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FB2026_01
,
released March 12, 2026
ATTTATTTAGATGTTTTTTAATTACAAATTGAATAGTGCTTAAACCAGGTAGCGCAACCAGTTGAAAAAGAGCGCAGCAG GCACTCGAAATGTTCGCCAACCTGAAGAACAAGCTGATCGAGGAGGTGAAGGCGTCGCCCTCGAAGTTCCAGCAATTTGC GAATGCTGCACAGGCCGCCGTGAGCTCATCGTCGAGCACAACACCCAATTCGGAAACGAACACCAGCAACAATGAGAACT TCTTCAGCATCACGGAGGAGGACACACCTCAGAACTCGCCTTATCGCATCCAAAAGCTGCCGGCCACAAGTGCATCCGCT CTGCGCGGACGATCGACTCAATCCCTGAACGGCGCCACCTCCAGGACCCGCAAGCTCTCCAACTCGTCCATGGCCAGCGA CGTGTCTTTCCGCCTGCCTACATATGAAGCGCCAGCTGTGTACCACTTGCAATCTGATCTGGACGAAACGAGCAGCGAGT TCGACGACTCCGCCTCAACGGCTCGTCTGGATGTGATCACCAAGGACCAGCTGTACGATGCGTACAAGAAGTCACTTGAT CGTTACCACAAGTACCGCTGTCGGTACACGGATCTGGCCAAAAAGTACAAGGAACTGGAACGCGACAGCTCCAAGGCGCG GTCTGTGCTGGTGGAGACGCAAGACAAGGCGCTGCGCCGAATCAGCGAGCTGAGGGAGCAGTGCACGCTGGAGCAGCAGG CCAAGGCGCATTTGGAGGAGGCTCTGCGCGTTGAGATGGACGACATGAGCTGCAAAATGCAGGCTTATCAGACGAAGCTC CAGCTGCTGGGCGAAAATCCTGAGAATATTACGGCGGCTCTGGAGAGAAGCGGCCAACAGCTGGAGTCCGAACAGCTAAT CGACCTGGAGGAGTCGATCGGAAAGTCGCCCCTGTCCACCAATGGCAGTTCCGGTGTGTCCGACTTACAGAGATTACTTA AAGAGCGGGACGAGCAGCTGAAAAGTGTGACCGAAAAATACGAAGCTGTGCGCAAGCAGGAGGAGGAGAACGTGCTTCTT CTGGCCCAGACAAAGCAGGCCATCCATACGGAATTGGAGCTAAAGGACACCGAGGTGCGTAAGCTTCAGGAGAAACTCAA GCAATTGGAATCTCAGCGAGAGTCCCACAACAACGAAGTCAAGGAGCAGTTCAAAAAGCTTCAGGCCACGAAGCAGGAAG TCGATGCCAAACTTATGGCTACAGAGCACCTGCTGAACACCCTAAAAGAAAGCTATGCGATCAAGGAGCAACAGGTGGTC ACACTCGAAGCGCAACTGGAAGCCATAAGGGTGGAAAACGAACAGAAAGTAAAAGATCTGCAGAAACAGAACGAAGATCG AAATACCCAAGCAAGCGATTCAAGCGAGCAACTAAAGAAACTTCAAGCAGCCGTTCAGGATGCGGAGTCCCAGCTTTTGT CCAAGGATCAGCTCTTGGAATCTCTCCGAAGCGAACAAGCAGCCAAGGAGCAGCAGCTAAAGCATCTGAAGGAGCAGTTG GGTAAACTGAAACAAGAAAACGAAAATTACTTAGATAAATTGCGAGAAAGCAAGAAAAGTAGCGATTCTCAAACTAACGA AGCACAGGATCAGCAAAAGAAACTGCAAGCAGCCAAGGACGAAGCCGAATCGAAGCTCTTAGCCACGGAAGAACTTTTGC ACTCCCTCAGGAATGATTATAAAGCACAGGAAGAGAAAGTCGCTTTGTTGGAAGACAAACTGAAAACTCTTTCCAAGGAA AACGATGTGAATGTAGAAAAATTGCACCACATTAATGAACAACGAGAAGCTCAAAGTACTGATTCCCAGCAGAAGATCAA TGAACTACGTGCAGCTAAGGACGAAGCCGAAGCCAAACTTCTTTCCACTGAACACAGTTTGAACGCACTTCAGGCCGCAC TCTCGGCCAAGGAAGAGCAAGCGGCATCCTTGGAGCAAAGCTTGAATGCGCTTAAAACCGAAAGTGAGCACAGTTTGCAG GACCTGCGCTTGCACAATGATCAGTTGCTCGAAATTGTCCAGCGTCATCAACAAAACGACTGGGAAGCTCAGCTCGCTCG GGCAAGAGAGGAATTGGCAGCCATTCAATCGCAACGGGAGCTCCATGCTCTCGAACTCGAAAAGAGCCTGGAAATGGAGC GCGAATCTGTGGCCGCTTTGAATTCCGAGAAAGCCTCCCAGGAGGAACAGCATAGGCTTAAGCTGGAACAGCTACAGCGA GAGATTCAAATACTACAAGATCAGCACGCGAACTCGGAGAGCGAAACAGTGGCTGCACTAAAAGGGCAGTTGGAGGCTCT TAGTCAGGACTTGGCCACCAGTCAGGCCAGTTTGTTGGCCAAGGAAAAGGAACTGAAAGCCAGCGGCAACAAGTTGAACA AGATAAAGAAGCAACACGAGCAGCACCAGGCGAAATCAAGCGAACAAAGCGTTCGCCTCGAAGCTCTGCAAAGTCAGTTG GCTGATCGACTGAGTCACTCTCGCCAGGTGGAGAGCGAAAAGGAGGAGCTGCAGGCACGCGTCACTGGAATTCTGGAGGA GATTGGCACCATGCAGGCGCAAATGCAACAGGTGCAAGACTCACACAGCGAGCTGGAACGGGAGAAGCGCAAGCTGGAAT CCCGCATCGAGTCACTGCAGCAGGAGCAAGTGGACAGCAGTGCCCAGGACGAGAGGACCTCAGCCAAGCTGGAGGAGATA CAGAGCGAAAACACCAAGCTGGCCGAACGCAACTGTCTGCTGGAGGAGCAGGCGAATCATTTGGAGTCCCAACTGCAAGC CAAACAAGACGAGATCGGCAAGATCCAGGCGAAGCTGCAACAGGTACTCGACGAGCACTCGAAGCTGCAAAATGCCCAGG AGCTGATGGATCACGACCATCGGACGCTGCAGGATAAATGCGATGCCTACGAAAAGGATAAGCTGCTGACCAAACACACT CTGGACTGCCTGCAGTCGGCCAGTGAGGAGCTGCATAGGGTTAAGGCTAACTTAGATCGAGAACTGAAGGAGCAGGACCA ACAACTGTCGGAGCTGAGAGAGCGACAAAGAGAACAGGAGCAGCAGCTGAAAGACCAAGCCGAACGATGCGCGAAATTGA AGGCCCAAAACAGCGAAAGTGAGACCCAACTGCAGGCCACGATCAGCAATCTTCGCGAGCAACTTGATGCTTATAAGCAG ACAGAGCAGGGCATTCAGGAGAAACTGCAGGCTACCAATTCGTCCTACACCACGCAAATTGCCACATTGGAAGCTCGCTG GAGCGCCGCCAACTCAGACGTAGAGCGTCTTCACGAAGCCAATGATGCCCTGCAGCTGGAGATGGAACAGTTGAAGATTA AGCACGGCCAGGAACGCGAGGAGGTGAAGGAGTCAATTGCCCAGAAAAACCGTCAGGTGGTGGAGCTCCAAGAAGCCATG GCCACAAGGGATCGTCAGCTACAGGAAAAGATTGAAGCCTCCGAAAAATTGGCCAAATTCGATGAGATCCTGATCGAGAA CGAGTATTTGAATAAGCACACGAAGCAGTTGGAGGCCGAGTTGGCTGAGAGTGCGGAGCTGAAGGAGAAACTGAAATCCC TGCAATGTGAATTGTACGTTCTTCAGGAGAAGGCCGAGCAGCATGCCGTCCAAATGGCCGAAAAGGAAACGCAGAGTGCG ACAGCAACGGCAGAGGTGTCCGAACTGAAGAAGGCCATCGAGGAACAGGCGGTGGAGCTGACCCGCCAGAAGGAGCACGC CAGCTTTGTCACCGAGCAGAGTGATGCGGTCCAAAAAGACCTTCTTCAAGCGCAGCAGCAACTCCACGATAAGCAAATCG AATTGGCAATGAGTCGGGATGAGCAGGCCTTGCTGCAGGCTGAAGCTGATGGCTTAAGGCAGGAGATGATTTGCCTTAAG GAGCATTTATCCCCCTCAACCGACTCCGACAGCCTGCGCAGCCTGAACGAGCGACTGCAACGGGAGCTGGAGGATCTCAA GCACAAGAGCGCCGGTGCGGAGTCCAATATGCAGCAGGAGATCGAGGAGCTGCAGGCTAACAACCAGCAGATGGCGGAGC GCATCAACGAGCTGGAAACCCTGCGAGCCGGCATACAAGCCCAGCAACTCCTCGCCAGCATGGCGCCCAAAAACGTTCAG GAGGCAGCCGCTGCCGGCGAGAAGGCTGAACTGGAGTCTAAGCTCAAAGAGATTATGAACGAGGTGCAGGACGTGACGAA TCGAAACCTGTTCCTGGAGCAAAAATGCGAGAACTTCCTCATACTCGAACAGTCCAACGAGCGACTTAAGCTGCAGAATG CGAAACTCTCGCGGCAACTGGATGAAACCCTGGTATCCATGCAACACAGCGAGGCTGTTCCGGCCAACACGGAATTCGAG TACTTGCGAAACATCATGTTCCAGTACCTAACCGGGAACACGAACAACGAGACGCTTGTCAAGGTGATATCGGCAGTGCT CAAATTTTCGCCGCAGCAGGCCCAAGTTGCACTGGAAAAGGAGCATCAGCGGCGATCGCTGCTGAACAAACTGATCTAGC ATGAACTGATGGCTCTCTACGGCGGAGATTGGAACTGGGAGCCCTGCCCGGTGGCGCTTCTAGACCGCAAATGATGTCGG AGATCACAAGTACCTTATACTTATACCTCACCCTCGCGCACGGCATGGGTGCTTCCGGCTGACTCTGCAGGCATTTCGAG TAGTAGAAGCCGCTGATGTGTGTGTATGCCGCATCCTAGATCCATGTTATTGTACAGTCCACGAAATAAGTATAGCTACT TCTGTCCAAATCCACGCTAAGCACTTTTGTACATTTTGATTACGCTTTCCTTTGTTTCATTCACTTGCTTGCGCTCCATG TCCCATGTCCACGACTTACCTTAACCCCAGCCTAACCTAAGTGCAATCGTTTTGTGTATTTTAGTTGTAAGTTTAAATTT GTAAAGATTCGCAGCGCAGTTGGCATTTAGTTTAATTCAAAATCTATACAATAACATACATAAATAAATAGTTACAATTC ATAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from cultured cells; cell line used was not derived the iso-1 sequenced strain.
For the SD cDNA library, Schneider L2 cells were collected.
RNA was poly(A)+ selected once. oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~300,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).