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FB2026_03
,
released September 17, 2026
Comment: early pupae
CACCGTGCAGTTCGTGAGGAAAAGCGGAAAACCCAAGGAAACCCAGAGCGTTGCATGCCCCGCGATTCGGGGCTCGTGTC CGTGTACTGTGTACCTGTGTGCCCGCGTGCTCGTGTGTGTGTGTGCTAGCTTAGGTGGCCGAAAGATGAGATTGAAATGA AAATCCGGCAGCTCTAATCACAGCAAGCGATCGTCAGCAATAAGCGAAAGAAAAACTAAATGAAAATGCGCAATCACAAG GAGAACGGAAACGGCTCCGAAATGGGCGAGTCGACGAAGAGCTTGGCCAAAATGGAGCCGGAGAACAACAATAAGATTTC GGTGGTGTCCGTATCGAAGCTCCTGCTCAAGGATAGCAATGGGGCTAACAGCCGGAGTAGCAACAGCAATGCCAGCTTCA GTTCCGCATCGGTCGCCGGTTCTGTACAGGATGACCTGCCGCACCACAACAGCAGCAGCAGCCAGCTGGGCCAGCAACAC GGCTCATCGTTGGACCAGTGCGGATTGACTCAGGCCGGCCTCGAGGAGTACAATAATAGATCGTCCTCGTACTACGACCA GACGGCCTTCCATCACCAGAAGCAGCCATCCTATGCCCAATCCGAGGGCTACCACAGCTATGTGTCAAGTTCGGATTCCA CATCGGCCACGCCATTTCTGGATAAATTACGTCAGGAGAGCGATCTGCTGTCGCGCCAATCGCATCATTGGTCGGAGAAC GATCTGTCCTCCGTTTGCAGCAACTCTGTGGCGCCTTCGCCCATTCCGCTGTTGGCCCGTCAGTCTCACTCCCACTCTCA TTCTCACGCGCATTCCCATTCGAACTCCCATGGCCATTCCCACGGTCACGCCCACTCAGCCTCCTCATCCTCATCCAGCA ACAACAATAGCAACGGCAGCGCCACCAACAACAACAACAACAACAGCTCGGAAAGCACTTCCTCCACGGAAACCCTCAAG TGGCTGGGCTCCATGAGCGATATATCCGAAGCCAGTCATGCAACCGGCTACAGCGCCATCTCCGAATCGGTTTCCTCCTC GCAGCGCATTGTCCACAGTTCCCGGGTGCCGACACCCAAGCGTCATCATAGCGAGAGCGTGCTGTATCTGCACAACAACG AGGAGCAAGGCGACAGCTCGCCCACTGCGAGCAACTCCTCGCAGATGATGATCTCCGAGGAGGCGAATGGCGAGGAATCG CCGCCGTCGGTGCAGCCACTTCGCATCCAGCACCGTCACAGTCCCAGCTATCCGCCCGTGCACACCTCGATGGTGCTGCA CCACTTTCAGCAGCAGCAGCAGCAGCAGCAGGATTACCAGCACCCGAGTCGCCACCACACCAACCAGTCCACGTTGAGCA CACAAAGTTCCCTGCTGGAGCTGGCCTCGCCCACGGAGAAACCTCGCTCCCTCATGGGACAATCCCACTCCATGGGCGAC CTGCAGCAAAAGAATCCGCATCAGAATCCGATGTTGGGACGATCGGCTGGTCAGCAGCACAAGTCCAGCATTTCCGTGAC CATTTCCAGCAGCGAGGCCGTGGTCACCATTGCACCACAACCGCCAGCTGGTAAGCCCAGCAAGCTGCAGTTGTCCCTGG GAAAGTCGGAGGCCCTCAGTTGCAGTACACCCAATATGGGGGAGCAGAGTCCCACGAACAGCATCGATTCCTACCGCAGC AACCATCGCCTGTTCCCGGTGAGCACCTACACGGAGCCGGTGCACAGCAACACCTCGCAGTACGTGCAGCATCCCAAGCC GCAGTTCAGCTCCGGGCTGCACAAGTCCGCCAAACTTCCTGTGATAACGCCAGCGGGGGCCACAGTGCAGCCCACCTGGC ACTCGGTGGCCGAGAGGATTAACGACTTTGAGCGCAGTCAGTTGGGGGAGCCACCGAAGTTTGCCTACCTGGAGCCCACC AAGACGCACCGCCTCTCGAATCCGGCTCTAAAGGCTCTCCAGAAGAACGCAGTGCAGTCCTATGTGGAACGACAGCAGCA GCAGCAGAAGGAGGAACAGCAGCTACTACGTCCGCACTCGCAATCCTACCAAGCGTGTCATGTGGAGCGCAAATCACTGC CGAACAACTTGAGTCCCATAATGGTGGGTCTGCCCACTGGGAGTAACTCCGCATCGACTCGGGACTGCAGTTCACCCACT CCACCACCACCGCCACGACGTTCGGGGAGTCTGCTGCCCAATCTGCTAAGGCGCTCCAGTTCGGCCTCGGACTACGCGGA GTTCAGGGAGCTGCATCAGGCACAGGGTCAGGTCAAGGGACCGAGCATTAGGAACATAAGCAATGCCGAGAAAATCTCCT TCAATGACTGCGGAATGCCACCTCCGCCGCCGCCACCACGAGGACGTTTGGCCGTGCCGACCAGACGCACATCCTCGGCA ACGGAATACGCACCCATGCGGGACAAACTGCTGTTGCAGCAGGCCGCCGCCTTGGCCCACCAGCAGCACCACCCGCAGCA GCATCGCCATGCCCAACCGCCCCATGTGCCGCCCGAGCGTCCGCCCAAGCATCCCAATCTTCGGGTGCCGTCGCCTGAGC TGCCACCGCCGCCGCAGAGTGAACTTGACATCAGTTATACCTTCGATGAGCCATTGCCGCCGCCACCGCCGCCGGAAGTG CTCCAGCCACGCCCACCGCCCTCGCCCAACCGGCGGAATTGCTTCGCCGGAGCATCCACACGTCGCACCACCTACGAAGC ACCACCGCCCACCGCAATTGTCGCCGCCAAGGTGCCACCGCTGGTGCCCAAGAAGCCAACGAGCTTGCAGCACAAGCATC TCGCCAACGGAGGAGGCGGCAGTCGCAAGCGCCCGCACCACGCGACTCCACAGCCCATCCTCGAAAATGTGGCCAGTCCC GTGGCGCCACCGCCGCCCCTGTTGCCGCGTGCCAGATCCACCGCCCATGACAATGTGATTGCCAGCAATCTGGAGAGCAA CCAGCAGAAACGATCGAATTCCAAGGCCTCATACTTGCCGCGTCAGAGTCTGGAGAAGTTGAACAACACTGATCCCGACC ATGGCATATACAAGCTCACCCTGACCTCCAACGAGGACTTGGTGGCCCACACGAAGCCCAGCTATGGGGTCACAGGAAAG CTGCCCAACAATCTGCCGGATGTCCTGCCGCTGGGCGTTAAGCTCCACCAGCAGCCAAAGTTGCAGCCAGGATCGCCGAA CGGCGATGCGAATGTGACCCTGCGCTATGGCTCCAACAACAATCTGACTGGGAATTCCCCGACGGTTGCCCCGCCCCCCT ACTATGGGGGCGGCCAGCGGTATTCAACTCCTGTGCTGGGTCAAGGTTACGGCAAAAGTTCGAAGCCCGTGACCCCGCAA CAATATACGAGATCTCAGTCGTACGATGTGAAGCACACTAGTGCGGTGACTATGCCGACAATGTCCCAGTCCCACGTGGA TCTCAAGCAGGCCGCCCATGACCTAGAGACGACGCTGGAGGAGGTGCTGCCCACTGCCACGCCCACGCCGACGCCAACAC CGACGCCCACACCGCCACGCCTCTCGCCGGCTTCCTCGCACTCGGACTGCAGTCTGAGCACCAGTTCCTTGGAGTGCACA ATCAATCCTATAGCGACACCGATTCCTAAGCCTGAGGCGCACATCTTTCGCGCCGAGGTGATTAGCACCACCCTGAACAC AAATCCGTTGACAACACCGCCCAAGCCCGCGATGAACCGCCAGGAATCCCTGAGGGAGAACATCGAAAAGATCACCCAAC TACAGTCGGTGCTGATGTCGGCGCACCTGTGTGATGCGAGTCTACTAGGTGGTTACACCACTCCACTGATAACCAGTCCC ACTGCCAGTTTCGCTAACGAACCACTAATGACACCACCACTGCCGCCCAGTCCGCCACCGCCACTAGAACCGGAGGAGGA GGAGGAGCAGGAGGAGAACGATGTGCACGACAAGCAGCCAGAGATCGAGGAACTGCAGCTGATGCAGCGCAGCGAATTGG TCCTAATGGTGAATCCCAAGCCGAGCACAACGGATATGGCCTGCCAAACGGACGAGCTGGAGGACAGGGACACGGACCTC GAAGCGGCACGCGAGGAGCACCAGACTAGAACGACTCTGCAGCCGCGACAGCGCCAGCCCATCGAGCTGGACTACGAGCA GATGAGCCGGGAGCTGGTTAAGCTCCTACCGCCTGGTGACAAGATCGCCGACATCCTCACACCAAAGATCTGCAAGCCCA CCTCGCAATACGTTAGCAATCTGTACAATCCGGATGTGCCACTGCGCTTGGCCAAGCGCGATGTTGGCACCTCTACGTTG ATGCGAATGAAGTCCATCACGTCGTCTGCCGAGATCCGAGTGGTCAGTGTGGAGCTGCAGCTGGCAGAGCCGAGCGAGGA GCCGACGAATTTAATCAAGCAAAAGATGGATGAGCTCATCAAGCATTTGAACCAAAAAATTGTCTCCCTGAAACGCGAGC AGCAGACGATCAGCGAGGAGTGCTCGGCCAATGACAGACTGGGCCAGGATCTATTCGCCAAGCTAGCGGAGAAGGTTCGA CCCAGCGAAGCCTCCAAGTTCCGTACCCATGTCGACGCCGTGGGCAACATAACCAGTTTACTTCTGTCGCTTTCCGAGCG TTTGGCCCAAACCGAAAGCAGCCTGGAAACGCGCCAGCAGGAAAGGGGCGCGCTGGAATCAAAGCGGGATCTGCTGTACG AGCAGATGGAGGAGGCGCAGCGTCTCAAATCGGACATAGAACGACGTGGAGTCAGCATCGCCGGATTACTGGCCAAGAAC CTCAGCGCGGACATGTGCGCCGACTACGACTACTTCATCAACATGAAGGCCAAGCTGATCGCCGATGCACGCGACCTGGC CGTAAGGATCAAGGGCAGCGAGGAGCAGCTTAGCTCCCTCAGCGATGCGCTAGTCCAAAGCGATTGTTAGCCTGGCCAAC AGCTAATGGGCAACTGGGCCGATTCGATTCTCCCTGCTGTCAACTCAAAGTTGAGAGCCGATCGAGAGAGCCCGCAGGAT CGGCCAGTTTAGTCCGCAATGTGTATTAGTTTAAAACGATGTGCTTAATTACCACACCCACACTATAATGTATAAATATT GTATGTTCATTTGTGATTTTCAACTAGCTAGTAGGTTAATTATTCTATATATCGGCCGCATAGGTGTAAGCATTTTCTAC ATAATTATCAAAGCAAAGATATACCGTATGCATTAATAAAAGTATGAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from larvae and early pupae.
Larvae and early pupae at various stages of development were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).