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FB2026_01
,
released March 12, 2026
AAAAAATGTAAATTGTGCAGCAAGTGAAATTGAAAAGCAATATATTTAAGTGTGATCTGTGTGTTGTGGTGTGTGGGTGT GTGTTCGACATACAGCTAATAGGCGAGCGGGCGACGGCGCCGCACCCGCCTCGCCACGCCCCCATCGGCGAATCGAAAAA GAGGCGAAGCAACGCGATGGCCTCCCAACATTCCAGTTGGCGTTTTGGCAAGCGGAGCAAATTACAATTACGCATCAAAG TTTCGCAGGATCCGGAGGACTTCTACCGCCTGGATCCACAGCGATCGGCCGCCGAAAATGCATTCGAAATATACTCGATG CTCTGTTTGGAAGAGACGCGGGACACGAAGCGCCTGTTTCTGCTCAACGCGCTGGCCTCGCTCTGTTTGGGGGCCCGAAA GGGCTCCCACCACAGCAATATACGCTTCACCACCGAGGAGCTGCTCTACTGCCTTAGTGCCTCACTGGTGCACACCTTCA CCCAAGTGCGAGCGGCGGCACTGCGAACGATCCGGTATGCCATTAGCACGCCCAATGACATCAAGACGTTCAATGCACTG CAGCTGCAGCACCTGCTCTGCCGCTCCATCGATCTAATGCTGAAGAACGACGACGAGCGCGTCCAGGCACTGAAGCTGGT CCGCAAGATGCTGGCCATCGCGCCGGAGGACATCAGTCCGGCGGTGGTGCGCTGCCTGGTCTCGCTGGCGGATAGCGGCA TCGAGGAGAACGACAATCTGTTGCGTGCCTGCCTGGCCACCCTGGCAGAGTTCGCTGTGCTCAATCCGGCGCTGCTGATC GTCTGCGGCGGCGTCACCTCCATAACCCGCAATGTGCTAGAGTGCCACAATCCCCGCATCGCTGAGAGCCTGTGCGGCGT GCTTCTGTACCTGCTCGAGTGGCCGCAAACGAGGAACATCTGCGGTGTGCGGCTGGACTGCCTGGCAGCTCCTTATTGCG ATTTCACCTACCGGCAGAGTATCGTCGATAAGAACAAGGATGCGCGCGAACTTCGCTACACCAGCTGTCGGCTGGCACTG CTGTCTGTGCTGCGCAGCTGGACGGGCACCCTCGAATTCTGTGATCCTAGCAAACCCTCAGGCCTGAAAGCAATAGTCGA CGCTCTGTATTTGAACCAGATCGAAGTCAGAAAAGCAATATTGGATTTGCTGTACGAGCTGCTCACGCTGCCGCAGCCAA CATGGACGGATGACTATGCGGTGGCGTTGCAGGCTGTGGATCCGTCGGACTTCCAGGACACGTGGCTGCTGAGCAATGGC TTCGTGTCCGCCGAAGGGCGCTCCATTTTGCCAACGCTGGCGGCGCGTGCACCGAGCGTAGTGGAGCAGCACCTGGCGCT GATGCTGTACTGTTTCCTCGAGACTGGACTGCTCAATGCGCTCGTCGAGGTTGTGGTGAGCAGCGATCAGTTCGTCTCGG TACAGGCTACGGTGCTGATTGGCAAGATCCTGCAGCTAATGCACACCCACCTGCCCCCGGATATTTGCTGTACGAGTCCG GCACTGCCCACGCTGGTGAGTCATGCCACATTGGGGAACCAGCAGGCCAATGCAGCGGTGGCCGCTCTGCAAAACTACCA GAAGTTGCTGCGCCAGCGGCCGGCGTCGCGCAGCTTATTCCTAGACAGCATTATCCAGGGCGGTGCTCTCATCCAGACGC GACTGTTTCGTCGGCACCTCAACGTGCAGGAGCAAGCGGGTCCTGTGCTACAGTTGCAGGAAACGGCGGAGGCGGCGGCA TTTGCGGTACCGCCTGTCACTCAGTTTCGCGGCACCCTCGATCGTTCTCGGCTGGATTCTGTCTCCTCCAGCGACGAGTC CAACTCACAGGCTTCGACCTCGTCGAGGTCCAGTTTTCGACTGAAGCGTAAGTTCCTTCCGCAAGCCTTATATGATAACT TCCGAGCCTTCAATCGGCTGCTGACAGACTCCCGTGTGCTGAGCCAAGCGGACGCGCATCTTTGGGACTGGGATGTTATT ACCACCATACTGAAGTCGAATTTAATCCGCAAACTGGACTACACCCAAGGAAAGTTCCTGAAGCGACTCGTGGACTTCTA CAAGCCGCGCAATAATCGGTTCTCTCACCAGGATTTGGTGCCTGGACAGAGGCTGCCTACATATGTCAGTGCCGGATTGG ATTTAATTGACGTCCTCCTGTCTAGCAATGAGCTTGAATGTATGCGCTTTATAACCGACTACTTTTCGGACATCAGCCAA CAGCTGGCGGCCGTCACCACATCGAATCGTGCACACGACTGTCTATTTAGTCCACAGCATATGAATAACACGATGTGTCA GCAGTATTTCCTCTACATCGGACGCATGTGCCGAACCGTAAAAGGCATCGAAGTACTCAAAAATACCACCGTATTTGAAT ACTTGATCAATTTGGTGCGGGTGACGGACCACGTCTGCTATGTGAAGCTAATTGTGTCCGGCCTGAACTACAGCTACGAG AAGCTACCACGTCAGGTGCTGGAAAAGGCGCTTACGTCGGCGAAGACACGCGGACGTCTGTACTCCACCCAGTTCATGGC AGTGCTGCTGCGTGCCCGTCTGCCTCACTTTGAGGTATGGGGCATTCCATTGATTATCAACCAGACGCGGGATTCGGACC GTAGCGTGGGGCTGGCAGCCATGGATGTACTGGAGGAGGCCTGCCACGACAAGTACTACCTGGAGGAGATTGTCAGTCGG TGGCCCAATCTAACGCATCGTGGTGATGCAGGACGGCTGCTGATGGCCCGCTATTATTCACTGCCCCGCGGCCTGAACAG TACGATGGCACGAATCGAGGATGAGATCCGCTACTGGCGGAATGGGTACAACAAGCGCTACGTGCTCCTCGTGGAAGCCG ACACCCACTCAAGCCTGACGCTGCACATCCGCAATGAGGATGGCTACTACTCGCGTCGCAATTGCAACCAGAGACCACAG ACTGTACCACCAAACGTTGCACCTCATCTGTACGGGCAAATGGCACAGACTGGCCAGGGTATGACAGCTCTGCGGAAGCA CGGAGACCTGCCGCAGCTATTGGAACTGCTCAGACGCGCCAAATGCACGGACGATGCCGAGTGCCTTGAACTGAAGGCCG CCATTTGGGCACTGGCCCACGCCTCAACCCACTCCAATGGAATTGAATACTTTGTCGAACTGAATGCCAGACTATACGAG AAGCTTATTGTTCTGGTGACCAAATGCGAGGTATATTCGGTGAGGGCCACCTGTTTTAGTGCCTTAGGTCTTATCGCAGG CACACAGGCAGGTGCCAATATCCTTTTTAAGTTGAACTGGCTGAGCGTGCGACATGACAAGAACACAATGTGGCCCGTGC ATCAGCCGGAGGATTGGATGTCCAGCCAGTACACACCGGTGCGACATTACTACGAGGACGTGCCGGCGGACAATATGACC ATGATGGATGACTATATAGAAAGATTCTACGAAACGGGCTCAGACTTTTGGCACATGCTCGCCGATCAGACTGATGCGAG TGGTGGTGGTGGGGGTGTAGCCGGCACAGTCGTCGGTATGCATCCTATAGTGCAGGATCCAAATGCAACGATTACGACCA CGGACAGTGTTTGCACCACGACCGATGATGTGCACCCCAGAATGTTGGTTGGAGTGGCTGCCAAATCAAAGACTTTGCCA GAAGGTAGCAATCTGCGTCAGGGCAAGCACCAACGCTCGCTCTCCGAATCGAAGACCACGGATGTGATCAGCTTGTTGGG CAGTGGTGTAGGTACGATGTCGGGAGCTGTTCTCTACCCAACGCCGTATCAGCATCGAATACGCTACAACTCGTGCACGG ATTCAAACACTTCCGGCGTTAGCAGCTGCGAATCGGTGACCGGACGCACTGCAGCTGCATATGCCGCCGCAGCCAATGAG TTACAGCAATTCCCTTTGAGTCCTATTCCAAGCATGTCCAATCTGCTCGAGAGCGATGAGCTGTTTCGTCGAAATCAGCT AGCCACCAGTATGCTGCCCTCCACCCTCAGTCCTATGGCGATGAAGGGCTATGTCCAGCTACGCTCCCTGCGCAAGCACA GCCGACCCGTATTCTCCGAATCTTCGGCGGAATTCTACGACTTTGCGGAAATACTGGACACACCAGAGGTTCAGATGCGC AAGCTCGACTGGACGCATTCCCACCGTCGGCTTAAGGTACGCAGCTTGGACCGCCAGCTAAGTGATGTTTATAGACGCCT CTCCGCGGACGAGTTGAATGTGCTTCTGCCCACGCTTAATGCTCCCAAGTTCCTGCCTCCTAATGATTTGAAAGGGCCAT GCTATGCGGGTATCTGCTTGCCTAAGAACGTTCTGGACCTGTTTCCGACGCGTAACCTCAGTCGAACGTACGTTTCGCGG GATATCCAGGACCAAGATATAGTGGGCATAAATCTGCTGAACACCATGCTACGGCCACAGTGTCTCAATGACTCACTCAC CAATGAGGGCGGCGACGAGTCATCGGTTATATCATCGCTGTCGGACGTCTCGTCTGCAAGTCGGCGACAAACACGACAAT TGCAGCAAGGCGCCAAGCACTCGCGAAGTCTGTGTCTCCATTGTGCCCGCTCCCCGCGACAGCAGCGTAATGACAGCGGC AGCCATAGTAATGGTGGAGGAGGCGCATCCCTCGCTCCCTGCGAGCTGTACAGCAGTGCAGCAGCAGCTCTGGTGGCAGC CGGCATCCAGGCTGGACCAGTTTTGGCTAAAAAGAGTGGCTCATCTGCCCAAGGAGCTTCAGCGGCACTTGGCGCCGACA TCAGCTTCCATTCCCCAGAGAGCATGCTGAGCGAGGATAGCCTGCCGGACAGGCTTACGGCCTCCATTCTGTACAACGTG CAGCGTCTGGCCAATCCCGTTAGTGCCAAGCAGTCGAAAATGGCTCTGTTGGAGCTTAAGCAGAAACACCCGCACGCCTT TCAGGATATTTGCCTTTATTCGGAGGCCTGCAAAACGATAGGCCGGAGTAGCTATCGGATGATAGCCCGCCGCTTCCTGC AGGAGCTCTTTCTGGACCTTAATTTTGACTCGTTCTACGTGGAGCCTCAGCTCATAATCGGGGCACGTAAGTTTTCCGCT GAGGAAAAAGCAGAGGCCACGGCCCCCACGGCAATGCAGGCCATGCCGTTTAAGCAAACGATTCTCAAGCCTAAACCGGC GGCCCAACTGGCTAGCGTTTATGAGACTAGTTGGGAGAACCTGCTGATGGACCAGTCGCCACGGATAAACAAGTCATCGC CTGCCGATCAACCGGCCAATGCTCAAACGAAAGCAAAGGCCCGGAACCAGCTGCACATTGACGATACGGAGAGTTCGTTG TCCAGTACCGATGGCGAAAATGATTCCGGTGAGGATGTTTGCGACGGCAGTGGAGCCGGCACCCAGGCCAGCTCGGTGAC CACCGCTCATACAGCCCTGCCCACAGGAAACAGCAATCGTAGCAGCATTGGCACATGCACAGCAAGCCAGCTGGTGCCAG GTGCGGCAACTTCAAACTCCGCTGGTGCTGGGACAAGCGCATCTGATAATGCTAGTACAAGTCTAAAGAATGAAAACCGG CAATTCACACGCGGACGATTCTATACGCTGGAGCTCGATTTGAGCTGCACCAAGAACAAGTTTCCCATCAAGAATCGCAG CCAGCGAACACCAACGCCTCCACCAATCGAAAGTTTAATCGAGTCCAAGCCCACGGTTCAGACAGATGTGAATACAAGTG CGCTGCCAATTATGCAGCGGACCGGTGGCAGCAATGACTCCTCGTCAGCCCTAAGATATTCCACTGTGACCAAGAGCTTG TCGGCATCGATGGCCAGGCCGGTGGGCAGCCTGTACTGCGAGAAGCGCTTGCAGAGCTCCAAATCGGAAGCGGTCCTCGA TGAGGTGGCGAAGGCGAGAAGAGCATCCGGTGGTGTGGGACTGACAACTGTGCGTGACAACTCCTAGACGCACTGGCGGA AGATCAATTGAAGTAGCCCAATGGGGACGCACTGCCTACTCTTACATCTCGTTTACTGGCCGCCCGCCTTCCTAAACCGA AACCAATCGGATAAATGTAATATATCAAATCAAAGAACATGTAGCTACGTAGATTAAGGCAATCCGTACTATAGGATTTA AGTTATTTTCGACTAGTTTGTTAAAATTAAAGTTATTACCATAGTTACTTTTACGATCACCACTTATGCATAGTTATTTT TAAAGAAATTCATAACCGTAAGCAGGAAACCAACAAACGACAAAAATCCCAAAAAATAATATATAATTTTCTAATGTAGC GTGTACCAACATATTTTATAGAAGCAGATCGAAAGCAAATTGGATAATTGCCTTGAAGCAACAAGAAACAACCACAAACC ACCGCAATTTATTTAATTAATGTACTTAAAACTAAAAGAGACCCAGTGGCAGGCGAAAGCAAAAAGACCACACAAACCTT AAACCAAGAAATTATCATTATTGTTGAAAACAAAGCAAGAAGCATACCGTGAATCGTTCACAATCACTTTAAAACCGAAA TGAAATGAAATCAATAAACTATAAAAATTGAAAAAGTTAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from cultured cells; cell line used was not derived the iso-1 sequenced strain.
For the SD cDNA library, Schneider L2 cells were collected.
RNA was poly(A)+ selected once. oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~300,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).