FB2026_02 , released June 18, 2026
Clone: Dmel\MIP05777
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General Information
Symbol
Dmel\MIP05777
Species
D. melanogaster
Name
MIP05777
FlyBase ID
FBcl0701804
Feature type
cDNA_clone
Computed gene(s)
Dmel\Tsp3A
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    MIP_cDNA
    • cDNA library of D. melanogaster, isolated by an inverse-PCR screen (SLIP) from other cDNA libraries.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-22 hr AEL

      (Rubin et al., 2000)
      larval stage
      organism
      (Rubin et al., 2000)
      pupal stage
      organism

      Comment: early pupae

      (Rubin et al., 2000)
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      1,617
      NCBI
      BT060443
      Full Length sequence dataSequence Downloader
      TCCATTGTTTGTACACTTTACTTTTCGTGCAATATTACTACGCTAATAAAAGGAGATTAGATAAATAAAGAGTGATTATC
AAGCGAATGTGCAACTGCCACTGCCAATGCTAATTGGGTAAACGAGATAAACGGGCGACTGAAGCGGACGCAAAGTGAAA
TCGGTGGCAAAGCGAGTGGTTAAAGTTGAAGATACGGATACAGATAAACGGCGAGGATGAGCAACTACCGATATCAGGGT
GCTGGGCTCGGGGGCGTCGGCATGGCCGGGGGGCGTGGCTACAGCGGCATCGAGGTGCACGAGGTGATGCATCCGCATCA
CTTCACCTACGTGAGCCAGTGCGTCAAGTACATGATCTTCCTGCTGAACTTCGTGTTCTGGCTCTTTGGCGGCCTGCTCC
TGGGCATTGGCGTCTATGCGTTCAGGGACAAGTGGGAGGACGCGAACGGATCGGTGCGGCTGGAGAACTTCTACGACGTA
TTCCTCAATATCTCGCTGGTGATGATCCTGGCCGGCACGGTCATCTTTCTGGTCAGCTTCTCCGGCTGCGTGGGCGCACT
GCGCGAGAACACATTCCTACTGAAGTTCTACTCCATGTGCCTGCTGCTTTTCTTCCTGCTGGAGATGGCCATCGCCATCG
TCTGCTTCGTGTGCCCGCAGTACATGAACACCTTTTTGGAGAAGCAGTTCACGCACAAGATCATCCACTCGTACCGCGAC
GATCCCGACCTGCAGAACTTCATTGACTTTGCCCAGCAGGAGTTCAAGTGCTGTGGCCTCAGTAATTCGGGCTACCAGGA
CTGGAGCAAAAACGAGTACTTCAACTGCAGCTCGCCGTCGGTGGAGAAGTGCGGAGTGCCATACAGTTGTTGCATCAATG
CCACCGACATATCCTCCGGCCTGGTGAACATCATGTGCGGCTACGGCGTTCAAAACGCTCCCGTTCCGGAGGCCACGAAA
CTGATCTGGACCAGCGGCTGCATAGAAATCGTTCGAGTTTGGGCCGAGCACAATCTGTACGTGATTGCCGGCAATGCTCT
GGGCATTGCGCTCATCCAGCTGCTGGTCATCTACTTGGCCAAGACGCTGGAGGGCCAAATCGAGCTGCAGAAGTCGCGTT
GGCTGGCGTGAGATCCGGGACAGCCCAAGTTCTACGGGCCCTACAACCGCACCTGAACATACATACTCTCGGAATCAGTA
CTCATTTTTTTGTGCACCACTCACGTGCGGACCGGATTGCCAGTAATGAAAAGTCGAAGATCGTAATGGAAGATAAGCCC
AGTAGTAAGCGAATATTACTTATGTTGTAATCGACTGCCTGGATAGCTGTATATACTCGTACATGAAATTGTAGACCCAT
CTGAAAACCACTTAATTATTGTAATCTCAAGCCGACCAAACAACGCCATATGTACATTATAATAAGTTAGTGACTTACTC
GCTGAAATTCGATGCATTTATTTATATGTAATTCCTTCGAAAAAAAAGCAACAAACAGTGCGCACAAATCTTGAATATCT
ATTCATCCAGAACCATTTCTGGGTTTATTATTAATCTAATATAATGTAGATGTATAATAATAAAACACAAAGTAAATCAA
AAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: MIP_cDNA
      Description

      Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.

      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      These clones have been isolated using a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.

      (FlyBase Curators, 2016-)
      Comment
      Reported As
      Symbol Synonyms
      MIP
      MIP_cDNA
      References (1)
      FlyBase analysis (1)
      FlyBase Curators, 2016-, Curation of large dataset metadata by FlyBase.
      Curation of large dataset metadata by FlyBase. [FBrf0232540]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)