GAACGCTTCGGAGTGAAGATCGTGCTCGTCCGATCGGTGCGCGTGATAAATACACGGCGAACAAAATTGAGTCAAGAGTC AGACGGTCGCAAGACAATTGACAAGACACGAAAAATCATAATAAACACAAGTGGAAAAGCAACTGCTTGAGAGCAAAAAA GAAATTGTTTTTTACAAATCTCGCACGTAAATTAGCAAGCGTTCGCAAGAAACAAACCGCATACAATACTGAACACAAAA AAAAGGGTATTCAACAAAAAACCAAAAATATTTGGCTGCAAATGTGAAGAGACGAGAGACAGCGGATATACTACCGAGTG AAATTTGTGTCATAAGCAGAGCCAAAACAAAACCGAAAAGCCAAATTCAATAAAAGGCCATAAGTGACTGTGAAACTGTA AAGTGATGTGTGGTAATCGAAAAACAGCATAAAGAATGGCCATAGACCTCCTGATCCTGGCCCTGCTGCTCGTCTCGTTC CTGATCAACCTGCTGGCGCTGTGTGCCTTTTGGATAACGCCTGGCTTGAGGACCACCGCCAATCGCTTTACGATCAACCT GCTGGCCATCAATCTCATTGGTTGCTGCATCTTGGCCCCCACTTTGTTCCTGGGACTGCCGGGAAAGTCTGCGGAGGCAT CCACTTCCAACGCCGAGACCCTGGAGTTCTTCTCGAAGCCGGGAAACCATCAGGTGATACTTCGTCGCAATGGCCAGCTG GTGGAACAGGACGGCGTGGTGGTGCGAAGGAACATCAGCGAAAACGGCGATACGGTGGAGACCTTCTTCAAGTGCAATGC CACCTACTGTCGCGAGTTGACCATCGATGAGCGCGGAGATGGTGGCTTTGTCATCACGGAAACCGAGACACACGAAGAGA ATCTCTCGGCCTTTGAGAGCTTACCCACGGAGGCTCCCATTTTGCCGCCCGTCCAACTGAGATGTTGGTCCATAGACATG ACTGCTGCGCTGGGAGCTCTGGCAGTACTGCTCGTGGTGGGGGACACCTGGTGTGCCGTTACGGATCCCCTGCGCTACCA CAGTCGCATTTCGGGCGTGAAAACCTGGATCTTCATAGCACTCACCTGGGTGGTGGGCATACTCTTTGGAGCCCTTTCCG CCTTCCGAGTTTTGGACTTTGAGGCGGACGCCCTGTTCAGCAGACAACGCCGCCTGGCAGTCACGTACTTCAACATCAGC AGCACCAATAGTATTTTCGGAGTGGTGTACGCCAGTGTCTACTTCATCGTGATCATCCTCCTGCCCTTCGGCTTCGTGTG CGGAATGTACTGGAGGATATTCAGTGAGGCGCGCGGAAATGGGCTGCGCATGCGCCAGAATGGCTCTTCGCCGCTGCTGC AGAGCGCCTTGAATCTGACGGCTGGCCAGCAGGCGGCTCAAGCCAACCAGTTCTCCAATAGCTTGTGTGTTCATCGGCAT TCCATATCCTCGGCCAGTTCGCATGGCGGCAACAGCAGCTTGGGCTTGGGCGGTCTGCAAATGCAAATTGATCAGCGCCA ACAGCCGAGGAGTTCGCCCAGTTGTCTCCGTCGGGACTCGGCCGCGAAAGTACTTTTGCCCACCATCTCGGATGATGGTG GCTCAGATGCGGAAAGTGGAGCCGGAGTTCAACTGATGCCCGTACAGGAGCACTCACTGTCAGATAGAAATCAGAACATT ATGCTTACTCTGCAAACAGCCAGTGGTGAAATAAAGAGGAACTACTCCGCCAGACAGCTGCCCCTGTTGGGCACTTCTTC GCAGGATCTGAGGGAGACTAATCGTCTGCAGGGCATACGCCAGGTGCACAGTTCGCCCAATCTCCACAAGTACACGGAAC TGCGCCAGGACTCGCTCAGTGAGGAGTGTGGTTCTCCGCATCTTTTGGGTCACGCCCAGCGGCAGCAGCAGCAGCAATTG CACCTGCAACATCAGCAGCAACAGCATCATCACCACCAGCAACATCATCCGCACTTCAGTTCGCCGCGCCACCAGCAACA CGGCCACGCCCTCCAGATACCCGCCATTCATGCCTCCCCCAAGGCTCTGAGTTACATGAGCTCCTTGCGACACCGTTTGA GCAACGCCAGTTCGCTATTCAAATACCGTGAGGAGTCGAGAGCAGCCCGCATCAGCATCCTGGTGGTGGTGATGTTTGTG GTCTCGTACCTGCCCTTCGGACTGCTGGTACTCCTGCAATCGCGACTGTCGGCTGCTAACTTTGGCGGATCCTCCCAGCT GGCCATCTTCATGATCCTGCTGGCCAATCTCAGTTCGCCATTCATCTTCGCCTACAGGAACAAGAGGGTGAGGCGAGGAG TCAAGCGGCTGTTTGGTCTGGATTCGTCGTCGGGCCTGCAGCGCAACTGCAGCAGCAGTGTAAAGACCAATGGCACCGCA GGTCCTGCTGCAAGTGGTGCACAATTGCAGCGCAACAGCAGCAAGTTGTCGCAGTACAGCAGCAACAGCTGCAAGTATCT CACCCCCCAGAGTTCCCTGGTCAGTCAAGTGCCGGTGCATACGACCTTGACGTTAAGGCCCAACAGCAGCTGCAGCACCA TCATCAATTTCGGTGGCGCCAGGGGCTCAGCGGACTCCGATGAGCAGCCACCGGCAACACCACCACCTACTGTGGCAGTT GCTCCACCGCCCACACGACCGCAGCGCCCCAAGCTGCAGAGGGGCATCACCATCGTTGAGCACATAGCTATAACACCAAC CATGCCCCAAAAGTTCCAGAATCGGCGCGCCAGGCTCTTCGACATGTTCTTCCGCAGCTCCAAGAAACTGCAGGCGGGCT GCCAGAGCCAGAGTTTGCCCACTGAGGTCTAAACGGGGGGTTATGATAACTATACATATAGGAAGGTAAACAAACACTAA ACTTGCTTGCTGAAGAATTCTAAGAACAGTATTCGTAATTTGTAAGTCTAGCAATTAACAAAGACAACTGTTTGAATTTG AGTTTTAAGACCAGATCATGCTGTCCTAGAAGCTTCTCATGCCAAAAAACAAAACCACAAAGAAAGTCAAACTAGAAAGT TTGAGATGCTTTAAATTAACAAAAAAAAAAAAAAAAAAA
Full-length open reading frame fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter.
A cDNA sequence encoding each bait molecule was cloned into the pMK33-C-TAP-FLAG-HA-BD vector as a full-length open reading frame, fused to a carboxyl-terminal FLAG-HA tag cassette under the control of a metallothionein promoter. cDNA sequences are derived from previously characterized and publicly available clones created by the Berkeley Drosophila Genome Project; all inserts are full-length sequence-validated.
