FB2026_03 , released September 17, 2026
Clone: Dmel\RH38575
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General Information
Symbol
Dmel\RH38575
Species
D. melanogaster
Name
RH38575
FlyBase ID
FBcl0255826
Feature type
cDNA_clone
Computed gene(s)
Dmel\vptr
 
Collection Status
BDGP
Known Problems
contains transposon sequences reverse transcriptase error
FlyBase assessment
  • reverse transcriptase error
Library
RH_cDNA
  • RH cap-trapped cDNA library of D. melanogaster, iso-1 strain, adult head.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage
    adult head
    (Stapleton et al., 2002)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    2,871
    NCBI
    BT003455
    Full Length sequence dataSequence Downloader
    GACGTGGCATCGTTTCCACTAGTTGGCCGGCTTCAGTTTTCGCAAGAAACTACGAAAGTTTCCCTCCAGCAGAGTGTACA
TATTTTTACACCCGATGTGCAACAACCAAATACAAAATCGCTAATTCTTATATTCGGTTTGGGTTTCGACTGCCTATTCG
TTATTGCCTGCGACTTAGCTTTCGCGAAGGCAACTTGTTGCTTCAAAGCGATGGTTTCAGTAGCTGTTGAGTTACGGCAC
CATGAACCAGTATAGTTGGAAGCAGTTTTTGGAAAATAATAATCTACTGGGCACAGCCGGAGCGGGTCAGTTGAAAGCTC
AAATAGTACCCGCCCAGCCAAAGACGCTCCAGCACCTCCCCAAGCGACCAGCGGTGGACCTGGCCTTTCACAACCTGACG
TACCGCGTCAAGGAGGGGAACCGGAGCAATGCCAAGACCATTCTGAAGGGCGTCAGTGGACGCCTCCGGTCTGGAGAACT
CACCGCCATAATGGGACCATCGGGTGCTGGCAAGAGTACGCTGCTCAACATTTTGTCTGGCTACAAAACTTCGAGCATCG
AAGGCAGCGTTACGATGAACGGTGCCGAGCGCAACCTAAGCGCATTTCGCAAGCTTTCTGCCTACATCATGCAGGACAAT
CAGCTTCACGGAAACCTTACCGTCCAGGAAGCAATGACTGTGGCAACGAATCTGAAGCTCAGCAAGAAGTTTTCGAAGCC
CGAGAAGCATTCAATGATCGATGACATTTTGCTTACTCTCAGCCTGAGCGAGCACCGATATACGATGACTCGAAATTTGT
CCGGCGGTCAGAAAAAGCGGCTCTCGATTGCCCTTGAACTGGTCAGCAATCCTCCCATAATGTTCTTTGACGAACCCACA
TCCGGCCTGGACAGCTCTACCTGTTTTCAGTGCATTCATCTGCTAAAAATGCTAGCTGCTGGCGGACGCACAGTCATATG
CACCATCCATCAACCGTCCGCTCGTCTCTTCGAGATGTTCGACCAGCTGTACACTCTCGCAGATGGACAGTGCGTTTATC
AAGGCAGTACAAAGCAACTGGTGCCCTTCTTGTCTACGCTAAACCTGGAGTGCCCCAGTTACCACAATCCGGCAAGCTAC
GTGATTGAGGTGTCCTGCGGGGAGCACGGAGATCATACGCGCAAACTGGTCGATGCTATTGACAACGGCAAGCGGGACGT
TCGTTCATCAGCAGACTATGCCGGCCTCAAGGCTCGCAACGATTTGGTAAAGGTGCAAAACCTGAAGGCTATTCTCGATA
AGAACGACGCTTCAAGTGTCAGCGGTCGCAAGTACGAGGACAATTTGACCTTGAACAACGGTTTGCTCAACGGCATGGTC
AATGATATTGTGAAGGAGGGCAACGTTAGCTCTGCCTTGGTCACTACCAATGAAAGGGGAGATGCCATGATCGATGTGGA
GAAGTCCGTAAACTGCACCACTGCCTTGCTTACGGAGGAGATAACATCACCAGAGCGATATCCGACATCGCAGTTTCACC
AGTTCTGGGTGGTTCTTAAGCGAACTTTACTCTTCAGTTACCGGGATTGGACTCTTATGTATCTGCGTTTATTTGCCCAT
CTGTTAGTTGGATTTTTGATTGGGGCACTCTACTATGACATTGGCAATGATGGAGCAAAAGTTTTGAGCAACCTGGGATT
CCTATTCTTTAACATGCTTTTCCTCATGTATACATCTATGACTATTACGATCCTATCATTCCCGCTTGAGATGCCCGTTT
TGTTGAAGAAAACTTTAATCGCTGGTACTCGCTAAAGTCCTACTATTTGGCTATATCAGTTGCTGATTTACCGTTTCAAG
CTATTTTCTGCGTTATCTACGTGTCGATCGTGTATTATTTCACTTCACAACCTTGGGAGCTATTCCGCTTTTCCATGTTC
TTGTCGGCCTGCCTTCTCATTTCTTTCGTTGCTCAGTCCGTGGGTCTGGTGGTAGGCGCTGCAATGAATGTTCAGAACGG
TGTCTTCCTGGCCCCAGTGATGTCCGTGCCCTTCCTTCTGTTCTCCGGCTTCTTTGTCTCCTTTGACGCTATACCAGTAT
ACTTAAGGTGGATCACATATTTGTCGTACATACGATATGGATTCGAGGGCACAGCTCTGGCCACCTACGGTTACGGACGG
GAGAAACTTAGGTGCTTCCAGACTTACTGCCATTTTAAATCACCTATTACCACGCTGGAGGAACTGGACATGGTTAACGC
TAACTTTACACTCGACATTGTGGCCCTAATTGTGATCTTCGTGGTGCTGCGCATCTCGGCATACCTGTTCTTGCGCTGGA
AGCTGAAGACTGTGCGATAAGCATATTACATAATTTTAAATGTTGTTTTGGATTTAATAGCTTTCTATGTACGTACTTTA
TATTGTAGTTTTCAAAAAAGCGTCTTGGGTTTTGTTCTTAATCATTAATCTTTTACAAATTTCTTTTTGTCTAGTTTTTA
GTCGCGAAATTCTTCTTTTAAGTGATACAAACGCAAGGAGGTTTCTTATCTTAAATCAGCTTGATTCCAGCCGCATATGT
AGTAAAACCAATTCAAACCACTGTTGGAGCACATCATTGATATAAGTGAATCGTATAATGAAATAAGTTATTGAACGTGA
AACCAATTTTCTAAATAAAAATAACTGAATAACATTTCCAATTGTATTTACCGAAGGATTTTTGAATGACTTTAAATTAA
AAACGAACATGTTTTGTGTCTATATACATTAATATTTTTATTCAAGGTACTTTAAGACCCTTTGGAATTTTTTGTAATAT
TTTCTTATTACAGGTTTAGAATAAACATTTTTGAATATATACTGTAAAAAAAAAAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: RH_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

    (Stapleton et al., 2002)
    Sample preparation

    For the RH cDNA library, adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA was polyA+ selected twice (RNA made by L. Hong). cDNA was synthesized by priming with the oligo(dT) primer adapter (5'-GAGAGAGAGAGGATCCAATACTGGAGAGTTTTTTTTTTTTTTTTVN-3'). The first strand was synthesized in presence of trehalose, which increases the full-length cDNA synthesis (Carninci, P. et al. 1998. Proc. Natl. Acad. Sci. U.S.A. 95(2): 520-524; Carninci, P. et al. 1999. Methods Enzymol. 303: 19-44). Full-length cDNA was selected with the biotinylated cap-trapper (Carninci, P. et al., Genomics 37: 327-336). A linker was then ligated to the single-strand cDNA following the published protocol (Shibata, Y., et al., Biotechniques, in press). Subsequently, the cDNA was normalized by using RoT=1.0 as published (Carninci, P., et al., Genome Res. 10: 1617-1630). Second strand cDNA was primed with the (5'-AGAGAGAGAGCTCGAGCTCTAATAAGGTGACACTATAGAACCA-3') primer. After restriction digestion of the hemimethylated cDNA with BamHI and XhoI, thecDNA was cloned in the lambda FLC-I vector. Subsequently, the library was bulk-excised into pFLC-I plasmid as described (Carninci, P., et al., Genomics, 2001, 77:79-90 ). cDNAs were transformed into DH5-alpha TonA strain.

    (Stapleton et al., 2002)
    Comment
    Reported As
    Symbol Synonyms
    RH
    RH_cDNA
    Riken head library
    References (1)
    Research paper (1)
    Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
    The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
    External Crossreferences and Linkouts ( 2 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)