FB2025_04 , released October 2, 2025
Clone: Dmel\IP12105
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General Information
Symbol
Dmel\IP12105
Species
D. melanogaster
Name
IP12105
FlyBase ID
FBcl0328440
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG42748
 
Collection Status
N/A
Known Problems
contains transposon sequences
FlyBase assessment
    Library
    IP_cDNA
    • IP cDNA library of D. melanogaster, isolated by an inverse-PCR screen (SLIP) from other cDNA libraries.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-22 hr AEL

      (Rubin et al., 2000)
      larval stage
      organism
      (Rubin et al., 2000)
      pupal stage
      organism

      Comment: early pupae

      (Rubin et al., 2000)
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      5' Sequence
      Total bases
      756
      NCBI
      EL882327
      5' sequence dataSequence Downloader
      AAACATTTAATCGTGCGGGGTTTGGCAATAGGCGCTTAGTGTGTGCTGCGCCTGACGCAAGTGTATTTGCGTGTCGGTGT
TGTGATTCTCGCGCCATGTTTGACCAACCCCCTGAAAACGAACTGCAGAAATCACATGACCACAATCCAGGCGCACTAAG
CGAGCATCAACTGCGTGTCCAAGCTTCGCTTCAGCGGCTAAATATTCCCGACTGGTTCCGTCATTACAACAAAGGCCCCG
AAAAGGCAGCAGATGGCGAAAACGCATGCACAAGTGCGACTGGCGGTGGTTACCGACCTGGCAACTTCACCCGCAAACGC
GCACAGGAATCGGGTCGCTGGCAAGGGCTCAACTCAAAGACGACTTCCCTCAGCTCGCTGGGATCTCAACGCTCCGACCG
AAGTCCTTTGTTGATGAGTCCCTCAGCGCACAGTCATCACGGCGGCCAAAGCTCCTATTCATGTGGGTCGACGACAATTC
ACGGTCCGGCACCTGTACAGATAGGCATGGGTGCAACTCGCTGGTCAACCTCTCATCTGAATTCCACTCAGATGTCTCCC
GGTGCCTCGCAGCGCGGAAGCTTTACGCGCGGCGCCCCCATTAATAGCAGCTTTATATCTGTGGCCAGTGGTAGTGGCTT
GTTGCGCAATTCCTACCGACAGCCGTATTTGGGTTGGCGCAGCACAGAGAAGCTCTCCCAACGAACTCCCCCACGAACGT
CTGGGCCATTTCGCTGCTAACCCAACGGACAGCTCC
      3' Sequence
      Total bases
      550
      NCBI
      EL882326
      3' sequence dataSequence Downloader
      GAAAACATAAATATTTTAGATTAACTCGTTCCTCAAGCTTAAATTTTATGGGGGAGGGACGCCATTAAACAAGTAAGTAA
TTATACGATTTTATAGTAACATTTTCATTTAAATGACTATGCTATTGGCTTATATTTGTCATTTTGGGGAACCGATCTTG
GCTATTTAGTATTTAGTTTTTAGTTAAACAACAATTTCATTTTTCGACTGCCTACCATGCCAACCGTCCTTTGGAAGTGG
GTTACAGCTTCCCACTCCACTCACCCACTAAATTCAAAAAAATTAAAAGTCCTGGCCTAAGCCCTATCCTGCTGTTATGC
AATATTGGACAATAATCAAGTTAACCAAACCATTCGAGTGCTACTTAATGTATTGCAGTATGTAACCGAAATCTCCTGTC
CAGAATGGCGGTTTTTATGCGATTAAAAACAATAACAAAAAAGCAATTTCAACAGTTACCTACATAAATGATGACTTCTC
GGCATAAATAATTTGGGCTTCATAACTTAAAATACTTTTATTACAAATAATTTAGGTAATTTTCTTAAGC
      Library Information (1)
      Library: IP_cDNA
      Description

      Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.

      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      These clones have been isolated a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.

      (FlyBase Curators, 2016-)
      Comment
      Reported As
      Symbol Synonyms
      IP
      IP_cDNA
      References (1)
      FlyBase analysis (1)
      FlyBase Curators, 2016-, Curation of large dataset metadata by FlyBase.
      Curation of large dataset metadata by FlyBase. [FBrf0232540]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)