGCAGC
GGCCATTCCTCAGGCTCCCGGCAAGGATTGGCCCATATGCTCAACGGACTAACAAATCTCTCGGGCCTGGGACACATTTC
CAATGGGTATGGCTCGGTGGCGCCCACCCATACGGAGGCACTGGCCCTGGGCTCCACCAAACAGGAGATACCGATTTACG
AGGACCACAATGAAGATGCCACCGCGGTGGCTGCCGTTGCAGCAGCTGGAGCCCATGATTTTGGCTCGGGAAATGAACAA
TCTGGCGGTGGATTGGAGAACAATCAAGAACATTCGCCGATTTACAATTATCCCGGTGCCGCACATAATGGTGGATTTTT
GCCAGCCTACGGTTCAGCTGGATCTGGTCACGAGCAGGACTACGCACAGTGGTCATCATCGGCGCCGGAGGAGCAGCAGC
CGGAACAGCAGGCGGCTCATCCCTATGCGTACGATAAAATTTCAATGGGAAATTTCCTGCCGCCGTACATGCAGGACTCT
GGATACGATGACCAGCAGGGGCAGCTTTACCACCAGCAGTTGCAACACCAACAACAGCAGCAACAGCAACAGCATCTGCA
GCAGCTGCAGCAGCAACAGCAGCAACTGTACCACCAACAGCAGCAGCAGCAGCAGCAGCGCCAGGAACAGGAACAGCACT
CTTCGTATTTCGGCCACCATGAACCATCCATTTCTGGATCCGCTGCATCATCGGCTTCATCTGCCTCGAGCGGATCTGGT
GCTGATGACTTTGAGGAGCATCCCGATGCTGGGCAGGAGCAGAGCTCCAACTACTTGTCGGAGGCAAGTGGTCATGGCCA
GGGACACACCCATCACTCGCATCATGTGGATATCATTAACTATGTGCCGGTGAAGCATGTGAAGAAGCAGCACGTGCCGG
TGGAGAAGGAGGTGAAGATACCCATATCGCATGCGGTCATCATACCGGTTCGCAAGCCAGTGCCCATTCACATACCGATC
ACCAAGAATGTTCACGTGCCGGTGGAGAAGGAATTAAAGGTTCCAGTGGAGCGACTGATTCCTGTGCCCGTGGAGAAACA
CATCCCAGTACCGGTGGAGAAGCATGTGCCCTATCATGTGGTCAAATATGTGCCCATCAAGGTGCCCAAGCCGTTTCCCG
TGAAGGTGCCCGTCTTCAAGACAGTGCTGCACAAGGTCAAGAGCTGGTGGTAGAATCTAGGATGTGCACTTTTATAAATC
CCATCCTGCATTTCTGCATTTCTGCACCTCTCATTTGCATCCAGAGCACGCGAATTTCGTTATAGTTATAAGCACATTAC
GCATACGCCACCACGTCATCCCAGCCTTTTGTGTCAGCAAACCAAAGCGAACAACATTATGCAGTTTTGCACATTTCGTA
TTCCATTTTTAATGTCGAGTTTTAAAATTCATTTAATCCCCGTGATGTACAACAATTTTGTGACTCATTTAACATTACAA
CAATTTGCATTAAAATACAAACACGAACGGGAACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAA
      Library Information (1)
      Library: LP_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from larvae and early pupae.

      (Rubin et al., 2000)
      Sample preparation

      Larvae and early pupae at various stages of development were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).

      (Rubin et al., 2000)
      Comment
      Reported As
      Symbol Synonyms
      LP
      (Rubin et al., 2000)
      LP_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)