FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\IP03463
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General Information
Symbol
Dmel\IP03463
Species
D. melanogaster
Name
IP03463
FlyBase ID
FBcl0328993
Feature type
cDNA_clone
Computed gene(s)
Dmel\Eno
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    IP_cDNA
    • IP cDNA library of D. melanogaster, isolated by an inverse-PCR screen (SLIP) from other cDNA libraries.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-22 hr AEL

      (Rubin et al., 2000)
      larval stage
      organism
      (Rubin et al., 2000)
      pupal stage
      organism

      Comment: early pupae

      (Rubin et al., 2000)
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      3' Sequence
      Total bases
      836
      NCBI
      EL871350
      3' sequence dataSequence Downloader
      ATAAATAATGATTAGATCAAGGTTTAGCATTAATTAATAATGTCTAAGAACGAAGCATAGAGACAGCAAATGGGTTTGGT
AGTTTAATTCTGATGTATTTGTGCGCCAGACACAAGTTCCGAACGTAAAAGGTTGACGAACACGTGCACCGCCGCCTTTC
CATGGATCTACTGCGTCCAGCTCCCTCTCCAAGATCCAGATCTCATTCGATGGGGCTGGTGTGGTTGGAATTTCGAAGTG
CCCTGCGCTTACTGCGGCTTCCTGAACGACTTGCCGGCGAACTTGACGCCAGCGCCGATCTCCTCCTCGATGCGCAGGAT
CTGGTTGTACTTGGCCAGACGCTCCGAGCGGCATGGAGCTCCGGTCTTGATCTGGCCGGTGGACAGACCGACGACCAGGT
CACCGATGAACGAGTCCTCGGTCTCACCGGAACGGTGGGAGACCATGGTGCCCCAGCCGTTCTTCTTGGCCAGCAGATGG
GCGGCAATGGACTCGGTGACGGTGCCGATCTGGTTGACCTTCAGGAGCAGGCAGTTGCAGGCCTTCTTCTCCACGGCGGT
GGCAATACGCTTGGGGTTGGTCACGGTCAGATCATCGCCCACGATCTGGATGTCGGTGCAGCCGGTCAGGTTGGACCAGG
CCTCCCAGTGGTCCTGGTCAAAGGGATCCTCAATCGAGACGATGGGGAAGTCCTTAATAAACTCCTGGTACAGGTTGGCC
AGCTTGTCGGCGGGCAGCCACTGCGACTTGTCGCTCTTCTCGTTCTTGAAGTCCAGATCGTACTGGCCATCCTTGTAGAA
CTCGGAGGCGGCCACGTCCATGCCGATCTCGATCTT
      5' Sequence
      Total bases
      738
      NCBI
      EL871351
      5' sequence dataSequence Downloader
      CCAGCTCTCATCTGGATTTAAATTTGTGCAGATCCGCAAAAGTACTTGTGACAGCAACGAGATGACCATCAAAGCGATCA
AGGCCCGTCAAATCTACGACTCCCGTGGCAACCCCACCGTGGAGGTCGACCTGACCACCGAGCTGGGACTCTTCCGAGCC
GCCGTGCCCTCCGGCGCCTCCACTGGCGTCCACGAGGCCCTGGAGCTGCGCGACAACGACAAGGCCAACTACCATGGCAA
GTCGGTGCTGAAGGCCGTGGGCCATGTGAACGACACCCTGGGACCCGAGCTGATCAAGGCCAATCTGGATGTGGTTGACC
AGGCGTCCATTGACAACTTCATGATCAAGTTGGACGGCACCGAGAACAAGAGCAAGTTCGGAGCCAACGCCATCCTGGGC
GTTTCCCTGGCCGTGGCCAAGGCTGGAGCCGCCAAGAAGGGCGTGCCGCTGTACAAACACATTGCCGATCTGGCTGGCAA
CAAGGAGATCATCCTGCCGGTGCCCGCTTTCAACGTGATCAACGGCGGCAGCCACGCCGGCAACAAGCTGGCCATGCAGG
AGTTCATGATCCTGCCCACCGGCGCCACCAGCTTCACAGAGGCCATGAAGATGGGCTCCGAGGTGTACCACCACCTGAAG
AATGTGATCAAGGCCAANGTTCGGTCTGGACGCTGGTGATCNCGATCNCCANCGTCCAAANGCCATGAAAATGGGCTCCG
AGGTGTACCACCACCTGA
      Library Information (1)
      Library: IP_cDNA
      Description

      Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.

      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      These clones have been isolated a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.

      (FlyBase Curators, 2016-)
      Comment
      Reported As
      Symbol Synonyms
      IP
      IP_cDNA
      References (1)
      FlyBase analysis (1)
      FlyBase Curators, 2016-, Curation of large dataset metadata by FlyBase.
      Curation of large dataset metadata by FlyBase. [FBrf0232540]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)