AGCCATCCAGTGCGGTTGGATGAGCTCCACCAAAGGAATACGAAAAGAACAGTGAAAAAAGTGAAATACAGCAGATAAGA AAGTGAAATACATACATAAGTGAACCGGGAAAGAAAGAGATAGAACCGGAAGTGGTCCGTGGAGAAAGGACTGTCGTTAT CGCAGTGCGGGTGCTTGAACTCTCGGCACAATGGGGCACGGAGTGGCCTCCATTGGGGCTCTGCTAGTAATCCTGGCCAT CTCCTATTGCCAGGCGGAGCTAACGCCACCATATTTCAATCTGGCCACGGGCAGGAAGATTTATGCCACAGCTACGTGTG GCCAGGATACGGATGGACCAGAGCTCTACTGCAAACTAGTGGGAGCCAATACGGAACACGATCACATCGACTATTCAGTT ATCCAGGGACAGGTCTGCGATTACTGCGATCCCACGGTGCCGGAGAGGAATCATCCGCCAGAGAACGCGATCGATGGCAC CGAGGCCTGGTGGCAGAGTCCTCCGTTGTCCAGGGGCATGAAATTCAATGAAGTCAATTTGACCATCAACTTCGAACAGG AATTCCATGTGGCCTATTTGTTCATTCGCATGGGCAACTCCCCTCGTCCGGGTCTCTGGACGCTGGAGAAGTCCACGGAT TATGGCAAGACCTGGACGCCTTGGCAGCACTTTTCCGATACCCCCGCCGATTGTGAGACGTACTTTGGCAAGGACACCTA CAAGCCAATTACCCGCGACGACGACGTCATCTGCACCACTGAATACTCAAAGATTGTGCCGCTGGAGAACGGCGAGATTC CCGTGATGCTGCTCAATGAGCGACCCAGTTCCACCAACTACTTCAACTCAACGGTGCTGCAGGAATGGACTCGTGCCACT AACGTCAGGATTCGTCTGCTTCGCACCAAGAATCTTTTGGGACATTTGATGTCTGTGGCTCGACAAGATCCCACGGTGAC GCGTCGCTACTTCTACTCGATCAAGGACATCTCGATCGGAGGAAGGTGCATGTGCAATGGACACGCCGACACCTGTGACG TCAAGGACCCGAAGAGCCCAGTCCGCATCCTCGCATGCCGTTGCCAGCATCACACGTGCGGCATCCAGTGCAACGAGTGC TGTCCTGGATTCGAGCAGAAAAAGTGGCGCCAAAACACCAATGCCCGACCATTCAACTGCGAACCCTGCAACTGTCATGG TCACAGCAACGAGTGCAAGTACGATGAGGAAGTGAACCGCAAGGGACTGTCCCTGGACATTCACGGACACTACGATGGTG GTGGTGTTTGCCAGAACTGTCAGCACAATACCGTGGGAATTAACTGCAACAAGTGCAAGCCGAAATACTATAGACCCAAG GGCAAGCACTGGAACGAGACCGATGTCTGCAGCCCCTGCCAATGCGACTACTTCTTCTCCACCGGACACTGCGAGGAGGA GACCGGAAACTGTGAGTGCCGTGCTGCCTTCCAACCGCCAAGTTGCGACTCCTGCGCGTATGGATACTATGGTTACCCCA ATTGCCGGGAGTGCGAATGTAATCTGAATGGAACCAATGGCTACCACTGCGAGGCTGAAAGCGGACAGCAGTGCCCGTGC AAGATCAATTTCGCGGGGGCCTACTGTAAGCAGTGCGCCGAAGGATACTACGGATTCCCCGAGTGCAAGGCATGCGAGTG CAACAAAATCGGCTCGATTACCAACGACTGCAACGTGACCACTGGAGAGTGCAAGTGCTTGACCAATTTCGGAGGCGACA ACTGCGAGCGCTGCAAGCACGGATACTTCAACTACCCTACTTGCTCATACTGCGACTGCGACAACCAGGGCACGGAGTCG GAAATCTGCAACAAGCAGTCTGGCCAGTGCATCTGTCGTGAAGGTTTTGGAGGCCCAAGATGCGATCAGTGCTTGCCAGG ATTTTACAACTATCCGGACTGCAAGCCATGCAACTGCTCCAGCACGGGTAGTTCGGCGATCACCTGCGACAACACGGGCA AATGTAACTGCCTCAACAACTTTGCCGGGAAACAGTGCACGCTCTGTACTGCCGGATACTACAGCTATCCGGATTGTCTA CCTTGTCACTGTGACTCTCACGGATCTCAGGGAGTGTCCTGTAATTCAGACGGACAGTGCTTGTGTCAGCCCAACTTTGA CGGACGTCAGTGCGATTCCTGTAAGGAGGGCTTCTACAACTTCCCCAGCTGCGAGGACTGCAACTGTGATCCTGCAGGAG TGATCGATAAGTTCGCCGGATGCGGATCGGTGCCCGTGGGTGAGCTCTGCAAGTGCAAGGAGCGAGTGACGGGCAGGATC TGCAATGAGTGCAAGCCATTGTACTGGAACCTGAACATCAGCAATACTGAGGGATGTGAGATTTGCGATTGTTGGACAGA TGGTACTATTTCCGCACTGGACACCTGTACCTCGAAGTCCGGACAGTGTCCCTGCAAGCCGCATACTCAAGGCAGGCGCT GCCAGGAGTGTCGGGATGGTACCTTCGATCTGGACAGTGCCTCGCTGTTTGGCTGCAAGGACTGCAGTTGCGATGTGGGC GGCTCGTGGCAAAGTGTTTGCGACAAGATCAGCGGACAGTGTAAGTGCCACCCGAGGATTACGGGTCTGGCATGTACCCA GCCACTGACCACCCACTTCTTCCCAACGCTGCATCAGTTCCAGTACGAATACGAGGATGGATCACTACCATCGGGCACTC AGGTGCGCTACGACTACGATGAGGCGGCATTCCCCGGATTTAGCAGCAAGGGCTATGTGGTGTTCAACGCCATTCAGAAC GATGTACGGAACGAGGTCAATGTCTTTAAGTCATCTCTGTATCGTATTGTCCTGCGATATGTGAATCCAAATGCCGAGAA TGTCACTGCCACCATTTCGGTTACTTCAGACAATCCTCTGGAGGTTGATCAGCACGTTAAGGTTCTACTGCAACCCACAT CGGAACCTCAGTTCGTAACTGTTGCGGGTCCCTTGGGAGTGAAACCCTCAGCCATTGTTTTGGATCCGGGTCGTTATGTA TTTACCACCAAGGCCAACAAGAATGTGATGCTTGATTACTTTGTTCTCCTACCCGCCGCTTACTACGAAGCCGGAATTCT AACGCGCCACATCTCAAATCCCTGTGAACTGGGCAACATGGAGCTGTGCCGTCACTACAAGTACGCCTCTGTGGAAGTGT TCTCACCAGCGGCAACTCCATTCGTCATCGGTGAAAACTCCAAGCCGACCAATCCCGTCGAGACGTACACTGATCCGGAG CATCTGCAGATTGTGAGCCATGTCGGTGATATTCCCGTACTGAGCGGATCCCAAAATGAGCTGCACTACATCGTGGATGT GCCACGAAGTGGCCGGTATATCTTCGTGATTGACTACATCAGCGATAGGAACTTCCCCGACAGTTACTATATCAACTTAA AACTAAAGGATAATCCAGATTCGGAAACATCCGTGCTGTTGTATCCATGCTTATACTCGACTATTTGCCGAACATCAGTT AACGAGGATGGTATGGAGAAGTCATTCTATATTAACAAAGAGGATTTGCAGCCAGTCATCATCTCCGCTGACATCGAAGA TGGTTCTCGTTTCCCGATTATCTCGGTGACAGCAATTCCCGTCGACCAATGGTCCATTGACTACATCAACCCAAGTCCCG TCTGTGTGATCCATGATCAGCAATGCGCCACTCCGAAATTCCGTTCCGTTCCCGATTCCAAGAAGATCGAGTTCGAAACT GATCATGAGGACCGTATTGCCACCAACAAACCTCCATATGCCTCACTCGATGAACGAGTTAAGCTGGTCCACTTAGACAG TCAAAATGAAGCAACCATCGTGATTGAATCAAAGGTTGATGCTACGAAACCGAATCTATTTGTGATCCTGGTGAAGTACT ACCAACCGAGCCACCCCAAATACCAGGTGTACTACACGCTGACAGCGGGCAAGAACCAGTACGATGGCAAGTTCGACATT CAGCACTGTCCATCGAGTTCCGGATGCCGCGGTGTGATTCGCCCCGCCGGAGAGGGTTCATTCGAGATTGACGACGAGTT CAAGTTCACGATCACGACCGATCGATCCCAGAGCGTTTGGCTGGACTACCTGGTGGTGGTTCCCTTGAAGCAGTACAATG ATGACCTACTGGTGGAAGAGACCTTCGACCAGACCAAGGAGTTCATTCAGAACTGCGGCCATGACCACTTCCACATAACC CACAATGCCAGTGATTTCTGCAAGAAATCCGTTTTCTCTCTAACAGCCGATTACAATAGTGGAGCATTGCCTTGCAATTG CGACTACGCCGGATCCACGAGCTTCGAGTGTCATCCATTCGGAGGTCAGTGCCAGTGCAAGCCGAATGTGATTGAGCGCA CGTGCGGAGCCTGTCGCAGTAGATACTACGGATTCCCCGACTGCAAGCCATGCAAGTGTCCCAACAGCGCCATGTGTGAG CCCACCACTGGTGAGTGCATGTGTCCGCCCAATGTCATTGGGGATTTGTGCGAGAAGTGCGCCCCCAACACCTATGGATT CCATCAGGTCATTGGCTGCGAGGAGTGTGCATGCAATCCTATGGGCATTGCCAATGGAAACTCCCAGTGCGATCTGTTCA ATGGAACCTGCGAGTGTCGGCAAAATATCGAGGGCAGGGCCTGTGATGTTTGCTCCAATGGATACTTTAACTTCCCGCAC TGCGAGCAGTGCAGTTGCCACAAGCCCGGCACCGAACTGGAGGTGTGTGACAAGATCGATGGCGCATGCTTCTGCAAGAA GAATGTGGTTGGAAGGGACTGTGACCAGTGTGTCGATGGAACATACAATCTGCAGGAGTCGAATCCGGATGGTTGCACCA CTTGCTTCTGCTTTGGCAAGACTTCGCGATGCGACAGTGCCTATCTCAGAGTTTACAATGTGAGCCTGCTTAAGCATGTG TCCATCACTACTCCGGAATTCCACGAGGAGAGCATCAAGTTTGATATGTGGCCCGTACCCGCGGATGAAATTCTTCTGAA TGAGACCACGCTGAAGGCAGACTTTACCTTACGGGAGGTCAACGATGAACGACCTGCGTACTTTGGGGTTCTGGACTATC TGCTTAACCAGAATAATCACATCTCTGCTTATGGCGGAGACCTGGCTTATACTTTACATTTCACTTCTGGCTTCGACGGC AAATACATTGTGGCTCCAGATGTAATTTTGTTCAGTGAACACAATGCCCTGGTCCATACAAGCTATGAGCAGCCCAGCCG AAATGAACCATTCACCAACCGCGTCAATATAGTGGAATCGAATTTCCAGACGATTTCCGGAAAACCAGTTTCACGAGCTG ATTTCATGATGGTTCTGCGGGATCTAAAAGTAATCTTTATCAGAGCCAACTATTGGGAACAGACCTTGGTGACCCACCTG TCCGATGTGTATCTAACTCTAGCCGATGAGGATGCAGATGGCACTGGCGAATACCAGTTCCTGGCCGTGGAGCGTTGCTC CTGCCCACCTGGTTACTCCGGACACTCGTGCGAGGACTGTGCCCCGGGCTACTACCGAGATCCCAGCGGACCTTATGGTG GTTACTGCATTCCCTGCGAGTGCAATGGACATTCGGAGACCTGTGATTGCGCTACCGGAATCTGTTCAAAGTGCCAGCAT GGAACAGAGGGTGATCACTGTGAGCGCTGTGTGTCCGGTTACTATGGAAACGCCACGAATGGAACACCCGGAGATTGTAT GATCTGCGCTTGCCCACTGCCATTTGACTCGAACAACTTTGCCACCAGTTGCGAAATCTCCGAGAGCGGAGACCAAATCC ATTGTGAGTGCAAGCCAGGATATACAGGACCACGTTGCGAGTCTTGCGCCAATGGTTTCTATGGAGAGCCAGAGAGCATA GGAGAGGTGTGCAAGCCGTGCGAGTGTTCCGGTAACATCAATCCAGAGGATCAGGGATCTTGCGACACTAGGACCGGTGA ATGTCTTCGCTGCTTGAATAACACCTTCGGTGCTGCCTGTAATCTGTGTGCACCTGGATTCTATGGCGATGCTATAAAGC TAAAGAACTGCCAGAGCTGCGACTGCGATGATCTGGGCACCCAGACGTGTGATCCCTTTGTGGGTGTCTGCACCTGTCAC GAGAACGTCATTGGCGACCGTTGCGATCGCTGTAAGCCGGATCACTATGGATTTGAGTCAGGTGTTGGGTGCCGAGCCTG CGACTGCGGTGCTGCCTCCAATTCAACGCAGTGTGACCCACACACAGGTCACTGTGCCTGCAAGTCGGGAGTGACAGGAC GACAGTGCGATCGATGCGCCGTTGACCATTGGAAATACGAAAAGGACGGTTGCACTCCGTGCAATTGCAATCAGGGATAC TCACGAGGATTCGGTTGCAATCCCAACACCGGCAAGTGCCAATGTCTGCCAGGAGTAATTGGAGATAGATGCGACGCTTG TCCGAATCGATGGGTTCTGATCAAGGATGAAGGCTGTCAGGAGTGCAACAACTGCCACCACGCCCTTTTGGATGTCACTG ATCGAATGCGCTATCAGATCGATAGTGTCCTGGAGGACTTTAACAGTGTAACCCTGGCCTTCTTCACCAGTCAAAAATTG AACTATTACGACCAGCTGGCCGATGAACTGGAGCCCAAGGTTAAGCTATTGGATCCCAACAGCGTGGACCTGAGTCCATC CAAAAAGGCCAACAGCGAATTGGAATCCGATGCCAAGTCCTATGCCAAACAGGTCAACCAGACCCTGGCCAACGCCTTCG ATATCCGTGAGCGATCGAGCACCACTTTGGGTAACATAACAGTTGCTTATGATGAGGCGGTCAAGAGTGCCGACCAGGCC AAAGAGGCCATCGCTTCGGTGGAAGCTCTTTCCAAGAATCTCGAGGCGGCAGCGAGCACAAAGATTGATGCTGCTCTGGA GCAAGCACAGCACATTTTGGGACAAATCAACGGAACCAGTATCGAACTTACACCCAATGAACAAGTCCTGGAAAAGGCGA GGAAACTCTATGAAGAAGTGAACACTCTAGTTCTGCCCATCAAGGCGCAAAACAAGAGCCTCAATGCCCTCAAGAATGAT ATTGGAGAATTCAGCGACCATCTGGAAGATCTTTTCAACTGGAGCGAAGCTTCACAAGCCAAGTCCGCTGACGTTGAGCG CCGCAATGTGGCCAACCAGAAGGCCTTTGACAACTCTAAATTCGACACCGTTTCGGAGCAAAAGCTACAGGCAGAGAAGA ACATCAAAGATGCTGGCAATTTCCTCATCAATGGCGATCTGACCCTGAATCAAATTAATCAGAAGTTGGATAACTTGAGA GATGCTCTAAACGAGCTCAATTCGTTCAATAAAAATGTGGACGAAGAATTACCCGTGAGGGAGGACCAGCATAAGGAGGC GGATGCCCTTACCGATCAAGCCGAACAGAAGGCGGCCGAATTGGCCATTAAGGCCCAAGATCTGGCTGCCCAATACACGG ATATGACCGCCAGTGCGGAGCCGGCCATCAAGGCCGCAACTGCCTACTCGGGAATCGTTGAGGCCGTTGAAGCTGCCCAG AAACTCAGTCAGGATGCAATCTCTGCGGCCGGAAATGCCACTGATAAGACAGATGGCATTGAAGAGAGAGCTCATCTCGC TGACACTGGATCTACTGATCTTCTCCAGAGAGCACGCCAGTCTCTGCAGAAGGTACAAGACGACCTAGAGCCCCGTCTGA ACGCTTCGGCCGGCAAGGTGCAGAAAATCTCAGCCGTGAACAATGCCACCGAACACCAGCTGAAGGATATTAACAAGCTG ATTGACCAACTGCCAGCTGAGTCGCAGAGGGATATGTGGAAGAATTCAAATGCCAATGCCAGTGATGCTCTGGAGATACT GAAGAACGTCCTAGAGATACTCGAACCTGTGAGTGTCCAAACGCCAAAGGAACTCGAGAAGGCACATGGCATCAATAGAG ATTTGGATCTGACCAATAAGGACGTTTCTCAAGCTAACAAACAATTGGATGACGTAGAAGGATCCGTTTCGAAGCTAAAC GAATTGGCAGAGGATATCGAGGAACAGCAGCACCGTGTGGGCAGTCAGAGCCGGCAGTTGGGCCAGGAAATCGAGAACTT GAAGGCCCAAGTGGAGGCTGCTCGCCAGTTGGCCAACAGCATCAAGGTGGGCGTCAATTTCAAGCCAAGCACGATCCTGG AACTGAAGACACCGGAAAAGACCAAGTTGCTAGCCACTCGCACCAATCTATCGACCTATTTCCGCACCACCGAGCCATCC GGTTTCCTTTTATATCTGGGCAATGACAACAAGACCGCCCAGAAGAACAACGACTTTGTGGCCGTTGAGATTGTGAATGG CTATCCGATTCTTACCATAGATTTGGGCAATGGGCCGGAACGCATCACCAGCGATAAGTACGTGGCAGATGGTCGCTGGT ATCAGGCTGTTGTGGATCGCATGGGTCCGAATGCCAAGCTAACAATTAGGGAAGAACTACCCAACGGTGACGTGGTTGAG CACAGCAAGTCTGGCTATCTTGAAGGATCCCAGAATATCCTTCATGTGGATAAGAACAGTCGCCTATTTGTTGGTGGCTA TCCGGGTATTTCGGACTTCAATGCTCCGCCAGACTTGACCACTAACTCCTTCTCCGGCGACATTGAGGATCTAAAGATTG GCGATGAGAGCGTAGGATTGTGGAACTTTGTATATGGCGATGATAACGACCAGGGCGCCAGGGAGCGGGATGTGCTGCTC GAGAAGAAGAAGCCGGTAACTGGTCTGCGGTTCAAGGGTAATGGATACGTCCAACTGAACGCCACATCGAACTTGAAGAG CCGCTCCAGCATCCAGTTCAGCTTCAAGGCAGACAAGGATACTTCCAATGGACTGCTGTTCTTCTACGGCAGGGATAAGC ACTACATGAGCATTGAGATGATTGACGGAGCTATCTTCTTCAACATTAGTTTGGGTGAAGGTGGAGGCGTTCAATCCGGC AGCCAGGATCGCTACAATGACAACCAATGGCACAAGGTCCAGGCGGAAAGGGAAAACCGAAACGGCCTTCTCAAGGTCGA CGATATAGTAATATCACGGACCAATGCTCCATTGGAAGCGGACTTGGAGTTGCCCAAGCTGAGGAGATTATACTTCGGTG GTCATCCGAGACGTCTGAATACCTCGATAAGTTTGCAACCCAACTTTGATGGCTGCATCGATAATGTTGTGATCAACCAG GGAGTGGTCGACCTTACAGAATACGTAACTGGCGGCGGAGTTGAGGAAGGATGCTCGGCGAAGTTCTCCACGGTGGTGTC ATATGCGCCGCATGAGTACGGATTCCTGAGGATGAACAACGTTTCCTCAGATAACAATCTGCATGTGGTTCTGCACTTCA AGACCACGCAACCGAATGGCGTACTCTTCTATGCCGCCAATCATGACCAGAGTTCCACCATTGGCCTCAGCCTACAAGAT GGTTTGCTCAAGCTCAACAGCATGGGCAGTCAGTTGGTAATCGACGATCGTATTCTGAACGATGGCGAGGATCATGTGGT TACAGTTCAGCACACCCAAGGTGAACTTCGTCTTACGGTCGATGATGTAGATAACAAGAGACTTGGTTCTCCACAACCAT TAATCCTGGAGGGTGGTGACATCTTCTTTGCTGGCTTGCCGGACAACTATCGAACACCGAGGAACGCGTTGGCCTCTTTG GCCTACTTTGTCGGTTGCATCAGCGATGTAACCGTGAACGAGGAGATCATTAACTTTGCCAACAGTGCTGAGAAGAAGAA TGGCAACATCAACGGTTGCCCACCACATGTACTAGCCTACGAACCAAGTTTGGTGCCCAGTTACTACCCAAGTGGAGACA ACGAGGTGGAGTCTCCGTGGTCAAACGCCGATACTCTGCCCCCACTTAAGCCAGATATTGAAAGCACACTACCGCCCACA ACGCCCACTACGACCACCACGACAACTACCACAACAACATCGACTACTACAACTTCAACAACGACAACTACTACGACACC ATCCCCGATAGTCATTGATGAGGAGAAGGAGATTGAGGCGAAAACACCGCAAAAGATCCTAACAACGCGGCCACCAGCCA AGCTGAACCTTCCCAGCGATGAGCGCTGCAAGTTGCCGGAGCAACCCAATTTCGATGTTGATTTCACAGAGGCGGGTTAC CGATTCTATGGACTGCGGGAGCAGCGCCTGCAGATTAACTCACTGCCGGTGAAGGTACGCCGTCACCATGACATTGGCAT CTCATTCCGTACTGAGCGACCAAACGGTCTGCTCATTTATGCCGGAAGTAAGCAGCGCGATGACTTCATAGCCGTGTATC TTCTGGACGGTCGGGTGACATACGAGATACGGGTGGGTGCACAGTTGCAGGCCAAGATCACTACCGAGGCGGAGCTGAAC GACGGCACCTGGCACACCGTGGAGGTGGTGCGGACCCAACGCAAGGTATCCCTGCTCATCGACAAGTTGGAGCAGCCGGG TTCCGTGGATCTGAATGCGGAAAGATCCGCTCCGGTATTGGCTGTCGAGTTGCCCATCTATCTGGGCGGAGTTAACAAGT TCCTGGAGTCGGAGGTTAAGAATCTCACCGATTTCAAGACTGAAGTACCGTACTTTAACGGCTGCCTCAAGAACATCAAG TTCGACGCTATGGATCTGGAAACACCACCGGAGGAGTTTGGAGTGGTTCCGTGCTCTGAGCAGGTGGAACGCGGTTTGTT CTTCAATAACCAGAAGGCGTTTGTAAAGATCTTTGATCACTTCGATGTGGGCACTGAGATGAAAATCAGCTTCGATTTCC GACCGAGGGATCCCAACGGATTGCTCTTCTCTGTGCACGGCAAGAACTCTTATGCCATACTCGAGCTAGTAGACAATACC TTGTACTTTACCGTGAAGACCGATCTGAAGAACATTGTGTCCACCAACTATAAGCTGCCCAACAACGAAAGCTTCTGCGA TGGCAAGACGCGCAATGTCCAGGCCATCAAATCCAAGTTTGTGATCAACATAGCCGTGGATTTCATTAGCTCCAACCCCG GCGTGGGCAACGAAGGATCAGTGATCACAAGGACCAATCGTCCGCTGTTCTTGGGCGGACATGTGGCCTTCCAGAGGGCC CCGGGCATCAAGACCAAGAAGTCCTTTAAGGGCTGCATCAGCAAGGTGGAGGTTAACCAAAGGATGATCAACATCACGCC GAACATGGTCGTTGGCGATATTTGGCAGGGCTATTGTCCCCTCAACTAATAAAGAACTTCGGAACGACTTAAGGACCTAT GATTTATTTGGACAACGACTACGAAAAACTGCCAGGAAGAATCTTCTTTTGCCGACAACTGCTAATCAAAGGACAACGAA CGATCAAATTTCAAACGAACAAAATCGACGGGTCATAAAAAAGTCATAAGGTCTTCAAAGTAATAGATCCTTAGCAAAGA TGCCAGTCGTATATATTCCTAGTACTATAAGCCAAACTGCGTGCCAATTCTATTAAAGCCATTTACATTTATATATCCTT TTTAAAACTCCTTAAGAGAATCTTGCATTTTTGTAATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from cultured cells; cell line used was not derived the iso-1 sequenced strain.
For the SD cDNA library, Schneider L2 cells were collected.
RNA was poly(A)+ selected once. oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~300,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).
