FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\AT19506
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General Information
Symbol
Dmel\AT19506
Species
D. melanogaster
Name
AT19506
FlyBase ID
FBcl0295558
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG14322
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    AT_cDNA
    • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      seminal vesicle
      (Stapleton et al., 2002)
      testis
      (Stapleton et al., 2002)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      4,659
      NCBI
      AY089375
      Full Length sequence dataSequence Downloader
      AGGCAAACGCCTTACCCGAAATCAAACTACGAAAATCGGGGCCGTTCTGACACCCGGAGTTCAAACCAGGAACGACAGGA
GAGAGATTATACCAAGAGAACTGATTTTCGTGAAAGAAACACTAGATTCTCCGATGAGAGACACTCTGGCAGATATAGTG
ATTATCACCGTAGAAACCATTACCACAGATTTAAGTCATGGGGAAGTGAGGGCCGATCCTATCGTAGGGACAAAGGTGCC
AGAGATTTATCCAAGTCACGTTATGATAATGATGCAAGTGGTTCAAGTCTAATCAGAGGTGCATCTGAGATCAGGCGAAA
AAACGGACACAACTGTGATCCGAATAGCGATGCTTCCAAAACTGAGAATTCTGAGGATATCCAAAGTTGTATAAATCACT
ATCAAACCGAAGAAAATAAAATTGATACAGAGCAATCTAAGGATCAAGGAGCTAACAGAACCAGCCCCACCGAGCAATTA
TCAGATATCTCAAAACAATCTAATCCAATTGCATTAGAAGGCGATCAGAACAAAAAGACGAATGAGTTGAAGGAGAGCTC
TTGCTCTAGCAAGCCAAGCAGGGAAATAAATGAAACTTCTAAGGCTTCACAGTTTCCCGATCAAAGATCGAAGGAACAGT
CGTCGGGGGAAGAACTAAACGTATCCGAGTCCAGTGATAATCACTCTGGTGCACAGGCTAGTCAATCAATTACACGTATC
CAAGTGCGCCCCCTTACCAAACTTCTAAGGCAGGAGCTCTTTGCAGTCACCCAGGAGAATCGTCTGATCAAAAGTCCCCC
TCCATCCGCATCCCCAGCCAGTCCCAGTCGTCTGGTCTTTACGCCCAACCTTAGAAATCGTCGCCGAACAGTGAGCAACT
GCATCTACAATGCTGGAAGTTTTGATCAGACCGCCGAGAGCGAGGCCGTTTTCAATGATCGCATCGCTAGCATGGACAAG
GAGAGTCTAAAGTACATCATCAACAATGGCGACACCATCTTTGAACCGCACTTGCAACTCCAGGCCAGGCGGCGTATACG
TGACGAAATTCGCCGACAGCTAAAGACAATTGAATTGGAGAAGCCCAAGGATTGTCTGGTAACGGATTTAGTCGAGGACG
AAATCGTAGATTCCATCAAATTGCCCGCATTTCTACTTGAGGAGATCGGAAAGTGTTTTGGCATAGACATATCAGAGGGT
CAGACGACCAAAGATTCTGCAGCAGAAAGAGAAGATACAACAAGGGTTAACGCGGAAAGCCCAGTTCAAGATACCGAAGA
AGAAACGAAGAGAGCTTGTAACGGCGTCGAAAGTTTAAAAGATATAAAAACTGAGGCGACTGCTGATGATAGCAATCAGT
GCACTAAATTTAGAAATCTTGAAAACGAAATTGATATTAATGATAACCACCATCAATCAGAAGACGACGAGCTTAGCAAA
CCGCTCAGTGTATTGCCTAAAGATAGTGAAGATTGTTCTGTAAAATCGAATGGGGTTAGTAAAAAGGTCTTGCAAGAAAA
CAATAAGAAGAAAACTTTTAATCGAGACACTAAAGTAAACGGAAATAAGGCAATGAATCCGCCACAAGAAACCGTGATAG
AAACTCTGGAACACTCTGATTCCATTAACAGGTCCATAACTAATCCGAAAACAGCCGATGAGCTCAAAAGTTTCGCGGGA
ATTGAATGGGAAGTGAATTCGAAGAGTAAGCACTCCAACCCAAGTCAATCCGCATTCATTAAAACCAACAAAAGTAATAA
TATAGAAACTGCTTTAATAGATTCCCTTTCTGGGATAAGTAAGCCCTGTAAAATGGAAATTAAAAGGGAATCAAGCAGCT
CACCCATATCAGTAAGTTCTTTACCTTCAAACGATGTTATTGATCTGCTGAGCTCATCTGACGAAGAGCTGGAAGAGCAT
GCCGTGGTGGATATGGACATTGATGAACATGAAGGTGATATTGAAAATCAAAAGATATTTAAGACCTTGGAAGCTTTGAA
GCAATCAGATGATGTTGTTGATAGTGATGACTCCACCAGCTCCCAAAGCAGCAGTAAATCCACAAAAAGGCGACGCAGGC
TGAAGCTACAGAACGATGCAGAAAATGTGGTTGACAGTTTTGAAAAATTGATCCTTCCCCATCTTAGAGAGGCTCTCAGC
GATCGCTATCGTCGCCAGCATTCTAGTAGCCTGCAGAGTCGATTGCACTTTATATCCTGCGTAGTGACAAGCTCCGAGCA
CAATGCACAAACCTTTAGTAAAATTGAGGTAGCCAAGATGCAAATGAATCTCAAAGCAGCCGACAATCGCCAAGCTATCG
AATTTCTTCTAAAAGAGATTGTCAACGTGGTGAGTCTGCAGAAACAGCGTCGTCGGGAGCAGGACGAAGAGCAAAAGCTT
GCAAATTTATTAAGCCCCAAAAAAACCGCATTCACATCTGATGAAACCTGTAAGGCGTCAACACTATGTCCAAACCAGCA
CGGTTTTATTCCACCAACGGCTCAGGATAGTTCGCCAGTTACATCACCGTGTCAGAAACCTCCATCTTCTCCGCCATCTC
AGCAGAGTCCTTCTTCCGCAACTGCTCAGCAGAGTTCTCCAATGGCTCTTCAGAAAACTCAATCTTGTCCAGCCCGCCAA
TCTTCAACGCCCATCCAGGAGAATCCTATGGACTCATCTGGACAGTGGCGGGGTTCAGCTAGTCAGAAAATTCAATCTTC
TCCTGCCCGCCAAACTACACCGGTCGAAGATGTTTTTGAAAACCCATCACCTAACCAAGGAAACCCTCCCTCTTCGGCGA
AACTGGAATCCTTCAACGTTGGGTTTCCATTTCTATCCATGGATCCTACACTATATAATTATTCTCGTTTAGCTGGTGGA
TCAAAAATAAACGAACCTTTGGGAAATTCGGACGACATGATGGAGCAGCATTTGGTTGAAATTGAGCGTGAATTGATAAA
ACATGAAAATCGTTACAGCTTTCTGGACGATATAATAATAAAGTTTCAAAAGGAGAAATCTGAAGTCGGTATGGTTATTC
TAGAACTAAAGAGTCGGAAGTTTCTAATTATTAACTCCATGGCTAGTAGAAATCAAGCTACTTCTGCTCAAGTGGCTGAC
TTCAAAAGCAAACCACATGAAGCAGAAACTGCTCAGGAATCAACACACGAGGATAGTTTCAGCAAAGGAGGGATAGCCAG
ACGAACTAGAAGTAGATTAAGAAGAACTGTGTTGGTCCTGGCACCCAAGCGTCAAGTAAGGGTACAAAAACGAGTTTCCA
AAAAACCAAAATCTTTCAAATTAGTGGAGAAATCCCATGAAGAGATTGCTGAACAAGAGACGAATAAAATGGATTCTGAA
GCGACCAATCAACTAACTATCAATGAAGAGCTGTTGAAAGTGTCAGCTGGTGACCAAGTAGCCGATTATTCGCCCACCGC
CAAACTTGTACAATCGCGACTGAGCAAGCCCAGTATTGCTGTTAATCCAACCCATCAACCACTGGCTATAATACCACCAC
TACCACCGCCACCACCACCTCCCGAACCAATCTGCCACATGTCCTATGAAGCATCCAGCTCTTTTCTTAAGGAACCACTA
CACGAACCGGGTCACCAATGGTCAGAGCTCAACTCCGAAGACAAGACAAGACAAGGTTTTGTGACGAAGGGGAAATTGCA
AAATGTTGGCAGCCCCATTACCCAAATAAAGATCTACAGGGATAACGTGATAGCCGCAGCCGAGGATGGAGATATCTACG
TGTTTCACCTAGTCACTCACAAGCTGGAGCAAAAGATCACCAAGCACAGTGAGGCTATTACGAATATGTTCCTCAGCGAA
AAGGATTCTATTCTTTACACCACATCAGCGGATGGCTTTTTTAAGAAGTCCTCGCTCTTGAATCTGGAGCGGGTCTTTGA
AACGGTGTACCTTAAAGAGCCATTGCAGTCAATGGACGTCGCTTGGGGATTGGCCTTCATTGGCAGCCGATGGGGTCAAA
TATCTACCTTCAACGTGGTGACGAACAAGGTGGTGGAAAAGCCTTTGGTATCAACCGGCCAATCCATCATTGCGATCAAG
GCTACCAAGGAAGGTGTGCGGAAAATCCTTGTGCTGGGATGCAAAGGAAACTTTGTCCAAATGCATGATGCGGGCAACGG
ATTACTTCTACGACACGTATTTATTGCAGAGGGTTTAAATATCTACAGTCTGCTTCTAGATGAAGGACACATTTATTGCG
GAACGCAGAAAAACGAGTTATATCAATTGGAGTTTGTTTCTGGAAACTTGGTCACCAAGTTCAGCTGTGGAAATGGCGCT
GTTGCTGTTGCGGCCTACGGTGAGCGTTATTTGCTAGTCGGCTGCTACGACGGCTATATTTACGTCCTGAACAAGATTAC
CGGAACTCAAACTGGTCGTTTCGCTGGCGCTGGTCGCATGGTTTTAGCCCTTTCGGTTGTAGGCGATAAGATCGTCACAT
CTTCTAAAGATAACTCGCTGGCGATTCTGGAGGTGCCCCCCGCATTGGTAAATGGCTATTAAGACGCGTATCATCATTGA
TATAAATCTGCTTTAATTTAATCATTTTTACACACAGACTTGTTTGCATAATACAATTTAATGGTCATAGTCAAAAAAAA
AAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: AT_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

      (Stapleton et al., 2002)
      Sample preparation

      Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

      Protocol

      RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

      (Stapleton et al., 2002)
      Comment
      Reported As
      Symbol Synonyms
      AT
      (Stapleton et al., 2002)
      AT_cDNA
      References (1)
      Research paper (1)
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)