FB2026_02 , released June 18, 2026
Clone: Dmel\FGC020G10
Some tools are temporarily unavailable. We are completing maintenance and working to restore these tools as soon as possible. Thank you for your patience.
Open Close
General Information
Symbol
Dmel\FGC020G10
Species
D. melanogaster
Name
FGC020G10
FlyBase ID
FBcl0385643
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG3663
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    EG_pDNR_cDNA
    • EG cDNA library of D. melanogaster, embryonic gonadal cells purified by virture of EGFP-vas expression, pDNR-LIB vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 13 -- 16
      gonad
      (Shigenobu et al., 2006)
      Sequence Data of the Insert
      5' Sequence
      Total bases
      553
      NCBI
      BP543501
      5' sequence dataSequence Downloader
      ACAATTTGCAGTTTATTTAACAAGTGAACATTGCGTATTTTCTAAGCCGAATCTAGTAAATAGTGTGAAACAAAATGGCC
TNGTGATGCTGTCACTTGAACCCGAAGAAAACGCTTTTCCTGTTGTGCGACATTCAAGAAAAGTTCCGACCGGCCATGCC
GCTCTTTGATAACATGATCAAAAACGTCGACAAACTAACTCGGGCGGGAAAGGCTTTGGATGTTCCGCTGATTGTAACCG
AACACTATCCGGAAAAACTAGGAAAAACAGTGGCCCAACTGGATGTAAGCCATGCGAAGCTGGTCTCCGGTAAAACCCTT
TTCAGCATGTTTACTCCCGAAGTCAAGGCTGTGATCAAGGATATCTTCAATGACAAGCCAGAGGATGTCGTGCTATATGG
CCTCGAGTCCCACATTTGCGTGGAGCAGACCGCCATCGATCTCCTCGAGCAAAACATCAACGTATACATAGTGGGCGGAC
TGCTGCTCCTCCAGGCTGAACCAGGATCGAGATCTAGCCCTGGATCGCCTGCGTCAAGCGGGCTGTGTGATCA
      3' Sequence
      Total bases
      599
      NCBI
      BP543502
      3' sequence dataSequence Downloader
      GCTTGGCACTGCCCGCTGGCACTCCGCTCCCGTTGCGCGTATGTTCCATATCCACCGACGGCTGGTTCACCAATTTCCGG
ACCACATCGAACTTGGGGTTGTTCTTATCTTGAACCAAGTCGAAGATAACGCTCTCACTAGGGGGGATCACACAGCCCGC
TTGACGCAGGCGATCCAGGGCTAGATCTCGATCCTGGTTCAGCCTGGAGGAGCAGCAGTCCGCCACTATGTATACGTTGA
TGTTTTGCTCGAGGAGATCGATGGCGGTCTGCTCCACGCAAATGTGGGACTCGAGGCCATATAGCACGACATCCTCTGGC
TTGTCATTGAAGATATCCTTGATCACAGCCTTGACTTCGGGAGTAAACATGCTGAAAAGGGTTTTACCGGAGACCAGCTT
CGCATGGCTTACATCCAGTTGGGCCACTGTTTTTCCTAGTTTTTCCGGATAGGGTTCGGTTACAATCAGCGGAACATCCA
AAGCCTTTCCCGCCCGAGTTAATTTGCCGACGTTTTTGATCATGTTATCAAAGAGCGGCATGGCCGGTCGGAACTTTTCT
TGATGGCCCCCACCAGGAAAGCGTTTTTTTGGGTTAAGT
      Library Information (1)
      Library: EG_pDNR_cDNA
      Description
      Sample preparation

      Fly gonads were isolated from embryos carrying EGFP-vasa transgene that express GFP only in pole cells by using fluorescence-activated cell sorting (FACS).

      Protocol

      Total RNA was extracted from the isolated gonads at embryonic stages 13-16 by using RNeasy Mini kit (QIAGEN). Poly(A)+ RNA was purified Oligotex dT30 (TaKaRa) from the total RNA. cDNA was synthesized and amplified by Long-Distance PCR using SMART technology (Clontech) and directionally cloned into pDNR-LIB vector (Clontech). cDNA inserts are flanked by linker sequences 5'-GGCCATTACGGCCGGG-3' (5' linker: SMART IV Oligonucleotide, Clontech) and 5'-polyA-CATGTCGGCCGCCTCGGCC-3' (3' linker: CDSIII/3' PCR Primer, Clontech).

      (Shigenobu et al., 2006)
      Comment
      Reported As
      Symbol Synonyms
      EG
      (Shigenobu et al., 2006)
      EG_cDNA
      (Shigenobu et al., 2006)
      EG_pDNR_cDNA
      References (1)
      Research paper (1)
      Shigenobu et al., 2006, Proc. Natl. Acad. Sci. U.S.A. 103(37): 13728--13733
      Molecular characterization of embryonic gonads by gene expression profiling in Drosophila melanogaster. [FBrf0193273]
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)