FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\LP06602
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General Information
Symbol
Dmel\LP06602
Species
D. melanogaster
Name
LP06602
FlyBase ID
FBcl0191257
Feature type
cDNA_clone
Computed gene(s)
Dmel\dpr15
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI05813
Library
LP_cDNA
  • LP cDNA library of D. melanogaster, iso-1 strain, larva and pupa.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    larval stage
    organism
    (Rubin et al., 2000)
    pupal stage
    organism

    Comment: early pupae

    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    3,508
    NCBI
    AY122210
    Full Length sequence dataSequence Downloader
    TCGCTTCTCGCTTCACAGCCGCCGAACGCAGATTAAACATCCTCCACGGAGAGCCCCAAATGCTTTTAGTTATTTTAGAT
TTCCCCGACAAGCTTACCGTGCAAGTGCAATTTGAAAATATGTTAAACCTGCGATTTTGTGCGCCGAGAAAACGCCAGCA
ACGCGAAGCACAAGAAGATATTGCTGCAGTTCGTGTCAAGGCGTATACGCGATATTAACAAGAGCAACAGCAGCAGCATC
CAACAAGAGCGGATTTGGACTCGGAATCCCCGCCGAGGCAGGGGATAAATTTACACAGGCTCCAGCTCCAGCTATACCTT
CAGCGGCCCAACGCCGCCAGACAGCCAATGTGACCCAGTTAAGGCCACAGGATTTAGCTGGCCAAGGACCGCGAACCAAG
GAACCGAATCGGAAACCCGGAACGAGATACGGGATACGCGGAGCTCGGGGCGCGAAGCACGTCCAATACCAGACTCGACA
CGGAGCAAGGATTATCAATTTGCTTGGCACTTTAATGGAGTTGGCCAAGATGCCGCGGCGCCTGATCTTGCTGGCCCTGC
TCATCTGTTTGCCGGCCATCAGAGGGCAGGCGGAGGAGACATCAATATTAAACAGCAATGGAACAAATGGGCGAGCATTT
CAAAGGACTCAATCAAGGCGCGAGGCATTAATTCCGGCGACAACGCAAATGGCCCAGACGCTGGACAAAGCCAGCGCATT
TAGGCCAACAATGGCTGCAGCTCTGGATGCAGCGGCTCCGAATCGCTTCAGCAACAATGTAGCCATTAATAACATTAGCA
ACAATGGCGATCAGCGACTGAGGCTGACCGCTGCCCTCGAGGCGAATCTGATTATGAGCAATCGGAATGTCCAGGAGCAG
CACAGCTATAGAATCACGCAGGACAACGCCACATCTGCAGCTTCGATTGCCCCGAATGGAACGGCATTTGGGGATGCCCC
CTCAATCCCAATAGGAGTAGCTCCAGCGACAACAACAACAATTGATCCTGCCACAACGACGCCAACAACAACAACGAGGC
GTACAACACGCCGTCCGGTGGCAACAACAACGCTGAAGCCACCGCCAACAATAGATGATTACCAGACAATAATTAGCCAG
GCGGGCACCCATGCCTATCTGCCGTGCAATGTCAAGCAGCTGGTGAAGAAGCCGATATCCTGGCTGCGGATGAGGGACGG
GCACATCCTTACCGTAGACCAGACCACCTTCATCGCGGATCAGCGCTTTCAGTCCGTGTTCTCGCCGAATCCTGAACGTT
GGTCCCTCCAGATCAAGTACGTGCAGCTGAAGGACGAAGGAACCTATGAGTGTCAGGTGTCCACGGAGCCCAAGGCCAGT
GCCATTGTCCATCTAAGGATTGTGGAGCCGAAAACCGAATTAATTGGCGAGTCAACGCGACATGTGAAGGCCGGAAGTCA
AGTGAAATTGCGTTGCATAATTAGCCAGGCTCTGGAGCCGCCGCTTTTCATTAATTGGTTTTACAATCAGAAGCAAATCT
ATTTGCATAATCGTCGCGGTTGGCGTACGGAAATTGAACGAATCGATCTGCCAGCGGAAGTGCCAACCACCAGCACCACC
ACCACAACGACAACAACAACCGCCAGCACCACGACCACGACCACCAGCACAACGCCAGCGACACCATCGACAACGGCCAC
AGGATCAACAGAAGGCGCCACATCCTCGGAGACGCTTAATGGCCTGGTAACCATAACACGTTCATATATTCTAGATGCCA
TATCCCAGAATGATGTTTCCGAACTGGGAGCCGCTGCTGGAGTGGCTGTTGCAACGGAGACGTCAACAGCTCAGCTGCTT
ACCGAAGTGGAGGCAACGTCTTCGACTTCTGGGACGTCGACAGGAGCAGGACTCCTTGCCTCCACCAGTGCTGCATACGC
AGCAGGAGCTGCAGCAGGCATAACTACTGCTGCGACAGGTGACTCCGCGGCAACAGCAGCAACAACCAGCGCCTGGCTGA
CAACAATGGATGCTGAGGCGGCAACAACTGCGGCCACAACAACGACAACCATGCTGCCAAGCAGCAGTTTCATTAAGCAG
ATAACGACGGCTTCTCTCATCATTCCGGCCGTAGTCAAACTGGATTCCGGCAACTACACGTGCAGCCCCTCGAACAGTGC
GCCCCGTACCATTGTCCTCCACGTGCTGAACGGTGAATATTCCGCCTCGGCCATTAAGTCGGGCAGTGTCAGCTGGAGTG
CGCTAATTGGATGTCACGGCTATTTGCACTGGCGGAATGTTTCAACGCTTCTGACACTTTTGTGGATTATTAAATTTGCA
CTGGCCAGGGACATCTGCCAGCCGAATGCCACCAGCAAAGCCACCACAAGGACATCGCTTCTACGCGCAGGCGATGGAGC
AACAGCAGATGTGACAAGGGAAACAGGACCCAAATCAGGGGCCAGTTTCCACAGGGTCAGCAGCTCAATTAGTGCCACCA
AAGTGGCCGAGGACAAGACTTGAGTTGTCCTCAGGCAATCGCCTAGTTACTTGGATCTTACGCATTAAAGGAGGCTACGA
CTTAAGATTTTACTCGATTTAATTACATATTAACAGAGAGAGAATAAGGAAGATACACCCTCGGCAAGGCAATATATACA
GGACTAAGGGCATAAGTTAAAGTTATAATCATACTCGATTTATGTTTGTTTCCCATCTGAGTGTAAATTGATATTGAGAT
TCATGCTTATGTTCCGCCATATCATATAGTTTCATTGAAAAGTTGCAAGAATTGTGTAAATAGAATGCACAGAATTCGCC
CTTAACAGATAAGAACATTTCCATTATTTTAATCAATTGGAATCTGCCATGGTTGTATCATATATAAGTTAAATAATCAT
AAATGATTCATAAGTCTTGAGAGCACTAACAAATTTTCAATAGGATTTTTATGAAGTAAAATCAAATGTGTAGTTCAGCT
CGTGTAGCACGATTGAATAAGTATCGAATGAATTTCAAATTTCTTTGGTTTCCTTAAGCTATAAATTGTAAATATGTATG
TATACCTTATGATATCTGTATGAATACCCTGATTTTTTAAGTCTTAAAATCCATTTTTATGCATAAAGGATAATGATAAA
CGAATACTGAATACAAGTGTAAATAAAAGGGTGTTACTGCGTTCTATTCAAATAAATATCCCAAGCAAGCCAAAAAATTT
AATGTAAACATTAGTTAGTATATAAAAGACTTACTTAAATTTTAGACGCAGCCAAGCCACAAAAACCCAATGTGAAACCA
AAACTTAATCAACTAAATAAACAGAATTGTAAGGAGTTGCTCCAACGTTGCGTATACTTGATTTGCAACACGACATTTTA
AGAAAATGCAAATGAAAATTAAAATTAAATGAAAATCAATTGAAACGAAATGAAGTGTTTGCAATAATTCATAAGCCATA
AGATCTAAGTGAAATACAAGTAAATAAAATAAAATTTCTTCAACGGACTCAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: LP_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from larvae and early pupae.

    (Rubin et al., 2000)
    Sample preparation

    Larvae and early pupae at various stages of development were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).

    (Rubin et al., 2000)
    Comment
    Reported As
    Symbol Synonyms
    LP
    (Rubin et al., 2000)
    LP_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)