FB2026_02 , released June 18, 2026
Clone: Dmel\SD12738
Some tools are temporarily unavailable. We are completing maintenance and working to restore these tools as soon as possible. Thank you for your patience.
Open Close
General Information
Symbol
Dmel\SD12738
Species
D. melanogaster
Name
SD12738
FlyBase ID
FBcl0278030
Feature type
cDNA_clone
Computed gene(s)
Dmel\TyrRS
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    SD_cDNA
    • SD cDNA library of D. melanogaster, Schneider's Line-2 cells.
    Strain
      Vector
      Tissue Source
      Sequence Data of the Insert
      5' Sequence
      Total bases
      567
      NCBI
      BG641406
      5' sequence dataSequence Downloader
      CGTGGGCAAGGTCGTCGAGGTGGCCCGTCATCCAGATGCCGATACTCTATATGTGCTGAAGATAGACTTGGCTGAGGCGC
AACCCAGAACGATTATTAGTGGCCTGGTGAAGTTTGTCACCGAGGAGGAGCTGAACCAGCGATTGGTGGCGGTTCTTTGC
AACCTGAAGCCGTCCAAGATGCGCGGCATCTTGTCCGAGGGCATGGTTCTGTGCACCTCCAACGCTGACCACACGGTGGT
GGAGCCTATTGTGCTGCCAGCCACTGCTACTGCTGGCAGTCGCCTCTCCTTCGAGGGATTCAGTGGCACGCCCGATGAGC
AGCTTAATCCCAAGAAGAAGGTGTGGGAGAAACTCTCCGCCGACTTCAAGACGAACAGCGATGGATTGGCCGTGTGGAAG
GACAACTTCCTGCTGACGCCCGAGGGCGAAAAGCTTTCCAGCAAGCTTGCCAACTGCTCCATTAAATAAAACTTTCAACG
CCAACCTACCTGAGCCGTGTAATACTTACAACTAAGCCGAATGCTCACAAAACAGAAATTGCTGCTAATTATTGATTCCT
CTTCGTA
      Library Information (1)
      Library: SD_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from cultured cells; cell line used was not derived the iso-1 sequenced strain.

      (Rubin et al., 2000)
      Sample preparation

      For the SD cDNA library, Schneider L2 cells were collected.

      Protocol

      RNA was poly(A)+ selected once. oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~300,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).

      (Rubin et al., 2000)
      Comment
      Reported As
      Symbol Synonyms
      SD
      SD_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)